OBJECTIVE:To investigate the in vitro and in vivo studies of natural compounds and medicinal plants with anti-coronavirus activity.METHODS:A systematic review was performed based on Preferred Reporting Items for Syste...OBJECTIVE:To investigate the in vitro and in vivo studies of natural compounds and medicinal plants with anti-coronavirus activity.METHODS:A systematic review was performed based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses and Animal Research:Reporting of in vivo experiments guidelines to find data for medicinal plants and natural products effective against human coronaviruses in in vitro or in vivo studies.Studies published up to September 6,2020 were included.Studies(in vitro or in vivo)reporting the effect of medicinal plants and natural products or their derivatives on human coronavirus were included RESULTS:Promising anti-coronavirus effects are seen with different herbal compounds like some diterpenoids,sesquiterpenoids,and three compounds in tea with 3 CLpro inhibiting effect of Severe Acute Respiratory Syndrome Coronavirus(SARS-Co V);Hirsutenone,Six cinnamic amides and bavachinin are PLpro inhibitors and Tanshinones are active on both 3 CLpro and PLpro.Some flavonoid compounds of Citrus fruits act on Immunoregulation and target angiotensin-converting enzyme 2 which is used by SARS-COV for entry.Virus helicase is possibly inhibited by two compounds myricetin and scutellarein.CONCLUSION:This review shows that complementary medicine have the potential for new drug discovery against coronavirus.Further research is needed before definitive conclusions can be made concerning the safety and efficacy of the use of these medicinal plants.展开更多
Objective: The roots of two Thai medicinal plants, Clerodendrum indicum and Clerodendrum villosum are found in traditional medicine practices. The aim of this research was to preliminarily study the cytotoxicity of ex...Objective: The roots of two Thai medicinal plants, Clerodendrum indicum and Clerodendrum villosum are found in traditional medicine practices. The aim of this research was to preliminarily study the cytotoxicity of extracts of their roots, and the parts that possessed cytotoxic activity were separated on a chromatograph to identify their active compounds.Methods: The extracts of both plants were screened for cytotoxicity on the SW620 cell line and the compounds isolated from the active extracts were further evaluated for their cytotoxic activity against five human cancer cell lines, including SW620, Cha Go-K-1, Hep G2, KATO-III and BT-474 using the 3-(4,5-dime thyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay.Results: Dichloromethane extracts of C. indicum and C. villosum were active against the SW620 cell line.Triterpenoids were mostly obtained from the extracts of these plants(0.28% and 1.02%, respectively) and exhibited varying degrees of cytotoxicity and specificity against the tested cell lines. Two triterpenoids,oleanolic acid 3-acetate and betulinic acid, displayed moderate to strong cytotoxicity toward all cancer cell lines, with 50% inhibitory concentration(IC50) values of 1.66–20.49 mmol/L, whereas 3 b-hydroxyD:B-friedo-olean-5-ene and taraxerol were cytotoxic to only the SW620 cell line(IC50= 23.39 and2.09 mmol/L, respectively). Triterpenoid, lupeol, showed potent cytotoxicity on both SW620(IC50=1.99 mmol/L) and KATO-III cell lines(IC50= 1.95 mmol/L), while a flavonoid, pectolinarigenin, displayed moderate cytotoxicity against these cells(IC50= 13.05 and 24.31 mmol/L, respectively). Although the widely distributed steroid, stigmasterol, was effective against the SW620 cell line(IC50= 2.79 mmol/L)and b-sitosterol was also active against SW620(IC50= 11.26 mmol/L), BT-474(IC50= 14.11 mmol/L) and Hep G2 cancer cells(IC50= 20.47 mmol/L), none of the characteristic 24 b-ethylsteroids of either Clerodendrum species were shown to be cytotoxic.Conclusion: This study is the first report on the presence of cytotoxic triterpenoids from the roots of these medicinal plants, which have been used in herbal formulas as an antipyretic. Our findings support further in-depth study of this pharmacological activity as an anticancer agent.展开更多
OBJECTIVE:To investigate the mechanism underlying the effects of Shichangpu(Rhizoma Acori Tatarinowii)on attention deficit hyperactivity disorder(ADHD).METHODS:A network pharmacology approach integrating ingredients o...OBJECTIVE:To investigate the mechanism underlying the effects of Shichangpu(Rhizoma Acori Tatarinowii)on attention deficit hyperactivity disorder(ADHD).METHODS:A network pharmacology approach integrating ingredients of Shichangpu(Rhizoma Acori Tatarinowii)and target with ADHD,network construction,molecular function interactions and pathway analysis was used.RESULTS:This approach successfully helped to identify 7 active ingredients of CN,interacting with21 key targets(ADRA1 A,ADRA1 B,ADRA2 A,ADRA2 B,ADRA2 C,ADRB1,ADRB2,CHRM1,CHRM2,CHRM3,PTGS1,SLC6 A2,SLC6 A3,SLC6 A4,DRD1,DRD5,HTR2 A,ADRA1 D,MAOB,GRIA2,HTR1 A).The molecular function interactions among candidate targets mainly consisted of four groups:G-protein coupled amine receptor activity,catecholamine binding,monoamine transmembrane transporter activity and neurotransmitter receptor activity.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that Shichangpu(Rhizoma Acori Tatarinowii)-regulated pathways were mainly classified into signal transduction and monoamine neurotransmitters.CONCLUSION:Our investigation revealed that Shichangpu(Rhizoma Acori Tatarinowii)could improve the symptoms of ADHD by regulating neurotransmitter,in multiple types of compounds-target-pathway,which may be implicated in the major pathological processes of ADHD.展开更多
文摘OBJECTIVE:To investigate the in vitro and in vivo studies of natural compounds and medicinal plants with anti-coronavirus activity.METHODS:A systematic review was performed based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses and Animal Research:Reporting of in vivo experiments guidelines to find data for medicinal plants and natural products effective against human coronaviruses in in vitro or in vivo studies.Studies published up to September 6,2020 were included.Studies(in vitro or in vivo)reporting the effect of medicinal plants and natural products or their derivatives on human coronavirus were included RESULTS:Promising anti-coronavirus effects are seen with different herbal compounds like some diterpenoids,sesquiterpenoids,and three compounds in tea with 3 CLpro inhibiting effect of Severe Acute Respiratory Syndrome Coronavirus(SARS-Co V);Hirsutenone,Six cinnamic amides and bavachinin are PLpro inhibitors and Tanshinones are active on both 3 CLpro and PLpro.Some flavonoid compounds of Citrus fruits act on Immunoregulation and target angiotensin-converting enzyme 2 which is used by SARS-COV for entry.Virus helicase is possibly inhibited by two compounds myricetin and scutellarein.CONCLUSION:This review shows that complementary medicine have the potential for new drug discovery against coronavirus.Further research is needed before definitive conclusions can be made concerning the safety and efficacy of the use of these medicinal plants.
基金supported by a grant from Research Institute of Rangsit University (No. 40/2556)
文摘Objective: The roots of two Thai medicinal plants, Clerodendrum indicum and Clerodendrum villosum are found in traditional medicine practices. The aim of this research was to preliminarily study the cytotoxicity of extracts of their roots, and the parts that possessed cytotoxic activity were separated on a chromatograph to identify their active compounds.Methods: The extracts of both plants were screened for cytotoxicity on the SW620 cell line and the compounds isolated from the active extracts were further evaluated for their cytotoxic activity against five human cancer cell lines, including SW620, Cha Go-K-1, Hep G2, KATO-III and BT-474 using the 3-(4,5-dime thyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay.Results: Dichloromethane extracts of C. indicum and C. villosum were active against the SW620 cell line.Triterpenoids were mostly obtained from the extracts of these plants(0.28% and 1.02%, respectively) and exhibited varying degrees of cytotoxicity and specificity against the tested cell lines. Two triterpenoids,oleanolic acid 3-acetate and betulinic acid, displayed moderate to strong cytotoxicity toward all cancer cell lines, with 50% inhibitory concentration(IC50) values of 1.66–20.49 mmol/L, whereas 3 b-hydroxyD:B-friedo-olean-5-ene and taraxerol were cytotoxic to only the SW620 cell line(IC50= 23.39 and2.09 mmol/L, respectively). Triterpenoid, lupeol, showed potent cytotoxicity on both SW620(IC50=1.99 mmol/L) and KATO-III cell lines(IC50= 1.95 mmol/L), while a flavonoid, pectolinarigenin, displayed moderate cytotoxicity against these cells(IC50= 13.05 and 24.31 mmol/L, respectively). Although the widely distributed steroid, stigmasterol, was effective against the SW620 cell line(IC50= 2.79 mmol/L)and b-sitosterol was also active against SW620(IC50= 11.26 mmol/L), BT-474(IC50= 14.11 mmol/L) and Hep G2 cancer cells(IC50= 20.47 mmol/L), none of the characteristic 24 b-ethylsteroids of either Clerodendrum species were shown to be cytotoxic.Conclusion: This study is the first report on the presence of cytotoxic triterpenoids from the roots of these medicinal plants, which have been used in herbal formulas as an antipyretic. Our findings support further in-depth study of this pharmacological activity as an anticancer agent.
基金Supported by the National Natural Science Foundation of China(No.81774366)National Major New Drug Creation Project(No.2014ZX09304-306-002).
文摘OBJECTIVE:To investigate the mechanism underlying the effects of Shichangpu(Rhizoma Acori Tatarinowii)on attention deficit hyperactivity disorder(ADHD).METHODS:A network pharmacology approach integrating ingredients of Shichangpu(Rhizoma Acori Tatarinowii)and target with ADHD,network construction,molecular function interactions and pathway analysis was used.RESULTS:This approach successfully helped to identify 7 active ingredients of CN,interacting with21 key targets(ADRA1 A,ADRA1 B,ADRA2 A,ADRA2 B,ADRA2 C,ADRB1,ADRB2,CHRM1,CHRM2,CHRM3,PTGS1,SLC6 A2,SLC6 A3,SLC6 A4,DRD1,DRD5,HTR2 A,ADRA1 D,MAOB,GRIA2,HTR1 A).The molecular function interactions among candidate targets mainly consisted of four groups:G-protein coupled amine receptor activity,catecholamine binding,monoamine transmembrane transporter activity and neurotransmitter receptor activity.Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that Shichangpu(Rhizoma Acori Tatarinowii)-regulated pathways were mainly classified into signal transduction and monoamine neurotransmitters.CONCLUSION:Our investigation revealed that Shichangpu(Rhizoma Acori Tatarinowii)could improve the symptoms of ADHD by regulating neurotransmitter,in multiple types of compounds-target-pathway,which may be implicated in the major pathological processes of ADHD.
