Cationic liposome(Lipo) and polyethylenimine(PEI) are widely applied for nonviral gene transfection.In this study,in order to combine the favorable properties of Lipo and PEI systems for gene delivery,Lipo/PEI complex...Cationic liposome(Lipo) and polyethylenimine(PEI) are widely applied for nonviral gene transfection.In this study,in order to combine the favorable properties of Lipo and PEI systems for gene delivery,Lipo/PEI complexes with different contents of PEI(5%,10%,20% and 40% relative to phosphatidyl choline in reaction system) were prepared.The physicochemical properties of Lipo/PEI complexes,as well as the influences of PEI content on the storage stability,cytotoxicity and transfection efficiency were investigated.The transmission electron microscopy(TEM) images showed that Lipo/PEI complexes had smaller size compared to pure Lipo.The zeta potential values decreased with the increasing content of PEI.After storaged for 3 months at 4 ℃,obvious aggregation was observed when the addition of PEI content was up to 20%.In vitro cytotoxicity assay showed that Lipo/PEI complexes had decreased cytotoxicity over pure PEI,while the cytotoxicity was enhanced as the PEI content increased.Importantly,the luciferase activity assay and confocal microscope observation revealed that Lipo/PEI complexes prepared with the lowest amount of PEI(Lipo/PEI-5%)possessed the highest transfection efficiency.Thus,these results suggest that feeding the appropriate content of PEI in Lipo/PEI complexes allows them to be excellent vehicle for gene delivery.展开更多
The objective of this work is to study the influences of silica supports and PEG additive on the sorption performance of molecular basket sorbent(MBS) for CO_2 capture consisting of polyethylenimine and one of the fol...The objective of this work is to study the influences of silica supports and PEG additive on the sorption performance of molecular basket sorbent(MBS) for CO_2 capture consisting of polyethylenimine and one of the following supports: SBA-15(2-D structure), TUD-1(3-D sponge-like structure) and fumed silica HS-5(3-D disordered structure). Effects of the supports regarding pore structures and pore properties, the PEI loading amount as well as the sorption temperature were examined. Furthermore, polyethylene glycol(PEG) was introduced as an additive into the sorbents and its effect was investigated at different PEI loadings and sorption temperatures. The results suggest that the pore properties of MBS(after PEI loading) play a more important role in the CO_2 sorption capacity, rather than those of the supports alone.MBS with 3D pore structure exhibits higher CO_2 sorption capacity and amine efficiency than those with 2D-structured support. Among the sorbents studied, fumed silica(HS-5) based MBS showed the highest CO_2 sorption capacity in the temperature range of 30-95 °C, probably due to its unique interstitial pores formed by the aggregation of polymer-loaded SiO_2 particles. It was found that the temperature dependence is directly related to the PEI surface coverage layers. The more PEI surface coverage layers, the higher diffusion barrier for CO_2 and the stronger temperature dependence of CO_2 capacity. 3D MBS exceeds 2D MBS at the same PEI coverage layers due to lower diffusion barrier. Adding PEG can significantly enhance the CO_2 sorption capacity and improve amine efficiency of all MBS, most likely by alleviating the diffusion barrier within PEI bulk layers through the inter-molecular interaction between PEI and PEG.展开更多
A novel composite carrier of folic acid(FA)-polyethyleneimine(PEI)-ethosome(Eth)(FA-PEI-Eth)was developed for the treatment of cancers through loading and targeting delivery of multidrug(including gene and other drugs...A novel composite carrier of folic acid(FA)-polyethyleneimine(PEI)-ethosome(Eth)(FA-PEI-Eth)was developed for the treatment of cancers through loading and targeting delivery of multidrug(including gene and other drugs)into cancer cells.Physical and chemical property tests were done to prove the grafting of the composite.Gel retardation test was done to determine the optimal ratio of DNA@PEI complex,and cytocompatibility tests and tumor cell uptake tests were done to evaluate the efficiency of the composite.The results demonstrated that the FA-PEI-Eth could effectively deliver a gene and other drugs into tumor cells simultaneously,and suggested that this composite would be a promising carrier in tumor-targeted therapy applications.展开更多
In this study,two different methods were applied to disperse hollow silica nanoparticles(HSNP);one employed polyethylenimine(PEI)as the dispersant during the synthesis processes for preparing HSNP,while the other adde...In this study,two different methods were applied to disperse hollow silica nanoparticles(HSNP);one employed polyethylenimine(PEI)as the dispersant during the synthesis processes for preparing HSNP,while the other added PEI into suspensions of the prepared HSNP and used milling treatment to achieve the desired dispersion.It was found that adding PEI during the synthesis process of HSNP had no noticeable improvement in the dispersion,while adding PEI into suspen-sions of the prepared HSNP and utilizing milling treatment resulted in remarkable dispersion improvement.Therefore,the latter was chosen as the method in dispersing HSNP suspensions.The adsorption of PEI on the surface of HSNP and the stability of the aqueous suspensions was investigated.The results indicated that the adsorption of PEI on the surface of HSNP would increase the repulsive energy among particles,hence reducing the agglomeration of HSNP and improving the stability of the aqueous suspensions.The change of HSNP’s ■ potential after adding PEI and the rela-tionship between the adsorbed amount of PEI and pH were also investigated.展开更多
Semiconductor quantum dots(QDs)have shown great promise as fluorescent probes for molecular,cellular and in vivo imaging.However,the fluorescence of traditional polymer-encapsulated QDs is often quenched by proton-ind...Semiconductor quantum dots(QDs)have shown great promise as fluorescent probes for molecular,cellular and in vivo imaging.However,the fluorescence of traditional polymer-encapsulated QDs is often quenched by proton-induced etching in acidic environments.This is a major problem for applications of QDs in the gastrointestinal tract because the gastric(stomach)environment is strongly acidic(pH 12).Here we report the use of proton-resistant surface coatings to stabilize QD fl uorescence under acidic conditions.Using both hyperbranched polyethylenimine(PEI)and its polyethylene glycol derivative(PEG-grafted PEI),we show that the fl uorescence of coreshell CdSe/CdS/ZnS QDs is effectively protected from quenching in simulated gastric fluids.In comparison,amphiphilic lipid or polymer coatings provide no protection under similarly acidic conditions.The proton-resistant QDs are found to cause moderate membrane damage to cultured epithelial cells,but PEGylation(PEG grafting)can be used to reduce cellular toxicity and to improve nanoparticle stability.展开更多
Myocardial ischemia reperfusion(IR)injury is closely related to the overwhelming inflammation in the myocardium.Herein,cardiomyocyte-targeted nanotherapeutics were developed for the reactive oxygen species(ROS)-ultras...Myocardial ischemia reperfusion(IR)injury is closely related to the overwhelming inflammation in the myocardium.Herein,cardiomyocyte-targeted nanotherapeutics were developed for the reactive oxygen species(ROS)-ultrasensitive co-delivery of dexamethasone(Dex)and RAGE small interfering RNA(siRAGE)to attenuate myocardial inflammation.PPTP,a ROSdegradable polycation based on PGE2-modified,PEGylated,ditellurium-crosslinked polyethylenimine(PEI)was developed to surface-decorate the Dex-encapsulated mesoporous silica nanoparticles(MSNs),which simultaneously condensed siRAGE and gated the MSNs to prevent the Dex pre-leakage.Upon intravenous injection to IR-injured rats,the nanotherapeutics could be efficiently transported into the inflamed cardiomyocytes via PGE2-assisted recognition of over-expressed E-series of prostaglandin(EP)receptors on the cell membranes.Intracellularly,the over-produced ROS degraded PPTP into small segments,promoting the release of siRAGE and Dex to mediate effective RAGE silencing(72%)and cooperative antiinflammatory effect.As a consequence,the nanotherapeutics notably suppressed the myocardial fibrosis and apoptosis,ultimately recovering the systolic function.Therefore,the current nanotherapeutics represent an effective example for the codelivery and on-demand release of nucleic acid and chemodrug payloads,and might find promising utilities toward the synergistic management of myocardial inflammation.展开更多
The calcium phosphate (CaP) particles have attracted much attention in gene therapy. How to construct stable gene particles was the determining factor. In this study,hybrid multi-shell CaP gene particles were successf...The calcium phosphate (CaP) particles have attracted much attention in gene therapy. How to construct stable gene particles was the determining factor. In this study,hybrid multi-shell CaP gene particles were successfully constructed. First,CaP nanoparticles served as a core and were coated with DNA for colloidal stabilization. The ξ-potential of DNA-coated CaP nanoparticles was -15 mV. Then polyethylenimine (PEI) was added and adsorbed outside of the DNA layer due to the electrostatic attraction. The ξ-potential of hybrid multi-shell CaP particles was slightly positive. With addition of PEI,the hybrid multi-shell particles could condense DNA effectively,which was de-termined by ethidium bromide (EtBr) exclusion assay. The hybrid particles were spherical and uniform with diameters of about 150 nm at proper conditions. By simple modification of PEI,the hybrid multi-shell CaP gene particles were successfully constructed. They may have great potential in gene therapy.展开更多
基金National Natural Science Foundations of China(Nos.31271028,31570984)Innovation Program of Shanghai Municipal Education Commission,China(No.13ZZ051)+2 种基金International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality,China(No.15540723400)Open Foundation of State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,China(No.LK1416)“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)
文摘Cationic liposome(Lipo) and polyethylenimine(PEI) are widely applied for nonviral gene transfection.In this study,in order to combine the favorable properties of Lipo and PEI systems for gene delivery,Lipo/PEI complexes with different contents of PEI(5%,10%,20% and 40% relative to phosphatidyl choline in reaction system) were prepared.The physicochemical properties of Lipo/PEI complexes,as well as the influences of PEI content on the storage stability,cytotoxicity and transfection efficiency were investigated.The transmission electron microscopy(TEM) images showed that Lipo/PEI complexes had smaller size compared to pure Lipo.The zeta potential values decreased with the increasing content of PEI.After storaged for 3 months at 4 ℃,obvious aggregation was observed when the addition of PEI content was up to 20%.In vitro cytotoxicity assay showed that Lipo/PEI complexes had decreased cytotoxicity over pure PEI,while the cytotoxicity was enhanced as the PEI content increased.Importantly,the luciferase activity assay and confocal microscope observation revealed that Lipo/PEI complexes prepared with the lowest amount of PEI(Lipo/PEI-5%)possessed the highest transfection efficiency.Thus,these results suggest that feeding the appropriate content of PEI in Lipo/PEI complexes allows them to be excellent vehicle for gene delivery.
基金the support of this work at Penn State by the U.S.Department of Energy,National Energy Technology Laboratorythe financial support by the China Scholarship Council,the Natural Science Foundation of China(No.51176034)the Open Fund of Key Laboratory of Coal-Based CO2 Capture and Geological Storage of Jiangsu Province(2016A05)
文摘The objective of this work is to study the influences of silica supports and PEG additive on the sorption performance of molecular basket sorbent(MBS) for CO_2 capture consisting of polyethylenimine and one of the following supports: SBA-15(2-D structure), TUD-1(3-D sponge-like structure) and fumed silica HS-5(3-D disordered structure). Effects of the supports regarding pore structures and pore properties, the PEI loading amount as well as the sorption temperature were examined. Furthermore, polyethylene glycol(PEG) was introduced as an additive into the sorbents and its effect was investigated at different PEI loadings and sorption temperatures. The results suggest that the pore properties of MBS(after PEI loading) play a more important role in the CO_2 sorption capacity, rather than those of the supports alone.MBS with 3D pore structure exhibits higher CO_2 sorption capacity and amine efficiency than those with 2D-structured support. Among the sorbents studied, fumed silica(HS-5) based MBS showed the highest CO_2 sorption capacity in the temperature range of 30-95 °C, probably due to its unique interstitial pores formed by the aggregation of polymer-loaded SiO_2 particles. It was found that the temperature dependence is directly related to the PEI surface coverage layers. The more PEI surface coverage layers, the higher diffusion barrier for CO_2 and the stronger temperature dependence of CO_2 capacity. 3D MBS exceeds 2D MBS at the same PEI coverage layers due to lower diffusion barrier. Adding PEG can significantly enhance the CO_2 sorption capacity and improve amine efficiency of all MBS, most likely by alleviating the diffusion barrier within PEI bulk layers through the inter-molecular interaction between PEI and PEG.
基金Shanghai Science and Technology Committee Project,China(No.18490740400)Open Foundation of Key Laboratory of Science&Technology of Eco-Textile,China(No.Eco-KF-201612)+1 种基金Opening Project of Zhejiang Provincial Preponderant and Characteristic Subject of Key University,Zhejiang Chinese Medical University,China(No.ZYAOX2018035)Project of Health and Family Planning Commission of Zhejiang Province,China(No.2018KY831)
文摘A novel composite carrier of folic acid(FA)-polyethyleneimine(PEI)-ethosome(Eth)(FA-PEI-Eth)was developed for the treatment of cancers through loading and targeting delivery of multidrug(including gene and other drugs)into cancer cells.Physical and chemical property tests were done to prove the grafting of the composite.Gel retardation test was done to determine the optimal ratio of DNA@PEI complex,and cytocompatibility tests and tumor cell uptake tests were done to evaluate the efficiency of the composite.The results demonstrated that the FA-PEI-Eth could effectively deliver a gene and other drugs into tumor cells simultaneously,and suggested that this composite would be a promising carrier in tumor-targeted therapy applications.
基金supported by the National Natural Science Foundation of China(Grant Nos.20506001 and 20236020)the Program for New Century Excellent Talents in University(Grant No.NCET-04-0123)Beijing Municipal Commission of Education(Grant No.JD100100403).
文摘In this study,two different methods were applied to disperse hollow silica nanoparticles(HSNP);one employed polyethylenimine(PEI)as the dispersant during the synthesis processes for preparing HSNP,while the other added PEI into suspensions of the prepared HSNP and used milling treatment to achieve the desired dispersion.It was found that adding PEI during the synthesis process of HSNP had no noticeable improvement in the dispersion,while adding PEI into suspen-sions of the prepared HSNP and utilizing milling treatment resulted in remarkable dispersion improvement.Therefore,the latter was chosen as the method in dispersing HSNP suspensions.The adsorption of PEI on the surface of HSNP and the stability of the aqueous suspensions was investigated.The results indicated that the adsorption of PEI on the surface of HSNP would increase the repulsive energy among particles,hence reducing the agglomeration of HSNP and improving the stability of the aqueous suspensions.The change of HSNP’s ■ potential after adding PEI and the rela-tionship between the adsorbed amount of PEI and pH were also investigated.
基金by NIH Grants(P20 GM072069,R01 CA108468-01,U01 HL080711,and U54CA119338)the Georgia Cancer Coalition Distinguished Cancer Scholars Program(to S.N.).
文摘Semiconductor quantum dots(QDs)have shown great promise as fluorescent probes for molecular,cellular and in vivo imaging.However,the fluorescence of traditional polymer-encapsulated QDs is often quenched by proton-induced etching in acidic environments.This is a major problem for applications of QDs in the gastrointestinal tract because the gastric(stomach)environment is strongly acidic(pH 12).Here we report the use of proton-resistant surface coatings to stabilize QD fl uorescence under acidic conditions.Using both hyperbranched polyethylenimine(PEI)and its polyethylene glycol derivative(PEG-grafted PEI),we show that the fl uorescence of coreshell CdSe/CdS/ZnS QDs is effectively protected from quenching in simulated gastric fluids.In comparison,amphiphilic lipid or polymer coatings provide no protection under similarly acidic conditions.The proton-resistant QDs are found to cause moderate membrane damage to cultured epithelial cells,but PEGylation(PEG grafting)can be used to reduce cellular toxicity and to improve nanoparticle stability.
基金funding support from the National Natural Science Foundation of China(No.52033006 and 51873142)Suzhou Science and Technology Development Project(No.SYS2019072) Science Foundation of China (No. 52033006 and 51873142)+1 种基金Suzhou Science and Technology Development Project (No.SYS2019072), Collaborative Innovation Center of Suzhou NanoScience & Technology, the 111 project, Suzhou Key Laboratory ofNanotechnology and BiomedicineJoint InternationalResearch Laboratory of Carbon-Based Functional Materials andDevices。
文摘Myocardial ischemia reperfusion(IR)injury is closely related to the overwhelming inflammation in the myocardium.Herein,cardiomyocyte-targeted nanotherapeutics were developed for the reactive oxygen species(ROS)-ultrasensitive co-delivery of dexamethasone(Dex)and RAGE small interfering RNA(siRAGE)to attenuate myocardial inflammation.PPTP,a ROSdegradable polycation based on PGE2-modified,PEGylated,ditellurium-crosslinked polyethylenimine(PEI)was developed to surface-decorate the Dex-encapsulated mesoporous silica nanoparticles(MSNs),which simultaneously condensed siRAGE and gated the MSNs to prevent the Dex pre-leakage.Upon intravenous injection to IR-injured rats,the nanotherapeutics could be efficiently transported into the inflamed cardiomyocytes via PGE2-assisted recognition of over-expressed E-series of prostaglandin(EP)receptors on the cell membranes.Intracellularly,the over-produced ROS degraded PPTP into small segments,promoting the release of siRAGE and Dex to mediate effective RAGE silencing(72%)and cooperative antiinflammatory effect.As a consequence,the nanotherapeutics notably suppressed the myocardial fibrosis and apoptosis,ultimately recovering the systolic function.Therefore,the current nanotherapeutics represent an effective example for the codelivery and on-demand release of nucleic acid and chemodrug payloads,and might find promising utilities toward the synergistic management of myocardial inflammation.
基金Project supported by the National Natural Science Foundation of China (No. 50873089)the Natural Science Foundation of Zhejiang Province, China (No. Y407173)
文摘The calcium phosphate (CaP) particles have attracted much attention in gene therapy. How to construct stable gene particles was the determining factor. In this study,hybrid multi-shell CaP gene particles were successfully constructed. First,CaP nanoparticles served as a core and were coated with DNA for colloidal stabilization. The ξ-potential of DNA-coated CaP nanoparticles was -15 mV. Then polyethylenimine (PEI) was added and adsorbed outside of the DNA layer due to the electrostatic attraction. The ξ-potential of hybrid multi-shell CaP particles was slightly positive. With addition of PEI,the hybrid multi-shell particles could condense DNA effectively,which was de-termined by ethidium bromide (EtBr) exclusion assay. The hybrid particles were spherical and uniform with diameters of about 150 nm at proper conditions. By simple modification of PEI,the hybrid multi-shell CaP gene particles were successfully constructed. They may have great potential in gene therapy.
