Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe（OP） grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations（0.125,0.250,0....Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe（OP） grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations（0.125,0.250,0.500,1.000 and 2.000 mg/mL） of black tea brew（BTB） were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification.Epigallocatechin gallate（EGCG） was used as the reference agent（concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL）.Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid（from rooster comb）and bovine testicular hyaluronidase.Results:The results revealed that BTB had moderate[IC50=（1.09±0.12） mg/mL]and dose dependent（r2=0.94） anti-hyaluronidase activity.EGCG also exhibited dose dependent（r2=0.93,P【0.05） anti-hyaluronidase activity which was superior[IC50=（0.09±0.00） mg/mL]to BTB.Conclusions:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical.In addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation.展开更多
Objective:To access the pancreatic lipase,cholesterol esterase and cholesterol micellization inhibitory activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea made from uppermost tender leaves and un...Objective:To access the pancreatic lipase,cholesterol esterase and cholesterol micellization inhibitory activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea made from uppermost tender leaves and unopened buds of Camellia sinensis L.Methods:Black tea brew(BTB)was made according to International Organization for Standardization 3103 specifications and concentrations of BTB tested were 37.5,75.0,150.0,300.0 and 600.0μg/mL for antilipase and anti-cholesterol esterase assays and 0.25,0.50 and 1.00μg/mL for cholesterol micellization inhibitory assay.Results:The results showed that BTB of Sri Lankan low grown orthodox Orange Pekoe grade black tea has marked and dose-dependent(r^(2)=0.95)cholesterol micellization inhibitory activity in vitro comparable to epigallocatechin gallate,the reference drug used.In contrast,BTB had only mild but dose-dependent(r^(2)=0.94)inhibitory activity against pancreatic lipase and weak inhibitory effect(up to 13.17%)on pancreatic cholesterol esterase.Conclusions:It is concluded that consumption of BTB of Sri Lankan low grown orthodox Orange Pekoe grade tea as a beverage may be a useful strategy in the management of hyperlipidaemia.展开更多
Objective:To investigate the antibacterial properties of three grades of orthodox Sri Lankan black tea belonging to the three agro-climatic elevations.Methods:Methanloic extracts of orange pekoe(OP),broken orange peko...Objective:To investigate the antibacterial properties of three grades of orthodox Sri Lankan black tea belonging to the three agro-climatic elevations.Methods:Methanloic extracts of orange pekoe(OP),broken orange pekoe fannings(BOPF)and Dust No.1 belonging to three agro-climatic elevations(low,mid and high grown)were made and tested in vitro(concentration:300μg/disc)against Gram-positive pathogenic bacteria,Staphylococcus aureus(ATCC 25923)(S.aureus)and Bacillus cereus(ATCC 11778)(B.cereus),and two Gram-negative bacteria Pseudomonas aeruginosa(ATCC 9027)(P.aeruginosa)and Escherichia coli(ATCC 35218)(E.coli),using agar disc diffusion assay.Gentamycin(10μg/disc)was used as the positive control and methanol as the negative control.Minimum inhibitory concentration(MIC)values were evaluated,using micro dilution method.Results:None of the tea extracts exerted an antibacterial action against P.aeruginosa and E.coli.In contrast mild to moderate antibacterial activity was exerted against S.aureus and B.cereus.Further gentamycin exhibited strong antibacterial activity against all the four bacterial species.Further low MIC values were evident for tea samples against the two Gram-positive bacteria.The order of anti-bacterial activity for tea extracts was Dust No.1>BOPF>OP.Conclusions:It is concluded that Sri Lankan orthodox black tea belonging to Dust No.1,BOPF,and OP pocess in vitro antibacterial activity against S.aureus and B.cereus but not against Gram-positive bacteria P.aeruginosa and E.coli.展开更多
Objective:To investigate the antifungal potential of different grades of Sri Lankan orthodox black tea [orange pekoe, broken orange pekoe fannings (BOPF) and Dust No. 1] belonging to the three agro-climatic elevations...Objective:To investigate the antifungal potential of different grades of Sri Lankan orthodox black tea [orange pekoe, broken orange pekoe fannings (BOPF) and Dust No. 1] belonging to the three agro-climatic elevations (low, mid and high). Methods: Antifungal activity was assessedin vitro using methanolic extracts (300μg/disc) and agar disc diffusion bioassay technique against threeCandida species,Candida albicans (C. albicans),Candida glabrata(C. glabrata), andCandida tropicalis. ketoconazole and itraconazole mixture was used as positive control (10μg/disc) and methanol was used as the negative control. The minimum inhibitory concentrations were also determined using standard protocols. Results: None of the extracts were effective againstCandida tropicalis. Furthermore, orange pekoe grade tea belonging to all agro-climatic elevations did not induce any antifungal activity againstC. albicans andC. glabrata as well. Conversely, Dust No. 1 belonging to all three agro-climatic elevations and low-grownBOPF showed moderate antifungal activity againstC. albicans andC. glabrata. Interestingly, the severity of the antifungal effect varied with agro-climatic elevations. The minimum inhibitory concentrations ranged from 64.00–128.00μg/mL againstC. glabrata and 128.00-256.00μg/mL againstC. albicans. Conclusions:Sri Lankan Dust No. 1 andBOPF have marked antifungal activityin vitro and offer promise to be used as a supplementary beverage in prophylaxis and during drug treatment in candidiasis.展开更多
Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pe...Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black tea.Methods:Freeze dried black tea brew(BTB)was used as the sample in this study.Antiglycation and glycation reversing activity was studied in bovine serum albumin(BSA)-MGO model.Antioxidant properties were studied using total polyphenolic content,total flavonoid content,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant assays.Results:The results demonstrated significant(P<0.05)and dose dependant inhibition of BSA-MGO glycation[IC_(50):(164.30±4.85)μg/mL],potentiating of its reversing activity[EC_(50):(235.39±5.37)μg/mL]and marked antioxidant properties[total polyphenolic content:(119.55±9.97)mg gallic acid equivalents/g BTB;total flavonoid content:(6.04±1.26)mg quercetin equivalents/g BTB;2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power:(3.29±0.06),(1.95±0.15)and(1.31±0.19)mmol Trolox equivalents/g BTB,respectively].No correlations were observed between antioxidant activity and BSA-MGO glycation.Conclusions:The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments.展开更多
基金the National Science Foundation of Sri Lanka for funding and Sri Lanka Tea Board for organoleptic analysis. (NSF/Fellow/2011/01)
文摘Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe（OP） grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations（0.125,0.250,0.500,1.000 and 2.000 mg/mL） of black tea brew（BTB） were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification.Epigallocatechin gallate（EGCG） was used as the reference agent（concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL）.Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid（from rooster comb）and bovine testicular hyaluronidase.Results:The results revealed that BTB had moderate[IC50=（1.09±0.12） mg/mL]and dose dependent（r2=0.94） anti-hyaluronidase activity.EGCG also exhibited dose dependent（r2=0.93,P【0.05） anti-hyaluronidase activity which was superior[IC50=（0.09±0.00） mg/mL]to BTB.Conclusions:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical.In addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation.
基金Supported by National Science Foundation of Sri Lanka(Grant No.NSF/Fellow/2011/01).
文摘Objective:To access the pancreatic lipase,cholesterol esterase and cholesterol micellization inhibitory activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea made from uppermost tender leaves and unopened buds of Camellia sinensis L.Methods:Black tea brew(BTB)was made according to International Organization for Standardization 3103 specifications and concentrations of BTB tested were 37.5,75.0,150.0,300.0 and 600.0μg/mL for antilipase and anti-cholesterol esterase assays and 0.25,0.50 and 1.00μg/mL for cholesterol micellization inhibitory assay.Results:The results showed that BTB of Sri Lankan low grown orthodox Orange Pekoe grade black tea has marked and dose-dependent(r^(2)=0.95)cholesterol micellization inhibitory activity in vitro comparable to epigallocatechin gallate,the reference drug used.In contrast,BTB had only mild but dose-dependent(r^(2)=0.94)inhibitory activity against pancreatic lipase and weak inhibitory effect(up to 13.17%)on pancreatic cholesterol esterase.Conclusions:It is concluded that consumption of BTB of Sri Lankan low grown orthodox Orange Pekoe grade tea as a beverage may be a useful strategy in the management of hyperlipidaemia.
基金Supported by National Science Foundation of Sri Lanka(Grant No.NSF/Fellow/2011/01).
文摘Objective:To investigate the antibacterial properties of three grades of orthodox Sri Lankan black tea belonging to the three agro-climatic elevations.Methods:Methanloic extracts of orange pekoe(OP),broken orange pekoe fannings(BOPF)and Dust No.1 belonging to three agro-climatic elevations(low,mid and high grown)were made and tested in vitro(concentration:300μg/disc)against Gram-positive pathogenic bacteria,Staphylococcus aureus(ATCC 25923)(S.aureus)and Bacillus cereus(ATCC 11778)(B.cereus),and two Gram-negative bacteria Pseudomonas aeruginosa(ATCC 9027)(P.aeruginosa)and Escherichia coli(ATCC 35218)(E.coli),using agar disc diffusion assay.Gentamycin(10μg/disc)was used as the positive control and methanol as the negative control.Minimum inhibitory concentration(MIC)values were evaluated,using micro dilution method.Results:None of the tea extracts exerted an antibacterial action against P.aeruginosa and E.coli.In contrast mild to moderate antibacterial activity was exerted against S.aureus and B.cereus.Further gentamycin exhibited strong antibacterial activity against all the four bacterial species.Further low MIC values were evident for tea samples against the two Gram-positive bacteria.The order of anti-bacterial activity for tea extracts was Dust No.1>BOPF>OP.Conclusions:It is concluded that Sri Lankan orthodox black tea belonging to Dust No.1,BOPF,and OP pocess in vitro antibacterial activity against S.aureus and B.cereus but not against Gram-positive bacteria P.aeruginosa and E.coli.
基金Support by the National Science Foundation of Sri Lanka(Grant NSF/Felow/01/2011).
文摘Objective:To investigate the antifungal potential of different grades of Sri Lankan orthodox black tea [orange pekoe, broken orange pekoe fannings (BOPF) and Dust No. 1] belonging to the three agro-climatic elevations (low, mid and high). Methods: Antifungal activity was assessedin vitro using methanolic extracts (300μg/disc) and agar disc diffusion bioassay technique against threeCandida species,Candida albicans (C. albicans),Candida glabrata(C. glabrata), andCandida tropicalis. ketoconazole and itraconazole mixture was used as positive control (10μg/disc) and methanol was used as the negative control. The minimum inhibitory concentrations were also determined using standard protocols. Results: None of the extracts were effective againstCandida tropicalis. Furthermore, orange pekoe grade tea belonging to all agro-climatic elevations did not induce any antifungal activity againstC. albicans andC. glabrata as well. Conversely, Dust No. 1 belonging to all three agro-climatic elevations and low-grownBOPF showed moderate antifungal activity againstC. albicans andC. glabrata. Interestingly, the severity of the antifungal effect varied with agro-climatic elevations. The minimum inhibitory concentrations ranged from 64.00–128.00μg/mL againstC. glabrata and 128.00-256.00μg/mL againstC. albicans. Conclusions:Sri Lankan Dust No. 1 andBOPF have marked antifungal activityin vitro and offer promise to be used as a supplementary beverage in prophylaxis and during drug treatment in candidiasis.
文摘Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black tea.Methods:Freeze dried black tea brew(BTB)was used as the sample in this study.Antiglycation and glycation reversing activity was studied in bovine serum albumin(BSA)-MGO model.Antioxidant properties were studied using total polyphenolic content,total flavonoid content,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant assays.Results:The results demonstrated significant(P<0.05)and dose dependant inhibition of BSA-MGO glycation[IC_(50):(164.30±4.85)μg/mL],potentiating of its reversing activity[EC_(50):(235.39±5.37)μg/mL]and marked antioxidant properties[total polyphenolic content:(119.55±9.97)mg gallic acid equivalents/g BTB;total flavonoid content:(6.04±1.26)mg quercetin equivalents/g BTB;2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power:(3.29±0.06),(1.95±0.15)and(1.31±0.19)mmol Trolox equivalents/g BTB,respectively].No correlations were observed between antioxidant activity and BSA-MGO glycation.Conclusions:The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments.
