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Factors Affecting the Profitability of Cement ManufacturingEnterprises in Tuyen Quang Province
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作者 Hoang Anh Dao Pham Thanh Tra Tran Thao Nguyen 《Macro Management & Public Policies》 2022年第2期20-28,共9页
This study focuses on analyzing the factors affecting the production and business efficiency of cement manufacturing enterprises in Tuyen Quang province. Production and business efficiency is measured by profitability... This study focuses on analyzing the factors affecting the production and business efficiency of cement manufacturing enterprises in Tuyen Quang province. Production and business efficiency is measured by profitability ratios including return on assets (ROA), return on equity (ROE) and return on sales (ROS). Using a quantitative research method through estimating a linear regression model based on data of two cement manufacturing companies in the period 2019-2021, the research results show that the factors affecting business efficiency of cement manufacturing enterprises in Tuyen Quang province include size of enterprise, cost ratio, average collection period, inventory turnover, fixed assets turnover and debt ratio. 展开更多
关键词 Business efficiency PROFITABILITY Cement manufacturing enterprises Tuyen quang
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Co-circulation of Aedes flavivirus,Culex flavivirus,and Quang Binh virus in Shanghai,China 被引量:3
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作者 Yuan Fang Yi Zhang +6 位作者 Zheng-Bin Zhou Wen-Qi Shi Shang Xia Yuan-Yuan Li Jia-Tong Wu Qin Liu Guang-Yi Lin 《Infectious Diseases of Poverty》 SCIE 2018年第1期740-749,共10页
Background:With increases in global travel and trade,the spread of arboviruses is undoubtedly alarming.Pathogen detection in field-caught mosquitoes can provide the earliest possible warning of transmission.Insect-spe... Background:With increases in global travel and trade,the spread of arboviruses is undoubtedly alarming.Pathogen detection in field-caught mosquitoes can provide the earliest possible warning of transmission.Insect-specific flavivirus(ISFV)has been first detected in 1991 and documented worldwide in the latest ten years.Although infection with ISFVs is apparently limited to insects,an increase in the infection rate of mosquito-borne flaviviruses may be able to induce cytopathic effects in vertebrate cells during co-infection with other human pathogens.However,little is known whether ISFVs persist in most regions of China.Methods:During the mosquito activity season in 2016,a surveillance program was carried out to detect ISFVs in mosquitoes in metropolitan Shanghai,China.The presence of ISFVs was randomly tested in different species of mosquitoes using RT-PCR-based and hemi-nested PCR assays,following by the sequencing of PCR products.Sequences from positive pooled samples were compared with those deposited in GenBank.Thereafter,sequences of representative insect flaviviruses were used for further phylogenetic and molecular evolutionary analyses.Results:Our investigations showed:(1)the presence of Aedes flavivirus(AEFV)in 11/161 pooled samples(nine pools in Songjiang District,one pool in Huangpu District,and one pool in Qingpu District)of Aedes albopictus,(2)the presence of Quang Binh virus(QBV)in 10/195 pooled samples(all in Chongming District)of Culex tritaeniorhynchus;and(3)the presence of Culex flavivirus(CxFV)in 9/228 pooled samples(six pools in Pudong New Area,two pools in Huangpu District,and one pool in Chongming District)of Cx.pipiens.Furthermore,phylogenetic analyses of the gene sequences of envelope proteins indicated that Shanghai CxFV strains belonged to the Asia/USA genotype.The overall maximum likelihood estimation values(and 95%confidence interval)for CxFV,QBV,and AEFV in mosquitoes collected in Shanghai in 2016 were 1.34(0.66-2.45),1.65(0.87-2.85),and 1.51(0.77-2.70)per 1000,respectively.Conclusions:This study reveals the presence and the geographical distribution of ISFVs,and determines the genetic variation and the infection rate of ISFVs in Shanghai,China.At least,three insect flaviviruses including ISFVs,AEFV,CxFV,and QBV,co-circulate in this area.To our knowledge,this is the first report of AEFV in China. 展开更多
关键词 Aedes flavivirus Aedes albopictus Culex flavivirus Culex pipiens Culex tritaeniorhynchus quang Binh virus
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试论中法战争中的岑毓英--岑毓英研究之二 被引量:2
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作者 施铁靖 《广西民族研究》 CSSCI 北大核心 2009年第4期89-97,共9页
法国殖民主义者1883年8月强迫越南当局签订《顺化条约》以后,便把侵略矛头指向中国。面对法国殖民主义者的侵略野心,身为云贵总督的岑毓英坚定地支持刘永福,最终成就了刘永福抗法民族英雄的威名。在国家遭受侵略的危险关头,岑毓英挺身而... 法国殖民主义者1883年8月强迫越南当局签订《顺化条约》以后,便把侵略矛头指向中国。面对法国殖民主义者的侵略野心,身为云贵总督的岑毓英坚定地支持刘永福,最终成就了刘永福抗法民族英雄的威名。在国家遭受侵略的危险关头,岑毓英挺身而出,率部出关,抗击侵略者,发明"地营"战法,克敌制胜。血战宣光,重创敌军,逐鹿山西牵制敌军主力,取得临洮大捷,和镇南关大捷一起铸成中法战争胜利的丰碑。岑毓英是中国近代史上英名不朽的爱国将领,是广西壮族人民的优秀儿子! 展开更多
关键词 中法战争 岑毓英 宣光血战 “地营”法 临洮大捷
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“越南苏武”黎光贲及其在华诗作《思乡韵录》 被引量:2
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作者 牛军凯 《东南亚研究》 CSSCI 2015年第4期99-105,共7页
明嘉靖时期,越南著名文人黎光贲,作为莫朝正使来华朝贡。因明朝尚未确认莫朝新国王,对黎光贲使团身份产生怀疑,遂将之扣留于广西南宁。18年后,该使团始得赴北京完成使命。黎光贲滞留中国长达18年,后世称其为"越南苏武"。黎光... 明嘉靖时期,越南著名文人黎光贲,作为莫朝正使来华朝贡。因明朝尚未确认莫朝新国王,对黎光贲使团身份产生怀疑,遂将之扣留于广西南宁。18年后,该使团始得赴北京完成使命。黎光贲滞留中国长达18年,后世称其为"越南苏武"。黎光贲在南宁滞留期间所著诗集《思乡韵录》,以纪念家乡先贤的方式,疏解思乡之情。该诗集为研究越南著名的进士之乡唐安慕泽提供了丰富资料。 展开更多
关键词 明朝 朝贡 莫朝 黎光贲 《思乡韵录》
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朱光潜的翻译对中国马克思主义美学的独特贡献 被引量:2
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作者 高金岭 《山东外语教学》 2015年第5期105-112,共8页
为了把握朱光潜的翻译对中国马克思主义美学的独特贡献,本文首先追溯了朱光潜翻译活动的时代背景及其与马克思主义美学翻译工作相关的人生3个阶段,并以《1844年经济学-哲学手稿》为例分析了其以变译为特色的译介方法,在此基础上作者提出... 为了把握朱光潜的翻译对中国马克思主义美学的独特贡献,本文首先追溯了朱光潜翻译活动的时代背景及其与马克思主义美学翻译工作相关的人生3个阶段,并以《1844年经济学-哲学手稿》为例分析了其以变译为特色的译介方法,在此基础上作者提出,朱光潜经过第一次美学大辩论,受《1844年经济学-哲学手稿》的启发初步建立了马克思主义美学实践观后,在20世纪70年代后期中国意识形态重建的背景下,凭借自己马克思主义美学家与翻译家的双重身份,通过对《1844年经济学-哲学手稿》的重新翻译与阐发,树立了马克思主义实践美学的新形象,继而借助对一系列西方经典美学著作的翻译及其包含的实践观点的进一步梳理,恢复了马克思主义人道主义的本来面目,破除了教条主义对马克思主义美学发展造成的阻碍,推动了中国马克思主义美学的健康发展。 展开更多
关键词 朱光潜 翻译 中国马克思主义美学 《1844年经济学-哲学手稿》 独特贡献
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Agent Blue Spraying in the Mekong Delta during the Vietnam War: Fate of the Arsenic Based Herbicide Weapon Used to Destroy Rice Crop and Mangrove Forests 被引量:1
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作者 Kenneth R. Olson Larry Cihacek 《Open Journal of Soil Science》 CAS 2022年第7期253-294,共42页
Agent Blue, a mixture of cacodylic acid (CH<sub>3</sub>) As O<sub>2</sub>H) and sodium cacodylate (C<sub>2</sub>H<sub>6</sub> AsNaO<sub>2</sub>), was a tacti... Agent Blue, a mixture of cacodylic acid (CH<sub>3</sub>) As O<sub>2</sub>H) and sodium cacodylate (C<sub>2</sub>H<sub>6</sub> AsNaO<sub>2</sub>), was a tactical arsenic-based herbicide used during the Vietnam War to destroy grasses and rice crops. Natural and synthetic sources of arsenic can degrade into water-soluble forms and persist in groundwater and potentially contribute to elevating As levels in drinking water. The United States Department of Defense (DOD) and United States Department of Agricultural (USDA) Operation Ranch Hand records for tactical herbicides including Agent Blue sprayed in southern Vietnam during the Vietnam War (1961-1971) are very detailed, rather complete and publicly available. The same is not true for tactical herbicides sprayed by the Republic of Vietnam (RV) during the Khai Quang program which was supported by the U.S. Army, U.S. Navy and Central Intelligence Agency (CIA) in the Mekong Delta. Agent Blue was sprayed by the RV military for three years before the official start of the American-Vietnam War. Few, if any, RV military, US Army, US Navy and CIA spray records exist from 1962 to 1965. Vietnam War veterans, historians and scholars have reported the spraying of 3.2 million liters (468,008 kg As) of Agent Blue on rice paddies and mangrove forests in the Mekong Delta and Central Highlands by the RV military with the support of the US Army, US Navy and CIA. The Institute of Medicine estimated that 3.2 million liters (468,000 kg As) were sprayed during the RV Khai Quang program. This was in addition to the U.S. Air Force’s Operation Ranch Hand spraying of the tactical herbicide Agent Blue primarily by C-123 aircraft. The Operation Ranch Hand missions maintained location and quantities of herbicides sprayed (over 4,712,000 liters (664,392 kg As) from 1961-1971. The RV military and US military (Army and Navy) spray equipment included hand and backpack sprayers, sprayers mounted on Brown Water Navy boats, on Army track vehicles and Army land-based helicopters and helicopters based on the decks of Blue Water Navy ships. Some of these spray missions were a military secret and spray records were classified or if kept were not maintained. Agent Blue containing cacodylic acid had a short half-life and degraded to water-soluble arsenic, which was released into the surface water and/or leached into the groundwater. Once the water-soluble arsenic leached into the Vietnam Mekong Delta groundwater, the arsenic-rich water was pumped back to the surface by tens of thousands of tube wells for urban and agricultural use. The primary objectives of this research are to explore the conditions during the Vietnam War under which 1) the RV military herbicide spray program with the support of the US Navy, CIA and US Army, and 2) the US Air Force spray program during Operation Ranch Hand may have significantly contributed to the natural and anthropic As spikes found in the Mekong Delta today. The environmental impacts of Agent Blue, on the Menominee River at manufacturing sites in the United States, were studied to identify possible As remediation and mitigation strategies. The lessons previously learned at the manufacturing sites in Wisconsin and Michigan, United States can be considered and applied to the Mekong Delta to help mitigate and remediate the arsenic-rich surface water, soil, sediment and groundwater found in the Mekong Delta. 展开更多
关键词 Operation Ranch Hand Khai quang program Agent Blue ARSENIC Cacodylic Acid North Vietnam Army Republic of Vietnam Democratic Republic of Vietnam American-Vietnam War
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南宋名臣胡铨交游考——以《戊午上高宗封事》为中心 被引量:1
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作者 彭洁明 《湖南工程学院学报(社会科学版)》 2011年第4期70-74,共5页
胡铨是"南宋四名臣"之一,肝胆忠义,颇有令名,而半生岭海,仕途多蹇。宋高宗绍兴八年,胡铨以《上高宗封事》进谏,触忤权奸,开始了长达二十余年的贬谪、拘禁、编管的生涯,此文可谓胡铨一生的关键点,以此为中心考察胡铨的交游,以... 胡铨是"南宋四名臣"之一,肝胆忠义,颇有令名,而半生岭海,仕途多蹇。宋高宗绍兴八年,胡铨以《上高宗封事》进谏,触忤权奸,开始了长达二十余年的贬谪、拘禁、编管的生涯,此文可谓胡铨一生的关键点,以此为中心考察胡铨的交游,以见出作为"名臣"的胡铨其人其行对南宋初士风的影响。 展开更多
关键词 胡铨 《戊午上高宗封事》 交游
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两司马治史之比较研究
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作者 俞文冉 陈乃宣 《武汉大学学报(人文科学版)》 CSSCI 2001年第6期707-713,共7页
司马迁和司马光是我国历史上的两位伟大的史学家 ,他们撰写的《史记》和《资治通鉴》对我国史学产生了深远的影响。他们二人处于不同的历史时代 ,其著作也有明显的不同。从体例上来讲 ,《史记》为纪传体 ,《资治通鉴》为编年体。另外他... 司马迁和司马光是我国历史上的两位伟大的史学家 ,他们撰写的《史记》和《资治通鉴》对我国史学产生了深远的影响。他们二人处于不同的历史时代 ,其著作也有明显的不同。从体例上来讲 ,《史记》为纪传体 ,《资治通鉴》为编年体。另外他们二人在历史视野、对待史实的情感、思想倾向以及材料的取舍等方向也有较大的区别。 展开更多
关键词 司马迁 《史记》 司马光 《资治通鉴》 中国 史学 思想倾向
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Simulation of Flood in the Thach Han-Ben Hai River Basin(Vietnam)under the Influence of the Upstream Reservoir System
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作者 Ngo Chi Tuan Can Thu Van +1 位作者 Nguyen Huu Tuan Nguyen Hai Au 《Journal of Environmental Science and Engineering(B)》 2021年第6期227-236,共10页
The central provinces in Vietnam always suffer from the negative impacts of floods every year,especially in the downstream areas.Quang Tri province in the TBRB(Thach Han-Ben Hai River Basin)is one of the provinces suf... The central provinces in Vietnam always suffer from the negative impacts of floods every year,especially in the downstream areas.Quang Tri province in the TBRB(Thach Han-Ben Hai River Basin)is one of the provinces suffering heavy damage caused by floods.A 1-dimensional hydrodynamic model was researched and applied connecting 2 dimensions in the MIKE model set(MIKE FLOOD)to simulate inundation level,inundation time,flood flow velocity for communes in TBRB.Simulation results for 111 communes in Quang Tri province show that:39 communes(35%)are not flooded;3 communes are flooded below 0.5 m;15 communes are flooded from 0.5-1.0 m,flooding time is about 1 day;30 communes are flooded from 1.0-2.0 m,inundation time is about 2 days;30 communes are flooded over 2.0 m,flooded for about 3 days,especially 3 communes are flooded over 4.0 m.This result helps to develop flood prevention plans for localities in the province. 展开更多
关键词 Thach Han-Ben Hei River basin flood simulation quang Tri province hydrodynamic model
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以咳嗽为主要症状的哮喘治疗观察
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作者 林定曾 萧正伦 《广州医学院学报》 1991年第2期74-78,共5页
14例“咳嗽型哮喘”的患者给予服用中药咳喘定煮散为实验组,另14例则服用支气管解痉药作对照治疗期共两个月。于治疗前及治疗后2-12个月进行吸入组织胺支气管激发试验。结果表明实验组有13/14例症状消失,一例转化为哮喘,支激试验转阴者... 14例“咳嗽型哮喘”的患者给予服用中药咳喘定煮散为实验组,另14例则服用支气管解痉药作对照治疗期共两个月。于治疗前及治疗后2-12个月进行吸入组织胺支气管激发试验。结果表明实验组有13/14例症状消失,一例转化为哮喘,支激试验转阴者达10/14例。而对照组则分别为9/14、4/14和3/14例。统计学结果表明实验组治疗有效率(92.9%)和支气管激发试验转阴率(71.4%)都显著高于对照组(50%和21.4%),P值为<0.05和<0.01,从而提示中药咳喘定煮散治疗本症比支气管解痉剂有更好疗效,其机制在于能改善气道高反应性。 展开更多
关键词 气道高反应性 咳喘定煮散
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越南广宁晋磨锑矿床变质岩石特征及原岩恢复
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作者 季根源 戴塔根 +1 位作者 孔令湖 姜爱玲 《四川地质学报》 2015年第1期43-46,共4页
晋磨锑矿床位于印支地块和华南地块碰撞界限的边缘,紧靠红河走滑大断裂的东侧,属于华南地块。对研究区变质岩的岩石学、地球化学研究表明,该矿床变质岩均为浅变质的副变质岩,其原岩主要为杂砂岩,其次为泥质砂岩、含炭泥质砂岩,粘土岩。
关键词 锑矿 变质岩 原岩 越南晋磨
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褚童子故事与越南佛教起源
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作者 阮春宏 《长江师范学院学报》 2012年第3期70-73,139,共4页
关于越南佛教的起源问题,不是现代学者才提出来的。早在一千多年前,符圣灵仁猗兰皇太后于1091年曾将其事提问过通辨国师,通辨引昙迁法师传来禀奏,其事在《禅苑集英语录》中记录非常清楚①。与《禅苑集英语录》同时代的《岭南摭怪》记载... 关于越南佛教的起源问题,不是现代学者才提出来的。早在一千多年前,符圣灵仁猗兰皇太后于1091年曾将其事提问过通辨国师,通辨引昙迁法师传来禀奏,其事在《禅苑集英语录》中记录非常清楚①。与《禅苑集英语录》同时代的《岭南摭怪》记载了《一夜泽传》,详述公元前三世纪褚童子向佛光法师学佛道的情况。本文通过初步收集、爬梳相关材料,对其进行分析、讨论,提出了越南佛教起源的新看法,也为越南佛教历史研究提供一些有益的启示。 展开更多
关键词 褚童子 佛光法师 越南佛教 起源
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越南广宁省旅游产业发展分析
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作者 LANH Thihoa 《辽宁工程技术大学学报(社会科学版)》 2016年第2期177-179,共3页
针对越南广宁省旅游业发展问题,采用SWOT分析方法,分析了广宁省旅游业现状,广宁省旅游资源丰富,知名度高,但旅游系统不完善,面对全国重点发展旅游业的机遇,建设发挥优势,直面问题,促进广宁省旅游业的健康发展。
关键词 广宁省 旅游业 旅游管理 文化品牌 区域优势
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彭湃农民运动理论在广宁的实践与发展
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作者 栾盈菊 《韶关学院学报》 2011年第11期97-100,共4页
1924年,彭湃曾两次去广宁指导广宁农民运动,并且都取得重大成效。这得益于彭湃既有的成熟革命理论,这些理论在广宁革命运动中得到了充分发挥。回广州以后,彭湃又将这些理论做了进一步发展。所以,广宁农民运动是彭湃革命生涯中的重要阶段。
关键词 彭湃 广宁 农民运动 减租运动 武装斗争
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Long-distance spread of Tembusu virus,and its dispersal in local mosquitoes and domestic poultry in Chongming Island,China
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作者 Yuan Fang Tian Hang +7 位作者 Li-Min Yang Jing-Bo Xue Ryosuke Fujita Xue-Song Feng Tian-Ge Jiang Yi Zhang Shi-Zhu Li Xiao-Nong Zhou 《Infectious Diseases of Poverty》 SCIE CAS CSCD 2023年第3期94-95,共2页
Background Chongming Island in China serves as a breeding and shelter point on the East Asian–Australasian Flyway.The resting frequency of migratory birds,abundance of mosquito populations,and the popular domestic po... Background Chongming Island in China serves as a breeding and shelter point on the East Asian–Australasian Flyway.The resting frequency of migratory birds,abundance of mosquito populations,and the popular domestic poultry industry pose a potential risk of mosquito-borne zoonotic diseases.The aim of this study is to explore the role of migratory birds in the spread of mosquito-borne pathogens and their prevalent status on the island.Methods We conducted a mosquito-borne pathogen surveillance in 2021,in Chongming,Shanghai,China.Approxi‑mately 67,800 adult mosquitoes belonging to ten species were collected to investigate the presence of faviviruses,alphaviruses,and orthobunyaviruses by RT-PCR.Genetic and phylogenetic analyses were conducted to explore the virus genotype and potential nature source.Serological survey was performed by ELISA to characterize Tembusu virus(TMUV)infection among domestic poultry.Results Two strains of TMUV and Chaoyang virus(CHAOV)and 47 strains of Quang Binh virus(QBV)were detected in 412 mosquito pools,with the infection rate of 0.16,0.16,and 3.92 per 1000 Culex tritaeniorhynchus,respectively.Fur‑thermore,TMUVs viral RNA was found in serum samples of domestic chickens and faecal samples of migratory birds.Antibodies against TMUV were detected in domestic avian serum samples,generally ranging from 44.07%in pigeons to 55.71%in ducks.Phylogenetic analyses indicated that the TMUV detected in Chongming belonged to Cluster 3,Southeast Asia origin,and most closely related to the CTLN strain,which caused a TMUV outbreak in chickens in Guangdong Province in 2020,but distant from strains obtained previously in Shanghai,which were involved in the 2010 TMUV outbreak in China.Conclusions We speculate that the TMUV was imported to Chongming Island through long-distance spreading by migratory birds from Southeast Asia,followed by spill over and transmission in mosquitoes and domestic avian species,threatening the local domestic poultry.In addition,the expansion and prevalence of insect-specifc favivi‑ruses and its simultaneous circulation with mosquito-borne virus are worthy of close attention and further study. 展开更多
关键词 Chaoyang virus Emerging zoonotic vector-borne disease Insect-specifc favivirus Mosquito-borne virus quang Binh virus
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The effectiveness of light emitting diode(LED)lamps in the offshore purse seine fishery in Vietnam
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作者 Nguyen Dang Nhat Do Thanh Tien +4 位作者 Truong Van Dan Nguyen Duy Quynh Tram Nguyen Quang Lich Ho Dang Phuc Nguyen Ngoc Phuoc 《Aquaculture and Fisheries》 CSCD 2023年第5期551-557,共7页
Fishing with artificial light has become one of the most advanced,efficient,and common methods for the night-time purse seining in Vietnam.This study evaluated the radiation spectrum,CIE chromaticity coordinates,corre... Fishing with artificial light has become one of the most advanced,efficient,and common methods for the night-time purse seining in Vietnam.This study evaluated the radiation spectrum,CIE chromaticity coordinates,correlated color temperature(CCT),catch rate,fuel consumption,and CO_(2)emissions when using Light emitting diode(LED)lamps(0.196 kW)in comparison with the use of metal halide(MH)lights(1 kW)in the offshore purse seine fishery in Quang Tri province,Vietnam.The fishing efficiency of the purse seine fishing boats using LED lamps has increased 1.58 times in catch rate than MH lights,although the energy consumption of LED lamp is 4 times smaller.Fuel consumption of boats per trip using LED lamps was one third of that using MH lights.The use of LED reduced the radiation spectrum,especially the intense UV radiation which negatively affects the health of fishermen.This study also showed the potential of CO_(2)emission reduction up to 1.09 tons of CO_(2)per trip per boat from the use of LED lamps in the offshore purse seine fishing boats. 展开更多
关键词 Light emitting diode(LED)lamp Metal halide(MH)light Offshore purse seine fishery quang tri province VIETNAM
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首次从我国蚊虫中分离到类似广平病毒 被引量:5
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作者 冯云 李鸿斌 +5 位作者 朱进 章域震 杨卫红 范建华 梁国栋 张海林 《病毒学报》 CAS CSCD 北大核心 2014年第1期57-61,共5页
为掌握云南省西双版纳州勐海县蚊媒病毒分布状况,用诱蚊灯采集蚊虫标本,用细胞培养法分离病毒,对阳性分离物进行分子生物学鉴定。2012年7月,在勐海县打洛镇采集蚊虫32批共1 468只,其中三带喙库蚊(Culex tritaeniorhynchus)28批1 383只,... 为掌握云南省西双版纳州勐海县蚊媒病毒分布状况,用诱蚊灯采集蚊虫标本,用细胞培养法分离病毒,对阳性分离物进行分子生物学鉴定。2012年7月,在勐海县打洛镇采集蚊虫32批共1 468只,其中三带喙库蚊(Culex tritaeniorhynchus)28批1 383只,致倦库蚊(Culex quinquefascitatus)2批66只,按蚊(Anopheles)2批19只。用金黄地鼠肾细胞(Golden hamster kidney cell,简称BHK-21)和白纹伊蚊细胞(Aedes albopictus cell,简称C6/36)进行病毒分离,结果有2批三带喙库蚊标本(BNDL1205和BNDL1227)能引起C6/36细胞产生聚合病变,而不能引起BHK-21细胞病变。用黄病毒属特异性引物对这2株阳性分离物进行RT-PCR,扩增得到800bp左右的条带并进行核苷酸序列测定。对核苷酸序列的系统进化分析表明,这2株病毒与分离自越南的广平病毒(Quang Binh virus,QBV)VN180株亲缘性最近,处在同一进化分支;与VN180株的核苷酸同源性分别为83.4%和82.9%,而与既往国内外分离的18株蚊虫黄病毒的核苷酸差异较大。结果表明BNDL1205和BNDL1227病毒为类似QBV,此为国内首次报道。 展开更多
关键词 库蚊黄病毒 广平病毒 系统进化树 同源性分析
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广东省三株蚊媒黄病毒的鉴定和系统进化分析 被引量:2
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作者 吴德 李柏生 +5 位作者 谈琦琪 张欣 张欢 曹玉玺 王环宇 梁国栋 《病毒学报》 CAS CSCD 北大核心 2019年第2期240-246,共7页
为了解广东省蚊媒病毒的种类和分子特征,本研究对从蚊子中分离的三株黄病毒提取病毒核酸并进行二代测序,经序列拼接和比对,基因组序列中缺失的gap通过RT-PCR进行扩增填补。用MEGA软件登录GenBank下载黄病毒代表株序列并绘制进化树,同时... 为了解广东省蚊媒病毒的种类和分子特征,本研究对从蚊子中分离的三株黄病毒提取病毒核酸并进行二代测序,经序列拼接和比对,基因组序列中缺失的gap通过RT-PCR进行扩增填补。用MEGA软件登录GenBank下载黄病毒代表株序列并绘制进化树,同时用ClustalW2软件与新毒株进行氨基酸同源性比对和编码框序列的分析。结果显示利用二代测序技术获得了三株病毒基因组序列的96.35%~98.26%,并用RT-PCR成功补齐所有gap,序列比对显示其中一株与黄斑库蚊病毒核苷酸和氨基酸同源性98.51%,两株病毒与广平病毒的核苷酸同源性分别为97.30%和89.78%。三株病毒都属于黄病毒家族中的未分类黄病毒或昆虫特异性黄病毒,编码区序列中C蛋白的同源性最低,NS5的同源性最高。本研究发现的三株蚊媒黄病毒中,库蚊黄斑病毒在国内为首次鉴定;两株广平病毒核苷酸同源性存在较大差异,可能存在不同基因亚型。 展开更多
关键词 致倦库蚊 黄斑库蚊病毒(CTFV) 广平病毒(QBV) 系统进化分析
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新规则试行后竞技武术太极拳自选套路技术发展现状研究 被引量:9
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作者 刘凤霞 《北京体育大学学报》 CSSCI 北大核心 2006年第3期418-420,共3页
本文根据武术竞赛新规则修改实施后,对竞技武术自选套路太极拳技术发展状况及其相应的训练对策进行比较分析研究,讨论与分析竞技自选套路太极拳技术发展现状,找出与其相适应的训练对策,有助于教练员和运动员在训练中找到相关合理、科学... 本文根据武术竞赛新规则修改实施后,对竞技武术自选套路太极拳技术发展状况及其相应的训练对策进行比较分析研究,讨论与分析竞技自选套路太极拳技术发展现状,找出与其相适应的训练对策,有助于教练员和运动员在训练中找到相关合理、科学的对策。 展开更多
关键词 太极拳 规则 竞技武术 自选套路 技术发展现状 训练对策
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我国西北地区蚊虫标本中广平病毒的检测 被引量:1
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作者 李樊 殷启凯 +10 位作者 胡伟军 周莉薇 付士红 张少白 韩坤 聂凯 何英 许晓光 许松涛 梁国栋 王环宇 《疾病监测》 CAS CSCD 北大核心 2022年第3期373-376,共4页
目的对我国西北地区蚊虫携带病毒开展调查,并快速发现重要的蚊媒病毒。方法通过形态学方法对采集蚊虫进行分类鉴定,深度测序和宏基因组分析方法分析蚊虫携带的病毒,结合Sanger测序法补全病毒基因组全序,应用系统进化分析方法分析病毒分... 目的对我国西北地区蚊虫携带病毒开展调查,并快速发现重要的蚊媒病毒。方法通过形态学方法对采集蚊虫进行分类鉴定,深度测序和宏基因组分析方法分析蚊虫携带的病毒,结合Sanger测序法补全病毒基因组全序,应用系统进化分析方法分析病毒分子遗传特征。结果2019年7月采集自陕西省的中华按蚊标本和宁夏回族自治区的三带喙库蚊标本均检测到黄病毒属广平病毒。进化分析结果显示,这两株广平病毒序列与在中国辽宁省发现的广平病毒JM17156株具有较近亲缘关系,核酸序列同源性为99.8%。结论本研究首次在我国西北地区中华按蚊、三带喙库蚊标本中检测到广平病毒。 展开更多
关键词 广平病毒 黄病毒 虫媒病毒 深度测序 宏基因组
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