Single-layer reticulated dome structure are commonly high-profile building in the public and can be attractive targets for terrorist bombings,so the public can benefit from enhanced safety with a stronger understandin...Single-layer reticulated dome structure are commonly high-profile building in the public and can be attractive targets for terrorist bombings,so the public can benefit from enhanced safety with a stronger understanding of the behavior of single-layer reticulated dome structure under explosion.This paper investigates the fluid-structure interaction process and the dynamic response performance of the singlelayer reticulated dome under external blast load.Both experimental and numerical results shown that structural deformation is remarkably delayed compared with the velocity of blast wave,which advises the dynamic response of large-span reticulated dome structure has a negligible effect on the blast wave propagation under explosion.Four failure modes are identified by comparing the plastic development of each ring and the residual spatial geometric of the structure,i.e.,minor vibration,local depression,severe damage,and overall collapse.The plastic deformation energy and the displacement potential energy of the structure are the main consumers of the blast energy.In addition,the stress performance of the vertex member and the deep plastic ratio of the whole structure can serve as qualitative indicators to distinguish different failure modes.展开更多
In platelets, most of the ADP is stored in dense granules and released into extracellular space through exocytosis as a signaling molecule upon platelet activation. Glycolysis and the TCA cycle consume considerable am...In platelets, most of the ADP is stored in dense granules and released into extracellular space through exocytosis as a signaling molecule upon platelet activation. Glycolysis and the TCA cycle consume considerable amounts of ADP;however, limiting quantities of available ADP to make ATP through OXPHOS result in failure of ATP production and release of energy as heat into the surroundings. Thus, body heat may be a potential product of circulating platelets. Furthermore, the incomplete OXPHOS process causes the production of ROS that leads to earlier platelet death resulting in shorter life span. In the future, this new function may have a wide variety of clinical applications.展开更多
T lymphocytes,the main participants of cellular immunity,can express a variety of surface molecules and form different lymphocyte subsets under the induction of different factors to play the functions of immune regula...T lymphocytes,the main participants of cellular immunity,can express a variety of surface molecules and form different lymphocyte subsets under the induction of different factors to play the functions of immune regulation and immune killing.Studies have shown that platelets play a crucial role in maintaining the stable differentiation of lymphocytes and the balance in immunomodulation.Therefore,it is necessary to study the effect of platelets on lymphocytes in vitro to better understand the role of platelets in the immune system and broaden the application of adoptive immunotherapy.Methods:Cell counting and microscopic observation were used to detect the effect of activated platelets on lymphocyte proliferation in vitro;Flow cytometry was used to detect whether changes in platelet activity affect the proportion of lymphocyte subpopulations in vitro,and to detect differences in the expression of granzyme B;lactate dehydrogenase assay(LDH)was used to determine the difference in lymphocyte killing activity caused by platelet activity in vitro.Results:This was the first to promote lymphocyte proliferation through the expression or release of certain molecules in vitro,demonstrating that platelet activation is one of the key factors.Secondly,activated platelets or inactivated platelets promoted lymphocyte subset differentiation by enhancing the proportion of CD3+CD8+T lymphocytes(CTL cells)but had a slight effect on the proportion of CD3+CD4+T(Th cells)and CD4+CD25+T lymphocytes(Treg cells).Then,it was found that either activated platelets or inactivated platelets down-regulated the proportion of natural killer(NK)T lymphocytes,while activated platelets significantly enhance the proportion of NK lymphocytes.Therefore,by further detecting the killing activity of PBMCs treated with platelets,it was found that activated platelets promoted the extensive anti-tumor activity of lymphocytes and significantly increased the expression of granzyme B.Conclusion:Our results suggest that activated platelets promote lymphocyte proliferation,optimize lymphocyte subpopulation ratio,and promote cytotoxic effect of lymphocytes in vitro,which may provide a new strategy for optimizing the adoptive immunotherapy culture system and improving its efficacy.展开更多
Objective:Explore the diagnostic value of total bile acids/platelets in HBV related liver fibrosis.Methods:160 patients with chronic HBV infection admitted to the Infection Department of the First Affiliated Hospital ...Objective:Explore the diagnostic value of total bile acids/platelets in HBV related liver fibrosis.Methods:160 patients with chronic HBV infection admitted to the Infection Department of the First Affiliated Hospital of Hainan Medical College from February 2021 to December 2022 were selected.They were divided into two groups based on the degree of liver fibrosis detected by liver biopsy:significant liver fibrosis group and non-significant liver fibrosis group.The total bile acid/blood platelet levels and their correlation with liver fibrosis in the two groups were compared and observed,and the efficacy of other non-invasive liver fibrosis diagnostic models was evaluated.Results:(1)Compared with the non-significant liver fibrosis group,the significant liver fibrosis group showed an increase in total bile acid levels,a decrease in platelet levels,and a significant increase in total bile acid/platelet levels(P<0.05).(2)Platelets decrease with the increase of liver fibrosis degree,total bile acids increase with the increase of liver fibrosis degree,and total bile acids/platelets increase with the increase of liver fibrosis degree.(3)The area under the curve(AUC)of total bile acid/platelet,APRI,FIB-4,and elastography in diagnosing the degree of liver fibrosis were 0.69,0.57,0.56,and 0.68,respectively.Conclusions:The diagnostic efficacy of total bile acids/platelets in diagnosing HBV related liver fibrosis is no less than that of other liver fibrosis diagnostic methods,and it is non-invasive,simple,and convenient,which is worthy of further clinical promotion and validation.展开更多
基金financial support from the China Postdoctora Science Foundation(project No.2021M690406)financial supports from the National Natural Science Foundation of China(project No.51708521,51778183)。
文摘Single-layer reticulated dome structure are commonly high-profile building in the public and can be attractive targets for terrorist bombings,so the public can benefit from enhanced safety with a stronger understanding of the behavior of single-layer reticulated dome structure under explosion.This paper investigates the fluid-structure interaction process and the dynamic response performance of the singlelayer reticulated dome under external blast load.Both experimental and numerical results shown that structural deformation is remarkably delayed compared with the velocity of blast wave,which advises the dynamic response of large-span reticulated dome structure has a negligible effect on the blast wave propagation under explosion.Four failure modes are identified by comparing the plastic development of each ring and the residual spatial geometric of the structure,i.e.,minor vibration,local depression,severe damage,and overall collapse.The plastic deformation energy and the displacement potential energy of the structure are the main consumers of the blast energy.In addition,the stress performance of the vertex member and the deep plastic ratio of the whole structure can serve as qualitative indicators to distinguish different failure modes.
文摘In platelets, most of the ADP is stored in dense granules and released into extracellular space through exocytosis as a signaling molecule upon platelet activation. Glycolysis and the TCA cycle consume considerable amounts of ADP;however, limiting quantities of available ADP to make ATP through OXPHOS result in failure of ATP production and release of energy as heat into the surroundings. Thus, body heat may be a potential product of circulating platelets. Furthermore, the incomplete OXPHOS process causes the production of ROS that leads to earlier platelet death resulting in shorter life span. In the future, this new function may have a wide variety of clinical applications.
文摘T lymphocytes,the main participants of cellular immunity,can express a variety of surface molecules and form different lymphocyte subsets under the induction of different factors to play the functions of immune regulation and immune killing.Studies have shown that platelets play a crucial role in maintaining the stable differentiation of lymphocytes and the balance in immunomodulation.Therefore,it is necessary to study the effect of platelets on lymphocytes in vitro to better understand the role of platelets in the immune system and broaden the application of adoptive immunotherapy.Methods:Cell counting and microscopic observation were used to detect the effect of activated platelets on lymphocyte proliferation in vitro;Flow cytometry was used to detect whether changes in platelet activity affect the proportion of lymphocyte subpopulations in vitro,and to detect differences in the expression of granzyme B;lactate dehydrogenase assay(LDH)was used to determine the difference in lymphocyte killing activity caused by platelet activity in vitro.Results:This was the first to promote lymphocyte proliferation through the expression or release of certain molecules in vitro,demonstrating that platelet activation is one of the key factors.Secondly,activated platelets or inactivated platelets promoted lymphocyte subset differentiation by enhancing the proportion of CD3+CD8+T lymphocytes(CTL cells)but had a slight effect on the proportion of CD3+CD4+T(Th cells)and CD4+CD25+T lymphocytes(Treg cells).Then,it was found that either activated platelets or inactivated platelets down-regulated the proportion of natural killer(NK)T lymphocytes,while activated platelets significantly enhance the proportion of NK lymphocytes.Therefore,by further detecting the killing activity of PBMCs treated with platelets,it was found that activated platelets promoted the extensive anti-tumor activity of lymphocytes and significantly increased the expression of granzyme B.Conclusion:Our results suggest that activated platelets promote lymphocyte proliferation,optimize lymphocyte subpopulation ratio,and promote cytotoxic effect of lymphocytes in vitro,which may provide a new strategy for optimizing the adoptive immunotherapy culture system and improving its efficacy.
基金Natural Science Foundation of Hainan Province (No.819MS122)Hainan Provincial Department of Education Fund Project (No.hnky2017-38)。
文摘Objective:Explore the diagnostic value of total bile acids/platelets in HBV related liver fibrosis.Methods:160 patients with chronic HBV infection admitted to the Infection Department of the First Affiliated Hospital of Hainan Medical College from February 2021 to December 2022 were selected.They were divided into two groups based on the degree of liver fibrosis detected by liver biopsy:significant liver fibrosis group and non-significant liver fibrosis group.The total bile acid/blood platelet levels and their correlation with liver fibrosis in the two groups were compared and observed,and the efficacy of other non-invasive liver fibrosis diagnostic models was evaluated.Results:(1)Compared with the non-significant liver fibrosis group,the significant liver fibrosis group showed an increase in total bile acid levels,a decrease in platelet levels,and a significant increase in total bile acid/platelet levels(P<0.05).(2)Platelets decrease with the increase of liver fibrosis degree,total bile acids increase with the increase of liver fibrosis degree,and total bile acids/platelets increase with the increase of liver fibrosis degree.(3)The area under the curve(AUC)of total bile acid/platelet,APRI,FIB-4,and elastography in diagnosing the degree of liver fibrosis were 0.69,0.57,0.56,and 0.68,respectively.Conclusions:The diagnostic efficacy of total bile acids/platelets in diagnosing HBV related liver fibrosis is no less than that of other liver fibrosis diagnostic methods,and it is non-invasive,simple,and convenient,which is worthy of further clinical promotion and validation.
