RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer cells.However,it remains unclear whether RNF187 exhibits comparable functionality in the development of germli...RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer cells.However,it remains unclear whether RNF187 exhibits comparable functionality in the development of germline cells.To investigate thepotential involvement of RNF187 in germ cell development,we conducted interference and overexpression assays using GC-2 cells,a mouse spermatocyte-derived cell line.Our findings reveal that the interaction between RNF187 and histone H3 increases theviability,proliferation,and migratory capacity of GC-2 cells.Moreover,we provide evidence demonstrating that RNF187 interactswith H3 and mediates the ubiquitination of H3 at lysine 57(K57)or lysine 80(K80),directly or indirectly resulting in increasedcellular transcription.This is a study to report the role of RNF187 in maintaining the development of GC-2 cells by mediatinghistone H3 ubiquitination,thus highlighting the involvement of the K57 and K80 residues of H3 in the epistatic regulation of genetranscription.These discoveries provide a new theoretical foundation for further comprehensive investigations into the functionof RNF187 in the reproductive system.展开更多
Objective:To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and its relationship with prognosis.Methods:The mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of ...Objective:To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and its relationship with prognosis.Methods:The mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of HCC patients were determined by qRT-PCR and Western blot analysis,and RNF187 expressions in HCC were detected by immunohistochemistry.The relationship of RNF187 expression with clinicopathologic factors and prognosis in HCC patients were analyzed.Results:RNF187 expressed highly in HCC tissues compared with the corresponding adjacent liver tissues both in mRNA and protein level,and significantly higher in HCC tissue from patients with intrahepatic metastases or vascular invasion than those in HCC tissue from patients without intrahepatic metastases and vascular invasion(all P<0.05).The 5-year survival rate of the high expression group was lower than that of the low expression group(P<0.05).Cox regression analysis revealed that high RNF187 expression was independent risk factor for postoperative survival(HR=1.313,95%CI=1.128~1.537,P<0.001).Conclusion:High expression of RNF187 in HCC is closely related to tumor prognosis,which may become a new index to evaluate the clinical diagnosis and prognosis of liver cancer.展开更多
基金supported by the National Natural Science Foundation ofChina(82271633 to BZ and 82201762 to TTG)the Gusu Health Talent Programof Suzhou(GSWS2020068 to BZ)+2 种基金the Top Talent Support Program for Youngand Middle-aged People of Wuxi Health Committee(BJ2020047 to YBW)theScience and Technology Project of Changzhou(CJ20220143 to TTG)the Changzhou Health Committee Funded Young Investigator Training Project(CZQM2020099 to TTG)。
文摘RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer cells.However,it remains unclear whether RNF187 exhibits comparable functionality in the development of germline cells.To investigate thepotential involvement of RNF187 in germ cell development,we conducted interference and overexpression assays using GC-2 cells,a mouse spermatocyte-derived cell line.Our findings reveal that the interaction between RNF187 and histone H3 increases theviability,proliferation,and migratory capacity of GC-2 cells.Moreover,we provide evidence demonstrating that RNF187 interactswith H3 and mediates the ubiquitination of H3 at lysine 57(K57)or lysine 80(K80),directly or indirectly resulting in increasedcellular transcription.This is a study to report the role of RNF187 in maintaining the development of GC-2 cells by mediatinghistone H3 ubiquitination,thus highlighting the involvement of the K57 and K80 residues of H3 in the epistatic regulation of genetranscription.These discoveries provide a new theoretical foundation for further comprehensive investigations into the functionof RNF187 in the reproductive system.
基金Hainan key research and development plan(No.ZDYF2019120)National Natural Science Foundation of China(No.81660489,81260367,81160310)Natural Science Foundation of Hainan Province(No.819QN356)
文摘Objective:To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and its relationship with prognosis.Methods:The mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of HCC patients were determined by qRT-PCR and Western blot analysis,and RNF187 expressions in HCC were detected by immunohistochemistry.The relationship of RNF187 expression with clinicopathologic factors and prognosis in HCC patients were analyzed.Results:RNF187 expressed highly in HCC tissues compared with the corresponding adjacent liver tissues both in mRNA and protein level,and significantly higher in HCC tissue from patients with intrahepatic metastases or vascular invasion than those in HCC tissue from patients without intrahepatic metastases and vascular invasion(all P<0.05).The 5-year survival rate of the high expression group was lower than that of the low expression group(P<0.05).Cox regression analysis revealed that high RNF187 expression was independent risk factor for postoperative survival(HR=1.313,95%CI=1.128~1.537,P<0.001).Conclusion:High expression of RNF187 in HCC is closely related to tumor prognosis,which may become a new index to evaluate the clinical diagnosis and prognosis of liver cancer.
