Chinese soft-shelled turtles （Trionyx sinens） in culture farms using an artificial warming system in Zhejiang, China, often show typical signs of white-spot disease such as white spots on their bodies, skin lesions,...Chinese soft-shelled turtles （Trionyx sinens） in culture farms using an artificial warming system in Zhejiang, China, often show typical signs of white-spot disease such as white spots on their bodies, skin lesions, anorexia and eventually death. The sick turtles were mostly 5-80 g in weight. A suspected fungal pathogen was isolated from the sick turtles and verified as Paecilomyces lilacinus by sequence analysis of the internal transcribed spacer （ITS） of its ribosomal DNA （rDNA）. Detailed morphological examinations were also conducted to confirm the white-spot disease.展开更多
The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, se...The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.展开更多
[Objective] This study was to screen out endophytic fungal strains from the bark of Juglans regia L. [Method] The bark of J. regia was washed with running water and placed on medium to let the fungi grown; the fungal ...[Objective] This study was to screen out endophytic fungal strains from the bark of Juglans regia L. [Method] The bark of J. regia was washed with running water and placed on medium to let the fungi grown; the fungal colonies were studied via morphological observation and anti-microbial activity. [Result] An endophytic fungus showing broad-scope, highly active anti-microbial activity was screened out. This fungus showed anti-microbial activity seven Gram-positive and four Gram-negative bacterial indicators, six skin pathogenic fungi and seven pathogenic fungi to different extent. Morphological observation on the ITS sequence showed that this fungus is consistent with Paecilomyces lilacinus in genus Paecilomyces bainier（with homology of over 99%）. Accordingly this fungus was designated as Paecilomyces lilacinus JY-1024. [Conclusion] This study for the first time reported the isolation of P. lilacinus from J. regia and its in vitro antimicrobial activity to several pathogens.展开更多
基金the Science and Technology Department of Zhejiang Province, China (No. 2004C26026)the Science and Technology Department of Hangzhou City, China (No. 20051322 B33)
文摘Chinese soft-shelled turtles （Trionyx sinens） in culture farms using an artificial warming system in Zhejiang, China, often show typical signs of white-spot disease such as white spots on their bodies, skin lesions, anorexia and eventually death. The sick turtles were mostly 5-80 g in weight. A suspected fungal pathogen was isolated from the sick turtles and verified as Paecilomyces lilacinus by sequence analysis of the internal transcribed spacer （ITS） of its ribosomal DNA （rDNA）. Detailed morphological examinations were also conducted to confirm the white-spot disease.
基金Project(50925417)supported by the National Funds for Distinguished Young Scientist,ChinaProject(2012BAC09B04)supported by the National Key Technology Research and Development Program,China+2 种基金Project supported by the Post-doctoral Program of Central South University,ChinaProjects(31100082,61171061)supported by the National Natural Science Foundation of ChinaProject(2012SK4028)supported by the Science and Technology Program of Hunan Province,China
文摘The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.
基金Supported by Science Research Fund from Yunnan Provincial Education Department(09Y0503)~~
文摘[Objective] This study was to screen out endophytic fungal strains from the bark of Juglans regia L. [Method] The bark of J. regia was washed with running water and placed on medium to let the fungi grown; the fungal colonies were studied via morphological observation and anti-microbial activity. [Result] An endophytic fungus showing broad-scope, highly active anti-microbial activity was screened out. This fungus showed anti-microbial activity seven Gram-positive and four Gram-negative bacterial indicators, six skin pathogenic fungi and seven pathogenic fungi to different extent. Morphological observation on the ITS sequence showed that this fungus is consistent with Paecilomyces lilacinus in genus Paecilomyces bainier（with homology of over 99%）. Accordingly this fungus was designated as Paecilomyces lilacinus JY-1024. [Conclusion] This study for the first time reported the isolation of P. lilacinus from J. regia and its in vitro antimicrobial activity to several pathogens.
