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Chemical tags and beyond:Live-cell protein labeling technologies for modern optical imaging
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作者 Dilizhatai Saimi Zhixing Chen 《Smart Molecules》 2023年第2期16-29,共14页
Imaging proteins with high resolution is crucial for studying cellular physiology and pathology.Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells.In recent year... Imaging proteins with high resolution is crucial for studying cellular physiology and pathology.Fluorescence imaging is a privileged method to visualize proteins with subcellular precision in live cells.In recent years,there has been a tremendous advance in the field of fluorescent dyes that are optically more sophisticated than genetically-encodable fluorescent proteins.In this review,we aim to discuss modern bioconjugation methods to specifically incorporate these dyes into protein-of-interests.We focus on advances in live-cell labeling strategies and fluorescent probes,especially the HaloTag,SNAP-tag,TMP-tag,and unnatural amino acid systems and their applications.These protein labeling methods,along with cutting-edge dyes and novel microscopy methods,have become the infrastructure for biological research in the era of super-resolution imaging. 展开更多
关键词 fluorescent dyes live-cell imaging protein labeling self-labeling tags unnatural amino acids
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A versatile AIE fluorogen with selective reactivity to primary amines for monitoring amination,protein labeling,and mitochondrial staining
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作者 Xinyuan He Huilin Xie +14 位作者 Lianrui Hu Pengchao Liu Changhuo Xu Wei He Wutong Du Siwei Zhang Hao Xing Xinyue Liu Hojeong Park Tsz Shing Cheung Min-Hui Li Ryan T.K.Kwok Jacky W.Y.Lam Jian Lu Ben Zhong Tang 《Aggregate》 2023年第1期250-259,共10页
Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research.Despite all the efforts,scientists lack a proper strategy to achieve high selectivity for primary amines,... Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research.Despite all the efforts,scientists lack a proper strategy to achieve high selectivity for primary amines,not to mention the requirement of fast response in real applications.Herein,we report a chromone-based aggregation-induced emission(AIE)fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification,and its applications for monitoring bioprocesses of amination and protein labeling.CMVMN is AIE-active and capable of solid-state sensing.Thus,its electrospun films are manufactured for visualization of amine diffusion and leakage process.CMVMN also shows good biocompatibility and potential mitochondria-staining ability,which provides new insight for organelle-staining probe design.Combined with its facile synthesis and good reversibility,CMVMN would not only show wide potential applications in biology,but also offer new possibilities for molecular engineering. 展开更多
关键词 aggregation-induced emission(AIE) BIOCONJUGATION mitochondria-staining primary amines protein labeling
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SNAP-tag fluorogenic probes for wash free protein labeling
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作者 Shuang Leng Qing-Long Qiao +3 位作者 Yue Gao Lu Miao Wu-Guo Deng Zhao-Chao Xu 《Chinese Chemical Letters》 SCIE CAS CSCD 2017年第10期1911-1915,共5页
Protein labeling by using a protein tag and tag-specific fluorescent probes is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. SNAP-tag as one of the most prominent fusi... Protein labeling by using a protein tag and tag-specific fluorescent probes is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. SNAP-tag as one of the most prominent fusion tags has been widely used and already commercially available. Recently, various fluorogenic probes for SNAP-tag based protein labeling were reported. Owing to turn-on fluorescence response, fluorogenic probes for SNAP-tag minimize the fluorescence background caused by unreacted or nonspecifically bound probes and allow for direct imaging in living cells without wash-out steps. Thus,real-time analysis of protein localization, dynamics and interactions has been made possible by SNAP-tag fluorogenic probes. In this review,we describe the design strategies of fluorogenic probes for SNAP-tag and their applications in cellular protein labeling. 展开更多
关键词 Fluorogenic probes Wash free SNAP-TAG protein labeling
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LABELING OF PROTEIN WITH A NEW EUROPIUM CHELATE OF 5-CHLOROSULFOYL-2-THENOYLTRIFLUOROACETONE(CTTA)AND APPLICATION TO IMMUNOASSAY 被引量:1
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作者 Yun Xiang CI Xiao Da YANG Chemistry Department,Peking University,Beijing 100871 《Chinese Chemical Letters》 SCIE CAS CSCD 1992年第12期1007-1010,共4页
We describe for the first time the synthesis and the optimal conditions for protein labeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms a highly fluorescent conplex with E... We describe for the first time the synthesis and the optimal conditions for protein labeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms a highly fluorescent conplex with Eu^(3+) when conjugated to protein.The labeled proteins were characterized by absorbance and fluorescence measurements and the effect of labeling on the biological activity of sone proteins was also studied.It is shown that the new label is suitable for applications in time-resolved fluoroimmunoassay. 展开更多
关键词 Eu CTTA)AND APPLICATION TO IMMUNOASSAY labeling OF protein WITH A NEW EUROPIUM CHELATE OF 5-CHLOROSULFOYL-2-THENOYLTRIFLUOROACETONE HSA
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Construction of bicistronic green fluorescent protein labeled pSELECT GFPzeo human bone morphogenetic protein 2 eukaryotic expression vector
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作者 黄洪超 《外科研究与新技术》 2011年第2期91-91,共1页
Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by... Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by using polymerase 展开更多
关键词 PCR GFP Construction of bicistronic green fluorescent protein labeled pSELECT GFPzeo human bone morphogenetic protein 2 eukaryotic expression vector GENE
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Electron-withdrawing inductive effects enhanced strategy for protein thiol sensing and blocking agent design
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作者 Liangwei Zhang Shudi Liu +2 位作者 Xia Zhang Jinyu Sun Lingxin Chen 《Smart Molecules》 2024年第1期118-123,共6页
It is a great challenge to discover novel chemical reactions suitable for biological analysis in a living system.The development of novel protein thiol blocking agents is a crucial need for exploring protein thiol fun... It is a great challenge to discover novel chemical reactions suitable for biological analysis in a living system.The development of novel protein thiol blocking agents is a crucial need for exploring protein thiol functions in protein refolding,signal transduction,and redox regulation.We are always keen on seeking novel chemical reactions applied to endogenous biological macromolecules or protein thiol sensing,blocking,and labeling.In the present work,we have successfully developed a novel agent to block protein thiol by enhanced electron-withdrawing inductive effects.This sensing and blocking process was detailedly monitored by UV-vis,fluorescent spectra,and SDS-Page gel separation.The spectral studies demonstrated that the agent could react ultrafastly with thiol within seconds atμM level.Furthermore,fluorescent imaging in cells and in vivo was further used for the validation of its ability to sensing and blocking thiol,providing evidence of downregulated protein thiols in Parkinson's disease.The enhanced electronwithdrawing inductive effect strategy in this work may provide a general guideline for designing protein thiol agent. 展开更多
关键词 bio-thiol fluorescent sensing Parkinson's disease protein labeling sulfone
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A mini-review on the enzyme-mediated manipulation of proteins/peptides
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作者 Shaomin Lin Chunmao He 《Chinese Chemical Letters》 SCIE CAS CSCD 2018年第7期1017-1021,共5页
Recent years have seen an ever increasing number of enzyme mediated protein/peptide modification reactions, which contribute significantly to the elucidation of related biological functions. The many available enzymes... Recent years have seen an ever increasing number of enzyme mediated protein/peptide modification reactions, which contribute significantly to the elucidation of related biological functions. The many available enzymes have, however, caused difficulties for practitioners in choosing the most appropriate enzyme for a certain purpose. This review surveyed the widely used enzymes(i.e., sortases, butelase 1,subtiligase, formylglycine generating enzyme and farnesyltransferase) in the manipulation of proteins/peptides, and the application fields of these enzymes as well as the advantages and limitations of each enzyme are summarized. 展开更多
关键词 protein modification protein labeling protein ligation Site-selective Enzyme-mediated
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Emerging markers for antimicrobial resistance monitoring 被引量:1
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作者 Zineng Yi Xiaona Xu +4 位作者 Xiaohan Meng Congyu Liu Qianpeng Zhou Deyan Gong Zhengbao Zha 《Chinese Chemical Letters》 SCIE CAS CSCD 2023年第10期55-66,共12页
The appearance and spread of antibiotic-resistant pathogens known as antimicrobial resistance(AMR)is one of the major worldwide health crises that humanity have to deal with over the next decades.One of the main metho... The appearance and spread of antibiotic-resistant pathogens known as antimicrobial resistance(AMR)is one of the major worldwide health crises that humanity have to deal with over the next decades.One of the main methods for addressing AMR is the effective screening for antimicrobial insensitivity in clinical and environmental monitoring.Current clinical laboratory procedures use traditional culturebased antibiotic susceptibility testing(AST)methods,which can take up to 24 h to identify which drug is suitable for the infection inhibition.Therefore,it is vital to develop novel strategies that offer quick,simple,affordable,reliable,sensitive and accurate AMR monitoring.Sensors for AMR markers detection could possess the essential qualities for quickly identifying resistant microorganisms and could give vital data for the selection of antibacterial drugs administration.This review offers a summary of the innovative application of these AMR markers detection strategies focusing on healthcare and environmental surveillance for the AMR genotypic or phenotypic assessment. 展开更多
关键词 Antimicrobial resistance markers Genotypic determination Enzymes detection Antibody labeling protein labeling
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