Atmospheric pressure cold plasma, with advantages such as high particle activity, no thermal damage, high efficiency and direct and friendly contact with human tissues, is considered to have great potential in biomedi...Atmospheric pressure cold plasma, with advantages such as high particle activity, no thermal damage, high efficiency and direct and friendly contact with human tissues, is considered to have great potential in biomedical applications. Therefore, 'plasma medicine' as a new interdiscipline has been developed in the past two decades. This review first briefly describes the development of typical plasma sources suitable for biomedical applications, and those with different discharge forms are simply compared, evaluated and summarized. Subsequently, measurement of the crucial gaseous reactive particles(e.g. OH and O) and their spatio-temporal distributions are introduced. Meanwhile, the generation and variation rules and the related critical macroscopic parameters of the plasma-induced aqueous reactive species are summarized. Finally, related studies in the last ten years on the mechanisms of the plasma-driven microbial inactivation and plasma-induced apoptosis of cancer cells are introduced. Moreover, some scientific problems that need to be urgently solved in the field of plasma medicine are also discussed. This review will provide useful guidance for future related research.展开更多
Renal cell carcinoma (RCC) is one of the most important urological tumors and is one of the most common cancer diseases worldwide. Unfortunately, the treatment options are very limited due to resistances. Non-invasive...Renal cell carcinoma (RCC) is one of the most important urological tumors and is one of the most common cancer diseases worldwide. Unfortunately, the treatment options are very limited due to resistances. Non-invasive physical plasma (NIPP) is currently becoming a promising and very well tolerated treatment option for cancer. NIPP represents a highly energized gas and induc</span><span style="font-family:Verdana;">es varying antioncogenic cell responses in tumor cells. And also in t</span><span style="font-family:Verdana;">he case of RCC, NIPP treatment has great potential to enhance and supplement existing anticancer treatment options. Outstanding characteristics of NIPP treatment are 1) a precise and local effect on the treated tissue and 2) an almost exclusive effect on treated tumor cells without side effects. This allows </span><span style="font-family:Verdana;">an enormously large therapeutic window and makes the combination o</span><span style="font-family:Verdana;">f NIPP treatment and classical therapy appear particularly promising. In addition to R</span><span style="font-family:Verdana;">CC, plasma oncology offers an extremely innovative physical treatme</span><span style="font-family:Verdana;">nt method for future oncology in general.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">This brief review article summarizes the current knowledge on the potential use of NIPP in RCC therapy.展开更多
In this study two plasma sources were used for an in vivo treatment of human stratum corneum. The sample preparation was realised with the Cyanoacrylat stripping method, whereby a few layers of corneocytes embedded in...In this study two plasma sources were used for an in vivo treatment of human stratum corneum. The sample preparation was realised with the Cyanoacrylat stripping method, whereby a few layers of corneocytes embedded in the lipid matrix were removed from the skin of healthy volunteers. For the plasma treatment, dielectric barrier discharges with pulse durations in the microsecond as well as in the nanosecond range were applied. A comparison of these sources with respect to their biologically active components including dissipated power, gas and electron temperature, irradiance in the ultraviolet range, ozone and nitric oxide concentration is presented. Furthermore, species generated during plasma treatment on the sample surface like hydrogen peroxide, nitride or nitrate were measured using reflectometry. In addition, safety aspects for both sources were evaluated. Resulting plasma induced changes in the sample composition were investigated through X-ray photoelectron spectroscopy. The main ingredients carbon, oxygen, and nitrogen in addition to minor concentrations of sulphur were considered. A significant influence of the pulse duration on plasma characteristics was shown. A more effective formation of reactive species as well as more intense UV emission for ns-plasma was observed. Based on the determined parameters, both plasma sources are suitable for therapeutic purpose. Furthermore, significant plasma induced changes in the stratum corneum composition were reported, including an increase in nitrogen and oxygen content.展开更多
In this work, the suitability of lipid stripping as an alternative model of stratum corneum for plasma medical studies was investigated. Plasma treatment experiments were performed on samples prepared by the cyanoacry...In this work, the suitability of lipid stripping as an alternative model of stratum corneum for plasma medical studies was investigated. Plasma treatment experiments were performed on samples prepared by the cyanoacrylat stripping method. Therefore, two different dielectric barrier discharge-based plasma sources driven by high-voltage pulses in the microsecond and nanosecond range were applied. The lipid sample heating, change in pH-value, and the interaction with plasma-induced UV-radiation are presented and discussed with respect to existing findings on skin samples. After the plasma treatment, the lipid stripping shows similar changes compared to human skin relating to sample heating and pH-value. The investigation of the interplay with UV- radiation shows a high absorption in the wavelength range of 250 nm up to 400 nm. Further, the thickness, surface structure, and composition of lipid stripping samples were determined. The stripped sample shows a thickness of 3 ± 1 μm whereby approximately 30% of the sample surface is covered by lipids. In addition, it was shown that there are no changes in structure caused by the sample preparation. Based on the results of this work, it can be stated that lipid stripping represents an appropriate skin model for plasma medical investigations.展开更多
基金financially supported by National Natural Science Foundation of China (Grant Nos. 51777206, 51807046 and 51877208)the Natural Science Foundation of Anhui Province (Grant Nos. 1908085MA29 and 1808085MA13)。
文摘Atmospheric pressure cold plasma, with advantages such as high particle activity, no thermal damage, high efficiency and direct and friendly contact with human tissues, is considered to have great potential in biomedical applications. Therefore, 'plasma medicine' as a new interdiscipline has been developed in the past two decades. This review first briefly describes the development of typical plasma sources suitable for biomedical applications, and those with different discharge forms are simply compared, evaluated and summarized. Subsequently, measurement of the crucial gaseous reactive particles(e.g. OH and O) and their spatio-temporal distributions are introduced. Meanwhile, the generation and variation rules and the related critical macroscopic parameters of the plasma-induced aqueous reactive species are summarized. Finally, related studies in the last ten years on the mechanisms of the plasma-driven microbial inactivation and plasma-induced apoptosis of cancer cells are introduced. Moreover, some scientific problems that need to be urgently solved in the field of plasma medicine are also discussed. This review will provide useful guidance for future related research.
文摘Renal cell carcinoma (RCC) is one of the most important urological tumors and is one of the most common cancer diseases worldwide. Unfortunately, the treatment options are very limited due to resistances. Non-invasive physical plasma (NIPP) is currently becoming a promising and very well tolerated treatment option for cancer. NIPP represents a highly energized gas and induc</span><span style="font-family:Verdana;">es varying antioncogenic cell responses in tumor cells. And also in t</span><span style="font-family:Verdana;">he case of RCC, NIPP treatment has great potential to enhance and supplement existing anticancer treatment options. Outstanding characteristics of NIPP treatment are 1) a precise and local effect on the treated tissue and 2) an almost exclusive effect on treated tumor cells without side effects. This allows </span><span style="font-family:Verdana;">an enormously large therapeutic window and makes the combination o</span><span style="font-family:Verdana;">f NIPP treatment and classical therapy appear particularly promising. In addition to R</span><span style="font-family:Verdana;">CC, plasma oncology offers an extremely innovative physical treatme</span><span style="font-family:Verdana;">nt method for future oncology in general.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">This brief review article summarizes the current knowledge on the potential use of NIPP in RCC therapy.
文摘In this study two plasma sources were used for an in vivo treatment of human stratum corneum. The sample preparation was realised with the Cyanoacrylat stripping method, whereby a few layers of corneocytes embedded in the lipid matrix were removed from the skin of healthy volunteers. For the plasma treatment, dielectric barrier discharges with pulse durations in the microsecond as well as in the nanosecond range were applied. A comparison of these sources with respect to their biologically active components including dissipated power, gas and electron temperature, irradiance in the ultraviolet range, ozone and nitric oxide concentration is presented. Furthermore, species generated during plasma treatment on the sample surface like hydrogen peroxide, nitride or nitrate were measured using reflectometry. In addition, safety aspects for both sources were evaluated. Resulting plasma induced changes in the sample composition were investigated through X-ray photoelectron spectroscopy. The main ingredients carbon, oxygen, and nitrogen in addition to minor concentrations of sulphur were considered. A significant influence of the pulse duration on plasma characteristics was shown. A more effective formation of reactive species as well as more intense UV emission for ns-plasma was observed. Based on the determined parameters, both plasma sources are suitable for therapeutic purpose. Furthermore, significant plasma induced changes in the stratum corneum composition were reported, including an increase in nitrogen and oxygen content.
基金supported by the European Regional Development Funds(EFRE)and the Workgroup Innovative Projects of Lower Saxony(AGiP)in the frame of the Lower Saxony Innovation Network for Plasma Technology(NIP),project funding reference number W2-80029388.
文摘In this work, the suitability of lipid stripping as an alternative model of stratum corneum for plasma medical studies was investigated. Plasma treatment experiments were performed on samples prepared by the cyanoacrylat stripping method. Therefore, two different dielectric barrier discharge-based plasma sources driven by high-voltage pulses in the microsecond and nanosecond range were applied. The lipid sample heating, change in pH-value, and the interaction with plasma-induced UV-radiation are presented and discussed with respect to existing findings on skin samples. After the plasma treatment, the lipid stripping shows similar changes compared to human skin relating to sample heating and pH-value. The investigation of the interplay with UV- radiation shows a high absorption in the wavelength range of 250 nm up to 400 nm. Further, the thickness, surface structure, and composition of lipid stripping samples were determined. The stripped sample shows a thickness of 3 ± 1 μm whereby approximately 30% of the sample surface is covered by lipids. In addition, it was shown that there are no changes in structure caused by the sample preparation. Based on the results of this work, it can be stated that lipid stripping represents an appropriate skin model for plasma medical investigations.
