BACKGROUND Purpureocillium lilacinum(P.lilacinum)is a saprophytic fungus widespread in soil and vegetation.As a causative agent,it is very rarely detected in humans,most commonly in the skin.CASE SUMMARY In this artic...BACKGROUND Purpureocillium lilacinum(P.lilacinum)is a saprophytic fungus widespread in soil and vegetation.As a causative agent,it is very rarely detected in humans,most commonly in the skin.CASE SUMMARY In this article,we reported the case of a 72-year-old patient with chronic lymphocytic leukemia who was admitted with cough and fever.Computed tomography revealed an infection in the right lower lobe.Bronchoalveolar lavage fluid culture and metagenomic next-generation sequencing were ultimately confirmed to have a pulmonary infection with P.lilacinum.She was eventually discharged with good outcomes after treatment with isavuconazole.CONCLUSION Pulmonary infection with P.lilacinum was exceedingly rare.While currently there are no definitive therapeutic agents,there are reports of high resistance to amphotericin B and fluconazole and good sensitivity to second-generation triazoles.The present report is the first known use of isavuconazole for pulmonary P.lilacinum infection.It provides new evidence for the characterization and treatment of clinical P.lilacinum lung infections.展开更多
Purpureocillium lilacinum(P lilacinum)is a rare pathogenic fungus,which mainly involves immunocompromised individuals.Here,we report a case of complicated multiple-organ infections involving skin,lungs,and spleen in a...Purpureocillium lilacinum(P lilacinum)is a rare pathogenic fungus,which mainly involves immunocompromised individuals.Here,we report a case of complicated multiple-organ infections involving skin,lungs,and spleen in a 63-year-old female with Evans’syndrome after 9 months of glucocorticoid treatment.Microbial examinations of skin biopsy and blood samples revealed Plilacinum infections.Posaconazole was effectivein this patient.During anti-fungitreatment,she developedvaricella-zoster virus infection and was diagnosed through next-generation sequencing examination.In conclusion,Plilacinum may affect different organ systems and is susceptible to posaconazole treatment.The molecular-based methods like microbial cell-free DNA sequencing could provide accurate and timely identification of a wide range of infections.展开更多
Green onion is one of the most consumed vegetables in Colombia. However, its production is negatively affected by the nematode Ditylenchus. To determine the species of the nematode, as well as to propose management st...Green onion is one of the most consumed vegetables in Colombia. However, its production is negatively affected by the nematode Ditylenchus. To determine the species of the nematode, as well as to propose management strategies for its control, in the present study the morphological, morphometric, and molecular characterization of the phytonematode was carried out and was used to evaluate the effect of immersion of the propagation material in water hot, application of a commercial strain of Purpureocillium lilacinum and the application of an agrochemical with insecticidal-nematicidal action to control the phytosanitary problem under field conditions. The morphological and morphometric characteristics of the nematode were similar to those reported for the type and reference populations of D. dipsaci. Based on sequences of the D2-D3 segment and Internal Transcribed Spacer-ITS of the rRNA, the presence of D. dipsaci in green onion crops in Colombia was confirmed. The application of P. lilacinum statistically showed an efficient control of D. dipsaci, at the same time that presented the highest yield, in relation to the other evaluated treatments (P ≤ 0.05).展开更多
文摘BACKGROUND Purpureocillium lilacinum(P.lilacinum)is a saprophytic fungus widespread in soil and vegetation.As a causative agent,it is very rarely detected in humans,most commonly in the skin.CASE SUMMARY In this article,we reported the case of a 72-year-old patient with chronic lymphocytic leukemia who was admitted with cough and fever.Computed tomography revealed an infection in the right lower lobe.Bronchoalveolar lavage fluid culture and metagenomic next-generation sequencing were ultimately confirmed to have a pulmonary infection with P.lilacinum.She was eventually discharged with good outcomes after treatment with isavuconazole.CONCLUSION Pulmonary infection with P.lilacinum was exceedingly rare.While currently there are no definitive therapeutic agents,there are reports of high resistance to amphotericin B and fluconazole and good sensitivity to second-generation triazoles.The present report is the first known use of isavuconazole for pulmonary P.lilacinum infection.It provides new evidence for the characterization and treatment of clinical P.lilacinum lung infections.
基金This study was supported by Grant 18JCYBJC41200 of Tianjin Municipal Science and Technology Commission Grant.
文摘Purpureocillium lilacinum(P lilacinum)is a rare pathogenic fungus,which mainly involves immunocompromised individuals.Here,we report a case of complicated multiple-organ infections involving skin,lungs,and spleen in a 63-year-old female with Evans’syndrome after 9 months of glucocorticoid treatment.Microbial examinations of skin biopsy and blood samples revealed Plilacinum infections.Posaconazole was effectivein this patient.During anti-fungitreatment,she developedvaricella-zoster virus infection and was diagnosed through next-generation sequencing examination.In conclusion,Plilacinum may affect different organ systems and is susceptible to posaconazole treatment.The molecular-based methods like microbial cell-free DNA sequencing could provide accurate and timely identification of a wide range of infections.
文摘Green onion is one of the most consumed vegetables in Colombia. However, its production is negatively affected by the nematode Ditylenchus. To determine the species of the nematode, as well as to propose management strategies for its control, in the present study the morphological, morphometric, and molecular characterization of the phytonematode was carried out and was used to evaluate the effect of immersion of the propagation material in water hot, application of a commercial strain of Purpureocillium lilacinum and the application of an agrochemical with insecticidal-nematicidal action to control the phytosanitary problem under field conditions. The morphological and morphometric characteristics of the nematode were similar to those reported for the type and reference populations of D. dipsaci. Based on sequences of the D2-D3 segment and Internal Transcribed Spacer-ITS of the rRNA, the presence of D. dipsaci in green onion crops in Colombia was confirmed. The application of P. lilacinum statistically showed an efficient control of D. dipsaci, at the same time that presented the highest yield, in relation to the other evaluated treatments (P ≤ 0.05).
