Phytochemical investigations of the aerial parts of Reineckia carnea, collected in Yunnan Province of China, were conducted to explore new chemical constituents. A series of chromatographic and spectroscopic procedure...Phytochemical investigations of the aerial parts of Reineckia carnea, collected in Yunnan Province of China, were conducted to explore new chemical constituents. A series of chromatographic and spectroscopic procedures were utilized on the aqueous solution after partitioned with ethyl acetate, which resulted in the separation of a new furostanol-type glycoside and four known compounds. The structures of the isolated compounds were elucidated on the basis of spectroscopic techniques（1D and 2D NMR, IR, HRESIMS） as 26-O-β-D-glucopyranosyl-（25S）-5β-furost-20（22）-en-1α,3β,26-triol-1-O-α-L-arabinopyranosyl-（1→2）-[α-Lrhamnopyranos-yl]-3-O-α-L-rhamnopyranoside（1）,（1β,3β,16β,22S）-cholest-5-en-1,3,16,22-tetrol-1,16-di-（β-D-glucopyranoside）（2）, diosgenin（3）, β-sitosterol（4）, ecdysterone（5）.展开更多
The aim of current study was to investigate the chemical components of the aerial part of Reineckia carnea,collected in Yunnan Province of China.Repeated column chromatography（CC）separations were performed to isolat...The aim of current study was to investigate the chemical components of the aerial part of Reineckia carnea,collected in Yunnan Province of China.Repeated column chromatography（CC）separations were performed to isolate and purify components. Compounds were identified by the analysis of their 1D and 2D NMR data as well as IR and MS spectra.A new pregnane-type glycoside,named 1α,3β-diol-5β-pregn-16-ene-20-one-1-O-α-L-arabinosyl-（1→2）-α-L-rhamnoside（1）,together withβ-amyrin（2）, stigmasterol（3）,α-spinasterol-3-O-β-D-glucoside（4）,naringenin（5）,β-sitosterol（6）and daucosterol（7）,were isolated from ethyl acetate（EtOAc）and normal butanol（n-BuOH）extracts.Compounds 2,4,5 were isolated from this plant for the first time.展开更多
Two new isospirostanol sapogenins named （25R）-5/%spirostane-（1αα 3a）-diol （1） and （25R）-5/%spirostane-（1α, 2α, 3α, 4α）- tetrol （2） were isolated from the whole herb ofReineckia carnea （Andr.） Kunth...Two new isospirostanol sapogenins named （25R）-5/%spirostane-（1αα 3a）-diol （1） and （25R）-5/%spirostane-（1α, 2α, 3α, 4α）- tetrol （2） were isolated from the whole herb ofReineckia carnea （Andr.） Kunth. The structures of the sapogenins were elucidated by chemical and spectral methods. methods展开更多
以苗族常用药材吉祥草(Reineckia carnea(Andr.)Kunth)为试材,采用盆栽控制试验,设置了对照(CK)、单独增二氧化碳(eC:CO_(2)浓度为(800±20)μmol·mol^(-1))、单独施氮(eN:施加5 g NH_(4)NO_(3))、同时增二氧化碳与施氮(eC+eN:...以苗族常用药材吉祥草(Reineckia carnea(Andr.)Kunth)为试材,采用盆栽控制试验,设置了对照(CK)、单独增二氧化碳(eC:CO_(2)浓度为(800±20)μmol·mol^(-1))、单独施氮(eN:施加5 g NH_(4)NO_(3))、同时增二氧化碳与施氮(eC+eN:CO_(2)浓度为(800±20)μmol·mol^(-1),且施加5 g NH_(4)NO_(3))4个处理,研究CO_(2)浓度升高、氮沉降及二者的交互作用对吉祥草的生长及其药用成分的影响,以期为吉祥草在药用生产上提供参考依据。结果表明:1)升高CO_(2)浓度和施氮或同时升高CO_(2)浓度和施氮均显著促进了吉祥草的形态生长,总生物量和可溶性蛋白质含量显著增加,茎中的碳含量显著降低,超氧化物歧化酶(SOD)活性无显著变化;2)施氮处理下全氮含量显著增加,过氧化氢酶(CAT)活性、根和叶中的皂苷含量显著下降,在此基础上升高CO_(2)浓度可缓解皂苷下降,CAT活性显著升高,内源激素含量在施氮或同时升高CO_(2)浓度和施氮处理下显著增加。综上所述,在实际生产过程中,可适当增加CO_(2)浓度,氮肥浓度不宜过高,有利于吉祥草的生长,并保证其药用品质不受损害。展开更多
[Objective] This study was conducted to develop potential natural plant products for controlling walnut blight pathogen and other bacteria. [Method] Inhibitory effects of extracts obtained from 15 plants with 3 solven...[Objective] This study was conducted to develop potential natural plant products for controlling walnut blight pathogen and other bacteria. [Method] Inhibitory effects of extracts obtained from 15 plants with 3 solvents on bacteria were investi- gated by disk diffusion method. [Results] Except the extracts from Magnolia grandi- flora and Typha orientalis, extracts of 13 plant leaves presented inhibitory effects on 5 bacteria strains to certain degrees. Among them, the effect of water extract of Aesculu schinensis on Bacillus sp. XHE8 was the strongest, with inhibition zone di- ameter reaching （31.3＋3.9） mm and the ratio to control above 5.0. Four of the 5 tested strains were sensitive to the extracts of Sambucus chinensis, and 3 of them were inhibited by Ophiopegon japonicas extracts and Reineckia camea extracts, with ratios of treatment to control large than 1.5 in all. Leaf extract of A. chinensis had significant anti-bacteria ability, and could be used as a potential plant source for bactericide. [Conclusion] The results laid a foundation for exploring active com- pounds and elucidating the mechanism in it.展开更多
目的观察吉祥草中甾体皂苷RCE-4对宫颈癌裸鼠移植瘤的抑制作用。方法采用传统方法分离鉴定RCE-4。将0.3 m L Caski(4×107/m L)细胞悬液接种于裸鼠右侧后腿背部皮下,建立宫颈癌移植瘤模型,然后按瘤体积大小随机分为模型组、紫杉醇(1...目的观察吉祥草中甾体皂苷RCE-4对宫颈癌裸鼠移植瘤的抑制作用。方法采用传统方法分离鉴定RCE-4。将0.3 m L Caski(4×107/m L)细胞悬液接种于裸鼠右侧后腿背部皮下,建立宫颈癌移植瘤模型,然后按瘤体积大小随机分为模型组、紫杉醇(10 mg/kg)和RCE-4(25、50、100 mg/kg)组,1次/d给药,连续4周,末次给药的次日处死裸鼠,称量裸鼠体质量、瘤质量,测量肿瘤体积,计算抑瘤率。光镜下观察移植瘤组织形态学变化,TUNEL染色测定细胞凋亡率,免疫组化检测肿瘤组织中COX-2表达,荧光定量PCR测定移植瘤组织中Bcl-2、Bax、Caspase-3和Caspase-9 mRNA表达,计算Bcl-2/Bax值,Western blot检测移植瘤组织中Survivin蛋白表达。结果 RCE-4(25、50、100 mg/kg)可明显降低宫颈癌裸鼠移植瘤的体积和瘤质量(P<0.05,P<0.01),上调Bax、Caspase-3和Caspase-9 mRNA表达,下调Bcl-2 mRNA表达和COX-2、Survivin蛋白表达及Bcl-2/Bax值(P<0.05,P<0.01)。结论RCE-4对宫颈癌裸鼠移植瘤的生长具有显著抑制作用,其机制可能与其上调Bax、Caspase-9、Caspase-3的表达和下调Survivin、Bcl-2、COX-2的表达有关。展开更多
基金National Natural Science Foundation of China(Gr ant No.81172943)
文摘Phytochemical investigations of the aerial parts of Reineckia carnea, collected in Yunnan Province of China, were conducted to explore new chemical constituents. A series of chromatographic and spectroscopic procedures were utilized on the aqueous solution after partitioned with ethyl acetate, which resulted in the separation of a new furostanol-type glycoside and four known compounds. The structures of the isolated compounds were elucidated on the basis of spectroscopic techniques（1D and 2D NMR, IR, HRESIMS） as 26-O-β-D-glucopyranosyl-（25S）-5β-furost-20（22）-en-1α,3β,26-triol-1-O-α-L-arabinopyranosyl-（1→2）-[α-Lrhamnopyranos-yl]-3-O-α-L-rhamnopyranoside（1）,（1β,3β,16β,22S）-cholest-5-en-1,3,16,22-tetrol-1,16-di-（β-D-glucopyranoside）（2）, diosgenin（3）, β-sitosterol（4）, ecdysterone（5）.
基金National Natural Science Foundation of China (Grant No.30973628)
文摘The aim of current study was to investigate the chemical components of the aerial part of Reineckia carnea,collected in Yunnan Province of China.Repeated column chromatography（CC）separations were performed to isolate and purify components. Compounds were identified by the analysis of their 1D and 2D NMR data as well as IR and MS spectra.A new pregnane-type glycoside,named 1α,3β-diol-5β-pregn-16-ene-20-one-1-O-α-L-arabinosyl-（1→2）-α-L-rhamnoside（1）,together withβ-amyrin（2）, stigmasterol（3）,α-spinasterol-3-O-β-D-glucoside（4）,naringenin（5）,β-sitosterol（6）and daucosterol（7）,were isolated from ethyl acetate（EtOAc）and normal butanol（n-BuOH）extracts.Compounds 2,4,5 were isolated from this plant for the first time.
基金supported by grants from the National Natural Science Foundation of China(No. 81001694)
文摘Two new isospirostanol sapogenins named （25R）-5/%spirostane-（1αα 3a）-diol （1） and （25R）-5/%spirostane-（1α, 2α, 3α, 4α）- tetrol （2） were isolated from the whole herb ofReineckia carnea （Andr.） Kunth. The structures of the sapogenins were elucidated by chemical and spectral methods. methods
文摘以苗族常用药材吉祥草(Reineckia carnea(Andr.)Kunth)为试材,采用盆栽控制试验,设置了对照(CK)、单独增二氧化碳(eC:CO_(2)浓度为(800±20)μmol·mol^(-1))、单独施氮(eN:施加5 g NH_(4)NO_(3))、同时增二氧化碳与施氮(eC+eN:CO_(2)浓度为(800±20)μmol·mol^(-1),且施加5 g NH_(4)NO_(3))4个处理,研究CO_(2)浓度升高、氮沉降及二者的交互作用对吉祥草的生长及其药用成分的影响,以期为吉祥草在药用生产上提供参考依据。结果表明:1)升高CO_(2)浓度和施氮或同时升高CO_(2)浓度和施氮均显著促进了吉祥草的形态生长,总生物量和可溶性蛋白质含量显著增加,茎中的碳含量显著降低,超氧化物歧化酶(SOD)活性无显著变化;2)施氮处理下全氮含量显著增加,过氧化氢酶(CAT)活性、根和叶中的皂苷含量显著下降,在此基础上升高CO_(2)浓度可缓解皂苷下降,CAT活性显著升高,内源激素含量在施氮或同时升高CO_(2)浓度和施氮处理下显著增加。综上所述,在实际生产过程中,可适当增加CO_(2)浓度,氮肥浓度不宜过高,有利于吉祥草的生长,并保证其药用品质不受损害。
基金Supported by National Natural Science Foundation(31200488,31370692)Surface Project of Natural Science Foundation of Hubei Province(2014CFB573)~~
文摘[Objective] This study was conducted to develop potential natural plant products for controlling walnut blight pathogen and other bacteria. [Method] Inhibitory effects of extracts obtained from 15 plants with 3 solvents on bacteria were investi- gated by disk diffusion method. [Results] Except the extracts from Magnolia grandi- flora and Typha orientalis, extracts of 13 plant leaves presented inhibitory effects on 5 bacteria strains to certain degrees. Among them, the effect of water extract of Aesculu schinensis on Bacillus sp. XHE8 was the strongest, with inhibition zone di- ameter reaching （31.3＋3.9） mm and the ratio to control above 5.0. Four of the 5 tested strains were sensitive to the extracts of Sambucus chinensis, and 3 of them were inhibited by Ophiopegon japonicas extracts and Reineckia camea extracts, with ratios of treatment to control large than 1.5 in all. Leaf extract of A. chinensis had significant anti-bacteria ability, and could be used as a potential plant source for bactericide. [Conclusion] The results laid a foundation for exploring active com- pounds and elucidating the mechanism in it.
