Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were stud...Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L^-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L^-1 2,4-D. The best subculture medium was MS ＋ 0.8 mg＇L-1 2,4-D ＋ 30 g·L^-1 sucrose with a subculture cycle of seven days.展开更多
Ricinus communis L. is a new copper hyperaccumulator growing on Tonglushan copper mine in Hubei Province, China. This study aimed to establish a suspension cell line of R. communis L. with stable and rapid growth for ...Ricinus communis L. is a new copper hyperaccumulator growing on Tonglushan copper mine in Hubei Province, China. This study aimed to establish a suspension cell line of R. communis L. with stable and rapid growth for further screening of heavy metal-resistant R. communis L. cells and breeding of hyperaccu- mulators. In this study, cell suspension culture conditions were optimized by using orthogonal experimental design with previously induced R. communis L. embyre- genic calluses as experimental materials, to establish the suspension cell line of R. communis L. Under the optimal conditions, growth curves of suspension cells and changes in pH of culture liquid were determined. The results showed that the optimal culture conditions for R. conmmnis L. suspension cell line were ： MS ＋ O. 5 rag/I, 6-BA ＋ O. 2 mg/L NAA ＋ 50 mg/L sucrose ＋ 350 mg/L casein hydrolysate as basic medium, with callus inoculation amount of 2.5 g/50 ml, dark culture at （26 ±2） ℃ with shaking at 110 r/min. Under these conditions, increment of fresh weight and dry weight ofR. commun/s L. suspension cells reached the maximum of 4.56 g/（50 ml 14 d） and 0.49 g/（50 ml 16 d）, respectively. Growth curves of R. communis L. suspension cells were basically in ＂S＂ shape. Each culture cycle lasted 16 d, and the rapid growth stage was from the 6th d to the 14th d. In a culture cycle, pH of the culture liquid declined first and then increased to the maximum and stabilized gradually.展开更多
Suspension cultures initiated from callus derived from petiole explants of aspen hybrid （Populus tremuloides×P. tremula） produced somatic embryos. Callus was induced on a MS medium supplemented with 5mg·L^...Suspension cultures initiated from callus derived from petiole explants of aspen hybrid （Populus tremuloides×P. tremula） produced somatic embryos. Callus was induced on a MS medium supplemented with 5mg·L^-1 2,4-D and 0.05mg·L^-1 zeatin under light conditions. Embryogenic calli were obtained when a subsequent subculture of calli was suspended in the same basal medium with 10mg·L^-1 2,4-D. The highest number of globular embryos were induced from embryogenic calli by cell suspension culture in a MS liquid medium supplemented with 10mg·L^-1 2,4-D. Genotype and 2,4-D concentration were vital to the induction of embryogenic calli producing competent cells. Embryogenic calli for each genotype were heterogeneous. Green calli with gel-like consistency could yield more competent cells than light yellow embryogenic calli. However, some globular embryos broke into slices and some developed abnormally after one month of culture under the same or other hormonal conditions.展开更多
本文报道了小黑杨(Populus xiaohei T.S.Hwang ex C.Wang et Tung)细胞培养及植株再生过程,包括花粉愈伤组织的诱导、继代培养、单细胞分离及培养、愈伤组织的再形成及植株再生。试验结果表明:①悬浮细胞的质量是影响细胞培养的根本因子...本文报道了小黑杨(Populus xiaohei T.S.Hwang ex C.Wang et Tung)细胞培养及植株再生过程,包括花粉愈伤组织的诱导、继代培养、单细胞分离及培养、愈伤组织的再形成及植株再生。试验结果表明:①悬浮细胞的质量是影响细胞培养的根本因子,它主要决定于愈伤组织的质量和振荡培养时间。小黑杨三种类型愈伤组织中只有类型Ⅱ,即淡黄色,表面干燥、疏松的颗粒状愈伤组织适于制备悬浮细胞液,振荡时间一般3—5d。②用液体—固体双层培养法进行小黑杨细胞培养是一成功的尝试。MS培养基大量元素浓度、2,4-D浓度、培养基的pH值是影响细胞分裂生长的重要因子。最佳培养基为附加2,4-D 2mg/L,KT 2mg/L和2％蔗糖的改良MS培养基(大量元素减半)。③细胞培养再形成的愈伤组织转到分化培养基后能整齐一致地转变成类型Ⅰ愈伤组织,分化率和生根率均达到100％。展开更多
基金the National "948" Plan Project in China (Grant No.2006-4-72)
文摘Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L^-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L^-1 2,4-D. The best subculture medium was MS ＋ 0.8 mg＇L-1 2,4-D ＋ 30 g·L^-1 sucrose with a subculture cycle of seven days.
基金Supported by General Project of National Natural Science Foundation of China(41371470)Key Program of Natural Science Foundation of Hubei Province(2011CDA037)Science and Technology Planning Project of Huangshi City(2010A1019-2)
文摘Ricinus communis L. is a new copper hyperaccumulator growing on Tonglushan copper mine in Hubei Province, China. This study aimed to establish a suspension cell line of R. communis L. with stable and rapid growth for further screening of heavy metal-resistant R. communis L. cells and breeding of hyperaccu- mulators. In this study, cell suspension culture conditions were optimized by using orthogonal experimental design with previously induced R. communis L. embyre- genic calluses as experimental materials, to establish the suspension cell line of R. communis L. Under the optimal conditions, growth curves of suspension cells and changes in pH of culture liquid were determined. The results showed that the optimal culture conditions for R. conmmnis L. suspension cell line were ： MS ＋ O. 5 rag/I, 6-BA ＋ O. 2 mg/L NAA ＋ 50 mg/L sucrose ＋ 350 mg/L casein hydrolysate as basic medium, with callus inoculation amount of 2.5 g/50 ml, dark culture at （26 ±2） ℃ with shaking at 110 r/min. Under these conditions, increment of fresh weight and dry weight ofR. commun/s L. suspension cells reached the maximum of 4.56 g/（50 ml 14 d） and 0.49 g/（50 ml 16 d）, respectively. Growth curves of R. communis L. suspension cells were basically in ＂S＂ shape. Each culture cycle lasted 16 d, and the rapid growth stage was from the 6th d to the 14th d. In a culture cycle, pH of the culture liquid declined first and then increased to the maximum and stabilized gradually.
文摘Suspension cultures initiated from callus derived from petiole explants of aspen hybrid （Populus tremuloides×P. tremula） produced somatic embryos. Callus was induced on a MS medium supplemented with 5mg·L^-1 2,4-D and 0.05mg·L^-1 zeatin under light conditions. Embryogenic calli were obtained when a subsequent subculture of calli was suspended in the same basal medium with 10mg·L^-1 2,4-D. The highest number of globular embryos were induced from embryogenic calli by cell suspension culture in a MS liquid medium supplemented with 10mg·L^-1 2,4-D. Genotype and 2,4-D concentration were vital to the induction of embryogenic calli producing competent cells. Embryogenic calli for each genotype were heterogeneous. Green calli with gel-like consistency could yield more competent cells than light yellow embryogenic calli. However, some globular embryos broke into slices and some developed abnormally after one month of culture under the same or other hormonal conditions.
文摘本文报道了小黑杨(Populus xiaohei T.S.Hwang ex C.Wang et Tung)细胞培养及植株再生过程,包括花粉愈伤组织的诱导、继代培养、单细胞分离及培养、愈伤组织的再形成及植株再生。试验结果表明:①悬浮细胞的质量是影响细胞培养的根本因子,它主要决定于愈伤组织的质量和振荡培养时间。小黑杨三种类型愈伤组织中只有类型Ⅱ,即淡黄色,表面干燥、疏松的颗粒状愈伤组织适于制备悬浮细胞液,振荡时间一般3—5d。②用液体—固体双层培养法进行小黑杨细胞培养是一成功的尝试。MS培养基大量元素浓度、2,4-D浓度、培养基的pH值是影响细胞分裂生长的重要因子。最佳培养基为附加2,4-D 2mg/L,KT 2mg/L和2％蔗糖的改良MS培养基(大量元素减半)。③细胞培养再形成的愈伤组织转到分化培养基后能整齐一致地转变成类型Ⅰ愈伤组织,分化率和生根率均达到100％。
