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Outcomes of surgical correction of Peyronie’s disease with plaque excision and grafting: Comparison of testicular tunica vaginalis graft versus bovine pericardium graft
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作者 Ali Eslahi Faisal Ahmed +5 位作者 Mohammad Reza Askarpour Hossein-Ali Nikbakht Iman Shamohammadi Payam Ghasemi Hanieh Alimardani Bahareh Ebrahimi 《Asian Journal of Urology》 CSCD 2024年第3期497-503,共7页
Objective:Peyronie’s disease(PD)is an abnormal wound healing in the penile tunica albuginea.After fibrotic plaque excision,different graft materials have been used to repair the defects,but the optimal graft remains ... Objective:Peyronie’s disease(PD)is an abnormal wound healing in the penile tunica albuginea.After fibrotic plaque excision,different graft materials have been used to repair the defects,but the optimal graft remains unknown.This study aimed to compare the functional outcomes of testicular tunica vaginalis grafts and bovine pericardium grafts in patients with severe PD.Methods:A retrospective comparative study was conducted on 33 PD patients undergoing partial plaque excision and grafting from September 2015 to May 2021.The patients were divided into two groups depending on the type of graft used.For 15 patients in Group B,testicular tunica vaginalis grafts were used to repair the defect,while for 18 patients in Group A,bovine pericardium grafts were used.Data of the patient’s age,comorbidities,sexual function,penile curvature,postoperative complications,need for further treatment,change in penile length,and satisfaction were gathered and compared between the groups.Sexual function was evaluated using the 5-item version of the International Index of Erectile Function(IIEF-5),and a functional less than 20-degree penile curvature after surgery was considered a successful intervention.Results:There was no difference in age,comorbidities,degree of curvature,perioperative IIEF-5,operative time,plaque size,or complication rates.After surgery,a statistically significant improvement in curvature degree(p<0.05)and satisfactory penile appearance(p<0.05)were seen in both groups without any superiority between the two groups(p=0.423 and p=0.840,respectively).With a 30-month follow-up,the IIEF-5 was consistent in both groups,with no statistical significance between the groups(p=0.492).The main change in penile length during the operation was increased and still positive in the last follow-up in both groups without statistical significance(p=0.255 and p=0.101,respectively).Conclusion:Partial plaque excision and corporoplasty with both testicular tunica vaginalis or bovine pericardium grafts are equally effective in treating males with clinically significant PD. 展开更多
关键词 Peyronie's disease Plaque excision Testicular tunica vaginalis graft Bovine pericardium graft
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Congenital Absence of Pericardium:The Largest Systematic Review in the Field on 247 Worldwide Cases(1977-Now)
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作者 Pier Paolo Bassareo Aurelio Secinaro +4 位作者 Paolo Ciliberti Massimo Chessa Marco Alfonso Perrone Kevin Patrick Walsh Colin Joseph Mcmahon 《Congenital Heart Disease》 SCIE 2023年第6期595-610,共16页
Background:Congenital absence of pericardium(CAP),also known as pericardial agenesis,represents an uncommon cardiac abnormality and mostly incidental finding.It can be subdivided into complete and partial(left or righ... Background:Congenital absence of pericardium(CAP),also known as pericardial agenesis,represents an uncommon cardiac abnormality and mostly incidental finding.It can be subdivided into complete and partial(left or right-sided)forms.Because of its infrequency,just case reports and a few case series have been released so far.This paper represents the largest systematic review in the field.Nine features(age at diagnosis,type,gender,clinical presentation,electrocardiography,imaging(ultrasounds,CT/MRI),concomitant cardiac defects,and outcome)were analysed.Methods:The electronic database PubMed was investigated from its establishment up to July 15th,2023.Just case reports and case series were included.Animal studies,papers that were not in English,Spanish,and Italian,and those manuscripts not reporting at least seven of the nine analysed features.were ruled out.The analysed data were reported mostly in terms of percentage.Results:One hundred eighty studies were included encompassing 247 patients.More than half of reviewed CAP cases were in males(63.2%).The mean age at diagnosis was 31.8±19.3 years;a range of 32 weeks of gestation-81 years).23.5%of the patients did not report any symptoms.The most common clinical presentations were chest pain(35.2%)and dyspnoea(29.2%).The most commonly seen ECG changes were right axis deviation(28.7%)and right bundle branch block(23.9%).CAP was suspected or diagnosed by echocardiography in 20.1%of cases.The diagnosis was made by CT and/or MRI in 61.9%of cases.CAP was left-sided in 71.2%,complete in 23.1%,and right-sided in 5.7%.A concomitant congenital heart defect was found in 22.7%,especially in the form of atrial septal defect(6.5%)and patency of ductus arteriosus(2.8%).The pericardial repair was required in 12.9% of the incomplete forms of the disease.Never did the complete form require surgical correction.The outcome appeared favourable in the vast majority of cases,with just 18 deaths(7.3%).Discussion:The main limitation of this systematic review is that it is based just on case reports and case series,due to the lack of large studies on CAP.However,it represents the largest analysis in the field.Due to the rarity of CAP establishing an International Registry is recommended. 展开更多
关键词 Congenital absence pericardium pericardial agenesis ELECTROCARDIOGRAPHY ECHOCARDIOGRAPHY computed tomography cardiac magnetic resonance imaging
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Effect of electroacupuncture at pericardium meridian on D‑ser and NMDAR in acute phase of MCAO rats
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作者 YUAN Liu-mei XIA Yun +3 位作者 LU Xiao-ye LIU Lei ZHOU Ying LOU Bi-dan 《Journal of Hainan Medical University》 2023年第2期16-21,共6页
Objective :To observe the effect of electroacupuncture at pericardium meridian on neurological deficit, D-serine ( D-Ser ) content and NMDAR expression in ischemic brain tissue of rats with middle cerebral artery occl... Objective :To observe the effect of electroacupuncture at pericardium meridian on neurological deficit, D-serine ( D-Ser ) content and NMDAR expression in ischemic brain tissue of rats with middle cerebral artery occlusion ( MCAO ). Methods:A total of 36 SD rats were randomly divided into normal group, sham operation group, model group, EA pericardium meridian group, model+D-Ser group,and EA pericardium meridian+D-Ser group. The MCAO model was prepared by Zea Longa method. The model+D-Ser group and the EA pericardium meridian+D-Ser group were given exogenous D-Ser reagent(160 mg/kg i.p.) 30 minutes after the success of the insertion of the suture. EA pericardium meridian group and EA pericardium meridian+D-Ser group were treated with electroacupuncture for 30 min on the next day. The remaining groups were only bound for 30 min.Behavioral evaluation was performed at 6 h after successful modeling and 2 d after intervention, and then the rats were sacrificed. The content of D-Ser in ischemic brain tissue was measured by liquid chromatography tandem mass spectrometry, and the protein expression of NR1, NR2A and NR2B was detected by Western Blot. Results : Before treatment, compared with the normal group and the sham operation group, the behavioral scores of each group increased after modeling (P<0.05 );Compared with the model group, the behavioral scores of the model+D-Ser group and the EA pericardium meridian+D-Ser group were increased (P<0.05).After treatment, compared with the model group, there was no significant difference in the EA pericardium meridian group (P>0.05),The behavioral scores of the model+D-Ser group and the EA pericardium meridian+D-Ser group were still increased (P<0.05);Compared with the model+D-Ser group, the behavioral score of the EA pericardium meridian+D-Ser group was not statistically significant (P>0.05).Comparison before and after treatment in the group,except for the normal group and the sham operation group, the behavioral scores of each group were slightly increased, and the difference was not statistically significant (P>0.05).Compared with the model group, the content of D-Ser and the protein expression of NR1, NR2A and NR2B in the brain tissue of the model+D-Ser group were significantly increased (P<0.05);The protein expression of NR1, NR2A and NR2B decreased in EA pericardium meridian group (P<0.05);Compared with the model+ D-Ser group, the D-Ser content and the protein expression of NR1, NR2A and NR2B in the EA pericardium meridian+D-Ser group were decreased (P<0.05). Conclusion : EA at pericardium meridian points can reduce the D-Ser content in brain tissue of rats with acute MCAO, regulate the decrease of NR1, NR2A and NR2B protein expression, inhibit the NMDA receptor activity, and play a neuroprotective role. 展开更多
关键词 ELECTROACUPUNCTURE pericardium meridian Middle Cerebral Artery Occlusion D-Ser NR1 NR2A NR2B
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Mechanisms on Correlation between the Pericardium Meridian and Heart Effects of Electroacupuncture at Neiguan and Other Acupo 被引量:1
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作者 Cao Qingshu Liu Junling +2 位作者 Wen Shen Liu Rutting Zhuang Ding(Institute of Acupuncture and Moxibustion, China Academyof Traditional Chinese Medicine, Beijing 100700) 《中国针灸》 CAS CSCD 北大核心 1995年第S2期242-243,共2页
MechanismsonCorrelationbetweenthePericardiumMeridianandHeartEffectsofElectroacupunctureatNeiguanandOtherAcup... MechanismsonCorrelationbetweenthePericardiumMeridianandHeartEffectsofElectroacupunctureatNeiguanandOtherAcupointsonAcuteMyoca... 展开更多
关键词 Acupo Effects HEART MECHANISMS MERIDIAN NEIGUAN OTHER pericardium and at
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Does the Animal Origin Influence the Calcification of Xenograft Tissue Heart Valve Substitutes? Comparison between Bovine and Camel Pericardium in a Subcutaneous Rat Model 被引量:1
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作者 Fatima Al Harmoodi Salah Al Shafy +3 位作者 Michel Guichardant Jean-Jacques Lebrun Francoise Thivolet Olivier Jegaden 《International Journal of Clinical Medicine》 2015年第9期700-709,共10页
Objective: To validate the hypothesis that camel pericardium could be more protected than bovine pericardium against calcification process according to the huge difference in their respective lifestyle and lifetime. M... Objective: To validate the hypothesis that camel pericardium could be more protected than bovine pericardium against calcification process according to the huge difference in their respective lifestyle and lifetime. Methods: Glutaraldehyde (GA) fixed bovine and camel pericardium samples (BP and CP respectively) were both implanted in 30 New Zealand white rats (2 BP and 2 CP matched specimens in each animal) and explanted after 60 days. Unimplanted GA-fixed samples of both species served as control. Matched implanted samples and unimplanted samples were randomly submitted to elemental analysis by spectroscopy, phospholipid extraction, macroscopic and X-ray examination and histology. Results: At 60 days, calcium and phosphorus content were respectively 9.54% ± 3.1% and 4.79% ± 1.4% of tissue dry weight in BP, and 12.52% ± 2.7% and 6.14% ± 1.3% of tissue dry weight in CP (ns). In X-ray analysis, the calcification score was 1.28 ± 0.45 and 2.14 ± 0.98 in BP and CP samples respectively without significant difference (p < 0.08). In histology, calcifications were lower in BP than in CP: 1.37 ± 0.85 vs 2.28 ± 0.83 (ns);collagen fibers were better conserved in BP than in CP: 2.4 ± 0.48 vs 1.87 ± 0.78 (ns), and less disoriented: 25% vs 62% (ns). In unimplanted samples, there was a higher but not significant rate of extracted lipids in CP: 5.7 ± 1.8 vs 9.5 ± 3.8 nanomoles in PS fraction and 11.3 ± 3.7 vs 19 ± 7.7 nanomoles in total fatty acids, in BP and CP samples respectively. All results were in conjunction and demonstrated a higher but not significant rate of mineralization in camel pericardium after implantation, which could be related to a higher but not significant basic rate of phospholipid and fatty acids. Conclusion: This experiment study in a subcutaneous rat model has failed to valid our hypothesis. Because the differences observed between bovine and camel pericardium did not reach the significance, at the best, there is no difference between both species and at the worst, camel pericardium has a higher rate of the phosphatidylserine fraction of phospholipid, and is more sensitive and prompt to calcification. 展开更多
关键词 pericardium CALCIFICATION BOVINE CAMEL XENOGRAFT Tissue Heart Valve SUBSTITUTES
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Use of bovine pericardium graft for abdominal wall reconstruction in contaminated fields
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作者 Luigi D’Ambra Stefano Berti +3 位作者 Cosimo Feleppa Prospero Magistrelli Pierfrancesco Bonfante Emilio Falco 《World Journal of Gastrointestinal Surgery》 SCIE CAS 2012年第7期171-176,共6页
AIM:To employ, in such conditions, a biological graft such as bovine pericardium that offers resistance to infection. METHODS:In our surgical department, from January 2006 to June 2010, 48 patients underwent abdominal... AIM:To employ, in such conditions, a biological graft such as bovine pericardium that offers resistance to infection. METHODS:In our surgical department, from January 2006 to June 2010, 48 patients underwent abdominal wall reconstruction using acellular bovine pericardium; of these 34 patients had a contaminated wound due to diffuse peritonitis (complicated diverticulitis, bowel perforation, intestinal infarction, strangled hernia, etc. ) and 14 patients had hernia relapse on infected synthetic mesh. RESULTS: In our series, one patient died of multiorgan failure 3 d after surgery. After placement of the pericardium mesh four cases of hernia relapse occurred. CONCLUSION: Recurrence rate is similar to that of prosthetic mesh repair and the application of acellular bovine pericardium (Tutomesh , Tutogen Medical Gmbh Germany) is moreover a safe and feasible option thatcan be employed to manage complicated abdominal wall defects where prosthetic mesh is unsuitable. 展开更多
关键词 BOVINE pericardium ABDOMINAL wall Contaminated field ABDOMINAL HERNIA HERNIA RELAPSE
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Primary primitive neuroectodermal tumor in the pericardium-a focus on imaging findings:A case report
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作者 Shu-Ming Xu Juan Bai Jin-Hua Cai 《World Journal of Clinical Cases》 SCIE 2021年第17期4336-4341,共6页
BACKGROUND Primitive neuroectodermal tumors(PNETs)are rare,sporadic malignant tumors of the peripheral nervous system,bone,or soft tissues.However,to the best of our knowledge,only three cases of PNET in the pericardi... BACKGROUND Primitive neuroectodermal tumors(PNETs)are rare,sporadic malignant tumors of the peripheral nervous system,bone,or soft tissues.However,to the best of our knowledge,only three cases of PNET in the pericardium have been reported in the English literature,and their magnetic resonance imaging findings have not previously been described.CASE SUMMARY A 3-year-old boy was hospitalized with a 1-wk history of recurrent vomiting and weakness.Detailed history-taking revealed no evidence of heart disease.Computed tomography demonstrated a soft tissue mass in the left pericardial cavity with heterogeneous contrast enhancement.The border between the mass and the heart was poorly defined.Thoracotomy revealed a mass invading the left ventricle,with a high risk of bleeding.The mass was considered inoperable.A biopsy was performed,and the histological and immunohistochemical findings confirmed the diagnosis of primary PNET of the pericardium.The patient received four cycles of standard chemotherapy.Chest magnetic resonance imaging 3 mo after the initiation of chemotherapy revealed that the tumor in the pericardium still existed,but its volume had slightly decreased.The patient was lost to follow-up,and the final outcome was therefore unknown.CONCLUSION Medical imaging plays an important role in defining the pericardial origin of PNET and understanding its characteristics.Magnetic resonance imaging can provide more information on the tumor than computed tomography and may thus aid therapeutic planning. 展开更多
关键词 Primary primitive neuroectodermal tumors pericardium Tumor Computed tomography Magnetic resonance imaging Case report
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Valve Repair Using Autologous Pericardium in Tricuspid Valve Endocarditis
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作者 Kuldeep Singh Mingli Wang +1 位作者 Sean A. Marco David D’Alessandro 《World Journal of Cardiovascular Surgery》 2012年第2期7-9,共3页
Tricuspid valve endocarditis is a significant cause of valve-related morbidity and mortality. Prosthetic valve reconstruction can be costly and requires life-long anticoagulation. We present our experience with surgic... Tricuspid valve endocarditis is a significant cause of valve-related morbidity and mortality. Prosthetic valve reconstruction can be costly and requires life-long anticoagulation. We present our experience with surgical management of an endocarditis damaged tricuspid valve leaflet in an intravenous drug user. We were able to excise the infected tissue and successfully repair the native valve using autologous pericardium, effectively eliminating the need for prostheses and associated complications. Our case report demonstrates that tricuspid valve reconstruction, using autologous pericar-dium, is a suitable option in patients with tricuspid endocarditis. 展开更多
关键词 CARDIAC ENDOCARDITIS Heart VALVE AUTOGRAFT pericardium TRICUSPID VALVE
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Primary synovial sarcoma of pericardium: A case report
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作者 Rangreze Imran Banday Manzoor Ahmad +2 位作者 Sheikh Aejaz Aziz Bhat Salma Mohammad Latif Charoo 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第7期428-429,共2页
Primary pericardial sarcomas are extremely rare. We report a case of 19 year old male who presented with cough, dyspnoea, and orthopnea. Investigations and exploratory thoracotomy revealed a large pericardial mass. Su... Primary pericardial sarcomas are extremely rare. We report a case of 19 year old male who presented with cough, dyspnoea, and orthopnea. Investigations and exploratory thoracotomy revealed a large pericardial mass. Surgical debulking of the tumor was performed and the histopathological examination was compatible with synovial sarcoma. The tumor was unresectable due to its invasion and adhesion to mediastinal structures. Hence patient was started on palliative chemotherapy (adriamycin and ifosfamide based). Patient showed an initial symptomatic response but later on there was a clinical progres-sion and died within six months of his diagnosis. 展开更多
关键词 synovial sarcoma pericardium MRI
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A canine model of aortic arch aneurysm created with autologous pericardium
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作者 Weixiao Li Jun Li +7 位作者 Qiang li Mingzhe Cui Rutao Xu Shuiting Zhai Cheshire Nick Tianxiao Li Jiangbo Chen Wenli Zhao 《Journal of Interventional Medicine》 2022年第3期133-137,共5页
Background:To establish a canine model of aortic arch aneurysm that is suitable for research on new devices and techniques applied to the aortic arch.Materials and methods:Fifteen mongrel dogs underwent surgery.The au... Background:To establish a canine model of aortic arch aneurysm that is suitable for research on new devices and techniques applied to the aortic arch.Materials and methods:Fifteen mongrel dogs underwent surgery.The autologous pericardial patch was sewn on the aortotomy site in the anterior wall of the aortic arch.The animals were followed up for 3 months postoperatively.Computed tomography angiography was used to visualize and measure the aneurysm model.Hematoxylin and eosin staining was used to observe the histological characteristics of the aneurysm model.Changes in aneurysm diameter over time were analyzed using analysis of variance.Results:One dog died of hemorrhage during surgery.Fourteen dogs survived the surgical procedure.Two of them died on the first postoperative day because of ruptures at the suturing margin.The diameter of the aneurysm model was twice as large as that of the aortic arch.There was no significant change in the maximum diameter of the aneurysm model during the follow-up period.Conclusions:We established a controllable and stable aortic arch aneurysm model created with an autologous pericardium patch.The aneurysm model can be used to research endoleaks after thoracic endovascular aortic repair and new endovascular techniques can be applied to the aortic arch. 展开更多
关键词 Aortic aneurysm pericardium patch Ascending aorta Aortic arch Animal model
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Aortic Neocuspidization with Autologous Pericardium: Initial Experience of Single Center
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作者 Kubilay Karabacak Emre Kubat +8 位作者 Gökhan Erol Murat Kadan Furkan Burak Akyol Elgin Hacızade Suat Doganci Mehmet Emin Ince Uygar Cagdas Yuksel Murat Celik Cengiz Bolcal 《World Journal of Cardiovascular Surgery》 2021年第6期51-60,共10页
<strong>Background:</strong><span style="font-family:;" "=""> Aortic neo-cuspidization (AVNeo) procedure has been adopted by limited centers with </span><span style=... <strong>Background:</strong><span style="font-family:;" "=""> Aortic neo-cuspidization (AVNeo) procedure has been adopted by limited centers with </span><span style="font-family:;" "="">the </span><span style="font-family:;" "="">publication of the mid-long term successful resu<span>lts. The aim of this study was to present initial experience of the AVNeo pro</span>cedure of </span><span style="font-family:;" "="">a </span><span style="font-family:;" "="">single center.</span><span style="font-family:;" "=""> </span><b><span style="font-family:;" "="">Methods:</span></b><span style="font-family:;" "=""> The medical records of 24 patients who underwent AVNeo with or without concomitant cardiac surgery between February 2019 and February 2021 at our tertiary hospital were scanned retrospectively. <b>Results:</b> The mean age of patients was aged 58.21 ± 13.14 years an<span>d 16 (66.7%) of them were men. 16 patients were operated </span></span><span style="font-family:;" "="">on </span><span style="font-family:;" "="">for aortic steno</span><span style="font-family:;" "="">sis (66.67%). Morphology of the aortic valve was tricuspid in 21 (87.5%) and bi<span>cuspid in 3 (12.5%) of the patients. Additional cardicac surgery was performed </span>in 13 (54.17%) patients. No patients needed reoperation for bleeding, pace<span>maker implantations, conversion to classical prosthetic aortic valve replacement</span></span><span style="font-family:;" "="">,</span><span style="font-family:;" "=""> or infective endocarditis. Two patients died due to non-cardiac reasons</span><span style="font-family:;" "="">. Preoperative peak and mean aortic valve pressures improved significantly at 1<sup>st</sup> and 6<sup>th</sup> months (Preop:</span><span style="font-family:;" "=""> </span><span style="font-family:;" "="">89.06 ± 21.88 mmHg and 56.38 ± 15.09 mmH<span>g 1<sup>st</sup> month: 22.00 ± 3.93 mmHg and 8.73 ± 2.60 mmHg, 6<sup>th</sup> month: 18.13 </span>± 3.02 mmHg and 6.93 ± 1.83 mmHg)</span><span style="font-family:;" "="">. </span><b><span style="font-family:;" "="">Conclusion:</span></b><span style="font-family:;" "=""> In conclusion, the AVNe<span>o procedure is a feasible technique for aortic valve pathologies, with the adv</span>antages of avoiding anticoagulants and the applicability of concomitant surgic<span>al procedures. Although this procedure requires meticulous experience, res</span>ults similar to the available published literature can be obtained and reproducible even during learning curve when technical steps are strictly followed.</span> 展开更多
关键词 Ozaki Procedure Aortic Valve REPAIR Autologous pericardium
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Comparative Study of Bovine Pericardium and Gore-Tex in Tissual Interaction with Wistar Rats Diaphragm
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作者 Artur Fracassi Guerra Mauro Razuk Filho +1 位作者 Neil Ferreira Novo Willy Marcus França 《Surgical Science》 2016年第9期381-389,共10页
Introduction: Simple suture isn’t always possible in large congenital diaphragmatic hernia (cdh) defects. Synthetic materials are used for correction such as Silastic&reg, Gore-Tex&reg (GT), Teflon&reg or... Introduction: Simple suture isn’t always possible in large congenital diaphragmatic hernia (cdh) defects. Synthetic materials are used for correction such as Silastic&reg, Gore-Tex&reg (GT), Teflon&reg or biological, such as autologous muscle patches. It was shown that bovine pericardium (bp) was effective to correct those large defects with many positive outcomes when compared with syntactic materials. Aim: This study aims to establish an experimental model of correction for large diaphragmatic defect with PB and GT patches to compare histologically the tissue interaction between them and diaphragm in young Wistar rats. Materials & Methods: 15 wistar rats were divided in 3 groups: Rats that used BP was named G1;Rats that used GT was named G2;and rats with only scraping in the diphragm, named G3 (control). Animals were submited to a laparotomy and fixed pathces to diaphragms and harvested 3 weeks later. Area between normal diaphragm and patches were isolated and separated for histological analysis, such as lymphocytic infiltration (inflammation), neovascularization and fibrosis. Results: G1 presented inflammation between BP and Diaphragm In 5 Samples. G2 Presented Neovascularization In 5 Samples, But No inflammation. Fibrotic tissue overlapping GT patches occurred in 3 samples in G2. Comparing G1 with G2 there was a significant statistical difference concerning inflammation (P = 0.0079), in G1. Comparing neovascularization there is no significant statistical difference (P = 0.4444), despite a slight higher incidence in G2. Fibrosis in both groups presented no significant statistical difference (P = 0.4444), despite a slight higher incidence in G2. There were no alterations in G3. Discussion: Despite the statistical difference in the inflammatory process was more frequent in G1 (P = 0.0079), neovascularization and fibrosis were more frequent in G2. Conclusion: The proposed experimental model was satisfactory to reproduce suture of patches in the diaphragm. These results suggests that inflammation, neovascularization and fibrosis indeed contribute to a benign healing process that reacts differently in each group but can drive to a more lasting and permanent results when biological patch is considered. Statistical report suggests that this study should be continued with a larger sample of animals and a wider period of time before harvest. 展开更多
关键词 Diaphragm Hernia Bovine pericardium GORE-TEX Tissue Interaction and Wistar Rat
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Comparative Efficacy of Different Inactivated Hydro-Pericardium Syndrome Vaccines Prepared from Infected Liver and Vero Cell Line Adapted Adeno Type 4 Virus
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作者 Muhammad Danish Mehmood Huma Anwarul-Haq +5 位作者 Faisal Amin Sajjad Hussain Ejaz Rafique Muhammad Usman Ghani Muhammad Ismail Fareeha Ghaffar 《World Journal of Vaccines》 2020年第1期1-16,共16页
Hydro-Pericardium Syndrome (HPS) is viral problem of commercial poultry caused by aviadeno virus type-4. In Pakistan the problems have been controlled by administering inactivated infected liver homogenate vaccine (IL... Hydro-Pericardium Syndrome (HPS) is viral problem of commercial poultry caused by aviadeno virus type-4. In Pakistan the problems have been controlled by administering inactivated infected liver homogenate vaccine (ILHV). The use of liver based HPS vaccines remained potential threat for having hypersensitivity reactions in poultry. The current study was carried out to compare the serological potency of HPS ILHV to vero cell line adopted vaccine in term of anti HPS-ELISA antibody titers. 14 HPS virus vaccines were prepared based on different concentration of antigen, type of adjuvants and source of virus substrate. Total of 160 birds were divided into 16 groups each containing 10 birds. At day of 14th age each bird of every group was injected with 0.3 ml dose of respective vaccine. It was observed that HPS infected liver based vaccine having 1 × 105.6, 1 × 105.6 and 1 × 103.6 bird lethal dose 50 induced 1092.10, 875.25 and 702.2 anti-HPS ELISA antibody titer respectively. The 20, 25 and 30 doses/gm HPS infected liver vaccine induced 110.4, 1071.9 and 1037.8 anti-HPS ELISA antibody titer respectively. Montanide based tissue culture HPS vaccine showed significantly higher 1148.45 anti-HPS ELISA antibody titer to aluminium hydroxide based vaccine (137.2) (P 5.6 TCID50 is serological potent against field infection. The vaccines based on such formulation could be prepared in future for effective immuno-prophylaxis against HPS virus. 展开更多
关键词 Hydro-pericardium SYNDROME VERO Cell Line INFECTED LIVER Homogenate Cytopathic Effect Tissue Culture Infective Dose Montanide HPS ELISA
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Rebar Impalement Injury Throughout Bilateral Hemithorax and Pericardium
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作者 Tevfik Ilker Akcam Ayse Gul Ergonul +2 位作者 Ali Ozdil Alpaslan Cakan Ufuk Cagirici 《Open Journal of Thoracic Surgery》 2015年第3期31-34,共4页
Thoracic traumas have an important place among all injuries as they can cause significant mortality and morbidity. The thoracic injuries are the large part of trauma with head and extremity injuries. Thoracic traumas ... Thoracic traumas have an important place among all injuries as they can cause significant mortality and morbidity. The thoracic injuries are the large part of trauma with head and extremity injuries. Thoracic traumas are divided into two groups, including penetrating and blunt. The primary causes for penetrating traumas are wounds caused by gunshot and sharp objects. The lungs are the most injured organ in penetrating thoracic traumas with many organ injuries accompanied. This case report is about a rebar impalement into bilateral hemitoraces and pericardium after falling down in spite of any injury is not occurred in heart, lung and great vessels. The fact that an injury to such an extent does not cause any intrathoracic catastrophic event is something that has not been reported in the past. 展开更多
关键词 REBAR INJURY Hemithoraces pericardium
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Study on the Preparation of Decellularized Scaffold of Bovine Pericardium
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作者 YAN Fei-yan WEI Yi-han LIU Xin GU Han-qing 《Chinese Journal of Biomedical Engineering(English Edition)》 2010年第3期129-138,共10页
Objective: To search for the best procedure on preparation ofacellular bovine pericardium,so to provide scaffolds for constructing tissue-engineering Methods: The bovine pericardiums were treated with 5 methods, whi... Objective: To search for the best procedure on preparation ofacellular bovine pericardium,so to provide scaffolds for constructing tissue-engineering Methods: The bovine pericardiums were treated with 5 methods, which were divided into 6 groupsGroup A: Fresh bovinepericardium; Group B: Trypsin-detergent group;Group C: Freeze-thaw-detergent 24 h group; Group I): Freeze-thaw-detergent 48 h group; Group Fz Freeze-thaw-nuclease group; Group F: Detergent-nuclease group. Then, by HE staining and scanning electron microscope to observe the effects ofdecellularization and fibrous changes among the 6 groups; by water content testingonechanical testing to observe the changes in physical properties of the matrix; by detecting the DNA content of each group to determine the effect of deeellularization qualitatively; by eytotoxieity test to detect the biocompatibility of bovine pericardium in each group. Results: The 5 methods can all remove the cellular components effectively, compared with the fresh bovine pericardium,the water content of each deeellularized group were increased (P〈0.05), while the DNA content decreased (P〈0.05), with statistically significant differences. Of group E, the fibers were a little disorder, with the largest tension and the elastic modulus increased, while the rupture tensile rate decreased. Compared with fresh bovine pericardium,the largest tension of the other decellularization groups were all decreased (P〈0.05). The fibers of group B, group D were irregularly arranged and also with ruptures, both the elastic modulus and the rupture tensile rate decreased(P〈0.05). In group C and F, the fibers were dense and their direction was normal, the elastic modulus and the rupture tensile rate were similar to the fresh bovine pericardium (P〉0.05). Cytotoxicity results showed that the cell toxicity of group B, group C, group D, group E and group F were respectively 0.9, 0.6, 1.0, 1.0 and 0.5, each group were qualified toxicity test, in which group C and group F were with the lowest eytotoxieity. Conclusion: Group C and group F can remove the cell components of bovine pericardium successfully,while maintaining the major structural components and the histological and biological properties of bovine pericardium,and with low cytotoxicity.However, group C is more economical than group F,and easier to operate. So the method on freeze-thaw-detergent 24 h can be the best choice to produce a decellularized bovine pericardium. 展开更多
关键词 bovine pericardium deeellularization freeze-thaw method SCAFFOLD DETERGENT
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Study on Characteristics of Acellular Bovine Pericardium Crosslinked with Genipin
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作者 TIAN Cong WAN Rong-xin +1 位作者 GU Han-qing LIU Xin 《Chinese Journal of Biomedical Engineering(English Edition)》 2011年第1期10-18,共9页
The study used a naturally occurring crosslinking reagent-genipin to chemically modify acellular bovine pericardium, prepare cardiac valve tissue engineering scaffold material,and evaluated genipin crosslinked acellul... The study used a naturally occurring crosslinking reagent-genipin to chemically modify acellular bovine pericardium, prepare cardiac valve tissue engineering scaffold material,and evaluated genipin crosslinked acellular matrix of bovine pericardium by investigating the physical and chemical properties of the tissues, such as the surface properties, crosslinking characteristics, mechanical properties, resistance to enzymatic capacity in vitro, and hemolysis tests. The results showed that acellular bovine pericardium matrix erosslinked with genipin was strong hydrophilicity, high crosslinking index, and stable structure, which can maintain good mechanical properties. As a kind of scaffold material for valve tissue engineering, it has wide application prospect. 展开更多
关键词 acellular bovine pericardium GENIPIN GLUTARALDEHYDE crosslinking characteristics mechanical properties
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Reconstruction of pulmonary artery by autologous pericardium or azygous venae for non-small cell lung cancer
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作者 刘学刚 《外科研究与新技术》 2011年第3期158-159,共2页
Objective To review the clinical experience of reconstruction of pulmonary artery(PA) by a patch of autologus pericardium or azygous venae for non-small cell lung cancer. Methods Between March 1992 and August 2009,62 ... Objective To review the clinical experience of reconstruction of pulmonary artery(PA) by a patch of autologus pericardium or azygous venae for non-small cell lung cancer. Methods Between March 1992 and August 2009,62 patients with locally advanced central lung cancer received sleeve resection and reconstruction of PA. 展开更多
关键词 LUNG Reconstruction of pulmonary artery by autologous pericardium or azygous venae for non-small cell lung cancer CELL
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Complex reimplantation of an inflatable penile prosthesis with scar tissue excision and distal corporal defect repair with Tutoplast®pericardium allograft
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作者 Kyle A.Blum William Jones +3 位作者 Lauren Conroy Justin Mehr Travis Green Run Wang 《UroPrecision》 2023年第2期80-83,I0006,共5页
Background:Patients with inflatable penile prostheses(IPP)may experience erosions after years of use or after indwelling Foley catheter placement.IPP erosions should be managed as an infection with the entire IPP remo... Background:Patients with inflatable penile prostheses(IPP)may experience erosions after years of use or after indwelling Foley catheter placement.IPP erosions should be managed as an infection with the entire IPP removed.Replacing IPPs in erosion patients can be challenging due to extensive scarring development.Using proper graft materials may be necessary to close and reinforce the corporal defect after difficult corporal dilation or significant scar removal for the long‐term success of IPP reimplantation.Case Presentation:Herein we report a patient with a history of IPP placement in 2007 for erectile dysfunction and Peyronie's disease,presenting in 2018 with dysuria and a feeling of IPP displacement following Foley catheter placement for coronary artery bypass graft surgery.The left cylinder of the IPP was observed by physical examination and a defect near the fossa navicularis was discovered during office cystoscopy.A complete explantation of IPP was then performed.Reimplantation of the IPP was performed in 2021 which required excision of corporal scar tissue and repair of a left distal corporal defect using a Tutoplast®pericardium allograft.The procedure was successful with proper IPP position and good function without any penile deformity.Conclusion:The use of graft materials in the management of corporeal defects should be considered in the setting of complex IPP revision.The Tutoplast®pericardium allograft reinforcement can provide long‐term durability,flexibility,and a lower risk of infection. 展开更多
关键词 corporal erosion distal corporal perforation inflatable penile prosthesis Peyronie's disease Tutoplast®pericardium allograft
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电针心经/心包经对阿尔茨海默病模型大鼠记忆能力及Aβ1-42蛋白表达的影响
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作者 盛蓉蓉 苏汝欣 +6 位作者 曹洋 柯超 谭艳 潘江 石文英 章薇 魏歆然 《陕西中医》 CAS 2024年第11期1443-1448,共6页
目的:探讨电针心经/心包经对阿尔茨海默病大鼠学习记忆能力及心脏HO-1、Aβ1-42表达的影响。方法:选用SD大鼠35只,随机分为正常组、假手术组、模型组、心包经组、心经组,每组7只。分别对模型组、心包经组、心经组大鼠采用双侧海马内注射... 目的:探讨电针心经/心包经对阿尔茨海默病大鼠学习记忆能力及心脏HO-1、Aβ1-42表达的影响。方法:选用SD大鼠35只,随机分为正常组、假手术组、模型组、心包经组、心经组,每组7只。分别对模型组、心包经组、心经组大鼠采用双侧海马内注射0.9%氯化钠溶液和Aβ1-42法建立AD模型。成模后对心包经组、心经组进行电针干预,正常组、假手术组、模型组捆绑处理,不进行电针刺激。治疗结束后运用Morris水迷宫检测大鼠学习记忆能力、HE染色法观察大鼠心肌组织病理改变、免疫组化法和免疫印迹法检测大鼠心肌HO-1和Aβ1-42蛋白表达情况。结果:假手术组各项指标与正常组比较,差异无统计学意义(均P>0.05)。与假手术组比较,模型组大鼠的逃避潜伏期明显延长,第二象限百分比、跨越平台次数减少,差异有统计学意义(均P<0.05)。心肌纤维排列紊乱,炎症反应明显,HO-1蛋白表达下调,而Aβ1-42蛋白表达增加,差异有统计学意义(均P<0.05)。与模型组比较,心经组、心包经组大鼠各项异常指标均得到改善,差异有统计学意义(均P<0.05)。与心经组比较,心包经组的逃避潜伏期缩短,第二象限百分比和跨越平台次数增加,差异有统计学意义(均P<0.05)。心经组HO-1与Aβ1-42蛋白表达与心包经组比较,差异无统计学意义(P>0.05)。结论:电针心经/心包经可改善阿尔茨海默病大鼠空间学习记忆能力,其作用机制可能与上调HO-1表达,减轻Aβ1-42神经毒性损伤有关。 展开更多
关键词 阿尔茨海默病 电针 心经 心包经 血红素氧化酶1 Β淀粉样蛋白
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产前超声诊断胎儿心包畸胎瘤1例
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作者 何予姝 马斌 +3 位作者 潘婷 王刚 张敏红 王颜曌 《中国医学影像技术》 CSCD 北大核心 2024年第4期602-602,共1页
孕妇29岁,孕1产0,孕23周产前超声提示胎儿胸腔占位;既往无特殊病史及家族史。孕23周胎儿超声:于三血管气管切面探及大血管旁1.3cm×1.2cm略高回声包块,形态规则,向上延伸至主动脉与上腔静脉间隙内,四腔心切面及矢状切面见包块向下... 孕妇29岁,孕1产0,孕23周产前超声提示胎儿胸腔占位;既往无特殊病史及家族史。孕23周胎儿超声:于三血管气管切面探及大血管旁1.3cm×1.2cm略高回声包块,形态规则,向上延伸至主动脉与上腔静脉间隙内,四腔心切面及矢状切面见包块向下达右心房后方(图1A);CDFI于包块内未见明显血流信号,于室间隔膜周部探及宽2mm过隔血流束,心室水平见右向左分流(图1B)。超声诊断:心包畸胎瘤可能性大;可疑室间隔缺损。孕妇选择引产。胎儿标本心脏右后方见1.4cm×1.2cm×1.0cm包块,形态规整,边界清晰(图1C). 展开更多
关键词 胎儿 畸胎瘤 心包 超声检查
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