AIM To observe the effects of Qiwei Baizhu Powder ( QWBZP) on rotaviral gastroenteritis in children and in animal models.``METHODS Enrolled patients were divided into two groups, and one group was treated with oral re...AIM To observe the effects of Qiwei Baizhu Powder ( QWBZP) on rotaviral gastroenteritis in children and in animal models.``METHODS Enrolled patients were divided into two groups, and one group was treated with oral rehydration solution(ORS) and the other treated with oral liquid of QWBZP. Neonate mice were orally infected with 50 μLrotavirus suspension (4 × l0s PFU/mL) and treated with ORS or oral liquid of QWBZP, respectively.``RESULTS Eighty-three cases of rotaviral gastroenteritis treated with QWBZP revealed a better efficacy than that treated with ORS (x2 - 10.8T, P<0.05). The contents of sodium and glucose as well as number of patients with positive human rotavirus antigen in stool in QWBZP group were all less than that in ORS group. In animal models,QWBZP was found effective in treating rotavirus gastroenteritis in neonate NIH mice, as compared with control groups. In QWBZP group, the mortality of infected mice was decreased by 73.3%, the body weight of infected mice was increased, the contents of sodium and glucose as well as number of mice with positive rotavirus antigen in feces were significantly reduced, and the pathological changes such as damage of small intestinal mucosa and villi were also obviously alleviated.``CONCLUSION QWBZP has effects on improving the absorptive function of small intestine, shortening the duration of diarrhea and rotavirus shedding from stool and alleviating the pathological changes of small intestine induced by rotavirus.展开更多
目的基于网络药理学的方法探讨七味白术散治疗菌群失调性腹泻(Flora imbalance diarrhea)的作用机制。方法通过中药系统药理学数据库(Traditional Chinese medicine system platform,TCMSP)获得七味白术散所含中药的主要化学成分,并收...目的基于网络药理学的方法探讨七味白术散治疗菌群失调性腹泻(Flora imbalance diarrhea)的作用机制。方法通过中药系统药理学数据库(Traditional Chinese medicine system platform,TCMSP)获得七味白术散所含中药的主要化学成分,并收集其潜在作用靶点;菌群失调性腹泻疾病靶点来源于GeneCards、DrugBank、Home-OMIM-NCBI数据库。通过UniProt数据库对药物靶基因与疾病靶基因校正,使用Draw Venn Diagrams工具获得交集基因。应用Cytoscape 3.7.1对所得的交集基因进行PPI分析、GO与KEGG通路富集分析。结果共获得七味白术散活性成分84个,药物作用靶点201个,疾病靶点177个,交集基因26个。PPI网络中,丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)、Jun原癌基因(Jun proto-oncogene)AP-1转录因子亚基(AP-1 transcription factor subunit,JUN)、雌激素受体1(Estrogen receptor 1,ESR1)、糖原合成酶激酶3β(Glycogen synthase kinase 3,GSK3B)为关键靶点,GO富集分析和KEGG通路富集分析发现,药物有效成分主要通过白细胞介素-17信号通路、AGE-RAGE信号通路以及C型凝聚素受体等信号通路参与了细胞因子产生的正调控、DNA结合转录因子活性的调节、对脂多糖的反应等生物学过程。结论本研究揭示了七味白术散治疗菌群失调性腹泻可能是多成分、多靶点、多途径的,且为进一步研究提供思路和参考。展开更多
基金Supported by the National Natural science Foundation of China,No.39270861.
文摘AIM To observe the effects of Qiwei Baizhu Powder ( QWBZP) on rotaviral gastroenteritis in children and in animal models.``METHODS Enrolled patients were divided into two groups, and one group was treated with oral rehydration solution(ORS) and the other treated with oral liquid of QWBZP. Neonate mice were orally infected with 50 μLrotavirus suspension (4 × l0s PFU/mL) and treated with ORS or oral liquid of QWBZP, respectively.``RESULTS Eighty-three cases of rotaviral gastroenteritis treated with QWBZP revealed a better efficacy than that treated with ORS (x2 - 10.8T, P<0.05). The contents of sodium and glucose as well as number of patients with positive human rotavirus antigen in stool in QWBZP group were all less than that in ORS group. In animal models,QWBZP was found effective in treating rotavirus gastroenteritis in neonate NIH mice, as compared with control groups. In QWBZP group, the mortality of infected mice was decreased by 73.3%, the body weight of infected mice was increased, the contents of sodium and glucose as well as number of mice with positive rotavirus antigen in feces were significantly reduced, and the pathological changes such as damage of small intestinal mucosa and villi were also obviously alleviated.``CONCLUSION QWBZP has effects on improving the absorptive function of small intestine, shortening the duration of diarrhea and rotavirus shedding from stool and alleviating the pathological changes of small intestine induced by rotavirus.
