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Characterization and expression analysis of a novel RING-HC gene,ZmRHCP1,involved in brace root development and abiotic stress responses in maize 被引量:1
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作者 LI Wen-lan SUN Qi +3 位作者 LI Wen-cai YU Yan-li ZHAO Meng MENG Zhao-dong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第9期1892-1899,共8页
RING is a really interesting new gene which plays important regulatory roles in many developmental processes as well as in plant-environment interactions. In the present report, the Zm RHCP1 gene encoding a putative R... RING is a really interesting new gene which plays important regulatory roles in many developmental processes as well as in plant-environment interactions. In the present report, the Zm RHCP1 gene encoding a putative RING-HC protein was isolated from maize and characterized. The Zm RHCP1 protein contained 310 amino acid residues with a conserved RINGHC zinc-finger motif and two transmembrane(TM) domains. Zm RHCP1 was expressed ubiquitously in various organs(root, stem, leaf, seedling, immature ear, and tassel), but its transcript levels were higher in vegetative organs than in reproductive organs. Moreover, the expression pattern of Zm RHCP1 in brace roots indicated that Zm RHCP1 functions in brace root initiation. In addition, Zm RHCP1 expression was regulated by abiotic stresses. The expression results suggested that Zm RHCP1 plays important roles in brace root development and abiotic stress responses. The findings of the present study provide important information to help us understand the function of Zm RHCP1 in maize. 展开更多
关键词 ring-hc zinc-finger brace root expression abiotic stresses maize
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Mg-Gd-Y-Zr合金的热裂性能 被引量:15
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作者 黄玉光 吴国华 +3 位作者 侯正全 陈斌 迟秀梅 丁文江 《特种铸造及有色合金》 CAS CSCD 北大核心 2009年第2期181-183,共3页
分别采用约束热裂棒法、热裂环法以及热裂综合测试系统来评定Mg-10Gd-3Y-Zr合金的热裂倾向性,并与常用AZ91D镁合金的热裂性能进行了比较。研究发现,Mg-10Gd-3Y-Zr合金和AZ91D合金的热裂倾向性为:约束热裂棒法评定的热裂倾向系数分别为8... 分别采用约束热裂棒法、热裂环法以及热裂综合测试系统来评定Mg-10Gd-3Y-Zr合金的热裂倾向性,并与常用AZ91D镁合金的热裂性能进行了比较。研究发现,Mg-10Gd-3Y-Zr合金和AZ91D合金的热裂倾向性为:约束热裂棒法评定的热裂倾向系数分别为8和32,热裂综合测试系统测定其抗热裂力分别约为340N和40N,热裂环法测定Mg-10Gd-3Y-Zr合金和AZ91D合金的临界断裂直径分别为53mm和48mm。试验结果表明,Mg-10Gd-3Y-Zr合金的热裂倾向性低于AZ91D合金的热裂倾向性。 展开更多
关键词 Mg-10Gd-3Y—Zr合金 热裂倾向性 约束热裂棒法 热裂环法
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利用GATEWAY^(TM)技术快速构建番木瓜环斑病毒HC-pro基因的RNAi表达载体 被引量:1
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作者 高艳梅 翟金玲 黄惜 《热带作物学报》 CSCD 2009年第4期505-509,共5页
通过RT-PCR扩增番木瓜环斑PRSV病毒HC-pro基因,序列比对结果表明,克隆到的该基因片段与已知的海南或华南地区已克隆的HC-pro基因只有约92%的同源性,说明PRSV病毒具有较大的变异性。为了培育番木瓜转基因抗病植株,利用pWATERGATE载体和GA... 通过RT-PCR扩增番木瓜环斑PRSV病毒HC-pro基因,序列比对结果表明,克隆到的该基因片段与已知的海南或华南地区已克隆的HC-pro基因只有约92%的同源性,说明PRSV病毒具有较大的变异性。为了培育番木瓜转基因抗病植株,利用pWATERGATE载体和GATEWAYTM技术,成功构建了番木瓜环斑病毒HC-pro基因的RNAi表达载体。具体步骤如下:①设计带特异性重组序列attB的HC-pro基因PCR的引物;②利用该引物进行RT-PCR扩增HC-pro基因;③通过BP反应,将扩增得到的HC-pro基因序列插入入门载体pDONR201中;④通过LR反应将HC-pro基因序列从pDONR201重组到目标载体pWATERGATE中,得到PRSVHC-pro基因的RNAi表达载体。 展开更多
关键词 番木瓜环斑病毒 GATEWAYTM技术 RNAI HC-PRO基因
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