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Pb胁迫对欧美杂交杨(Populus deltoides×Populus nigra)生物量分配格局及其Pb富集特性的影响 被引量:5
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作者 康丽娜 吴福忠 +4 位作者 何振华 徐振锋 刘洋 杨万勤 杨玉莲 《农业环境科学学报》 CAS CSCD 北大核心 2012年第3期484-490,共7页
采用速生树种修复重金属污染土壤的方法近年来受到越来越多关注,但已有结果存在很大不确定性。为了解杨树在不同Pb胁迫条件下生长响应和Pb富集效果,以长江上游两种典型土壤(酸性紫色土和钙质紫色土)为栽培介质,采用盆栽试验方法,研究了... 采用速生树种修复重金属污染土壤的方法近年来受到越来越多关注,但已有结果存在很大不确定性。为了解杨树在不同Pb胁迫条件下生长响应和Pb富集效果,以长江上游两种典型土壤(酸性紫色土和钙质紫色土)为栽培介质,采用盆栽试验方法,研究了不同Pb浓度处理下(CK:0mg·kg-1;T1:200mg·kg-1;T2:450mg·kg-1;T3:2000mg·kg-1)欧美杂交杨(Populus deltoides×Populusnigra)生物量生产与分配格局以及Pb吸收、富集特性。两种土壤条件下杨树各器官生物量及总生物量均表现出随Pb胁迫程度的增加而降低的趋势,Pb胁迫条件下杨树生物量分配格局在钙质紫色土中表现为茎>粗根>叶>细根。相同浓度Pb处理条件下,单株杨树总生物量均表现为钙质紫色土大于酸性紫色土。随着Pb处理浓度的增大,杨树各器官Pb含量及积累量显著增加。Pb胁迫使杨树对Pb的富集系数逐渐增大而耐性系数逐渐减小。T3处理条件下杨树对Pb的富集系数在酸性紫色土中较大,且各处理条件下杨树对Pb的耐性系数均为酸性紫色土中较大。这些结果表明,高浓度Pb胁迫条件下酸性紫色土中的欧美杂交杨表现出较好的吸收和富集Pb的特性,这为Pb污染土壤的生物修复提供了一定的科学依据。 展开更多
关键词 欧美杂交杨 PB胁迫 酸性紫色土 钙质紫色土 生物修复
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镉胁迫对欧美杂交杨(Populus deltoides×Populus nigra)光合作用与生物量生产的影响 被引量:5
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作者 沈迪玉 杨万勤 +2 位作者 张健 周利强 吴福忠 《四川农业大学学报》 CSCD 北大核心 2010年第1期61-66,共6页
采用盆栽实验研究了生长在四川盆地典型Cd污染冲积土和紫色土上的欧美杂交杨(Populus deltoides×Populus nigra)对国家土壤环境质量标准中Cd浓度梯度(0、0.3、1.0、2.0 mg/kg)的光合响应和生物生产量。Cd处理显著降低紫色土上生长... 采用盆栽实验研究了生长在四川盆地典型Cd污染冲积土和紫色土上的欧美杂交杨(Populus deltoides×Populus nigra)对国家土壤环境质量标准中Cd浓度梯度(0、0.3、1.0、2.0 mg/kg)的光合响应和生物生产量。Cd处理显著降低紫色土上生长的杨树叶绿素a和叶绿素总量,增加气孔导度与胞间CO2浓度,增加凋落叶生物量,降低茎生物量、根生物量和单株总生物量。但是,Cd处理对冲积土上生长的杨树叶绿素特征、光合生理指标和生物量生产并无明显差异。尽管同一生长基质上不同Cd处理间杨树光合生理指标与生物量生产均有一定程度的影响,但紫色土上生长的杨树生理和生长特征均显著低于冲积土上生长的杨树。这些结果表明,自然条件国家土壤环境质量标准内,Cd污染并不对杨树生长构成较大威胁,而生长土壤基质的选择可能对杨树的栽培更为重要。这为Cd污染土壤区域城市森林建设和速生丰产林建设提供了一定的科学依据。 展开更多
关键词 欧美杂交杨 CD 光合作用 紫色土 冲积土 生物量生产
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Assembly and analysis of the Populus deltoides mitochondrial genome:the first report of a multicircular mitochondrial conformation for the genus Populus
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作者 Yanshu Qu Pengyan Zhou +2 位作者 Chunfa Tong Changwei Bi Li’an Xu 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第3期717-733,共17页
Genomics research of Populus deltoides,an important timber species that is widely planted worldwide,is an important part of poplar breeding.Currently,the nuclear and chloroplast genome of P.deltoides have been sequenc... Genomics research of Populus deltoides,an important timber species that is widely planted worldwide,is an important part of poplar breeding.Currently,the nuclear and chloroplast genome of P.deltoides have been sequenced,but its mitochondrial genome(mitogenome)has not been reported.To further explore the evolution and phylogeny of P.deltoides,the mitogenome of P.deltoides I-69 was assembled using reads from Nanopore and Illumina sequencing platforms and found to consist of 802,637 bp and three circular chromosomes(336,205,280,841,and 185,591 bp)containing 58 genes(34 protein-coding genes,21 tRNA genes,and 3 rRNA genes).RNA analysis in combination with several species showed signifi cantly fewer RNA editingsites in the mitogenomes of poplar and other angiosperms than in gymnosperms.Sequence transfer analysis showed extensive mitogenome rearrangements in Populus species,and with evolution from lower to higher plants,tRNA transfer from chloroplasts to mitochondria became increasingly frequent.In a phylogenetic analysis,the evolutionary status of P.deltoides was determined,and the section Populus was supported.Our results based on the fi rst report of a multicircular conformation of the Populus mitogenome provide a basis for further study of the evolution and genetics of P.deltoides and other Populus species and for breeding programs. 展开更多
关键词 Mitochondrial genome Multi-circular populus deltoids Comparative genomics PHYLOGENY
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美洲黑杨(Populus deltoides Marsh)×青杨(P.cathayana Rehd.)分子连锁图谱的构建 被引量:35
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作者 苏晓华 张绮纹 +1 位作者 郑先武 张香华 《林业科学》 CAS CSCD 北大核心 1998年第6期29-27,共1页
利用随机扩增DNA多态性RAPD方法,在美洲黑杨(P.deltoidesMarsh)×青杨(P.cathayanaRehd.)3代谱系中分析分子标记,构建出第1张美洲黑杨×青杨分子连锁图谱。共从300个10... 利用随机扩增DNA多态性RAPD方法,在美洲黑杨(P.deltoidesMarsh)×青杨(P.cathayanaRehd.)3代谱系中分析分子标记,构建出第1张美洲黑杨×青杨分子连锁图谱。共从300个10mer随机引物中筛选出79个适合引物,检测出可供构图的分离标记180个。该图谱由20个连锁群,110个RAPD标记组成。总图距为覆盖基因组总长度的7035%,标记间的平均间距为1727cM.连锁群长度在371~1898,相应标记分别在3~10。本图谱为杨树抗病、虫和其它性状基因定位提供了框架结构,为实现杨树分子遗传育种迈进了最重要的一步。 展开更多
关键词 分子连锁图 RAPD 美洲黑杨 青杨
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南洞庭湖杨树(Populus deltoides)清理迹地恢复初期涨水前后土壤种子库特征 被引量:8
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作者 刘静逸 牛艳东 +4 位作者 郭克疾 蒋丽娟 罗学卫 吴小丽 徐佳娈 《湖泊科学》 EI CAS CSCD 北大核心 2021年第2期539-549,共11页
本文研究了南洞庭湖杨树清理1 a、2 a后的迹地(简称1 a和2 a)和未清理杨树洲滩(ck)涨水前后土壤种子库特征变化及其与地上植被的关系.结果显示:杨树清理迹地土壤种子库共萌发物种23科59属65种,涨水前后土壤种子库密度和种类均表现出1 a&... 本文研究了南洞庭湖杨树清理1 a、2 a后的迹地(简称1 a和2 a)和未清理杨树洲滩(ck)涨水前后土壤种子库特征变化及其与地上植被的关系.结果显示:杨树清理迹地土壤种子库共萌发物种23科59属65种,涨水前后土壤种子库密度和种类均表现出1 a>2 a>ck的趋势,且随着土层加深而递减.退水后ck、1 a、2 a不同土层物种数量和密度较涨水前均有所减少,其中一或二年生物种较涨水前有所减少,多年生物种占比增加;湿生物种与中生物种数目较涨水前均有所减少,而2 a土壤种子库中生物种比例较涨水前有所增加.涨水前后土壤种子库Shannon-Wiener和Simpson指数变化较小,退水后Margalef指数较涨水前有所下降.退水后地上植被物种Shannon-Wiener和Simpson指数高于涨水前地上植被,而Margalef指数有所下降.涨水前Srensen相似性系数表现为1 a>2 a>ck的趋势,退水后表现为2 a>1 a>ck的趋势,涨水前后不同恢复年份土壤种子库和地上植被优势物种所占比例有所差异.研究表明,南洞庭湖杨树清理迹地退水后土壤种子库密度和种类显著下降,随着恢复年份和水淹频次的增加,一或二年生物种迅速减少,多年生物种比例增加,地上植被物种均匀性有所增加,可见水文因子是影响南洞庭湖杨树清理迹地种子库恢复能力的重要因素. 展开更多
关键词 南洞庭湖 杨树 清理迹地 涨水 土壤种子库
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Isolation and characterization of two genes encoding polygalacturonase-inhibiting protein from Populus deltoides 被引量:9
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作者 Qiang Cheng Youzhi Cao Huixin Pan Mingxiu Wang Minren Huang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第10期631-638,共8页
Polygalacturonase-inhibiting proteins (PGIPs) are extracellular proteins that belong to the leucine-rich repeat (LRR) protein superfamily. PGIPs inhibit fungal polygalacturonases (PGs) and promote accumulation o... Polygalacturonase-inhibiting proteins (PGIPs) are extracellular proteins that belong to the leucine-rich repeat (LRR) protein superfamily. PGIPs inhibit fungal polygalacturonases (PGs) and promote accumulation of oligogalacturonides, which activate plant defense responses. PGIPs play important roles in resistance to infection of pathogens. In this study, reverse transcriptase-polymerase chain reaction (RT-PCR) and RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) were used to isolate the full-length PGIP cDNA from Populus deltoides (GenBank accession no. of PdPGIP2 and PdPGIP4:EF684913 and EF684912). Domain analysis revealed that the deduced amino acid sequences of PdPGIP2 and PdPGIP4 had a typical PGIP topology. Phylogenetic analysis of known PGIPs indicated that the two PdPGIPs were clustered to the defense-related PGIP clade. Using real-time RT-PCR, the expression patterns of the two PdPGIPs following treatment with a fungal pathogen and defense-related signaling molecules were studied. The expression levels of PdPGIP2 and PdPGIP4 were both up-regulated when inoculated with the phytopathogenic fungus Marssonina brunnea. Therefore, it was proposed that the two PGIPs might be involved in the resistance to Marssonina brunnea in P. deltoides. 展开更多
关键词 Polygalacturonase-inhibiting protein real-time RT-PCR populus deltoides
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Morphological and physiological plasticity response to low nitrogen stress in black cottonwood(Populus deltoides Marsh.) 被引量:1
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作者 Cun Chen Yanguang Chu +5 位作者 Qinjun Huang Changjun Ding Weixi Zhang Bo Li Jing Zhang Xiaohua Su 《Journal of Forestry Research》 SCIE CAS CSCD 2022年第1期51-62,共12页
It is important to evaluate nitrogen use efficiency and nitrogen tolerance of trees in order to improve their productivity.In this study,both were evaluated for 338 Populus deltoides genotypes from six provenances.The... It is important to evaluate nitrogen use efficiency and nitrogen tolerance of trees in order to improve their productivity.In this study,both were evaluated for 338 Populus deltoides genotypes from six provenances.The plants were cultured under normal nitrogen(750μM NH_4 NO_3)and low nitrogen(5μM NH_4 NO_3)conditions for 3 months.Growth,chlorophyll content and glutamine synthetase activity of each genotype were measured.Under low nitrogen,heights,ground diameter,leaf area,leaf and root biomass,and chlorophyll contents were significantly lower than those under normal nitrogen level.Correlation analysis showed that nutrient distribution changed under different nitrogen treatments.There was a negative correlation between leaf traits and root biomass under normal nitrogen level,however,the correlation became positive in low nitrogen treatment.Moreover,with the decrease of nitrogen level,the negative correlation between leaf morphology and chlorophyll levels became weakened.The growth of the genotypes under the two treatments was evaluated by combining principal component analysis with a fuzzy mathematical membership function;the results showed that leaf traits accounted for a large proportion of the variation in the evaluation model.According to the results of comprehensive evaluation of plants under the two treatments,the 338 P.deltoides genotypes could be divided into nine categories,with wide genotypic diversity in nitrogen use efficiency and low nitrogen tolerance.As a result,26 N-efficient genotypes and 24 N-inefficient genotypes were selected.By comparative analysis of their morphological and physiological traits under the two treatments,leaf traits could be significant indicators for nitrogen use efficiency and nitrogen tolerance,which is of considerable significance for breeding poplar varieties with high nitrogen use efficiencies. 展开更多
关键词 populus deltoides Nitrogen deficiency Nitrogen use efficiency Comprehensive evaluation Genotypic diversity
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Study on Phenological Character in Seedling Period of Introduced Populus deltoids Clones 被引量:2
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作者 唐洁 汤玉喜 +4 位作者 苏晓华 李永进 黄秦军 吴敏 杨艳 《Agricultural Science & Technology》 CAS 2014年第3期422-426,共5页
In order to provide directionally genetically improved breeding materials of poplar by exploring the phenological traits genetic variation level and its develop- ment potential of Populus deltoides and the resource ev... In order to provide directionally genetically improved breeding materials of poplar by exploring the phenological traits genetic variation level and its develop- ment potential of Populus deltoides and the resource evaluation was carried out; 8 phenological phases in seedling period were observed and analyzed of 60 Populus deltoids clones introduced from America. The results showed that: (1) there was obvious difference in phonological character among clones, especially in leaf-spread- ing peak stage and the end term of leaf-falling stage, with the largest variation co- efficient of 14.97% and the minimum of 3.83% respectively. (2) Leaf-spreading peak stage scattered but the end term of leaf-falling stage concentrated the most. The phonological character in early stage of seedling growth was the main factor influ- encing the length of growing season. (3) By principal component analysis, pheno- logical phases were classified into 3 typical periods, including germination stage, leaf-spreading peak stage and leaf-falling stage. (4) Totaling 60 clones were classi- fied into 4 types by using clustering analysis in phenological time variables of clones. 展开更多
关键词 populus deltoids CLONE Phenological phase Principal component analysis Clustering analysis
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Foraging behavior of parasitoid chalcid to the essential oil from bark of Populus pseudo-simonii P. nigra and Quadraspidiotus gigas 被引量:2
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作者 迟德富 Rafael Ocete Rubio +2 位作者 严善春 张丹丹 温振宏 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第4期255-259,共5页
采用四臂式嗅觉仪测定了长棒四节蚜小蜂(Pteroptrix longgiclava(Girault)(Hymenoptera:Aphelinidae))和黄胸扑虱蚜小蜂(Encarsia gigas(Tshumakova)(Hymenoptera:Aphelinidae)),对健康的小青×黑杨(Populus pseudo-simonii×... 采用四臂式嗅觉仪测定了长棒四节蚜小蜂(Pteroptrix longgiclava(Girault)(Hymenoptera:Aphelinidae))和黄胸扑虱蚜小蜂(Encarsia gigas(Tshumakova)(Hymenoptera:Aphelinidae)),对健康的小青×黑杨(Populus pseudo-simonii×P.nigra)树皮、被杨圆蚧(Quadraspidiotus gigas(Thiem & Gerneck))危害的杨树皮、杨圆蚧1齿固定若虫的虫体和介壳的挥发油的趋性反应。测定结果表明:被杨圆蚧危害后的小青×黑杨树皮和杨圆蚧1龄因定若虫的介壳对长棒四节蚜小蜂和黄胸扑虱蚜小蜂成虫具有较大的吸引力。3-7μL介壳挥发油和5-9μL受害杨树皮挥发油对长棒四节蚜小蜂的寄主搜寻行为具有较强的引导作用。虫体的挥发油对小蜂的寄生定位也起到了一定的引诱作用。两种小蜂对健康树皮的挥发油几乎没有反应。图4表1参20。 展开更多
关键词 populus pseudo-simonii ×P. nigra Quadraspidiotus gigas (Thiem & Gerneck) Pteroptrix longgiclava (Girault) Encarsia gigas (Tshumakova) Essential Oil PARASITOID Foraging behavior
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Characterization and identification of leaf morphology of Populus deltoides Bartr. clones
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作者 Ajaz LONE Altamah BASHIR +1 位作者 Salil K. TEWARI Mumtaz MAJEED 《Forestry Studies in China》 CAS 2011年第4期270-275,共6页
Leaves are of fundamental importance to plants, representing their facility to generate power and are the sensing units of plants towards the environment. An attempt was made to characterize and compare the variations... Leaves are of fundamental importance to plants, representing their facility to generate power and are the sensing units of plants towards the environment. An attempt was made to characterize and compare the variations of leaf morphology of various Populus deltoides Bartr. clones by studying the winter buds and other leaf parameters of fully developed leaves. To achieve these objectives, forty-three exotic and indigenous clones ofP. deltoides Bartr. were evaluated for different parameters. On the basis of various morphological characteristics the results reveal that each clone has a distinct color pattern of leaves. Different colors observed in these clones varied from light green through green to dark green. Two distinct lengths of the leaf apex were found, i.e., short and long; as well both acuminate and acute apex types were found. Erratic distribution of serration of leaves was also found. In this study, the morphological traits of leaves provided discriminatory grounds for separating various populations of P. deltoides Bartr. clones. Winter bud studies indicate that different clones vary considerably with regard to shape, color, shape of leaf scars and exudation. 展开更多
关键词 populus deltoides Bartr. LEAVES MORPHOLOGY winter buds
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Compressive Strength Parallel to Grain Variation of Different Clones of Populus deltoides Marsh.
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作者 Yan YANG Yuxi TANG +1 位作者 Jie TANG Yongjin LI 《Agricultural Biotechnology》 CAS 2018年第4期172-174,177,共4页
In order to explore the differences in the compressive strength parallel to grain (CSPG) between different clones and individual woods of Populus deltoides Marsh., the CSPGs of 5 15-year-old P. deltoides clones were... In order to explore the differences in the compressive strength parallel to grain (CSPG) between different clones and individual woods of Populus deltoides Marsh., the CSPGs of 5 15-year-old P. deltoides clones were determined and analyzed. The results showed that there were significant differences in CSPG of wood between different clones of P. deltoides . The CSPG of the tested clones reached more than 41 MPa, which was higher than the average compressive strength of P. deltoides (32.94 MPa). Similar to the changes of stem height, the CSPG of the clones gradually increased with the increase of stem height. However, there were significant differences in CSPG of an individual plant at different tree heights. The CSPG of P. deltoides clones was related with their growth traits, but not significantly. Therefore, it is expected to achieve simultaneous and independent improvement on CSPG and the growth of P. deltoides , so as to get excellent individuals with fast growth and high CSPG. 展开更多
关键词 populus deltoides Marsh. CLONE Stem height Compressive strength parallel to grain
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Structural Association of Endoplasmic Reticulum with Other Membrane Systems in Populus deltoides Apical Bud Cells and Its Alterations During the Short Day_induced Dormancy 被引量:15
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作者 简令成 李积宏 +1 位作者 李本湘 陈辉煌 《Acta Botanica Sinica》 CSCD 2000年第8期803-810,共8页
Electron microscopic observations revealed that the tissues of poplar ( Populus deltoides Bartr. ex Marsh) apical bud cells, which were fixed by a modified procedure of potassium permanganate fixative, showed a di... Electron microscopic observations revealed that the tissues of poplar ( Populus deltoides Bartr. ex Marsh) apical bud cells, which were fixed by a modified procedure of potassium permanganate fixative, showed a distinct endomembrane organization, in particular, the structural associations of the endoplasmic reticulum (ER) with other membrane systems. The striking findings are that some ER elements were in connection with the nuclear envelopes of two adjacent cells through plasmodesmata, and many ER elements were also associated with mitochondria, plastids, Golgi bodies or the plasma membrane (PM), forming a bridge_like continuum among various endomembrane systems or between nucleus to nucleus. A great number of plasmodesmata existed between cells, indicating a perfectly integrated symplasmic structure in poplar apical bud meristem grown in a long day environment. During the short day_induced dormancy, ER contracted, leading to its disassociation between nuclei, and between the nucleus and organelles/plasmalemma in many cells. After dormancy broke and shoots growth resumed, contracted ER was no longer observed in the apical bud cells. The ER associations with other endomembrane systems and the intercellular communication channels were re_established similar to that of plants before dormancy induction. These observations suggest that ER may play an important role in linking_up between the nucleus and organelles, and between the nucleus and the nucleus (or cell_to_cell), and seemingly coordinating various physiological processes by the bridging_like associations. And the contraction of ER under short_day may result in the growth cessation and the development of dormancy in poplar. 展开更多
关键词 endoplasmic reticulum ER_bridge association endoplasmic reticulum contraction PLASMODESMA DORMANCY populus deltoides
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Effects of Fertilization on Young Populus deltoids cv. Zhonghua Hongye Growth
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作者 闵安民 易培军 +4 位作者 张锡九 蔡小虎 王宇 王丽 李红霞 《Agricultural Science & Technology》 CAS 2016年第6期1341-1344,共4页
Using complete and orthogonal design, fertilization trials were conducted for the young growth of Populus deltoids cv. Zhonghua hongye in the fluviogenic soil in Xindu District, Chengdu City. The results showed that t... Using complete and orthogonal design, fertilization trials were conducted for the young growth of Populus deltoids cv. Zhonghua hongye in the fluviogenic soil in Xindu District, Chengdu City. The results showed that the application ef- fect of the single nutrient elements ranked as K〉N〉P, and that of the fertilization combinations ranked as N+P+K〉N+K〉N+P, P+K. The optimum fertilization formula was urea [(NH2)2CO, 150 g/plant] + calcium superphosphate [Ca(H2PO4)2.H20, 220 g/plant] + potassium sulphate (K2SO4, 75 g/plant). 展开更多
关键词 populus deltoids cv. Zhonghua hongye Young Growth Fertilization formula Effect
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Two novel eukaryotic translation initiation factor 5A genes from Populus simonii×P.nigra confer tolerance to abiotic stresses in Saccharomyces cerevisiae 被引量:4
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作者 Tangchun Zheng Lina Zang +2 位作者 Lijuan Dai Chuanping Yang Guanzheng Qu 《Journal of Forestry Research》 SCIE CAS CSCD 2017年第3期453-463,共11页
The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using th... The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized PsneIFSA2 and PsneIFSA4 from young poplar (P. simonii × P. nigra) leaves. The deduced amino acid sequences of PsneIF5A2 and PsneIF5A4 were 98 % similar to each other, and they are orthologs of eIF5A 1 in Arabidopsis. In a subcellular localization analysis, PsneIF5A2 and PsneIF5A4 proteins were localized in the nucleus and cytoplasm, qRT-PCR analysis showed that PsneIF5A2 and PsneIF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing PsneIF5A2 and PsneIF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that PsneIF5A2 and PsneIF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants. 展开更多
关键词 Abiotic tolerance EIF5A populus simonii × P. nigra Subcellular localization Yeast
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Influences of the Populus deltoids seedlings treated with exogenous methyl jasmonate on the growth and development of Lymantria dispar larvae 被引量:2
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作者 HU Zeng-hui ZHAO Ling +2 位作者 YANG Di SHEN Ying-bai SHEN Fan-yi 《Journal of Forestry Research》 SCIE CAS CSCD 2006年第4期277-280,共4页
Lyantria dispar larvae were fed with the leaves of Populus deltoids seedlings exposed to methyl jasmonate (MeJA) for 24 h. The growth and development of the larvae were investigated, and phenolics contents in treate... Lyantria dispar larvae were fed with the leaves of Populus deltoids seedlings exposed to methyl jasmonate (MeJA) for 24 h. The growth and development of the larvae were investigated, and phenolics contents in treated leaves including pyrocatechol, caffeic acid, coumarin, fernlic acid and benzoic acid were also surveyed by high-pressure liquid chromatography (HPLC). Results indicated that approximate digestibility, efficiency of conversion of ingested food, efficiency of conversion of digested food, and weight of the larvae were inhibited obviously, especially from the sixth day, which may result from the increase of total phenolics contents in treated leaves. This result provides strong supports for MeJA acting as the airborne signal molecule between woody plants. 展开更多
关键词 Interplant communication Defense response in populus deltoids plants Exogenous MeJA Growth and development of lymantria dispar Phenolics contents
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Pharmacological potential of Populus nigra extract as antioxidant,anti-inflammatory,cardiovascular and hepatoprotective agent 被引量:1
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作者 Nadjet Debbache-Benaida Dina Atmani-Kilani +2 位作者 Valerie Barbara Schini-Keirth Nouredine Djebbli Djebbar Atmani 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2013年第9期697-704,共8页
Objective:To evaluate antioxidant,anti-inflammatory,hepatoprotective and vasorelaxant activities of Pupulus nigra flower buds ethanolic extract.Methods:Antioxidant and anti-inflammatory activities of the extract were ... Objective:To evaluate antioxidant,anti-inflammatory,hepatoprotective and vasorelaxant activities of Pupulus nigra flower buds ethanolic extract.Methods:Antioxidant and anti-inflammatory activities of the extract were assessed using respectively the ABTS test and the animal model of carrageenan-induced paw edema.Protection from hepatic toxicity caused by aluminum was examined by histopathologic analysis of liver sections.Vasorelaxant effect was estimated in endothelium-intact and-nibbed rings of porcine coronary arteries precontracted with high concentration of U466I9.Results:The results showed a moderate antioxidant activity(40%),but potent anti-inflammatory activity(49.9%)on carrageenan-induced mice paw edema,and also as revealed by histopathologic examination,complete protection against AlCl_3-induced hepatic toxicity.Relaxant effects of the same exlracl on vascular preparation from porcine aorta precontracted with high concentration of U466I9 were considerable at 10^(-1)g/L,and comparable(P>0.05)between endothelium-intact(67.74%,IC_(50)0.04 mg/ml.)and-rubbed(72.72%,IC_(50)=0.075 mg/ml,)aortic rings.Conclusions:The extract exerted significant anti-inflammatory,hepatoprotective and vasorelaxant activities,the latter being cndothelium-independent believed to be mediated mainly by the ability of components present in the extract to exert antioxidant properties,probably related to an inhibition of Ca^(2+)influx. 展开更多
关键词 populus nigra POLYPHENOLS ANTI-INFLAMMATORY activity HEPATOPROTECTION VASORELAXATION
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Association Analysis of the Growth of Black Poplar (Populus Nigra L.) Under Contrasting Nitrogen Levels 被引量:1
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作者 Xihua Liu Changjun Ding Xiaohua Su 《Phyton-International Journal of Experimental Botany》 SCIE 2019年第4期425-433,共9页
The European black poplar(Populus nigra L.)has been used as a germplasm resource for the breeding of new poplar varieties around the world.The identification and screening of its high nitrogen use efficiency genotypes... The European black poplar(Populus nigra L.)has been used as a germplasm resource for the breeding of new poplar varieties around the world.The identification and screening of its high nitrogen use efficiency genotypes could enable the breeding of new resource-efficient poplar varieties.The accessions were screened using MALDI-TOF MS genotyping technology for ammonium transporter(AMT)and nitrate transporters(NRT)genes against phenotypic data for seedling height and ground diameter traits,in both low and high nitrogen environments.Allele re-sequencing of seven genes related to root development was carried out using the minisequencing method.By cluster analysis,101 accessions of black poplar were divided into 4 populations,and it was concluded that Central Europe is the origin of the evolution of low-nitrogen and high-efficiency populations of European black poplar.Association study between SNP typing and seedling height and ground diameter traits showed that there were significant correlations between four SNP loci and growth traits under the contrasting N levels.We found that SNP3 and SNP4 in the PttAMT1;3 gene were significantly associated with seedling height traits,and that SNP2 and SNP7 in the PttAMT1;2 and PttAMT1;5 genes,respectively,were significantly associated with ground diameter traits.Thus,considerable allelic diversity is present within the candidate genes studied and can be utilized to develop functional markers to select for poplars with improved growth under N stress conditions. 展开更多
关键词 NITROGEN populus nigra SNP association analysis
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No consistent daily variation in DNA methylation detected in Populus nigra leaves by methylation-sensitive amplification polymorphism analysis
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作者 Shu Diao Yanbo Wang +5 位作者 Changjun Ding Yingying Chang Lixiong Liang Yanan Gao Bingyu Zhang Xiaohua Su 《Journal of Forestry Research》 SCIE CAS CSCD 2017年第4期653-660,共8页
DNA methylation, an epigenetic mechanism used by cells to control gene expression, has an important biological role in plant development and environmental fitness. Since plant DNA methylation is closely related to env... DNA methylation, an epigenetic mechanism used by cells to control gene expression, has an important biological role in plant development and environmental fitness. Since plant DNA methylation is closely related to environmental conditions, variation during the day is expected. Here, in genetically identical plants of Populus nigra clone N46, DNA methylation changes in leaves over a 24 h period were detected using the methylation-sensitive amplification polymorphism method. The results showed different DNA methylation patterns in mature poplar leaves: not only in individuals at the same time, but also in samples at each of the six time during the day. In addition, night samples had a higher percentage of methylation than in morning samples. However, no statistically significant differences were found among the samples gathered at different times. Similar results were obtained for three other P. nigra clones with different genetic backgrounds. Real time qPCR showed that the DNA methyltransferase genes Pt-MET1 and Pt-SOM1 involved in CG DNA methylation in poplar were stable over a 24 h period in leaves of P. nigra N46 compared with circadian-controlled genes. That could be part of the reason that methylation of CCGG sites is stable in those leaves. That DNA methylation differed even in genetically identical plants indicates the specificity of DNA methylation changes in their genomes. No statistically significant differences in methylation changes were found between day and night, suggesting that DNA methylation is more stable than expected and is unlikely to be involved in circadian regulation in plants. 展开更多
关键词 Cytosine methylation EPIGENETIC populus nigra Methylation-sensitive amplification polymorphism (MSAP) Variation
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The effect of number of joints on bending properties of laminated lumber made from poplar (Populus nigra)
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作者 Ali BAYATKASHKOLI Mohammad SHAMSIAN Marjan MANSOURFARD 《Forestry Studies in China》 CAS 2012年第3期246-250,共5页
Connection plays an important role in structural components and end jointing is one of the most common connections in structural applications. This study was designed to investigate some mechanical properties (MOE an... Connection plays an important role in structural components and end jointing is one of the most common connections in structural applications. This study was designed to investigate some mechanical properties (MOE and MOR) of solid wood samples manufactured from poplar (Populus nigra), joined together through end jointing. As well, we studied combinations of lumber manufactured from 10-mm veneer, using a polyvinyl acetate adhesive. The results show that non-jointed lumber (control samples) have better bending properties (MOE and MOR) than jointed specimens and lumber made from laminated layers. On the whole, side-by- side joints have a negative effect on the MOR and MOE of laminated products, which is more pronounced in the MOR. By increasing the number of joints, the properties of combination of three-ply, i.e., bending strength and modulus of elasticity, decreased. 展开更多
关键词 laminated wood products bending properties joint number poplar populus nigra wood
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Transformation of Tobacco with Two AP1 Genes Isolated from Poplar( Populus simonii × Populus nigra)
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作者 Shuang LI Tangchun ZHENG +1 位作者 Lina ZANG Guanzheng QU 《Agricultural Biotechnology》 CAS 2014年第4期23-27,共5页
[ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating t... [ Objective ] This study aimed to analyze the functions of AP1 gene from Populus simonii × Populus nigra and to lay the theoretical foundation for shortening the breeding cycle of forest trees and investigating the flowering mechanism in poplar. [ Method] Plant expression vectors of AP1 genes were constructed and transformed into tobacco leaf disks with Agrobacterium-mediated method. Transgenic tobacco plants were identified by PCR. [ Result] AP1 genes were integrated into the genome of tobacco. Transgenic tobacco plants all presented an early flowering phenotype compared with wild-type tobacco. [ Conclusion] AP1 genes could promote early flowering in transgenic tobacco plants, which provided theoretical basis for molecular regulation of flowering in poplar. 展开更多
关键词 populus simonii ×Popul nigra AP1 TOBACCO Early flowering
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