The aqueous extract of Phellodendron amurense Rupr.(Amur Cork Tree) provides a rich source of antioxidants and chemical compounds, and can be used for food and wood preservative materials.In this study, we characteriz...The aqueous extract of Phellodendron amurense Rupr.(Amur Cork Tree) provides a rich source of antioxidants and chemical compounds, and can be used for food and wood preservative materials.In this study, we characterized the chemical composition of this extract by GC and GC/MS.The antioxidant capacity was evaluated using a variety of antioxidant assays(superoxide radical,hydroxyl radical, nitric oxide radical, and DPPH radical scavenging activity).Additionally, total polyphenolic content was determined.Phenolic acids and acetone derivatives were major compounds of the extract capable of scavenging the DPPH free radical and reducing ferric ions.DPPH and ferric ion reduction results were strongly correlated with total phenolic content of the extract which also exhibited strong nitric oxide, hydroxyl radical scavenging and superoxide anion radical scavenging activities.展开更多
Palmatine is a valuable ingredient in Chinese medicine that is produced by Phellodendron amurense Rupr.The contents of palmatine content in root bark, trunk bark,perennial branch bark, annual branches, and leaves of t...Palmatine is a valuable ingredient in Chinese medicine that is produced by Phellodendron amurense Rupr.The contents of palmatine content in root bark, trunk bark,perennial branch bark, annual branches, and leaves of the trees with different ages and geographies in Northeast China were measured by high-performance liquid chromatography.The contents of palmatine in the barks of root, trunk, and perennial branch were significantly higher than those in annual branches and leaves. The contents of palmatine in trunk bark and root bark from Lesser Khingan Mountains increased with age, which is significantly opposite to other three vegetation types. The contents of palmatine in perennial branch bark, annual bark and leaves had no significant regularity. Moreover, the contents of palmatine in samples of root bark, trunk bark, perennial branch bark and annual bark varied significantly with latitude. The nature populations of P. amurense growing at low latitude contained significantly more palmatine than those growing at high latitude. These results provide a scientific basis for the reasonable cultivation and efficient utilization of P. amurense.展开更多
Phellodendron amurense has been used for many years as a medical plant in traditional Chinese medicine and has shown great prospect in recent clinical trials for future applications. Berberine is an essential active c...Phellodendron amurense has been used for many years as a medical plant in traditional Chinese medicine and has shown great prospect in recent clinical trials for future applications. Berberine is an essential active compound contained in P. amurense. Our objective in this study was to quantify the content of berberine in P.amurense from sites at different elevations on Changbai Mountain. We collected samples of P. amurense from five different elevations on Changbai Mountain. Berberine in samples was extracted by ultrahigh pressure extraction(UPE). And the quantity was measured by high performance liquid chromatography(HPLC). First, the optimal HPLC conditions for berberine were identified with satisfactory precision(relative standard deviation, R SD\5.6 %), good accuracy(relative error, R E\ 3.6 %) and good linear relation(R2= 0.9998) in the range of 6.576–328.8 mg L-1. Second, the combination of UPE and HPLC methods in quantitative analysis of berberine showed high repeatability(R SD= 3.28 %), reproducibility(R SD= 4.72 %),stability(R SD\ 1.27 %) and good recovery(99.54 %) for real plant materials. Samples from Heilongjiang Province at the lowest elevation contained the highest amount of berberine.Similarly, the lowest amount of berberine was recorded in samples from Changbai Forest Bureau of Jilin Province collected at the highest elevation in this paper. The proposed UPE–HPLC method is simple, reliable and low-cost for quantitative analysis of berberine. Content of berberine in P.amurense varied significantly by site on Changbai Mountain.展开更多
[Objectives] This study was conducted to study the anti-gout effect of Phellodendron amurense and its different processed products. [Methods] Extracts of P. amurense and its different processed products were fed to ra...[Objectives] This study was conducted to study the anti-gout effect of Phellodendron amurense and its different processed products. [Methods] Extracts of P. amurense and its different processed products were fed to rats respectively,to observe uric acid and creatinine in the serum and swelling index. [Results] Compared with the data of positive control,P. amurense and the processed products could significantly reduce the high uric acid and creatinine in the serum,and also could resist the swelling in rats. [Conclusions] P. amurense and its processed products showed different degrees of anti-gout effect,and the processing methods were found to have no remarkable impact on this effect.展开更多
文摘The aqueous extract of Phellodendron amurense Rupr.(Amur Cork Tree) provides a rich source of antioxidants and chemical compounds, and can be used for food and wood preservative materials.In this study, we characterized the chemical composition of this extract by GC and GC/MS.The antioxidant capacity was evaluated using a variety of antioxidant assays(superoxide radical,hydroxyl radical, nitric oxide radical, and DPPH radical scavenging activity).Additionally, total polyphenolic content was determined.Phenolic acids and acetone derivatives were major compounds of the extract capable of scavenging the DPPH free radical and reducing ferric ions.DPPH and ferric ion reduction results were strongly correlated with total phenolic content of the extract which also exhibited strong nitric oxide, hydroxyl radical scavenging and superoxide anion radical scavenging activities.
基金supported by Special Fund for ForestryScientific Research in the Public Interest(Grant No.201104066)
文摘Palmatine is a valuable ingredient in Chinese medicine that is produced by Phellodendron amurense Rupr.The contents of palmatine content in root bark, trunk bark,perennial branch bark, annual branches, and leaves of the trees with different ages and geographies in Northeast China were measured by high-performance liquid chromatography.The contents of palmatine in the barks of root, trunk, and perennial branch were significantly higher than those in annual branches and leaves. The contents of palmatine in trunk bark and root bark from Lesser Khingan Mountains increased with age, which is significantly opposite to other three vegetation types. The contents of palmatine in perennial branch bark, annual bark and leaves had no significant regularity. Moreover, the contents of palmatine in samples of root bark, trunk bark, perennial branch bark and annual bark varied significantly with latitude. The nature populations of P. amurense growing at low latitude contained significantly more palmatine than those growing at high latitude. These results provide a scientific basis for the reasonable cultivation and efficient utilization of P. amurense.
基金supported by the 12th five-year National Science and Technology plan of China(2012BAC01B03)the 111 Project of China(B13007)
文摘Phellodendron amurense has been used for many years as a medical plant in traditional Chinese medicine and has shown great prospect in recent clinical trials for future applications. Berberine is an essential active compound contained in P. amurense. Our objective in this study was to quantify the content of berberine in P.amurense from sites at different elevations on Changbai Mountain. We collected samples of P. amurense from five different elevations on Changbai Mountain. Berberine in samples was extracted by ultrahigh pressure extraction(UPE). And the quantity was measured by high performance liquid chromatography(HPLC). First, the optimal HPLC conditions for berberine were identified with satisfactory precision(relative standard deviation, R SD\5.6 %), good accuracy(relative error, R E\ 3.6 %) and good linear relation(R2= 0.9998) in the range of 6.576–328.8 mg L-1. Second, the combination of UPE and HPLC methods in quantitative analysis of berberine showed high repeatability(R SD= 3.28 %), reproducibility(R SD= 4.72 %),stability(R SD\ 1.27 %) and good recovery(99.54 %) for real plant materials. Samples from Heilongjiang Province at the lowest elevation contained the highest amount of berberine.Similarly, the lowest amount of berberine was recorded in samples from Changbai Forest Bureau of Jilin Province collected at the highest elevation in this paper. The proposed UPE–HPLC method is simple, reliable and low-cost for quantitative analysis of berberine. Content of berberine in P.amurense varied significantly by site on Changbai Mountain.
基金Supported by Youth and Guidance Fund of Liaoning Institute of Science and Technology(Yd201605)
文摘[Objectives] This study was conducted to study the anti-gout effect of Phellodendron amurense and its different processed products. [Methods] Extracts of P. amurense and its different processed products were fed to rats respectively,to observe uric acid and creatinine in the serum and swelling index. [Results] Compared with the data of positive control,P. amurense and the processed products could significantly reduce the high uric acid and creatinine in the serum,and also could resist the swelling in rats. [Conclusions] P. amurense and its processed products showed different degrees of anti-gout effect,and the processing methods were found to have no remarkable impact on this effect.
