Objective: To investigate the effects of the leaf ethanolic extract of Pseuderanthemum palatiferum(PPE) and its isolated phytochemicals, stigmasterol and sitosterol-3-O-b-Dglucopyranoside, against a-amylase and a-gluc...Objective: To investigate the effects of the leaf ethanolic extract of Pseuderanthemum palatiferum(PPE) and its isolated phytochemicals, stigmasterol and sitosterol-3-O-b-Dglucopyranoside, against a-amylase and a-glucosidase enzyme activities both in vitro and in vivo.Methods: A concentration of maltose, which is a product released in a-amylase-catalyzing reaction, was used as an index of in vitro a-amylase activity. Meanwhile, in vitro a-glucosidase enzyme activity was indicated by the amount of liberated p-nitrophenol in a-glucosidase-catalyzing reaction. In vivo a-amylase and a-glucosidase enzyme activities were evaluated in the normal rats by using oral starch tolerance test and oral sucrose tolerance test, respectively.Results: PPE exerted a concentration-dependent inhibitory action against both aamylase and a-glucosidase in vitro with the IC_(50) values of(11.79 ± 8.10) mg/m L and(1.00 ± 0.11) mg/m L, respectively. Stigmasterol and sitosterol-3-O-b-D-glucopyranoside also exerted an in vitro a-amylase inhibition with the IC_(50) values of(59.41 ± 8.22) mg/m L and(111.19 ± 9.02) mg/m L, respectively. However, these phytochemicals did not produce a concentration-dependent inhibition against in vitro a-glucosidase activity. PPE and its isolated phytochemicals significantly decreased the blood glucose levels at t = 30 min in the oral starch tolerance test. From the sucrose tolerance test, only PPE but not its isolated phytochemicals significantly caused a depletion in the blood glucose levels at t = 30 min Conclusions: These results indicate an inhibitory action against carbohydrate-digesting enzymes as the anti-diabetic mechanism of action of PPE. Nonetheless, further clinical study is required to justify its role in the treatment of diabetes.展开更多
A new genus,Wuacanthus Y.F.Deng,N.H.Xia&H.Peng(Acanthaceae),is described from the Hengduan Mountains,China.Wuacanthus is based on Wuacanthus microdontus(W.W.Sm.)Y.F.Deng,N.H.Xia&H.Peng,originally published in ...A new genus,Wuacanthus Y.F.Deng,N.H.Xia&H.Peng(Acanthaceae),is described from the Hengduan Mountains,China.Wuacanthus is based on Wuacanthus microdontus(W.W.Sm.)Y.F.Deng,N.H.Xia&H.Peng,originally published in Justicia and then moved to Mananthes.The new genus is characterized by its shrub habit,strongly 2-lipped corolla,the 2-lobed upper lip,3-lobed lower lip,2 stamens,bithecous anthers,parallel thecae with two spurs at the base,2 ovules in each locule,and the 4-seeded capsule.Phylogenetic analyses show that the new genus belongs to the Pseuderanthemum lineage in tribe Justicieae.Wuacanthus is closely related to Pseuderanthemum but differs from the latter by its shorter corolla tube and two minute spurs at the base of each anther-theca.W.microdontus is assessed with the status EN B2ab(iii)based on the IUCN Red List Categories and Criteria.展开更多
基金Supported by Mahasarakham University research grant(Grant No.5805028/2558,fiscal year 2015)
文摘Objective: To investigate the effects of the leaf ethanolic extract of Pseuderanthemum palatiferum(PPE) and its isolated phytochemicals, stigmasterol and sitosterol-3-O-b-Dglucopyranoside, against a-amylase and a-glucosidase enzyme activities both in vitro and in vivo.Methods: A concentration of maltose, which is a product released in a-amylase-catalyzing reaction, was used as an index of in vitro a-amylase activity. Meanwhile, in vitro a-glucosidase enzyme activity was indicated by the amount of liberated p-nitrophenol in a-glucosidase-catalyzing reaction. In vivo a-amylase and a-glucosidase enzyme activities were evaluated in the normal rats by using oral starch tolerance test and oral sucrose tolerance test, respectively.Results: PPE exerted a concentration-dependent inhibitory action against both aamylase and a-glucosidase in vitro with the IC_(50) values of(11.79 ± 8.10) mg/m L and(1.00 ± 0.11) mg/m L, respectively. Stigmasterol and sitosterol-3-O-b-D-glucopyranoside also exerted an in vitro a-amylase inhibition with the IC_(50) values of(59.41 ± 8.22) mg/m L and(111.19 ± 9.02) mg/m L, respectively. However, these phytochemicals did not produce a concentration-dependent inhibition against in vitro a-glucosidase activity. PPE and its isolated phytochemicals significantly decreased the blood glucose levels at t = 30 min in the oral starch tolerance test. From the sucrose tolerance test, only PPE but not its isolated phytochemicals significantly caused a depletion in the blood glucose levels at t = 30 min Conclusions: These results indicate an inhibitory action against carbohydrate-digesting enzymes as the anti-diabetic mechanism of action of PPE. Nonetheless, further clinical study is required to justify its role in the treatment of diabetes.
基金supported by the National Natural Science Foundation of China (grant nos. 30670124,31270247,31470302,31400184,31670191,31110103911)Science and Technology Basic Work (grant no. 2013FY112100)
文摘A new genus,Wuacanthus Y.F.Deng,N.H.Xia&H.Peng(Acanthaceae),is described from the Hengduan Mountains,China.Wuacanthus is based on Wuacanthus microdontus(W.W.Sm.)Y.F.Deng,N.H.Xia&H.Peng,originally published in Justicia and then moved to Mananthes.The new genus is characterized by its shrub habit,strongly 2-lipped corolla,the 2-lobed upper lip,3-lobed lower lip,2 stamens,bithecous anthers,parallel thecae with two spurs at the base,2 ovules in each locule,and the 4-seeded capsule.Phylogenetic analyses show that the new genus belongs to the Pseuderanthemum lineage in tribe Justicieae.Wuacanthus is closely related to Pseuderanthemum but differs from the latter by its shorter corolla tube and two minute spurs at the base of each anther-theca.W.microdontus is assessed with the status EN B2ab(iii)based on the IUCN Red List Categories and Criteria.
