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Evaluation of Phytochemical Analysis and Total Phenol Content of Proso Millet and Barnyard Millet
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作者 Deepa Priya Ramadoss Kirubanandan Shanmugam 《Proceedings of Anticancer Research》 2024年第2期13-19,共7页
Whole grains of proso and barnyard millets were sequentially extracted using different solvents(hexane,chloroform,ethyl acetate,and methanol).Phytochemical analysis was performed qualitatively,and the total phenolic c... Whole grains of proso and barnyard millets were sequentially extracted using different solvents(hexane,chloroform,ethyl acetate,and methanol).Phytochemical analysis was performed qualitatively,and the total phenolic content in the extracts of proso and barnyard millets was quantified.Alkaloids and cardiac glycosides were identified in all solvent extracts of both millets.Anthraquinone and glycosides yielded negative results in all solvent extracts of both millets.Among all the solvent extracts,methanol extracts of proso and barnyard millets showed the presence of major compounds such as flavonoids,terpenoids,amino acids,tannins,and phenolics compounds.The maximum amount of phenols was found in methanolic extracts of proso and barnyard millets(0.669±0.003 and 0.625±0.003),followed by the chloroform extract of proso and barnyard millets(0.284±0.002 and 0.257±0.003).The minimum amount of phenolics was found in the acetone extract of proso and barnyard millets.The methanol extract of both millets showed the presence of major compounds with high phenolic content. 展开更多
关键词 proso millet Barnyard millet Sequential solvent extraction Phytochemical analysis Total phenolic content
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Proso millet醇溶蛋白调节小鼠血清胆固醇代谢的作用及其机理
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作者 刘勇 姚惠源 王强 《中国粮油学报》 EI CAS CSCD 北大核心 2007年第1期101-104,共4页
通过比较分别饲喂不同蛋白源(酪蛋白、大豆分离蛋白、Proso m illet醇溶蛋白)饲料的小鼠血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)、甘油三酯(TG)浓度,旨在评价Proso m illet醇溶蛋白对小鼠胆固醇代谢... 通过比较分别饲喂不同蛋白源(酪蛋白、大豆分离蛋白、Proso m illet醇溶蛋白)饲料的小鼠血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)、甘油三酯(TG)浓度,旨在评价Proso m illet醇溶蛋白对小鼠胆固醇代谢的特定作用,并通过体外实验探讨其作用机理。结果显示,以Prosom illet醇溶蛋白(添加1.83%Lys和0.23%Trp)为唯一氮源的小鼠日粮对TC、TG无明显作用,却能显著提高小鼠血清HDL-c浓度(P<0.05),降低小鼠动脉硬化指数(AI)。但在体外其并不能抑制羟甲基戊二酸单酰辅酶A(HMG-CoA)还原酶的活性,即不能直接抑制胆固醇的合成,所以,其对胆固醇代谢的调节作用另有其他途径。 展开更多
关键词 proso millet醇溶蛋白 氨基酸组成 胆固醇调节 HMG—CoA还原酶
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Morphological diversity and correlation analysis of phenotypes and quality traits of proso millet(Panicum miliaceum L.)core collections 被引量:6
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作者 ZHANG Da-zhong Rabia Begum Panhwar +6 位作者 LIU Jia-jia GONG Xiang-wei LIANG Ji-bao LIU Minxuan LU Ping GAO Xiao-li FENG Bai-li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第5期958-969,共12页
Genetic diversity and comprehensive performance are the basis for the discovery and efficient use of proso millet(Panicum miliaceum L.) core collections. In this study, 386 proso millet core collections were used as m... Genetic diversity and comprehensive performance are the basis for the discovery and efficient use of proso millet(Panicum miliaceum L.) core collections. In this study, 386 proso millet core collections were used as materials to observe inflorescence color, leaf phase, inflorescence density, axis shape, branched spike length, panicle type, trichome, measured area of the top3 leaves, and chlorophyll content of the top3 leaves at filling stage. These core collections were also used to record growth period, plant height, diameter of main stem, plant tiller number, branch number, panicle length, panicle number per plant, and panicle weight per plant at the maturation stage. Starch, fat, protein, and yellow pigment contents in the grain and 1 000-seed weight were also measured after harvest. Then, quantitative traits were used for diversity analysis and comprehensive evaluation of each collection. Correlations between all traits were also analyzed. Results showed that among the 8 quality traits, the Shannon index(H′) of hull color was the highest(1.588) followed by the H′ of inflorescence density(0.984). However, inflorescence color and axis shape were lower. The H′ of 16 quantitative traits were significantly higher than the quality traits with the following traits having the highest indices: fat content(2.092), 1 000-seed weight(2.073), top3 leaves area(2.070), main stem diameter(2.056), and plant height(2.052). Furthermore, all other traits had a diversity higher than 1.900. After a comprehensive evaluation of phenotypic traits, plant height, diameter of main stem, plant tiller number, leaf area of top3 leaves, and 1 000-seed weight were the biggest contributors to the principal components. Six high-fat and high-protein cultivars, including Nuoshu, A75-2, Zhiduoaosizhi, Panlonghuangmi, Xiaobaishu, and Xiaohongshu were also screened. Correlations between the quantitative traits were significant, including the correlation between quality traits and quantitative traits. In conclusion, the core collections can be used as basis for discriminating among proso millet cultivars based on related traits and for further studies on millet with rich genetic diversity, good representation, and significant collection between traits. 展开更多
关键词 proso MILLET PHENOTYPIC TRAITS quality DIVERSITY correlation evaluation
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Yielding Ability and Stability and Adaptability of Proso Millet Varieties 被引量:1
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作者 Yanmiao JIANG Jinying XIANG +4 位作者 Haiquan LI Ming DONG Lingling GENG Xuehai ZHU Guoqing LIU 《Asian Agricultural Research》 2018年第5期82-84,共3页
The yielding ability and stability and adaptability of eight proso millet varieties from Shanxi,Inner Mongolia,Heilongjiang and Hebei were analyzed and evaluated in different ecological regions including Zhangjiakou,H... The yielding ability and stability and adaptability of eight proso millet varieties from Shanxi,Inner Mongolia,Heilongjiang and Hebei were analyzed and evaluated in different ecological regions including Zhangjiakou,Huanghua and Shijiazhuang in 2015. The results showed that Chishu No. 2 and Jishu No. 2 have stronger yielding ability,and their grain yields were 3 144. 42( highest) and 3 133. 15 kg/ha respectively,ranking first and second in the test varieties. In areas with low fertility,especially in saline-alkali areas,they still have potential for high yields,so they are suitable for promotion in large areas in Hebei Province. Neimi No. 8 and Yanshu No. 11 showed special adaptability under different ecological conditions. They require high-fertility soil and can be planted in the spring millet area in north of Hebei. 展开更多
关键词 proso millet Yielding ability ADAPTABILITY YIELD
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Application Effects of Several Herbicides in Spring Proso Millet Area in Northern Hebei Province
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作者 Xiang Jinying Li Haiquan +4 位作者 Jiang Yanmiao Zhu Xuehai Dong Ming Geng Lingling Liu Guoqing 《Plant Diseases and Pests》 CAS 2017年第5期33-36,共4页
According to the ecological environment and current status of weeds in northern Hebei Province,five herbicides were screened out to test weed control efficacy and crop safety. The result showed that Gaolaotou was more... According to the ecological environment and current status of weeds in northern Hebei Province,five herbicides were screened out to test weed control efficacy and crop safety. The result showed that Gaolaotou was more economic and effective when spraying in soil on the sowing day at the dose of 2 250 m L/hm^2. 展开更多
关键词 HERBICIDES proso millet WEED Control effect
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GGE biplot analysis of yield stability and test location representativeness in proso millet (Panicum miliaceum L.) genotypes 被引量:13
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作者 ZHANG Pan-pan SONG Hui +8 位作者 KE Xi-wang JIN Xi-jun YIN Li-hua LIU Yang QU Yang SU Wang FENG Nai-jie ZHENG Dian-feng FENG Bai-li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第6期1218-1227,共10页
The experiments were conducted for three consecutive years across 14 locations using 9 non-waxy proso millet genotypes and 16 locations using 7 waxy proso millet genotypes in China. The objectives of this study were t... The experiments were conducted for three consecutive years across 14 locations using 9 non-waxy proso millet genotypes and 16 locations using 7 waxy proso millet genotypes in China. The objectives of this study were to analyze yield stability and adaptability of proso millets and to evaluate the discrimination and representativeness of locations by analysis of variance(ANOVA) and genotype and genotype by environment interaction(GGE) biplot methods. Grain yields of proso millet genotypes were significantly influenced by environment(E), genotype(G) and their interaction(G×E)(P<0.1%). G×E interaction effect was six times higher than G effect in non-waxy group and seven times in waxy group. N04-339 in non-waxy and Neimi 6(NM6) in waxy showed higher grain yields and stability compared with other genotypes. Also, Neimi 9(NM9, a non-waxy cultivar) and 90322-2-33(a waxy cultivar) showed higher adaptability in 7 and in 11 locations, respectively. For non-waxy, Dalat, Inner Mongolia(E2) and Wuzhai, Shanxi(E5) were the best sites among all the locations for maximizing the variance among candidate cultivars, and Yanchi, Ningxia(E10) had the best representativeness. Wuzhai, Shanxi(e9) and Yanchi, Ningxia(e14) were the best representative locations, and Baicheng, Jilin(e2) was better discriminating location than others for waxy genotypes. Based on our results, E10 and e14 have enhanced efficiency and accuracy for non-waxy genotypes and waxy genotypes selection, respectively in national regional test of proso millet varieties. 展开更多
关键词 产量稳定性 GGE双标图 基因型 位置 糜子 品种区域试验 测试 相互作用
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Diversity and trait-specific sources for productivity and nutritional traits in the global proso millet(Panicum miliaceum L.) germplasm collection 被引量:9
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作者 Mani Vetriventhan Hari D.Upadhyaya 《The Crop Journal》 SCIE CAS CSCD 2018年第5期451-463,共13页
Proso millet is an important short-duration crop that adapts well to varied climatic conditions and is grown worldwide for food,feed and fodder purposes.Owing to a lack of genetic improvement,the crop has experienced ... Proso millet is an important short-duration crop that adapts well to varied climatic conditions and is grown worldwide for food,feed and fodder purposes.Owing to a lack of genetic improvement,the crop has experienced no yield improvement and provides low income to farmers.In this study,200 accessions of proso millet originating in 30 countries were evaluated in two rainy seasons to assess phenotypic diversity for morpho-agronomic and grain nutritional traits and to identify high grain-yielding and grain nutrient-rich accessions.Proso millet diversity was structured by geographical region,by country within region,and by racial group.Race patentissimum showed high diversity and ovatum low diversity,and diverged widely from each other.The lowest divergence was observed between races compactum and ovatum.Eighteen high grain-yielding,10 large-seeded,and 26 two or more grain nutrients-rich accessions were identified,and highly diverse pairs of accessions within and between trait groups were identified.They included IPm 9 and IPm2661 for high grain yield and large seed size;and IPm 2069,IPm 2076,and IPm 2537 for high Fe,Zn,Ca,and protein contents.IPm 2875 had a seed coat that is readily removed by threshing.This study provides valuable information to proso millet researchers about agronomic and nutritional traits in accessions that could be tested for regional adaption and yield for direct release as cultivars,and could be used in breeding for developing high grain-yielding and nutrient-rich cultivars. 展开更多
关键词 short-duration morpho-agronomic
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Diversity Evaluation of Proso Millet Germplasm Resources in Dryland
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作者 Xiaojuan ZHANG Bingwen CHENG +4 位作者 Yong WANG Junxue YANG Shangpei ZHANG Shiwu LUO Xiaojun WANG 《Agricultural Biotechnology》 CAS 2018年第5期71-75,共5页
[Objective] This study was to provide a theoretical basis for the selection of the parents of proso millet breeding in dry land of south Ningxia,with the aim to improve the production of hilly proso millet in Ningxia ... [Objective] This study was to provide a theoretical basis for the selection of the parents of proso millet breeding in dry land of south Ningxia,with the aim to improve the production of hilly proso millet in Ningxia hilly area. [Method]The genetic diversity of 46 accessions of dryland proso millet germplasm at 11 agronomic traits were analyzed by using principal component analysis,Shannon-weaver diversity index and non-weighted matching arithmetic average clustering method( UPGMA). Cluster analysis was also performed. [Result] The proso millet resources mainly had green inflorescence color,panicle spikes,and yellow grain color. The maximum diversity index of proso millet was 2. 03. The variation coefficient of grain weight per plant was 19. 23. In all the principal components,the main information concentrated on the first 4 principal components with the cumulative contribution rate reaching 83. 34%. The tested materials were clustered into 3 categories at euclidean distance of 0. 984 4. The first category contained 41 germplasm resources,the second contained 2,and the third category contained 3.[Conclusion]This study could provide references for the targeted use of drought-resistant parents to select hybrid combinations of proso millet in Ningxia. 展开更多
关键词 黍稷 资源 欧几里德几何学 评估 主要部件 聚类方法 累积贡献率 基因差异
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糜子淀粉结构及理化特性研究进展
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作者 李梦琴 田争争 +2 位作者 田潇凌 常逍柯 林顺顺 《河南农业大学学报》 CAS CSCD 北大核心 2024年第1期35-43,共9页
由于糜子营养丰富且升糖指数低,糜子资源的开发受到越来越多的关注。现有研究表明,淀粉作为糜子的主要成分,其结构对理化特性和糜子制品的品质有极大的影响。本研究从颗粒形貌、分子结构、结晶特性3个方面对糜子淀粉的多尺度结构进行综... 由于糜子营养丰富且升糖指数低,糜子资源的开发受到越来越多的关注。现有研究表明,淀粉作为糜子的主要成分,其结构对理化特性和糜子制品的品质有极大的影响。本研究从颗粒形貌、分子结构、结晶特性3个方面对糜子淀粉的多尺度结构进行综述,并从糊化特性、热焓特性、流变特性和消化特性4个方面对糜子淀粉的理化特性进行分析,对比了粳性糜子淀粉和糯性糜子淀粉多尺度结构及理化特性的差别,探讨了糜子淀粉的结构特征与理化特性之间的关系,同时指出对糜子淀粉多尺度结构和理化特性之间关系的研究仍有不足,缺乏对不同品种糜子淀粉间多尺度结构和理化特性的系统比较等问题,以期为糜子资源的开发利用提供基础理论参考。 展开更多
关键词 糜子 淀粉 多尺度结构 理化特性
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糜子改性淀粉的理化性质及体外消化特性研究
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作者 王鑫 李丹 +4 位作者 王华 李秀萍 杨丽红 张晔 王浩 《中国食品添加剂》 CAS 2024年第3期147-154,共8页
本研究将脱支糜子淀粉(DPM)与不同碳链长度(12〜18)的饱和脂肪酸复合,制备糜子改性淀粉(DPM-FA),并测定其理化性质和体外消化特性。研究发现,糜子改性淀粉的复合指数与脂肪酸碳链长度密切相关,且随着脂肪酸碳链长度的增加,复合指数呈现... 本研究将脱支糜子淀粉(DPM)与不同碳链长度(12〜18)的饱和脂肪酸复合,制备糜子改性淀粉(DPM-FA),并测定其理化性质和体外消化特性。研究发现,糜子改性淀粉的复合指数与脂肪酸碳链长度密切相关,且随着脂肪酸碳链长度的增加,复合指数呈现先增高后降低的趋势,最高至23.09%。天然糜子淀粉(PMS),脱支糜子淀粉以及糜子改性淀粉的晶型有所变化,由A型转变为B型进而变为V型。随着晶型的改变,糜子改性淀粉热稳定性最高,焓变(ΔH)最高至5.26 J/g,且其体外消化率最低,相比天然糜子淀粉降低14.65%,这与抗性淀粉含量增加10.31%,快消化淀粉含量降低10.35%密切相关。综上,糜子改性淀粉的高稳定和低消化性的特质,使其有望作为降血糖功能性食品的重要来源。 展开更多
关键词 糜子淀粉 脂肪酸 淀粉-脂肪酸复合物 结构表征 体外消化特性
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糜子核心种质成株期抗旱性鉴定评价与抗旱种质筛选 被引量:1
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作者 王倩 董孔军 +7 位作者 薛亚鹏 刘少雄 王若楠 杨佳琪 陆平 王瑞云 杨天育 刘敏轩 《中国农业科学》 CAS CSCD 北大核心 2023年第21期4163-4174,I0016-I0025,共22页
【目的】干旱是影响糜子生长发育及产量的主要制约因素之一,筛选优异抗旱资源及鉴定指标,为抗旱品种的选育和抗旱分子机制的研究奠定基础。【方法】以200份糜子核心种质资源为研究对象,于2021—2022年在甘肃敦煌连续开展2年的田间成株... 【目的】干旱是影响糜子生长发育及产量的主要制约因素之一,筛选优异抗旱资源及鉴定指标,为抗旱品种的选育和抗旱分子机制的研究奠定基础。【方法】以200份糜子核心种质资源为研究对象,于2021—2022年在甘肃敦煌连续开展2年的田间成株期抗旱性鉴定,试验设置正常灌水和干旱胁迫2个处理,测定叶面积(leafarea,LA)、主茎直径(mainstem diameter,MSD)、主茎节数(number of main stem nodes,NMSN)、主穗长(main panicle length,MPL)、穗下节间长度(pedunclelength,PL)、株高(plantheight,PH)、单株草重(strawweightperplant,SWPP)、单株穗重(panicleweight perplant,PWPP)、单株粒重(grainweightperplant,GWPP)、千粒重(thousandgrainweight,TGW)和小区产量(yield perplot,YPP)11个指标,采用综合抗旱系数(CDTC值)、抗旱指数(DRI值)和抗旱性度量值(D值)对糜子成株期抗旱性进行综合评价。【结果】在不同水分处理条件下,叶面积、主茎直径、主茎节数、主穗长、穗下节间长度、株高、单株草重、单株穗重、单株粒重、千粒重和小区产量11个指标在不同种质间均表现为显著差异。干旱胁迫处理下,糜子的生长受到抑制,11个指标测定值均较正常灌水处理明显降低,且小区产量对干旱胁迫较敏感。相关性分析发现,11个测定指标的抗旱系数间均呈现出一定的相关性,其中,单株穗重和单株粒重的相关性最强,相关系数为0.943。主成分分析将11个鉴定指标转换成6个主成分,累计方差贡献率达80.667%。基于CDTC值、DRI值和D值的糜子资源抗旱性排序基本一致。根据D值利用聚类分析将200份糜子种质资源抗旱性划分为4类,第Ⅰ类为强抗旱型有10份,第Ⅱ类为抗旱型有70份,第Ⅲ类为干旱敏感型有81份,第Ⅳ类为干旱极敏感型有39份。株高、单株粒重、单株穗重和主穗长与D值的相关性较高,相关系数分别为0.756、0.697、0.696和0.679。通过逐步回归分析构建了糜子成株期抗旱评价的回归方程:Y=-1.509+0.362X1+0.174X2+0.349X3+0.389X4+0.307X5+0.251X6+0.218X7。【结论】抗旱性度量值法适宜于糜子成株期抗旱性评价;筛选出巴林左疙塔黍(00000525)、高台乌糜子(00002677)和民乐红糜子(00002687)等10份强抗旱性材料;株高、单株穗重和主穗长可作为糜子成株期抗旱性鉴定的主要指标。 展开更多
关键词 糜子 大田成株期 抗旱鉴定 抗旱种质筛选
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Physicochemical and structural properties of starches from non-traditional sources in China 被引量:1
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作者 Jingyi Yang Chagam Koteswara Reddy +1 位作者 Zhili Fan Baojun Xu 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期416-423,共8页
In this study,we isolated starches from non-traditional sources,including quinoa,lentil,arrowhead,gorgon fruit,sorghum,chickpea,proso millet,and purple potato and investigated their morphology,physicochemical,and func... In this study,we isolated starches from non-traditional sources,including quinoa,lentil,arrowhead,gorgon fruit,sorghum,chickpea,proso millet,and purple potato and investigated their morphology,physicochemical,and functional properties.Significant differences in starch particle morphology,swelling power,solubility,syneresis,crystalline pattern,and pasting viscosity were observed among the starches from these nontraditional sources.Further,all these isolated starches had unique properties because of their characteristic distinct granules when seen under scanning electron microscopy(SEM).The amylose content of the isolated starches shown significant difference(P<0.05),and the values ranged between 11.46%and 37.61%.Results demonstrated that the isolated starches contained between 79.82%to 86.56%starch,indicating that the isolated starches had high purity.X-ray diffraction(XRD)patterns of starches isolated from sorghum,proso millet,quinoa,purple potato,and gorgon fruit presented A-type diffraction pattern;while lentil seeds,arrowhead,and chickpea starches presented C-type diffraction pattern.Overall,these results will promote the development of products based on starch isolated from non-traditional starches. 展开更多
关键词 STARCH VISCOSITY Morphology QUINOA SORGHUM proso millet
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糜子黄、黑果皮遗传及转录组学分析
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作者 李强 白璐 +1 位作者 郭世华 高志军 《西北植物学报》 CAS CSCD 北大核心 2023年第8期1268-1275,共8页
为了解糜子黑色与黄色果皮的遗传规律以及其形成机理,利用黄色果皮品种‘黄粒糜Ⅰ’和黑色果皮品种‘2016106Ⅰ’构建杂种F1、F2代,分析果皮颜色的遗传规律,利用F3代籽粒进行转录组测序,挖掘影响糜子籽粒颜色的关键基因。试验结果表明,... 为了解糜子黑色与黄色果皮的遗传规律以及其形成机理,利用黄色果皮品种‘黄粒糜Ⅰ’和黑色果皮品种‘2016106Ⅰ’构建杂种F1、F2代,分析果皮颜色的遗传规律,利用F3代籽粒进行转录组测序,挖掘影响糜子籽粒颜色的关键基因。试验结果表明,黑色对黄色为显性,由单基因控制;根据GO和KEGG富集分析结果,共筛选出13个与类黄酮合成途径相关的差异基因,仅有C2845_PM02G08740、C2845_PM04G29280和C2845_PM09G22680为上调表达基因,其余10个均为下调表达基因,上述基因编码肉桂醇脱氢酶和肉桂酰CoA还原酶等多种酶,与颜色的形成密切相关。 展开更多
关键词 糜子 转录组测序 颜色 差异表达基因
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黍稷高基元EST-SSR标记开发及200份核心种质资源遗传多样性分析 被引量:3
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作者 王倩 张立媛 +6 位作者 许月 李海 刘少雄 薛亚鹏 陆平 王瑞云 刘敏轩 《作物学报》 CAS CSCD 北大核心 2023年第8期2308-2318,共11页
为搞清黍稷核心种质资源的遗传背景,开发新的高基元EST-SSR标记用以评估国内不同生态区资源的遗传多样性,为加快黍稷育种进程和挖掘优异种质提供依据。本研究基于转录组测序开发了200个高基元EST-SSR标记并进行多态性筛选,利用筛选到的... 为搞清黍稷核心种质资源的遗传背景,开发新的高基元EST-SSR标记用以评估国内不同生态区资源的遗传多样性,为加快黍稷育种进程和挖掘优异种质提供依据。本研究基于转录组测序开发了200个高基元EST-SSR标记并进行多态性筛选,利用筛选到的多态性标记评价200份黍稷核心种质资源的遗传多样性。结果表明,200个标记在6份地理来源差异显著的黍稷材料中有52个呈多态性,开发效率为26%,其中四、五和六碱基重复多态性标记分别为17个(32.7%)、17个(32.7%)和18个(34.6%)。利用52个高基元EST-SSR多态性标记对200份核心资源进行检测,共得到129个观测等位变异,每个位点检测到2~3个;Shannon多样性指数为0.3093~1.0910,平均为0.7277;多态性信息含量为0.1948~0.8211,平均为0.5104。基于UPGMA将200份资源划分为6个群组。基于Structure的遗传结构(K=7)将资源划分为7个类群,黍稷资源主要来自4个(东北地区、华北地区、黄土高原和北方地区)基因库。基于主成分分析将材料聚为6个类群,与其地理来源一致。构建了52个四–六碱基重复EST-SSR标记,开发率为26%;用其分析材料的遗传差异发现黍稷地方核心种质遗传多样性较为丰富。 展开更多
关键词 黍稷 核心种质 高基元EST-SSR 遗传多样性 群体结构
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糜子GBSSI基因功能标记的开发与验证 被引量:1
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作者 丁敏 段政勇 +5 位作者 王宇卓 薛亚鹏 王海岗 陈凌 王瑞云 乔治军 《作物学报》 CAS CSCD 北大核心 2023年第3期703-718,共16页
根据糜子胚乳直链淀粉合成调控基因Waxy(GBSSI)上已知功能位点的核苷酸差异设计功能分子标记,并对山西省270份糜子资源进行基因型检测和基因分型。通过对粳糯性状的鉴定,将表型与基因型结合对分子标记进行验证。对46份不同基因型糜子资... 根据糜子胚乳直链淀粉合成调控基因Waxy(GBSSI)上已知功能位点的核苷酸差异设计功能分子标记,并对山西省270份糜子资源进行基因型检测和基因分型。通过对粳糯性状的鉴定,将表型与基因型结合对分子标记进行验证。对46份不同基因型糜子资源进行直链淀粉含量测定,分析该基因的遗传效应,评估其育种利用价值。GBSSI基因在糜子中以2种形式(L和S)存在,针对S型基因15 bp的InDel位点设计了一个功能标记(RYW214),该标记能有效区分126 bp、141 bp和杂合(126/141 bp)3种带型,粳糯性鉴定显示该标记结果与表型鉴定结果吻合度高,达93.30%,Pearson相关性指数r为0.745。针对L型基因上的一个单核苷酸插入位点和一个SNP位点,设计了2个CAPS标记(RYW215、RYW216),该标记可对L基因准确分型。270份资源中,共有11种基因型。其中,基因型S_(-15)/L_(F)最多,有84份(31.10%),其次是S_(0)/S_(-15)/L_(F)(22.96%),S_(-15)/L_(Y)/L_(F)最少(0.74%),未发现S_(-15)/L_(C)。46份材料中,直链淀粉含量较高的基因型为S_(0)/L_(Y)/L_(F)(15.50%),最低为S_(-15)/L_(F)(0.58%)。突变基因型S_(-15)会导致直链淀粉含量出现质的下降,杂合基因型S0S_(-15)直链淀粉含量稍高于纯合基因型S_(-15)。 展开更多
关键词 糜子 GBSSI基因 功能分子标记 直链淀粉含量
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基于电子束辐照处理的粳糯糜子理化特性及抗氧化活性 被引量:1
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作者 杨苗 林姿娜 +3 位作者 王永伦 李兴 雷宏杰 徐怀德 《食品科学》 EI CAS CSCD 北大核心 2023年第17期43-50,共8页
目的:探究电子束辐照对粳糯糜子理化特性及抗氧化活性的影响。方法:采用0(对照)、2、4、6、8、10kGy不同剂量电子束分别辐照‘榆黍1号’(糯)和‘榆糜2号’(粳)两种糜子,测定处理后糜子的理化指标、生物活性物质水平及抗氧化能力。结果表... 目的:探究电子束辐照对粳糯糜子理化特性及抗氧化活性的影响。方法:采用0(对照)、2、4、6、8、10kGy不同剂量电子束分别辐照‘榆黍1号’(糯)和‘榆糜2号’(粳)两种糜子,测定处理后糜子的理化指标、生物活性物质水平及抗氧化能力。结果表明,经电子束辐照处理后,粳糯糜子籽粒物理性质、水分和蛋白质量分数无显著变化(P>0.05),色泽、淀粉质量分数、灰分质量分数、酚类物质含量及抗氧化活性发生显著变化(P<0.05)。此外,电子束辐照处理后的粳糯糜子总酚、总黄酮含量、抗氧化活性及单体酚含量总体明显提高,表现出较强的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除活性(最高分别为78.01%和70.22%)和2,2-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基(最高分别为61.51%和50.57%)清除活性;粳糯糜子提取液中没食子酸、咖啡酸、白藜芦醇、儿茶素和山柰酚含量增加,原儿茶酸和表儿茶素含量较为稳定。相关性分析结果表明,总酚含量与儿茶素、槲皮素含量呈极显著正相关(P<0.01),总黄酮含量与芦丁、没食子酸含量呈极显著正相关(P<0.01),抗氧化活性与儿茶素含量呈正相关。综上,与未经辐照的糜子相比,电子束辐照处理后的糜子具有较高的酚类物质含量和抗氧化性,研究和开发价值巨大。 展开更多
关键词 电子束辐照 糜子 酚类物质 理化特性 抗氧化活性
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糜子直链淀粉、蛋白质含量对其外观品质及食味品质的影响 被引量:3
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作者 李冬梅 王洪露 +3 位作者 龚瑶 曹梓微 李佳雨 冯佰利 《食品科学》 EI CAS CSCD 北大核心 2023年第4期115-121,共7页
以4个直链淀粉、蛋白质含量存在显著差异的糜子品种为实验材料,研究直链淀粉、蛋白质含量对糜子外观及食味品质的影响。结果表明:与低直链淀粉品种比较,高直链淀粉糜子品种,全粉糊化黏度、糊化时间、糊化温度较高,糜子饭吸水率、体积膨... 以4个直链淀粉、蛋白质含量存在显著差异的糜子品种为实验材料,研究直链淀粉、蛋白质含量对糜子外观及食味品质的影响。结果表明:与低直链淀粉品种比较,高直链淀粉糜子品种,全粉糊化黏度、糊化时间、糊化温度较高,糜子饭吸水率、体积膨胀率、硬度较高,而糊化热焓值较低;蛋白质含量较高的糜子品种,全粉呈现低糊化黏度,糜子饭呈现低吸水率、体积膨胀率,高硬度。直链淀粉、蛋白质含量较高的糜子品种,蒸煮性差、能耗高、蒸煮时间长。因此,在糜子品质育种中,适当降低直链淀粉和蛋白质含量可以改善糜子的适口性。 展开更多
关键词 糜子 直链淀粉 蛋白质 外观品质 食味品质
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基于GYT双标图分析对国家糜子区域试验品种的综合评价
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作者 马智秀 柴少华 +6 位作者 郭岩 石兴 杨清华 高金锋 高小丽 冯佰利 杨璞 《中国农业科学》 CAS CSCD 北大核心 2023年第16期3062-3076,I0001-I0004,共19页
【目的】利用GYT双标图分析糜子在不同试点的农艺性状和产量及品种-性状间的互作特性,并对参试品种开展多性状综合评判和分类研究,为品种合理布局和区域规划提供理论依据。【方法】结合相关性分析,利用GGE双标图、GT双标图和GYT双标图... 【目的】利用GYT双标图分析糜子在不同试点的农艺性状和产量及品种-性状间的互作特性,并对参试品种开展多性状综合评判和分类研究,为品种合理布局和区域规划提供理论依据。【方法】结合相关性分析,利用GGE双标图、GT双标图和GYT双标图技术对2019—2020年国家糜子区域试验20个参试品种在15个试点的生育天数、株高、主茎节数、主穗长、穗粒重、千粒重等农艺性状和产量进行综合评价。【结果】相关性分析表明,在粳性和糯性糜子中,产量都与生育天数极显著正相关,与主穗长极显著负相关,株高与生育天数和主穗长显著正相关。在粳性糜子中,产量与主茎节数显著负相关,主茎节数和千粒重极显著正相关。在糯性糜子中,产量与千粒重极显著正相关,生育天数与株高和千粒重显著正相关。用GT双标图对糜子参试品种基因型-性状的互作效应分析表明,粳性糜子和糯性糜子GT双标图的主成分PC1和PC2分别解释了基因型-性状互作效应的61.81%和69.96%,GT双标图展示的参试品种的农艺性状间相关性与参试品种性状间皮尔森相关性系数基本一致。运用GYT双标图技术对参试品种的产量-性状组合间两两相关性进行分析,所有组合性状均显著正相关。计算各品种的理想指数,以此为标准鉴定出伊11-02-92-4、固19-63、0515-2-2、伊11-03-3-2-2和正隆糜1号等品种为产量-性状组合表现优良的品种,赤糜3号、西农2018-N02、西农2018-N10、Y1660、西农18-W02和西农18-W06等品种综合表现较差。利用GGE双标图对参试品种产量的丰产性和稳定性及适宜生态区分析,结果表明,伊11-02-92-4和0515-2-2在不同种植区较其他品种具有更广泛的适应性和更好的丰产性,展现出绝对的区域产量优势。【结论】糜子品种多性状评价方法中,GYT双标图分析评价的糜子品种结果相较于GGE和GT双标图可信度更高,是科学评价糜子品种优劣的有效方法。国家糜子区域试验参试品种中,综合表现较好的粳性品种为伊11-02-92-4,该品种适宜在东北春糜子区和黄土高原春夏糜子区推广种植;综合表现较好的糯性品种为0515-2-2,该品种适宜在北方春糜子区和黄土高原春夏糜子区推广种植。 展开更多
关键词 GYT双标图 理想指数 糜子 区域试验 产量-性状组合
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复合酶法大黄米多孔淀粉的制备及其微观结构和理化性质 被引量:1
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作者 秦智欣 郑明珠 +2 位作者 林楠 潘楚 刘景圣 《中国食品学报》 EI CAS CSCD 北大核心 2023年第5期138-150,共13页
以吸油率为指标,大黄米淀粉为原料,采用复合酶法(α-淀粉酶和糖化酶)制备大黄米多孔淀粉。通过单因素实验与Box-Benhnken响应面试验优化大黄米多孔淀粉制备的工艺参数,并对原淀粉及大黄米多孔淀粉进行结构表征。结果表明,复合酶法制备... 以吸油率为指标,大黄米淀粉为原料,采用复合酶法(α-淀粉酶和糖化酶)制备大黄米多孔淀粉。通过单因素实验与Box-Benhnken响应面试验优化大黄米多孔淀粉制备的工艺参数,并对原淀粉及大黄米多孔淀粉进行结构表征。结果表明,复合酶法制备大黄米多孔淀粉的最优工艺参数是:复合酶添加量1.2%,酶解温度56℃,酶解时间14 h,酶解pH 4.6,复合酶配比1∶4。在此条件下大黄米多孔淀粉的吸油率为(174.00±2.00)%。扫描电镜结果显示,形成多孔淀粉后,淀粉颗粒表面存在不均匀分布的孔洞及圆形凹陷,内部呈中空结构。粒度分布测试结果显示,多孔淀粉粒径均减小,淀粉颗粒分布均一度提高。X-射线衍射分析及傅里叶红外光谱表明,酶的水解作用不会改变大黄米淀粉的A型晶体结构及基本化学结构,相对结晶度和红外吸收峰均明显增加,淀粉颗粒内部有序程度提高。低温氮气吸附结果表明,复合酶酶解作用使大黄米淀粉的比表面积由13.9 m^(2)/g增加至29.42 m^(2)/g,孔径由4.143 nm增加至6.637 nm,孔容由14.81×10-3 cm3/g增加至25.05×10-3 cm3/g。黏度测定结果表明,多孔淀粉峰值黏度、崩解值降低。本研究可为多孔淀粉的开发应用及大黄米的精深加工提供参考。 展开更多
关键词 大黄米淀粉 多孔淀粉 吸油率 糖化酶 Α-淀粉酶
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建声电声综合设计STIPA探讨——广西省游泳跳水馆的声系统工程介绍 被引量:1
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作者 燕翔 王鹏 +1 位作者 窦文华 顾科 《电声技术》 2014年第4期6-12,共7页
介绍了广西省游泳跳水馆的工程概况,详细介绍了游泳跳水馆STIPA指标的设计过程,并对实施后的第三方检测指标进行了对比,分析了空场条件下观众区STIPA指标的完成情况,最后对游泳跳水馆工程进行了总结。工程经过清华大学建筑物理实验室的... 介绍了广西省游泳跳水馆的工程概况,详细介绍了游泳跳水馆STIPA指标的设计过程,并对实施后的第三方检测指标进行了对比,分析了空场条件下观众区STIPA指标的完成情况,最后对游泳跳水馆工程进行了总结。工程经过清华大学建筑物理实验室的咨询指导,中建八局的精心施工、调试,最终实现了在空场RT60为7 s条件下的STIPA实现了0.50的任务目标。扩声系统顺利通过了第三方检测的工程验收,扩声工程验收指标达到和超过国家标准GB/T 28049—2011的体育馆扩声一级指标。 展开更多
关键词 建声电声综合设计 STIPA 指向性Q值 proso线源阵列扬声器 声场干涉
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