The antioxidant property of Moringa oleifera leaves and Pleurotus ostreatus on swimming performance and related biochemical parameters were investigated in thirty adult male and female wistar rats. Purpose: The aim of...The antioxidant property of Moringa oleifera leaves and Pleurotus ostreatus on swimming performance and related biochemical parameters were investigated in thirty adult male and female wistar rats. Purpose: The aim of the present study was to evaluate the antioxidant property of M. oleifera and P. ostreatus mixture in specific proportions in wistar rats. Material and Methods: The mushroom species and M. oleifera leaves were cultivated at the biotechnology laboratory of the University Cheikh Anta Diop de Dakar, Senegal. Oxidative stress in rats was induced by swimming. The dietary supplement was composed of a mixture of powders of Moringa oleifera and Pleurotus ostreatus in 2:1 ratio. Three groups received different doses of dietary supplements 500 mg/kg, 1000 mg/kg, and 1500 mg/kg, with one group being the stressed and the other the control group. Parameters in serum such as serum glutamyl oxaloacetate transaminase, serum glutamyl pyruvate transaminase, albumin, Testosterone, creatinine, and, oxidative stress parameters (catalase, reduced glutathione, and malondialdehyde) were measured. Results: Results showed that the dietary supplement has an effect on oxidative stress because it increases the activity of catalase and the level of cellular glutathione in rats. The 500 mg/kg dose would be the most appropriate dose for stressful conditions. The 1000 mg/kg dose would be the most appropriate dose for liver damage. Conclusion: This study shows that the antioxidant properties of M. oleifera leaves and Pleurotus ostreatus are demonstrated by their ability to improve body energy stores and tissue antioxidant capacity. The dietary supplement of M. oleifera leaves and P. ostreatus powders mixture could be good in stressful conditions.展开更多
<em>Pleurotus ostreatus </em>was obtained from the wild and evaluated for its antibacterial and antioxidant activities. The antibacterial activities of the methanolic and aqueous extracts against clinical ...<em>Pleurotus ostreatus </em>was obtained from the wild and evaluated for its antibacterial and antioxidant activities. The antibacterial activities of the methanolic and aqueous extracts against clinical isolates of <em>Klebsiella pneumoniae</em>, <em>Staphylococcus aureus</em>, <em>Pseudomonas aeruginosa</em> and <em>Escherichia coli</em> with vancomycine and amicosin as positive control against the isolates were examined using agar well diffusion assay and the zones of inhibition for the methanolic extract varied for different organisms as zones of inhibition were highly pronounced in Escherichia coli having 18.0 ± 1.41 mm followed by <em>Klebsiella pneumoniae</em> of 17.0 ± 0.58 mm then <em>Staphylococcus aureus</em> of 14.0 ± 1.53 mm and finally <em>Pseudomonas aeruginosa</em> 13.0 ± 0.58 mm. While for the aqueous extract, no inhibition was observed in three isolates apart from <em>Pseudomonas aeruginosa</em> in which the zone of inhibition was just 8.0 ± 1.00 mm. The total antioxidant activity of aqueous <em>Pleurotus ostreatus </em>differed significantly with total phenolic of 12.55 ± 0.11 mg GAE/g, total flavonoid of 7.22 ± 1.60 mg QE/g, ABTS of 1.99 ± 0.06 mmol TEAC/100g, FRAP of mean value of 5.25 ± 0.25 mg/g and also <em>Pleurotus ostreatus</em> was able to scavenged DPPH in a dose-dependent manner of 0 - 13.3 mg/ml. The inhibitory effect of <em>Pleurotus ostreatus</em> can therefore be attributed to the presence of phenolic and flavonoid compounds in the extract. Also, the result of the ABTS+ scavenging ability of <em>Pleurotus ostreatus</em> as trolox equivalent antioxidant capacity (TEAC) revealed that the extracts of <em>Pleurotus ostreatus</em> has ABTS scavenging ability. The FRAP mean value shows the ability of <em>Pleurotus ostreatus</em> extract to reduced Iron (III) to Iron (II) indicates its redox potential which presents the mushroom a good antioxidant source, suitable for health benefits when consumed.展开更多
The effect of propolis, taken from honeybee hives in Tokat (Turkey), on mycelium development of oyster mushroom (Pleurotus ostreatus) was investigated. Propolis contains various chemical com- pounds and has antiviral,...The effect of propolis, taken from honeybee hives in Tokat (Turkey), on mycelium development of oyster mushroom (Pleurotus ostreatus) was investigated. Propolis contains various chemical com- pounds and has antiviral, antibacterial and antifungal activities. Mushroom spores were inoculated in Potato Dextrose Agar (PDA) media containing different proportions of propolis. Mycelium development on media containing 0.10%, 0.30%, and 0.50% propolis occured at the first, third, and fifth days, respectively. The fastest and most intense development was seen on medium containing 0.10% propolis. According to our results, propolis can be used as an enriching material in mushroom cultivating.展开更多
Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the indu...Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the industrially important manganese peroxidase (MnP) enzyme was recorded after five days of still culture incubation. The crude MnP was 2.1-fold purified with a specific activity of 860 U/mg after purification on a Sephadex-G-100 gel column. On native and SDS-PAGE electrophoresis gels, the purified MnP fraction was a single homogenous band of 45 kDa. An active fraction of MnP was immobilized using hydrophobic sol-gel entrapment comprising tetramethoxysilane (T) and propyltrimethoxysilane (P) at different T:P molar ratios. Characterization revealed that after 24 h incubation at varying pH and temperatures, the MnP fraction immobilized at a T:P ratio of 1:2 in the sol-gel retained 82% and 75% of its original activity at pH4 and 70 ℃, respectively. The optimally active fraction at a 1:2 T:P ratio was tested against MnSO4 as a substrate to determine the kinetic catalytic constants KM and Vmax . To explore the industrial applicability of P. ostreatus IBL-02 MnP, both the free and immobilized MnP were used for the decolorization of four different textile industrial effluents. A maximum of 100% decolorization was achieved for the different textile effluents within the shortest time period. A lower KM , higher Vmax , hyper-activation, and enhanced acidic and thermal resistance up to 70 ℃ were the novel catalytic features of the sol-gel immobilized MnP, suggesting that it may be a potential candidate for biotechnological applications particularly for textile bioremediation purposes.展开更多
[Objectives] This study was conducted to investigate the effects of different proportions of spent Pleurotus ostreatus substrate on the germination and seedling growth of mung beans. [Methods] The cellulose-degrading ...[Objectives] This study was conducted to investigate the effects of different proportions of spent Pleurotus ostreatus substrate on the germination and seedling growth of mung beans. [Methods] The cellulose-degrading bacteria HB8 and HF1 were mixed with a commercially available microbial composting agent, respectively, for the composting of spent P. ostreatus substrate. Mung beans were cultivated with different proportions of spent mushroom substrate compost and soil. The seed germination rate, plant height, fresh weight and chlorophyll content of mung bean were used as indicators to investigate the effects of the organic fertilizer from spent P. ostreatus substrate on the growth of mung bean seedlings. [Results] The addition of cellulose-degrading bacteria can significantly improve the composting effect of the spent mushroom substrate. After 8 d of cultivation of mung beans with different ratios of the mushroom substrate organic fertilizer, 50% of the organic fertilizer can make the plant height, fresh weight and leaf chlorophyll content of mung bean seedlings reach the highest value and was suitable for mung bean breeding and cultivation. [Conclusions] This study provides scientific basis and technical indicators for the rapid and harmless treatment of spent mushroom substrate and its application in crop cultivation and nursery.展开更多
In the present study,agar diffusion test and field trials were carried out using a mushroom(Pleurotus ostreatus)cultivar Shouyanping as the experimental material to determine the antibacterial activity of refined pyro...In the present study,agar diffusion test and field trials were carried out using a mushroom(Pleurotus ostreatus)cultivar Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasii and its effects on mycelial growth of mushroom.The results revealed that 1.25%,2.50%and 5.00%of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom,but also greatly promoted mushroom mycelial growth.Therefore,1.25%and 2.50%of refined pyroligneous acid can be used in fields to prevent brown blotch disease,and 5.00%refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.展开更多
Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal speci...Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal species to degrade conventional and degradable plastic films. Plastics studied were oxo-degradable polyethylene (OXO-PE), UV-irradiated oxo-degradable polyethylene (UV-OXO-PE), polylactic acid (PLA) and conventional polyethylene (C-PE). The cultivation of P. ostreatus resulted in a reduction in the median of weight (78.2% - 80.2%) and volume (56.1% - 60.1%) of the substrate (wheat straw). Degradation of the plastics embodied was evidenced by a reduction in the median of the elongation at break (OXO-PE 475% to 109%, UV-OXO-PE 23% to 8%, PLA 596% to 398% and C-PE 505% to 304%) and an increase in the median of the carbonyl index (OXO-PE 0.062 to 0.114, UV-OXO-PE 0.098 to 0.145 and PLA 0.024 to 0.034). The Kruskal-Wallis test found no statistical difference (p = 0.384) between the medians of the biological efficiency for substrates containing plastics and the substrate without plastic. In conclusion, plastics embodied in the substrates used for cultivation of P. ostreatus are degraded and the degradation of these plastics does not affect the short term growth of P. ostreatus.展开更多
The control effects and application methods of Pleurotus ostreatus on the peanut root-knot nematode disease, Meloidogyne arenaria, were systematically studied in the greenhouse. Results of pot experiments showed that ...The control effects and application methods of Pleurotus ostreatus on the peanut root-knot nematode disease, Meloidogyne arenaria, were systematically studied in the greenhouse. Results of pot experiments showed that P. Ostreatus could effectively reduce the 2nd-instar population density in the soil, and the larva reduced by 80% compared with the control. P. Ostreatus could markedly lower the infecting number of the peanut root-knot nematodes, the nematode density inside the root knots declined by 40.00% - 82.64%, and the peanut root knots also reduced by 86.96% - 94.03%. The fungus could defer the initial infection of M. Arenaria to the roots, the epidemic rate of the peanut root-knot nematode disease was significantly cut down, and the increase of root-knot changed from the way of Logistic curve in the control to the way of linear curve in the treatment. The final control effectiveness was about 80%. The results of pot trial also indicated that the control effectiveness is closely related to the application time and the amount of this fungus. The key factor affecting control effectiveness was the application time of P. Ostreatus in the soil and the suitable time was 20 days before sowing.展开更多
[Objective] The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose.[Method]0.5 mmol/L Fe SO4,Cu SO4,Mn SO4 and Zn SO4 solutions were added to the bas...[Objective] The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose.[Method]0.5 mmol/L Fe SO4,Cu SO4,Mn SO4 and Zn SO4 solutions were added to the basal culture media containing silkworm excrement and rice husk,and the effects of metal ions on activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii were analyzed. [Result]The addition of Zn2 +and Mn2 +to the basal culture media containing silkworm excrement and rice husk was beneficial to the improvement of the activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii,while the addition of Fe2 +and Cu2 +inhibited the activity of the cellulase. [Conclusion ] The research could provide theoretical references for improvement of utilization rate of cellulase in silkworm excrement and rice husk.展开更多
文摘The antioxidant property of Moringa oleifera leaves and Pleurotus ostreatus on swimming performance and related biochemical parameters were investigated in thirty adult male and female wistar rats. Purpose: The aim of the present study was to evaluate the antioxidant property of M. oleifera and P. ostreatus mixture in specific proportions in wistar rats. Material and Methods: The mushroom species and M. oleifera leaves were cultivated at the biotechnology laboratory of the University Cheikh Anta Diop de Dakar, Senegal. Oxidative stress in rats was induced by swimming. The dietary supplement was composed of a mixture of powders of Moringa oleifera and Pleurotus ostreatus in 2:1 ratio. Three groups received different doses of dietary supplements 500 mg/kg, 1000 mg/kg, and 1500 mg/kg, with one group being the stressed and the other the control group. Parameters in serum such as serum glutamyl oxaloacetate transaminase, serum glutamyl pyruvate transaminase, albumin, Testosterone, creatinine, and, oxidative stress parameters (catalase, reduced glutathione, and malondialdehyde) were measured. Results: Results showed that the dietary supplement has an effect on oxidative stress because it increases the activity of catalase and the level of cellular glutathione in rats. The 500 mg/kg dose would be the most appropriate dose for stressful conditions. The 1000 mg/kg dose would be the most appropriate dose for liver damage. Conclusion: This study shows that the antioxidant properties of M. oleifera leaves and Pleurotus ostreatus are demonstrated by their ability to improve body energy stores and tissue antioxidant capacity. The dietary supplement of M. oleifera leaves and P. ostreatus powders mixture could be good in stressful conditions.
文摘<em>Pleurotus ostreatus </em>was obtained from the wild and evaluated for its antibacterial and antioxidant activities. The antibacterial activities of the methanolic and aqueous extracts against clinical isolates of <em>Klebsiella pneumoniae</em>, <em>Staphylococcus aureus</em>, <em>Pseudomonas aeruginosa</em> and <em>Escherichia coli</em> with vancomycine and amicosin as positive control against the isolates were examined using agar well diffusion assay and the zones of inhibition for the methanolic extract varied for different organisms as zones of inhibition were highly pronounced in Escherichia coli having 18.0 ± 1.41 mm followed by <em>Klebsiella pneumoniae</em> of 17.0 ± 0.58 mm then <em>Staphylococcus aureus</em> of 14.0 ± 1.53 mm and finally <em>Pseudomonas aeruginosa</em> 13.0 ± 0.58 mm. While for the aqueous extract, no inhibition was observed in three isolates apart from <em>Pseudomonas aeruginosa</em> in which the zone of inhibition was just 8.0 ± 1.00 mm. The total antioxidant activity of aqueous <em>Pleurotus ostreatus </em>differed significantly with total phenolic of 12.55 ± 0.11 mg GAE/g, total flavonoid of 7.22 ± 1.60 mg QE/g, ABTS of 1.99 ± 0.06 mmol TEAC/100g, FRAP of mean value of 5.25 ± 0.25 mg/g and also <em>Pleurotus ostreatus</em> was able to scavenged DPPH in a dose-dependent manner of 0 - 13.3 mg/ml. The inhibitory effect of <em>Pleurotus ostreatus</em> can therefore be attributed to the presence of phenolic and flavonoid compounds in the extract. Also, the result of the ABTS+ scavenging ability of <em>Pleurotus ostreatus</em> as trolox equivalent antioxidant capacity (TEAC) revealed that the extracts of <em>Pleurotus ostreatus</em> has ABTS scavenging ability. The FRAP mean value shows the ability of <em>Pleurotus ostreatus</em> extract to reduced Iron (III) to Iron (II) indicates its redox potential which presents the mushroom a good antioxidant source, suitable for health benefits when consumed.
文摘The effect of propolis, taken from honeybee hives in Tokat (Turkey), on mycelium development of oyster mushroom (Pleurotus ostreatus) was investigated. Propolis contains various chemical com- pounds and has antiviral, antibacterial and antifungal activities. Mushroom spores were inoculated in Potato Dextrose Agar (PDA) media containing different proportions of propolis. Mycelium development on media containing 0.10%, 0.30%, and 0.50% propolis occured at the first, third, and fifth days, respectively. The fastest and most intense development was seen on medium containing 0.10% propolis. According to our results, propolis can be used as an enriching material in mushroom cultivating.
基金a part of a research project entitled “the development of immobilized ligninolytic enzymes for industrial applications” supported by Higher Education Commission (HEC), Islamabad, Pakistan
文摘Solid state bio-processing of wheat straw was carried out through an indigenous fungal strain Pleurotus ostreatus IBL-02 under pre-optimized fermentation conditions. The maximum activity, 692±12 U/mL, of the industrially important manganese peroxidase (MnP) enzyme was recorded after five days of still culture incubation. The crude MnP was 2.1-fold purified with a specific activity of 860 U/mg after purification on a Sephadex-G-100 gel column. On native and SDS-PAGE electrophoresis gels, the purified MnP fraction was a single homogenous band of 45 kDa. An active fraction of MnP was immobilized using hydrophobic sol-gel entrapment comprising tetramethoxysilane (T) and propyltrimethoxysilane (P) at different T:P molar ratios. Characterization revealed that after 24 h incubation at varying pH and temperatures, the MnP fraction immobilized at a T:P ratio of 1:2 in the sol-gel retained 82% and 75% of its original activity at pH4 and 70 ℃, respectively. The optimally active fraction at a 1:2 T:P ratio was tested against MnSO4 as a substrate to determine the kinetic catalytic constants KM and Vmax . To explore the industrial applicability of P. ostreatus IBL-02 MnP, both the free and immobilized MnP were used for the decolorization of four different textile industrial effluents. A maximum of 100% decolorization was achieved for the different textile effluents within the shortest time period. A lower KM , higher Vmax , hyper-activation, and enhanced acidic and thermal resistance up to 70 ℃ were the novel catalytic features of the sol-gel immobilized MnP, suggesting that it may be a potential candidate for biotechnological applications particularly for textile bioremediation purposes.
基金Supported by Scientific Research Fund of Hunan Provincial Education Department(15C0721)Hunan Provincial Innovation Platform Open Fund Project(16K047)Hunan Provincial Construct Program of the Key Discipline
文摘[Objectives] This study was conducted to investigate the effects of different proportions of spent Pleurotus ostreatus substrate on the germination and seedling growth of mung beans. [Methods] The cellulose-degrading bacteria HB8 and HF1 were mixed with a commercially available microbial composting agent, respectively, for the composting of spent P. ostreatus substrate. Mung beans were cultivated with different proportions of spent mushroom substrate compost and soil. The seed germination rate, plant height, fresh weight and chlorophyll content of mung bean were used as indicators to investigate the effects of the organic fertilizer from spent P. ostreatus substrate on the growth of mung bean seedlings. [Results] The addition of cellulose-degrading bacteria can significantly improve the composting effect of the spent mushroom substrate. After 8 d of cultivation of mung beans with different ratios of the mushroom substrate organic fertilizer, 50% of the organic fertilizer can make the plant height, fresh weight and leaf chlorophyll content of mung bean seedlings reach the highest value and was suitable for mung bean breeding and cultivation. [Conclusions] This study provides scientific basis and technical indicators for the rapid and harmless treatment of spent mushroom substrate and its application in crop cultivation and nursery.
基金Supported by the fund of Science and Technology Department of Hebei Province(13226511)the fund of Chengde Municipal Finance Bureau(CZ2014002,CZ2013007)the fund of Chengde Municipal Science and Technology Bureau(20157029)
文摘In the present study,agar diffusion test and field trials were carried out using a mushroom(Pleurotus ostreatus)cultivar Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasii and its effects on mycelial growth of mushroom.The results revealed that 1.25%,2.50%and 5.00%of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom,but also greatly promoted mushroom mycelial growth.Therefore,1.25%and 2.50%of refined pyroligneous acid can be used in fields to prevent brown blotch disease,and 5.00%refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.
文摘Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal species to degrade conventional and degradable plastic films. Plastics studied were oxo-degradable polyethylene (OXO-PE), UV-irradiated oxo-degradable polyethylene (UV-OXO-PE), polylactic acid (PLA) and conventional polyethylene (C-PE). The cultivation of P. ostreatus resulted in a reduction in the median of weight (78.2% - 80.2%) and volume (56.1% - 60.1%) of the substrate (wheat straw). Degradation of the plastics embodied was evidenced by a reduction in the median of the elongation at break (OXO-PE 475% to 109%, UV-OXO-PE 23% to 8%, PLA 596% to 398% and C-PE 505% to 304%) and an increase in the median of the carbonyl index (OXO-PE 0.062 to 0.114, UV-OXO-PE 0.098 to 0.145 and PLA 0.024 to 0.034). The Kruskal-Wallis test found no statistical difference (p = 0.384) between the medians of the biological efficiency for substrates containing plastics and the substrate without plastic. In conclusion, plastics embodied in the substrates used for cultivation of P. ostreatus are degraded and the degradation of these plastics does not affect the short term growth of P. ostreatus.
文摘The control effects and application methods of Pleurotus ostreatus on the peanut root-knot nematode disease, Meloidogyne arenaria, were systematically studied in the greenhouse. Results of pot experiments showed that P. Ostreatus could effectively reduce the 2nd-instar population density in the soil, and the larva reduced by 80% compared with the control. P. Ostreatus could markedly lower the infecting number of the peanut root-knot nematodes, the nematode density inside the root knots declined by 40.00% - 82.64%, and the peanut root knots also reduced by 86.96% - 94.03%. The fungus could defer the initial infection of M. Arenaria to the roots, the epidemic rate of the peanut root-knot nematode disease was significantly cut down, and the increase of root-knot changed from the way of Logistic curve in the control to the way of linear curve in the treatment. The final control effectiveness was about 80%. The results of pot trial also indicated that the control effectiveness is closely related to the application time and the amount of this fungus. The key factor affecting control effectiveness was the application time of P. Ostreatus in the soil and the suitable time was 20 days before sowing.
文摘[Objective] The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose.[Method]0.5 mmol/L Fe SO4,Cu SO4,Mn SO4 and Zn SO4 solutions were added to the basal culture media containing silkworm excrement and rice husk,and the effects of metal ions on activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii were analyzed. [Result]The addition of Zn2 +and Mn2 +to the basal culture media containing silkworm excrement and rice husk was beneficial to the improvement of the activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii,while the addition of Fe2 +and Cu2 +inhibited the activity of the cellulase. [Conclusion ] The research could provide theoretical references for improvement of utilization rate of cellulase in silkworm excrement and rice husk.
