The pathogenesis of equine Osteoarthritis（OA） is more complex, and the disease in the early stage is not easy to be found, therefore, the early diagnosis and treatment are very important. Based on this, this experim...The pathogenesis of equine Osteoarthritis（OA） is more complex, and the disease in the early stage is not easy to be found, therefore, the early diagnosis and treatment are very important. Based on this, this experiment established OA model induced by equine, aimed to study the changes of contents of Matrix Metalloproteinases-3（MMP-3）, Matrix Metalloproteinases-13（MMP-13）, Aggrecanase（ADAMTS-5）, Hyaluronic Acid（HA） and Osteocalcin（OCN） in synovial fluid, and establish rapid diagnostic technique for the equine OA. Thirteen Mongolian equines were used in these induction studies. Equines were randomly divided into two groups： the experimental group contained eight equines and the control group contained five equines. The experimental group was to build the equine osteoarthritis model. The induction was done through Intra-articular（IA） injection of 2 m L Amphotericin-B in equines’ left carpal joints. The equine of the control group was injected into 2 m L physiological saline in equines’ left carpal joints. Synovial fluid was collected every week until the 9th week. The contents of MMP-3, MMP-13, ADAMTS-5, HA and OCN in synovial fluid were evaluated by using ELISA kits. Equine OA model, compared with the control group, starting from the 1st to the 2nd week after induction model, the content of MMP-3, MMP-13, ADAMTS-5, HA and OCN tended to increase, but there was no significant increase, from the 2nd to the 3rd week they significantly increased（p〈0.05） and kept increasing trend until the 9th week. In OA model, MMP-3, MMP-13, ADAMTS-5, HA and OCN showed a rising trend in joint fluid, which would accelerate the cartilage, subchondral bone degradation and metabolism of these proteases increased, and ADAMTS-5 and HA in the early stage increased significantly.展开更多
目的研究膝骨关节炎(osteoarthritis of knee,KOA)滑膜不同部位基质金属蛋白酶3(matrix metalloproteinase-3,MMP-3)的表达强度,探索其与KOA发病机制的关系。方法选取48例KOA患者为研究对象,膝关节镜下滑膜清理术中分别取患侧膝关节内...目的研究膝骨关节炎(osteoarthritis of knee,KOA)滑膜不同部位基质金属蛋白酶3(matrix metalloproteinase-3,MMP-3)的表达强度,探索其与KOA发病机制的关系。方法选取48例KOA患者为研究对象,膝关节镜下滑膜清理术中分别取患侧膝关节内侧间室、髌上囊及外侧间室滑膜,并将患者按综合评分方法进行临床评分。行免疫组织化学染色,比较滑膜不同部位MMP-3的表达强度,并分析MMP-3表达强度与临床评分的相关性。结果①内侧间室滑膜MMP-3表达强度为3.45±0.22,髌上囊滑膜为2.69±0.21,外侧间室为1.99±0.16,差异有统计学意义(P=0.000)。②内侧间室及髌上囊滑膜MMP-3表达强度与临床评分呈正相关关系,相关系数分别为r1=0.894(P=0.000)、r2=0.803(P=0.000);外侧间室滑膜MMP-3表达强度与临床评分无明显相关性r3=0.485(P=0.057)。结论 MMP-3在KOA膝内侧间室滑膜的表达高于滑膜其他部位的表达,与临床表现为膝内侧症状重相符合;膝滑膜病变程度与临床评分呈正相关,为临床上有限切除滑膜病变缓解症状、改善功能提供了依据。展开更多
基金Supported by the National Science and Technology Support Program of China(2012BAD46B02-03)
文摘The pathogenesis of equine Osteoarthritis（OA） is more complex, and the disease in the early stage is not easy to be found, therefore, the early diagnosis and treatment are very important. Based on this, this experiment established OA model induced by equine, aimed to study the changes of contents of Matrix Metalloproteinases-3（MMP-3）, Matrix Metalloproteinases-13（MMP-13）, Aggrecanase（ADAMTS-5）, Hyaluronic Acid（HA） and Osteocalcin（OCN） in synovial fluid, and establish rapid diagnostic technique for the equine OA. Thirteen Mongolian equines were used in these induction studies. Equines were randomly divided into two groups： the experimental group contained eight equines and the control group contained five equines. The experimental group was to build the equine osteoarthritis model. The induction was done through Intra-articular（IA） injection of 2 m L Amphotericin-B in equines’ left carpal joints. The equine of the control group was injected into 2 m L physiological saline in equines’ left carpal joints. Synovial fluid was collected every week until the 9th week. The contents of MMP-3, MMP-13, ADAMTS-5, HA and OCN in synovial fluid were evaluated by using ELISA kits. Equine OA model, compared with the control group, starting from the 1st to the 2nd week after induction model, the content of MMP-3, MMP-13, ADAMTS-5, HA and OCN tended to increase, but there was no significant increase, from the 2nd to the 3rd week they significantly increased（p〈0.05） and kept increasing trend until the 9th week. In OA model, MMP-3, MMP-13, ADAMTS-5, HA and OCN showed a rising trend in joint fluid, which would accelerate the cartilage, subchondral bone degradation and metabolism of these proteases increased, and ADAMTS-5 and HA in the early stage increased significantly.
文摘目的研究膝骨关节炎(osteoarthritis of knee,KOA)滑膜不同部位基质金属蛋白酶3(matrix metalloproteinase-3,MMP-3)的表达强度,探索其与KOA发病机制的关系。方法选取48例KOA患者为研究对象,膝关节镜下滑膜清理术中分别取患侧膝关节内侧间室、髌上囊及外侧间室滑膜,并将患者按综合评分方法进行临床评分。行免疫组织化学染色,比较滑膜不同部位MMP-3的表达强度,并分析MMP-3表达强度与临床评分的相关性。结果①内侧间室滑膜MMP-3表达强度为3.45±0.22,髌上囊滑膜为2.69±0.21,外侧间室为1.99±0.16,差异有统计学意义(P=0.000)。②内侧间室及髌上囊滑膜MMP-3表达强度与临床评分呈正相关关系,相关系数分别为r1=0.894(P=0.000)、r2=0.803(P=0.000);外侧间室滑膜MMP-3表达强度与临床评分无明显相关性r3=0.485(P=0.057)。结论 MMP-3在KOA膝内侧间室滑膜的表达高于滑膜其他部位的表达,与临床表现为膝内侧症状重相符合;膝滑膜病变程度与临床评分呈正相关,为临床上有限切除滑膜病变缓解症状、改善功能提供了依据。
