Background:The purpose of the study was to investigatethe active ingredients and potential biochemicalmechanisms of Simiao Wan(SMW)in obesity-associated insulin resistance.Methods:An integrated network pharmacology me...Background:The purpose of the study was to investigatethe active ingredients and potential biochemicalmechanisms of Simiao Wan(SMW)in obesity-associated insulin resistance.Methods:An integrated network pharmacology method to screen the active compoundsand candidate targets,construct the protein-protein-interaction network,and ingredients-targets-pathways network was constructed for topological analysis to identify core targets and main ingredients.To find the possible signaling pathways,enrichment analysis was performed.Further,a model of insulin resistance in HL-7702 cells was established to verify the impact of SMW and the regulatory processes.Results:An overall of 63 active components and 151 candidate targets were obtained,in which flavonoids were the main ingredients.Enrichment analysis indicated that the PI3K-Akt signaling pathway was the potential pathway regulated by SMW in obesity-associated insulin resistance treatment.The result showed that SMW could significantly ameliorate insulin sensitivity,increase glucose synthesis and glucose utilization and reduce intracellular lipids accumulation in hepatocytes.Also,SMW inhibited diacylglycerols accumulation-induced PKCεactivity and decreased its translocation to the membrane.Conclusion:SMW ameliorated obesity-associated insulin resistance through PKCε/IRS-1/PI3K/Akt signaling axis in hepatocytes,providing a new strategy for metabolic disease treatment.展开更多
Objective:To investigate the expressions of PKC , PKC and PKC in cervical cancer and their relationships with chemosensitivity.Methods: A total of 38 cases of patients with cervical cancer diagnosed in our hospital fr...Objective:To investigate the expressions of PKC , PKC and PKC in cervical cancer and their relationships with chemosensitivity.Methods: A total of 38 cases of patients with cervical cancer diagnosed in our hospital from November 2015 to November 2016 were selected, and the vascularization index (VI), flow index (FI) and vascular flow index (VFI) were measured by three-dimensional color Doppler ultrasound, then the expression levels of PKC , PKC and PKC mRNAs were detected by RT-PCR before and after chemotherapy. Western blot was used to detect the expression of PKC , PKC and PKC proteins in tissues, Spearman correlation analysis was performed to investigate the relationships between PKC , PKC and PKC mRNAs with chemosensitivity, Logisitic regression analysis was used to analyze the related factors affecting the sensitivity of chemotherapy.Results: After chemotherapy, the expression levels of PKC and PKC mRNAs and proteins were significantly decreased, the expressions of PKC mRNA and protein were significantly increased;the expression levels of PKC and PKC mRNAs and proteins in the effective group after chemotherapy were significantly lower than those before chemotherapy, the expressions of PKC mRNA and protein were significantly higher than those before chemotherapy;Spearman analysis showed that the expression levels of PKC and PKC mRNAs in cervical cancer tissues before chemotherapy were positively correlated with VI, FI and the percentage of residual tumor volume after chemotherapy, PKC was negatively correlated with VI, FI and residual tumor volume percentage after chemotherapy, and was not significantly correlated with VFI and tumor size before chemotherapy;Logistic regression analysis showed that PKC , PKC , PKC mRNAs, VI and FI were independent risk factors for chemosensitivity, lymph node metastasis and tumor volume were independent of chemosensitivity.Conclusion: PKC and PKC are highly expressed in cervical cancer tissues, and PKC is low expressed in cervical cancer tissues, they are closely related to chemosensitivity.展开更多
基金supported by the National Natural Science Foundation of China(81903871)Natural Science Foundation of Jiangsu Province(BK20190565)+1 种基金Fundamental Research Funds for the Central Universities(2632021ZD16)Zhenjiang City 2022 Science and Technology Innovation Fund(SH2022084).
文摘Background:The purpose of the study was to investigatethe active ingredients and potential biochemicalmechanisms of Simiao Wan(SMW)in obesity-associated insulin resistance.Methods:An integrated network pharmacology method to screen the active compoundsand candidate targets,construct the protein-protein-interaction network,and ingredients-targets-pathways network was constructed for topological analysis to identify core targets and main ingredients.To find the possible signaling pathways,enrichment analysis was performed.Further,a model of insulin resistance in HL-7702 cells was established to verify the impact of SMW and the regulatory processes.Results:An overall of 63 active components and 151 candidate targets were obtained,in which flavonoids were the main ingredients.Enrichment analysis indicated that the PI3K-Akt signaling pathway was the potential pathway regulated by SMW in obesity-associated insulin resistance treatment.The result showed that SMW could significantly ameliorate insulin sensitivity,increase glucose synthesis and glucose utilization and reduce intracellular lipids accumulation in hepatocytes.Also,SMW inhibited diacylglycerols accumulation-induced PKCεactivity and decreased its translocation to the membrane.Conclusion:SMW ameliorated obesity-associated insulin resistance through PKCε/IRS-1/PI3K/Akt signaling axis in hepatocytes,providing a new strategy for metabolic disease treatment.
文摘Objective:To investigate the expressions of PKC , PKC and PKC in cervical cancer and their relationships with chemosensitivity.Methods: A total of 38 cases of patients with cervical cancer diagnosed in our hospital from November 2015 to November 2016 were selected, and the vascularization index (VI), flow index (FI) and vascular flow index (VFI) were measured by three-dimensional color Doppler ultrasound, then the expression levels of PKC , PKC and PKC mRNAs were detected by RT-PCR before and after chemotherapy. Western blot was used to detect the expression of PKC , PKC and PKC proteins in tissues, Spearman correlation analysis was performed to investigate the relationships between PKC , PKC and PKC mRNAs with chemosensitivity, Logisitic regression analysis was used to analyze the related factors affecting the sensitivity of chemotherapy.Results: After chemotherapy, the expression levels of PKC and PKC mRNAs and proteins were significantly decreased, the expressions of PKC mRNA and protein were significantly increased;the expression levels of PKC and PKC mRNAs and proteins in the effective group after chemotherapy were significantly lower than those before chemotherapy, the expressions of PKC mRNA and protein were significantly higher than those before chemotherapy;Spearman analysis showed that the expression levels of PKC and PKC mRNAs in cervical cancer tissues before chemotherapy were positively correlated with VI, FI and the percentage of residual tumor volume after chemotherapy, PKC was negatively correlated with VI, FI and residual tumor volume percentage after chemotherapy, and was not significantly correlated with VFI and tumor size before chemotherapy;Logistic regression analysis showed that PKC , PKC , PKC mRNAs, VI and FI were independent risk factors for chemosensitivity, lymph node metastasis and tumor volume were independent of chemosensitivity.Conclusion: PKC and PKC are highly expressed in cervical cancer tissues, and PKC is low expressed in cervical cancer tissues, they are closely related to chemosensitivity.
