Objective:To present platelet large cell ratio(P-LCR),reticulocyte,and immature reticulocyte fraction(IRF)values as novel parameters in diagnosis and response to treatment in patients developing sacroiliitis.Methods:S...Objective:To present platelet large cell ratio(P-LCR),reticulocyte,and immature reticulocyte fraction(IRF)values as novel parameters in diagnosis and response to treatment in patients developing sacroiliitis.Methods:Sixty-eight patients with clinical symptoms and Brucella standard tube agglutination(Wright)or Brucella Coombs agglutination test titers≥1:160 were included in the study.Two groups were established,one developing sacroiliitis and another with no sacroiliitis development.P-LCR,reticulocyte,and IRF levels were measured using a Sysmex XN-9000 device(Japan).These were then compared between the two groups.Results:Reticulocyte(P=0.037)and IRF(P=0.026)levels were significantly lower among the patients developing sacroiliitis compared to the non-sacroiliitis group,while P-LCR(P=0.003)levels were significantly higher.P-LCR had the most powerful correlation with sacroiliitis development.Significant negative correlation was observed between reticulocyte,IRF levels and sacroiliitis.Conclusions:Elevated P-LCR levels were observed as a marker of persisting inflammation in patients developing sacroiliitis,while low reticulocyte and IRF levels secondary to bone marrow involvement were detected.These three parameters emerged as highly significant markers in terms of diagnosis and reflecting responses to treatment in organ involvement such as sacroiliitis in brucellosis.These are presented as inexpensive,and easily accessible novel parameters.展开更多
<strong>Background:</strong> Diagnosis of iron deficiency anemia with currently available tests is rendered difficult in hemodialysis patients. <strong>The aim:</strong> To investigate the role...<strong>Background:</strong> Diagnosis of iron deficiency anemia with currently available tests is rendered difficult in hemodialysis patients. <strong>The aim:</strong> To investigate the role of reticulocyte Hemoglobin Content (CHr) in the diagnosis of iron deficiency anemia in hemodialysis children in comparison to the used traditional markers and assess the impressiveness and the utility of Neutrophil Gelatinase Associated Lipocalin (NGAL) as a novel biomarker of iron status in those patients. <strong>Methods:</strong> This study investigated CHr in addition to NGAL serum level in the same line with traditional markers for anemia, including: CBC, serum iron, ferritin, total iron-binding capacity (TIBC), and transferrin saturation (TSAT%). <strong>Results:</strong> It is more significant that CHr content in hemodialysis children is lower than their controls as they are (27.06 ± 2.90) pg and (32.86 ± 3.59) pg, respectively, p = 0.01. There is no significant difference regarding NGAL between the study groups. Significant negative correlation between CHr with ferritin, urea, creatinine, and positively correlated with iron and RBCS. CHr showed a sensitivity of 90% and specificity of 86.67% to detect iron-deficiency anemia with a cut-off value of 27 pg. <strong>Conclusion:</strong> CHr is superior to ferritin and TSAT % for the early diagnosis of iron deficiency anemia in hemodialysis children;our results do not support NGAL as a marker of anemia in hemodialysis patients.展开更多
Using the method of phenylhydrazine-induced anemia in rabbits, a mass of new reticulocytes, which synchronously grow, were got in vivo. The measurements of deformation index, orientation index, electrophoresis mobilit...Using the method of phenylhydrazine-induced anemia in rabbits, a mass of new reticulocytes, which synchronously grow, were got in vivo. The measurements of deformation index, orientation index, electrophoresis mobility etc. were performed for more than 72 h in the process of reticulocytes turning into red blood cells in vivo. There were obvious changes in the micro- rheological characteristics of reticulocytes in the course of turning into erythrocytes. The present study is significant in clinic for studying erythrocytes' microrheological characteristics when there are a lot of reticulocytes in blood, and also important in basic theorem for studying reticulocytes microrheological characteristics. It makes up a deficiency in the study on microrheological characteristics of reticulocytes turning into new RBCs from reticulocytes during reticulocytes life span.展开更多
I.INTRODUCTION Cell proliferation and differentiation are modulated by various regulators. It is well documented that loss control of any one of the key steps in the regulation of normal cell proliferation would cause...I.INTRODUCTION Cell proliferation and differentiation are modulated by various regulators. It is well documented that loss control of any one of the key steps in the regulation of normal cell proliferation would cause abnormal cell growth even result in malignant transformation.Currently, besides the well-known growth factor which is of positive regulation for cell proliferation, growth inhibitors with negative effect on cell growth are also isolated from vari-展开更多
Pre-menopausal women are at risk for iron deficiencydue to menstrual blood losses. The prevalence rates(PR) of iron depletion, iron deficiency anemia (IDA), andiron deficiency were 34.4%, 15.1% and 49.5% inpre-menopau...Pre-menopausal women are at risk for iron deficiencydue to menstrual blood losses. The prevalence rates(PR) of iron depletion, iron deficiency anemia (IDA), andiron deficiency were 34.4%, 15.1% and 49.5% inpre-menopausal non-pregnant women respectively inChina. Traditionally, the diagnosis of iron deficiencyrelies on the hematological markers (hemoglobin (HGB),mean corpuscular volume (MCV), mean corpuscularhemoglobin (MCH), red cell distribution width (RDW))and biochemical markers (serum ferritin (SF), serum iron(SI), transferrin saturation (TS), total iron-bindingcapacity (TIBC)). Recently, the reticulocyte展开更多
By applying the technique of cybridization between reticulocytes and myeloma cells, we reported that the erythroid cell cytoplasms had some regulatory effects upon the malignancy of the myeloma. Though such a method p...By applying the technique of cybridization between reticulocytes and myeloma cells, we reported that the erythroid cell cytoplasms had some regulatory effects upon the malignancy of the myeloma. Though such a method produces reliable results, to establish inducible cytogenetic mutations in myeloma cells, to select the HGPRT^- mutants and finally to select the cybrids through HAT medium, however, still need a lot of time. Recently, by using gene transfer techniques, new selectable genetic markers could be introduced into cells directly, this provides an alternative new way to make cellular hybrids. Yet up to now, the host cells for gene transfer are all the kind of cells with nuclei. Here, by展开更多
文摘Objective:To present platelet large cell ratio(P-LCR),reticulocyte,and immature reticulocyte fraction(IRF)values as novel parameters in diagnosis and response to treatment in patients developing sacroiliitis.Methods:Sixty-eight patients with clinical symptoms and Brucella standard tube agglutination(Wright)or Brucella Coombs agglutination test titers≥1:160 were included in the study.Two groups were established,one developing sacroiliitis and another with no sacroiliitis development.P-LCR,reticulocyte,and IRF levels were measured using a Sysmex XN-9000 device(Japan).These were then compared between the two groups.Results:Reticulocyte(P=0.037)and IRF(P=0.026)levels were significantly lower among the patients developing sacroiliitis compared to the non-sacroiliitis group,while P-LCR(P=0.003)levels were significantly higher.P-LCR had the most powerful correlation with sacroiliitis development.Significant negative correlation was observed between reticulocyte,IRF levels and sacroiliitis.Conclusions:Elevated P-LCR levels were observed as a marker of persisting inflammation in patients developing sacroiliitis,while low reticulocyte and IRF levels secondary to bone marrow involvement were detected.These three parameters emerged as highly significant markers in terms of diagnosis and reflecting responses to treatment in organ involvement such as sacroiliitis in brucellosis.These are presented as inexpensive,and easily accessible novel parameters.
文摘<strong>Background:</strong> Diagnosis of iron deficiency anemia with currently available tests is rendered difficult in hemodialysis patients. <strong>The aim:</strong> To investigate the role of reticulocyte Hemoglobin Content (CHr) in the diagnosis of iron deficiency anemia in hemodialysis children in comparison to the used traditional markers and assess the impressiveness and the utility of Neutrophil Gelatinase Associated Lipocalin (NGAL) as a novel biomarker of iron status in those patients. <strong>Methods:</strong> This study investigated CHr in addition to NGAL serum level in the same line with traditional markers for anemia, including: CBC, serum iron, ferritin, total iron-binding capacity (TIBC), and transferrin saturation (TSAT%). <strong>Results:</strong> It is more significant that CHr content in hemodialysis children is lower than their controls as they are (27.06 ± 2.90) pg and (32.86 ± 3.59) pg, respectively, p = 0.01. There is no significant difference regarding NGAL between the study groups. Significant negative correlation between CHr with ferritin, urea, creatinine, and positively correlated with iron and RBCS. CHr showed a sensitivity of 90% and specificity of 86.67% to detect iron-deficiency anemia with a cut-off value of 27 pg. <strong>Conclusion:</strong> CHr is superior to ferritin and TSAT % for the early diagnosis of iron deficiency anemia in hemodialysis children;our results do not support NGAL as a marker of anemia in hemodialysis patients.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39770203) Doctoral Foundation of China (Grant No. 9729).
文摘Using the method of phenylhydrazine-induced anemia in rabbits, a mass of new reticulocytes, which synchronously grow, were got in vivo. The measurements of deformation index, orientation index, electrophoresis mobility etc. were performed for more than 72 h in the process of reticulocytes turning into red blood cells in vivo. There were obvious changes in the micro- rheological characteristics of reticulocytes in the course of turning into erythrocytes. The present study is significant in clinic for studying erythrocytes' microrheological characteristics when there are a lot of reticulocytes in blood, and also important in basic theorem for studying reticulocytes microrheological characteristics. It makes up a deficiency in the study on microrheological characteristics of reticulocytes turning into new RBCs from reticulocytes during reticulocytes life span.
文摘I.INTRODUCTION Cell proliferation and differentiation are modulated by various regulators. It is well documented that loss control of any one of the key steps in the regulation of normal cell proliferation would cause abnormal cell growth even result in malignant transformation.Currently, besides the well-known growth factor which is of positive regulation for cell proliferation, growth inhibitors with negative effect on cell growth are also isolated from vari-
文摘Pre-menopausal women are at risk for iron deficiencydue to menstrual blood losses. The prevalence rates(PR) of iron depletion, iron deficiency anemia (IDA), andiron deficiency were 34.4%, 15.1% and 49.5% inpre-menopausal non-pregnant women respectively inChina. Traditionally, the diagnosis of iron deficiencyrelies on the hematological markers (hemoglobin (HGB),mean corpuscular volume (MCV), mean corpuscularhemoglobin (MCH), red cell distribution width (RDW))and biochemical markers (serum ferritin (SF), serum iron(SI), transferrin saturation (TS), total iron-bindingcapacity (TIBC)). Recently, the reticulocyte
文摘By applying the technique of cybridization between reticulocytes and myeloma cells, we reported that the erythroid cell cytoplasms had some regulatory effects upon the malignancy of the myeloma. Though such a method produces reliable results, to establish inducible cytogenetic mutations in myeloma cells, to select the HGPRT^- mutants and finally to select the cybrids through HAT medium, however, still need a lot of time. Recently, by using gene transfer techniques, new selectable genetic markers could be introduced into cells directly, this provides an alternative new way to make cellular hybrids. Yet up to now, the host cells for gene transfer are all the kind of cells with nuclei. Here, by
