The process of bone remodelling plays an essential role in the emergence and maintenance of bone geometry and its internal structure.Osteoclasts are one of the three main bone cell types that play a crucial role in th...The process of bone remodelling plays an essential role in the emergence and maintenance of bone geometry and its internal structure.Osteoclasts are one of the three main bone cell types that play a crucial role in the bone remodelling cycle.At the microstructural level,osteoclasts create bone deficits by eroding resorption cavities.Understanding how these cavities impair the mechanical quality of the bone is not only relevant in quantifying the impact of resorption cavities in healthy bone and normal aging,but maybe even more so in quantifying their role in metabolic bone diseases.Meta-bolic bone diseases and their treatment are both known to affect the bone remodelling cycle;hence,the bone mechanical competence can and will be affected.How-ever,the current knowledge of the precise dimensions of these cavities and their effect on bone competence is rather limited.This is not surprising considering the difficulties in deriving three-dimensional(3D)properties from two-dimensional(2D)histological sections.The measurement difficulties are reflected in the evalua-tion of how resorption cavities affect bone competence.Although detailed 3D models are generally being used to quantify the mechanical impact of the cavities,the representation of the cavities themselves has basicallybeen limited to simplified shapes and averaged cavityproperties.Qualitatively,these models indicate that cav-ity size and location are important,and that the effectof cavities is larger than can be expected from simplebone loss.In summary,the dimensions of osteoclastresorption cavities were until recently estimated from2D measures;hence,a careful interpretation of resorp-tion cavity dimensions is necessary.More effort needsto go into correctly quantifying resorption cavities usingmodern 3D imaging techniques like micro-computedtomography(micro-CT)and synchrotron radiation CT.Osteoclast resorption cavities affect bone competence.The structure-function relationships have been ana-lysed using computational models that,on one hand,provide rather detailed information on trabecular bonestructure,but on the other incorporate rather crudeassumptions on cavity dimensions.The use of high-resolution representations and parametric descriptionscould be potential routes to improve the quantitativefidelity of these models.展开更多
BACKGROUND The bone remodeling during orthodontic treatment for malocclusion often requires a long duration of around two to three years,which also may lead to some complications such as alveolar bone resorption or to...BACKGROUND The bone remodeling during orthodontic treatment for malocclusion often requires a long duration of around two to three years,which also may lead to some complications such as alveolar bone resorption or tooth root resorption.Low-intensity pulsed ultrasound(LIPUS),a noninvasive physical therapy,has been shown to promote bone fracture healing.It is also reported that LIPUS could reduce the duration of orthodontic treatment;however,how LIPUS regulates the bone metabolism during the orthodontic treatment process is still unclear.AIM To investigate the effects of LIPUS on bone remodeling in an orthodontic tooth movement(OTM)model and explore the underlying mechanisms.METHODS A rat model of OTM was established,and alveolar bone remodeling and tooth movement rate were evaluated via micro-computed tomography and staining of tissue sections.In vitro,human bone marrow mesenchymal stem cells(hBMSCs)were isolated to detect their osteogenic differentiation potential under compression and LIPUS stimulation by quantitative reverse transcription-polymerase chain reaction,Western blot,alkaline phosphatase(ALP)staining,and Alizarin red staining.The expression of Yes-associated protein(YAP1),the actin cytoskeleton,and the Lamin A/C nucleoskeleton were detected with or without YAP1 small interfering RNA(siRNA)application via immunofluorescence.RESULTS The force treatment inhibited the osteogenic differentiation potential of hBMSCs;moreover,the expression of osteogenesis markers,such as type 1 collagen(COL1),runt-related transcription factor 2,ALP,and osteocalcin(OCN),decreased.LIPUS could rescue the osteogenic differentiation of hBMSCs with increased expression of osteogenic marker inhibited by force.Mechanically,the expression of LaminA/C,F-actin,and YAP1 was downregulated after force treatment,which could be rescued by LIPUS.Moreover,the osteogenic differentiation of hBMSCs increased by LIPUS could be attenuated by YAP siRNA treatment.Consistently,LIPUS increased alveolar bone density and decreased vertical bone absorption in vivo.The decreased expression of COL1,OCN,and YAP1 on the compression side of the alveolar bone was partially rescued by LIPUS.CONCLUSION LIPUS can accelerate tooth movement and reduce alveolar bone resorption by modulating the cytoskeleton-Lamin A/C-YAP axis,which may be a promising strategy to reduce the orthodontic treatment process.展开更多
The resorption of nutrients from senescent leaves allows plants to conserve and recycle nutrients. To explore the adaptation strategies of desert plants to nutrient-limited environments, we selected four typical deser...The resorption of nutrients from senescent leaves allows plants to conserve and recycle nutrients. To explore the adaptation strategies of desert plants to nutrient-limited environments, we selected four typical desert plants(Populus euphratica Oliv., Tamarix ramosissima Ledeb., Glycyrrhiza inflata Batal., and Alhagi camelorum Fisch.) growing in the desert area of the northern margin of the Tarim Basin,China. The contents of nitrogen(N), phosphorus(P), potassium(K), calcium(Ca), magnesium(Mg), and Ferrum(Fe) in the leaves of these four typical desert plants and their resorption characteristics were analyzed. The relationship of nutrient resorption efficiency with leaf functional traits and soil physical-chemical properties in two different habitats(saline-alkali land and sandy land) was discussed.The results showed that the four plants resorbed most of the elements. Ca was enriched in the leaves of P.euphratica, G. inflate, and A. camelorum;Mg was enriched in the leaves of G. inflata;and Fe was enriched in the leaves of the four plants. The results of the redundancy analysis showed that leaf thickness, soil electrical conductivity, and soil P content were the major factors affecting the nutrient resorption efficiency of the four plants. Leaf thickness was negatively correlated with N resorption efficiency(NRE),P resorption efficiency, and Fe resorption efficiency;soil electrical conductivity was positively correlated with the resorption efficiency of most elements;and soil P content was negatively correlated with the resorption efficiency of most elements in the plant leaves. The results showed that soil physical-chemical properties and soil nutrient contents had an important impact on the nutrient resorption of plant leaves.The same species growing in different habitats also differed in their resorption of different elements. The soil environment of plants and the biological characteristics of plant leaves affected the resorption of nutrient elements in different plants. The purpose of this study is to provide small-scale data support for the protection of ecosystems in nutrient-deficient areas by studying leaf functional strategies and nutrient conservation mechanisms of several typical desert plants.展开更多
Tooth root resorption is multifactorial, and its etiology and pathogenesis are poorly understood. Tooth root resorption is often incidentally revealed on radiographic examination. Here, we report a case of root resorp...Tooth root resorption is multifactorial, and its etiology and pathogenesis are poorly understood. Tooth root resorption is often incidentally revealed on radiographic examination. Here, we report a case of root resorption in multiple teeth of unknown etiology. Radiographic examination revealed root resorption in the cervical region of the left lateral incisor, canine, first and second premolars, and first and second molars of the mandible. Panoramic radiographs revealed no mandibular lesions that could cause tooth resorption. The patient did not wish to undergo any treatment and is currently under observation. Tooth root resorption with unknown etiology is rare, and further case collection is needed to determine the cause and treatment.展开更多
Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveo...Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveolar bone formation and mineralization,and periodontal function.Bone resorption is controlled by a complex molecular network,and BSP can promote osteoclast differentiation and bone resorption.It is also associated with the metastasis of a range of malignancies.Osteoclasts(OC)are thought to be the only cells involved in bone resorption and play an important role in bone formation and late developmental remodeling.Osteoporosis and periodontal disease are caused by excessive bone resorption.This article will summarize the osteoclasts differentiation,the biological function of bone resorption,and explore the progress of the prevention and treatment of the related bone resorption diseases such as osteoporosis and periodontal disease through the regulation of osteoclasts.展开更多
Problems due to underground cavities at the level of soft and polymorphic sandstones, collapses and infiltration of acid waters through the fracture networks have been observed around the Kakobola hydroelectric develo...Problems due to underground cavities at the level of soft and polymorphic sandstones, collapses and infiltration of acid waters through the fracture networks have been observed around the Kakobola hydroelectric development, constituting potential risks for the stability of the dam. The objective of this article aims to highlight the major cause that can explain the generating processes of these cavities. Indeed, to do this, the exploration of the subsoil was made possible thanks to the eleven (11) boreholes drilled on the site, the determination of cavities by the method of colored tracers and the petrographic characterization using a microscope. The latter, by means of thin sections, reveals the presence of quartz arenites. The mineralogical characterization of rock materials was carried out using X-ray fluorescence and X-ray diffraction methods. X-ray fluorescence revealed the presence of nickel, zinc and iron. Diffraction shows us an abundance of silica, mainly quartz and its metastable phases, in particular tridymite and cristobalite. It also made it possible to question the presence of carbonated minerals. The drillings enabled us to elaborate the logs and to bring out a geological model of the sector of study. These models were produced on the basis of drilling and observations on excavation. These data also reveal the presence of underground cavities which were also confirmed by the colored tracer method. These cavities would be of natural origin due to the presence of carbonate minerals observed in the rocks and which are attacked by acidic waters attested by the pH 5 measurements of the waters of the Lufuku River. The infiltration of water in rocky materials of low density and through the various networks of fractures and cavities leads to instability of the rock and could damage the hydroelectric development.展开更多
Introduction: One of the most common risks of fixed orthodontic therapy is the evidence of root resorption post orthodontic treatment ranging from mild root blunting to severe root resorption of significant portions o...Introduction: One of the most common risks of fixed orthodontic therapy is the evidence of root resorption post orthodontic treatment ranging from mild root blunting to severe root resorption of significant portions of the root structure. The etiology of apical root resorption is multifactorial but largely depends upon the type of orthodontic tooth movement and treatment duration. Study Objective: The primary aim of this study is to examine the association of external apical root resorption and vertical correction in anterior open bite malocclusions using panoramic radiographs for evaluation. Materials and Methods: Pre-treatment and post-treatment panoramic radiographs of 16 patients with anterior open bite malocclusions were scored to assess root resorption of anterior teeth (U/L 3-3). Initial and final root length and total tooth length for U/L 3-3 were measured on panoramic radiographs in Dolphin imaging. Results: Of the 24 pairs of measurements, 7 had a significant p-value (p Conclusions: A statistically significant amount of root resorption was observed, from pre-treatment to post-treatment radiographs. All mandibular anterior teeth except the mandibular right lateral incisors showed evidence of root resorption.展开更多
为分析空心弹高速入水的机理及其特性,基于雷诺时均Navier-Stokes方程、VOF(volume of fluid)多相流模型、Realizable k-ε湍流模型,引入Schnerr-Sauer空化模型和重叠网格技术对空心弹高速入水进行数值模拟研究,获得了通孔孔径和头部形...为分析空心弹高速入水的机理及其特性,基于雷诺时均Navier-Stokes方程、VOF(volume of fluid)多相流模型、Realizable k-ε湍流模型,引入Schnerr-Sauer空化模型和重叠网格技术对空心弹高速入水进行数值模拟研究,获得了通孔孔径和头部形状对空心弹的空化特性、空泡形态和入水运动特性的影响规律。研究显示数值计算的空泡形态和入水速度、位移曲线与实验结果吻合较好,验证了数值模拟方法的可行性。结果表明:当通孔孔径不同时,通孔孔径越大,空化现象越明显,通孔射流越长,但对空泡半径的影响不大;通孔孔径越小,空泡闭合时间越早,与水面碰撞产生的阻力系数峰值越高,空心弹入水稳定后其阻力系数也越大;无量纲直径在0.575~0.600之间时,空心弹的运动最为稳定。当头部锥角不同时,头部锥角越大,空泡直径越大,空化现象出现得越晚,但空化生成的速度更快;随着头部锥角的增大,阻力系数变大,空心弹的速度衰减变快,相同时间运动的距离较短;头部锥角越大,俯仰角的变化越小,空心弹的运动越稳定。展开更多
文摘The process of bone remodelling plays an essential role in the emergence and maintenance of bone geometry and its internal structure.Osteoclasts are one of the three main bone cell types that play a crucial role in the bone remodelling cycle.At the microstructural level,osteoclasts create bone deficits by eroding resorption cavities.Understanding how these cavities impair the mechanical quality of the bone is not only relevant in quantifying the impact of resorption cavities in healthy bone and normal aging,but maybe even more so in quantifying their role in metabolic bone diseases.Meta-bolic bone diseases and their treatment are both known to affect the bone remodelling cycle;hence,the bone mechanical competence can and will be affected.How-ever,the current knowledge of the precise dimensions of these cavities and their effect on bone competence is rather limited.This is not surprising considering the difficulties in deriving three-dimensional(3D)properties from two-dimensional(2D)histological sections.The measurement difficulties are reflected in the evalua-tion of how resorption cavities affect bone competence.Although detailed 3D models are generally being used to quantify the mechanical impact of the cavities,the representation of the cavities themselves has basicallybeen limited to simplified shapes and averaged cavityproperties.Qualitatively,these models indicate that cav-ity size and location are important,and that the effectof cavities is larger than can be expected from simplebone loss.In summary,the dimensions of osteoclastresorption cavities were until recently estimated from2D measures;hence,a careful interpretation of resorp-tion cavity dimensions is necessary.More effort needsto go into correctly quantifying resorption cavities usingmodern 3D imaging techniques like micro-computedtomography(micro-CT)and synchrotron radiation CT.Osteoclast resorption cavities affect bone competence.The structure-function relationships have been ana-lysed using computational models that,on one hand,provide rather detailed information on trabecular bonestructure,but on the other incorporate rather crudeassumptions on cavity dimensions.The use of high-resolution representations and parametric descriptionscould be potential routes to improve the quantitativefidelity of these models.
基金Supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China,No.2022YFA1105800the National Natural Science Foundation of China,No.81970940.
文摘BACKGROUND The bone remodeling during orthodontic treatment for malocclusion often requires a long duration of around two to three years,which also may lead to some complications such as alveolar bone resorption or tooth root resorption.Low-intensity pulsed ultrasound(LIPUS),a noninvasive physical therapy,has been shown to promote bone fracture healing.It is also reported that LIPUS could reduce the duration of orthodontic treatment;however,how LIPUS regulates the bone metabolism during the orthodontic treatment process is still unclear.AIM To investigate the effects of LIPUS on bone remodeling in an orthodontic tooth movement(OTM)model and explore the underlying mechanisms.METHODS A rat model of OTM was established,and alveolar bone remodeling and tooth movement rate were evaluated via micro-computed tomography and staining of tissue sections.In vitro,human bone marrow mesenchymal stem cells(hBMSCs)were isolated to detect their osteogenic differentiation potential under compression and LIPUS stimulation by quantitative reverse transcription-polymerase chain reaction,Western blot,alkaline phosphatase(ALP)staining,and Alizarin red staining.The expression of Yes-associated protein(YAP1),the actin cytoskeleton,and the Lamin A/C nucleoskeleton were detected with or without YAP1 small interfering RNA(siRNA)application via immunofluorescence.RESULTS The force treatment inhibited the osteogenic differentiation potential of hBMSCs;moreover,the expression of osteogenesis markers,such as type 1 collagen(COL1),runt-related transcription factor 2,ALP,and osteocalcin(OCN),decreased.LIPUS could rescue the osteogenic differentiation of hBMSCs with increased expression of osteogenic marker inhibited by force.Mechanically,the expression of LaminA/C,F-actin,and YAP1 was downregulated after force treatment,which could be rescued by LIPUS.Moreover,the osteogenic differentiation of hBMSCs increased by LIPUS could be attenuated by YAP siRNA treatment.Consistently,LIPUS increased alveolar bone density and decreased vertical bone absorption in vivo.The decreased expression of COL1,OCN,and YAP1 on the compression side of the alveolar bone was partially rescued by LIPUS.CONCLUSION LIPUS can accelerate tooth movement and reduce alveolar bone resorption by modulating the cytoskeleton-Lamin A/C-YAP axis,which may be a promising strategy to reduce the orthodontic treatment process.
基金supported by the National Natural Science Foundation of China (32001145)。
文摘The resorption of nutrients from senescent leaves allows plants to conserve and recycle nutrients. To explore the adaptation strategies of desert plants to nutrient-limited environments, we selected four typical desert plants(Populus euphratica Oliv., Tamarix ramosissima Ledeb., Glycyrrhiza inflata Batal., and Alhagi camelorum Fisch.) growing in the desert area of the northern margin of the Tarim Basin,China. The contents of nitrogen(N), phosphorus(P), potassium(K), calcium(Ca), magnesium(Mg), and Ferrum(Fe) in the leaves of these four typical desert plants and their resorption characteristics were analyzed. The relationship of nutrient resorption efficiency with leaf functional traits and soil physical-chemical properties in two different habitats(saline-alkali land and sandy land) was discussed.The results showed that the four plants resorbed most of the elements. Ca was enriched in the leaves of P.euphratica, G. inflate, and A. camelorum;Mg was enriched in the leaves of G. inflata;and Fe was enriched in the leaves of the four plants. The results of the redundancy analysis showed that leaf thickness, soil electrical conductivity, and soil P content were the major factors affecting the nutrient resorption efficiency of the four plants. Leaf thickness was negatively correlated with N resorption efficiency(NRE),P resorption efficiency, and Fe resorption efficiency;soil electrical conductivity was positively correlated with the resorption efficiency of most elements;and soil P content was negatively correlated with the resorption efficiency of most elements in the plant leaves. The results showed that soil physical-chemical properties and soil nutrient contents had an important impact on the nutrient resorption of plant leaves.The same species growing in different habitats also differed in their resorption of different elements. The soil environment of plants and the biological characteristics of plant leaves affected the resorption of nutrient elements in different plants. The purpose of this study is to provide small-scale data support for the protection of ecosystems in nutrient-deficient areas by studying leaf functional strategies and nutrient conservation mechanisms of several typical desert plants.
文摘Tooth root resorption is multifactorial, and its etiology and pathogenesis are poorly understood. Tooth root resorption is often incidentally revealed on radiographic examination. Here, we report a case of root resorption in multiple teeth of unknown etiology. Radiographic examination revealed root resorption in the cervical region of the left lateral incisor, canine, first and second premolars, and first and second molars of the mandible. Panoramic radiographs revealed no mandibular lesions that could cause tooth resorption. The patient did not wish to undergo any treatment and is currently under observation. Tooth root resorption with unknown etiology is rare, and further case collection is needed to determine the cause and treatment.
基金National Natural Science Foundation Project(No.81260275)。
文摘Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveolar bone formation and mineralization,and periodontal function.Bone resorption is controlled by a complex molecular network,and BSP can promote osteoclast differentiation and bone resorption.It is also associated with the metastasis of a range of malignancies.Osteoclasts(OC)are thought to be the only cells involved in bone resorption and play an important role in bone formation and late developmental remodeling.Osteoporosis and periodontal disease are caused by excessive bone resorption.This article will summarize the osteoclasts differentiation,the biological function of bone resorption,and explore the progress of the prevention and treatment of the related bone resorption diseases such as osteoporosis and periodontal disease through the regulation of osteoclasts.
文摘Problems due to underground cavities at the level of soft and polymorphic sandstones, collapses and infiltration of acid waters through the fracture networks have been observed around the Kakobola hydroelectric development, constituting potential risks for the stability of the dam. The objective of this article aims to highlight the major cause that can explain the generating processes of these cavities. Indeed, to do this, the exploration of the subsoil was made possible thanks to the eleven (11) boreholes drilled on the site, the determination of cavities by the method of colored tracers and the petrographic characterization using a microscope. The latter, by means of thin sections, reveals the presence of quartz arenites. The mineralogical characterization of rock materials was carried out using X-ray fluorescence and X-ray diffraction methods. X-ray fluorescence revealed the presence of nickel, zinc and iron. Diffraction shows us an abundance of silica, mainly quartz and its metastable phases, in particular tridymite and cristobalite. It also made it possible to question the presence of carbonated minerals. The drillings enabled us to elaborate the logs and to bring out a geological model of the sector of study. These models were produced on the basis of drilling and observations on excavation. These data also reveal the presence of underground cavities which were also confirmed by the colored tracer method. These cavities would be of natural origin due to the presence of carbonate minerals observed in the rocks and which are attacked by acidic waters attested by the pH 5 measurements of the waters of the Lufuku River. The infiltration of water in rocky materials of low density and through the various networks of fractures and cavities leads to instability of the rock and could damage the hydroelectric development.
文摘Introduction: One of the most common risks of fixed orthodontic therapy is the evidence of root resorption post orthodontic treatment ranging from mild root blunting to severe root resorption of significant portions of the root structure. The etiology of apical root resorption is multifactorial but largely depends upon the type of orthodontic tooth movement and treatment duration. Study Objective: The primary aim of this study is to examine the association of external apical root resorption and vertical correction in anterior open bite malocclusions using panoramic radiographs for evaluation. Materials and Methods: Pre-treatment and post-treatment panoramic radiographs of 16 patients with anterior open bite malocclusions were scored to assess root resorption of anterior teeth (U/L 3-3). Initial and final root length and total tooth length for U/L 3-3 were measured on panoramic radiographs in Dolphin imaging. Results: Of the 24 pairs of measurements, 7 had a significant p-value (p Conclusions: A statistically significant amount of root resorption was observed, from pre-treatment to post-treatment radiographs. All mandibular anterior teeth except the mandibular right lateral incisors showed evidence of root resorption.
文摘为分析空心弹高速入水的机理及其特性,基于雷诺时均Navier-Stokes方程、VOF(volume of fluid)多相流模型、Realizable k-ε湍流模型,引入Schnerr-Sauer空化模型和重叠网格技术对空心弹高速入水进行数值模拟研究,获得了通孔孔径和头部形状对空心弹的空化特性、空泡形态和入水运动特性的影响规律。研究显示数值计算的空泡形态和入水速度、位移曲线与实验结果吻合较好,验证了数值模拟方法的可行性。结果表明:当通孔孔径不同时,通孔孔径越大,空化现象越明显,通孔射流越长,但对空泡半径的影响不大;通孔孔径越小,空泡闭合时间越早,与水面碰撞产生的阻力系数峰值越高,空心弹入水稳定后其阻力系数也越大;无量纲直径在0.575~0.600之间时,空心弹的运动最为稳定。当头部锥角不同时,头部锥角越大,空泡直径越大,空化现象出现得越晚,但空化生成的速度更快;随着头部锥角的增大,阻力系数变大,空心弹的速度衰减变快,相同时间运动的距离较短;头部锥角越大,俯仰角的变化越小,空心弹的运动越稳定。
