[Objectives]The paper was to investigate and identify the fungal diseases of wild and red heart kiwifruit in Qiandongnan Prefecture.[Methods]The pathogenic fungi were isolated from diseased leaves and fruits of wild a...[Objectives]The paper was to investigate and identify the fungal diseases of wild and red heart kiwifruit in Qiandongnan Prefecture.[Methods]The pathogenic fungi were isolated from diseased leaves and fruits of wild and red heart kiwifruit by tissue separation method.DNA sequencing was carried out by using the sequence analysis of ribosomal r DNA-ITS region,and molecular evolutionary trees were built by using MEGA 4.0 software.Finally,the pathogenic fungi were classified and identified by combining morphological observation.[Results]The main fungal diseases were anthracnose caused by Colletotrichum gloeosporioides on wild kiwifruit,fruit anthracnose caused by C.acutatum on red heart kiwifruit,leaf soft rot caused by Fusarium incarnatum on red heart kiwifruit,and brown spot caused by Alternaria alternata on red heart kiwifruit.[Conclusions]The study may provide some theoretical basis for the control of kiwifruit diseases in Qiandongnan Prefecture.展开更多
基金Supported by Identification and Control Analysis of Diseases and Insect Pests of Kiwifruit in Qiandongnan Prefecture(QKH H[2017]7178)Guizhou Key Laboratory of Qiandongnan Ethnic Characteristic Food Research and Development(QJH KY[2017]011)Talent Team Project of Guizhou Department of Education(QJHRCTD[2015]70)。
文摘[Objectives]The paper was to investigate and identify the fungal diseases of wild and red heart kiwifruit in Qiandongnan Prefecture.[Methods]The pathogenic fungi were isolated from diseased leaves and fruits of wild and red heart kiwifruit by tissue separation method.DNA sequencing was carried out by using the sequence analysis of ribosomal r DNA-ITS region,and molecular evolutionary trees were built by using MEGA 4.0 software.Finally,the pathogenic fungi were classified and identified by combining morphological observation.[Results]The main fungal diseases were anthracnose caused by Colletotrichum gloeosporioides on wild kiwifruit,fruit anthracnose caused by C.acutatum on red heart kiwifruit,leaf soft rot caused by Fusarium incarnatum on red heart kiwifruit,and brown spot caused by Alternaria alternata on red heart kiwifruit.[Conclusions]The study may provide some theoretical basis for the control of kiwifruit diseases in Qiandongnan Prefecture.
