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Delineation of biomarkers and molecular pathways of residual effects of fluoxetine treatment in juvenile rhesus monkeys by proteomic profiling 被引量:1
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作者 Yu Yan Dong Ik Park +2 位作者 Anja Horn Mari Golub Christoph W.Turck 《Zoological Research》 SCIE CAS CSCD 2023年第1期30-42,共13页
Fluoxetine(Prozac^(TM))is the only antidepressant approved by the US Food and Drug Administration(FDA)for the treatment of major depressive disorder(MDD)in children.Despite its considerable efficacy as a selective ser... Fluoxetine(Prozac^(TM))is the only antidepressant approved by the US Food and Drug Administration(FDA)for the treatment of major depressive disorder(MDD)in children.Despite its considerable efficacy as a selective serotonin reuptake inhibitor,the possible long-term effects of fluoxetine on brain development in children are poorly understood.In the current study,we aimed to delineate molecular mechanisms and protein biomarkers in the brains of juvenile rhesus macaques(Macaca mulatta)one year after the discontinuation of fluoxetine treatment using proteomic and phosphoproteomic profiling.We identified several differences in protein expression and phosphorylation in the dorsolateral prefrontal cortex(DLPFC)and cingulate cortex(CC)that correlated with impulsivity in animals,suggesting that the GABAergic synapse pathway may be affected by fluoxetine treatment.Biomarkers in combination with the identified pathways contribute to a better understanding of the mechanisms underlying the chronic effects of fluoxetine after discontinuation in children. 展开更多
关键词 Major depressive disorder FLUOXETINE rhesus monkeys PROTEOMICS GABAergic synapse
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Reproductive traits of polycystic ovary syndrome in female rhesus monkeys 被引量:1
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作者 唐向辉 曹跃龄 +1 位作者 杨泽星 赵富鲜 《Zoological Research》 CAS CSCD 北大核心 2012年第1期37-42,共6页
The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome(PCOS),which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS fe... The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome(PCOS),which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS female monkeys.Six adult female rhesus monkeys aged 6-10 a,were divided into a PCOS group and a control group.The PCOS group were given two cycles of subcutaneous injections of propionic acid testosterone(PAT),3.5 mg/kg body weight,on day 1,day 3,and day 5 of the menstrual cycle,respectively,and then given muscle injections of human chorionic gonadotropin(HCG),350 IU/kg body wtight,on day 7,day 9,and day 11,respectively.Results showed that high levels of serum LH and T [(5.35±0.17) IU/L and(7.58±0.14) ng/mL,respectively],and a high ratio value of LH/FSH(5.35/1.30=4.12) were observed in the PCOS group.No significant differences were found in serum FSH,E2,and P in the PCOS group compared with those of the control.Polycystic ovaries in the PCOS monkeys were recorded by live ultrasound.The blastocysts rates of the PCOS vs.the control were 23.53% vs.66.67%,and there was a significant difference between the two groups.This study shows that PAT coupled with HCG can induce PCOS in rhesus monkeys in the short term.The reproductive features of PCOS monkeys were similar to those of PCOS patients. 展开更多
关键词 PCOS rhesus monkey REPRODUCTION Controlled ovary stimulation
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F-heparin modified intraocular lenses in Rhesus monkeys 被引量:2
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作者 Gui-Qin Wang Han-Qing Gu +2 位作者 Jia-Qin Yuan Hui-Min Sun and Yan-Shan Xu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2010年第2期141-144,共4页
AIMIn order to improve the biocompatibility of intraocular lenses (IOL), the polymethylmethacrylate (PMMA) IOL was modified with F-heparin.
关键词 intraocular lenses F-heparin surface modification BIOCOMPATIBILITY rhesus monkeys
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Expression of insulin-like growth factor-1 mRNA and protein level of corpora striata in ischemic side at the early stage of middle cerebral artery ischemia/reperfusion in rhesus monkeys
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作者 Huanmin Gao Rui Zhang Yunliang Guo 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第2期133-136,共4页
BACKGROUND: Insulin-like growth factor-I(IGF-1), as one of the important members of growth factor family, participants in the regulation of many physiological functions and behaviors, having very strong neuroprotec... BACKGROUND: Insulin-like growth factor-I(IGF-1), as one of the important members of growth factor family, participants in the regulation of many physiological functions and behaviors, having very strong neuroprotective effect. However, the expression of IGF-1 following cerebral ischemia/reperfusion is still disputed. OBJECTIVE: To observe the expression of IGF-1 and protein of corpora striata in ischemic side at the early stage of middle cerebral artery ischemia/reperfusion in rhesus monkey. DESIGN : A completely randomized grouping design, controlled animal experiment SETTING : Institute of Cerebrovascular Disease, Affiliated Hospital of Medical College of Qingdao University MATERIALS: ① Totally 17 rhesus monkeys , of either gender, aged 4 to 5 years, were enrolled . Seven rhesus monkeys observed with gene chip were randomly divided into 2 groups: sham operation group (n=3) and ischemia/reperfusion group 〈n=4〉. Ten rhesus monkeys observed with in situ hybridization and immunohistochemistry method were randomly divided into 2 groups: sham operation group 〈n=3 〉and ischemia/reperfusion group (n=7). Rhesus monkeys observed under microscope were divided into 2 groups: sham operation group (n=6) and ischamia/reperfusion group (n=-11).②Materials used in the experiment: cresyl violet (Sigma Company, America); immunohistochemical reagent kit ( Huamei Bio-engineering Company); In situ hybridization reagent kit (Boshide Bio-engineering Co.Ltd, Wuhan); 12 800 dots chip (Boxing Company, Shanghai). METHODS : This experiment was carried out at the Institute of Cerebrovascular Disease, Affiliated Hospital of Medical College of Qingdao University from January 2001 to December 2003.① The onset area of middle cerebral artery was blocked for 2 hours, middle cerebral artery ischemia/reperfusion models were created.② After ischemia/reperfusion for 24 hours, cerebral tissue sections of rhesus monkeys were prepared and stained with cresyl violet. Image analysis was performed with 5001W image analysis software. Morphological change of corpora striata of operative side was observed in the rhesus monkeys between two groups. Total RNA was extracted from cerebral tissue. ③ Detection of gene chip: Cy3-duTP and Cy5-duTP were used to respectively perform reverse transcription labeling. The sample was reversely transcribed into cDNA, then hybridized with cDNA of cerebral tissue. Genes with the separate absolute value of cy3 and cy5〉800, cY3/cy5 〉 2(high expression) or 〈 0.5 (low expression) were found out. Those were genes with differential expression. ④ The expressions of IGF-1 mRNA and protein level of corpora striata in ischemic side of rhe- sus monkeys were detected between sham operation group and ischemia/reperfusion group at 9 and 24 hours after ischemia/reperfusion with in situ hybridization method and immunohistochemical method. Brown granules were IGF-1 protein positive cells. ⑤ Analysis of variance was used in the difference comparison of measurement data among groups. MAIN OUTCOME MEASURES : ① Change of morphological structure of corpora striata at ischemic side in rhesus monkeys. ② Change of cerebral gene expression profiles at ischemia/reperfusion in rhesus monkeys between two groups.③ Expression of IGF-1 mRNA and protein level of corpora striata at ischemia/reperfu- sion in rhesus monkeys between two groups. RESULTS : ① Pathological change : Obvious pathological change of cerebral infarction appeared in the ischemia and reperfusion group, while there was no such pathological change in the sham operation group.② Change of gene expression profile : There were 4480 genes with difference expression in the ischemia/reperfusion group and sham-operation group, in which, 260 genes had high expression and their absolute value was over 800, and 63 genes had low expression, cy3/cy5 of IGF-1 was 0.379, being relative low ex- pression. ③ IGF-1 mRNA and protein positive cell counts in corpora striata at cerebral ischemic side[IGF-1 mRNA: 〈9.72±1.18),(9.11 ±0.76),(14.77±0.60) counts/field:lGF-1 protein: (15.11 ±1.83),(15.39±0.78), (34.62±0.97)counts/field, P 〈 0.05-0.01]. CONCLUSION: IGF-1 mRNA and protein are lowly expressed in middle cerebral artery of rhesus monkeys at ischemia/reperfusion. 展开更多
关键词 IG Expression of insulin-like growth factor-1 mRNA and protein level of corpora striata in ischemic side at the early stage of middle cerebral artery ischemia/reperfusion in rhesus monkeys MRNA
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Pharmacokinetics of recombinant human parathyroid hormone after subcutaneous administration in Rhesus monkeys by immunoradiometric assay
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作者 宋雪伟 陈知航 +4 位作者 车津晶 单成启 侯禹男 郑仁玖 程远国 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第2期118-121,共4页
The purpose of this research was to study the pharmacokinetics and the bioavailability of recombinant human parathyroid hormone [rhPTH (1-34)] in Rhesus monkeys after single and multiple subcutaneous administration.... The purpose of this research was to study the pharmacokinetics and the bioavailability of recombinant human parathyroid hormone [rhPTH (1-34)] in Rhesus monkeys after single and multiple subcutaneous administration. An immunoradiometric assay (IRMA) was used to determine the plasma drug concentration of rhFFH (1-34) after giving single dose of 10, 20 and 40 ug/kg and daily dose of 40 ug/kg for 7 d by subcutaneous administration, and intravenous injection of 20 ug/kg in Rhesus monkeys. The pharmacokinetic parameters were calculated by noncompartmental analysis. The drug plasma level quantitation range was from 0.027 to 2.22 ng/mL. The intra- and inter-assay precision (CV) of analysis were less than 15%, and the average recovery was about 93.0% ± 8.6% - 116.5% ± 14.0%. After subcutaneous administration of rhPTH(1-34) at dose of 10, 20 and 40 ug/kg, the average Tmax was 0.67, 0.5 and 0.83 h, Cmax were 1.85 ± 0.05, 3.23 ± 0.25 and 7.15 ± 1.19 ng/mL, the AUC(0-∞) were 3.4 ± 0.6, 10.7 ± 1.3 and 12.6 ± 1.5 ng/h/mL, and terminal-phase elimination T1/2 were 0.72 ± 0.10, 1.15 ± 0.10 and 1.03 ± 0.06 h, respectively. The absolute bioavailability of rhPTH (1-34) was 46.96% after subcutaneous administration of 20 ug/kg. There was no evidence of accumulation during systemic exposure of rhPTH (1-34) upon multiple dosing in Rhesus monkeys. The IRMA assay method provide reasonable sensitivity and specificity for the pharrnacokinetic study of rhPTH (1-34) after subcutaneous or intravenous administration in Rhesus monkeys. The pharmacokinetic characteristic of rhPTH (1-34) in monkeys shows linear relationship with the dose administered subcutaneously. 展开更多
关键词 RhPTH (1-34) PHARMACOKINETIC IRMA BIOAVAILABILITY rhesus monkey
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Activation of extracellular signal-related kinases 1 and 2 in Sertoli cells in experimentally cryptorchid rhesus monkeys 被引量:6
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作者 Xue-Sen Zhang Zhi-Hong Zhang Shu-Hua Guo Wei Yang Zhu-Qiang Zhang Jin-Xiang Yuan Xuan Jin Zhao-Yuan Hu Yi-Xun Liu 《Asian Journal of Andrology》 SCIE CAS CSCD 2006年第3期265-272,共8页
Aim: To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), c-Jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinases (MAPK) in respon... Aim: To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2 (ERK1/ 2), c-Jun N-terminal kinases (JNK) and p38 mitogen-activated protein kinases (MAPK) in response to heat stress in the cryptorchid testis, and to investigate a possible relation to Sertoli cell dedifferentiation. Methods: Immunohistochemistry and western blot were used to examine the expression and activation of ERK1/2, p38 and JNK in the cryptorchid testis at various stages after experimental cryptorchidism. Results: The abdominal temperature did not obviously change the total ERK1/2 expression but significantly activated phospho-ERK1/2 in the Sertoli cells of the cryptorchid testis. Heat stress increased total JNK expression in the Sertoli cells of the cryptorchid testis but did not activate phospho-JNK. Neither total p38 nor phospho-p38 was induced by heat stress in the Sertoli cells of the cryptorchid testis. Changes in the spatiotemporal expression of cytokeratin 18 (CK18), a marker of immature or undifferentiated Sertoli cells, were induced in the cryptorchid testis in a pattern similar to the activation of ERK1/2. Condusion: The activation of ERK1/2 in the testis may be related to dedifferentiation of Sertoli cells under heat stress induced by experimental cryptorchidism. 展开更多
关键词 rhesus monkey CRYPTORCHIDISM Sertoli cell DEDIFFERENTIATION extracellular signal-regulated kinases 1 and 2
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Excitatory amino acid changes in the brains of rhesus monkeys following selective cerebral deep hypothermia and blood flow occlusion 被引量:1
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作者 Jun Pu Xiaoqun Niu Jizong Zhao 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第2期143-148,共6页
Selective cerebral deep hypothermia and blood flow occlusion can enhance brain tolerance to ischemia and hypoxia and reduce cardiopulmonary complications in monkeys. Excitotoxicity induced by the release of a large am... Selective cerebral deep hypothermia and blood flow occlusion can enhance brain tolerance to ischemia and hypoxia and reduce cardiopulmonary complications in monkeys. Excitotoxicity induced by the release of a large amount of excitatory amino acids after cerebral ischemia is the major mechanism underlying ischemic brain injury and nerve cell death. In the present study, we used selective cerebral deep hypothermia and blood flow occlusion to block the bilateral common carotid arteries and/or bilateral vertebral arteries in rhesus monkey, followed by reperfusion using Ringer's solution at 4~C. Microdialysis and transmission electron microscope results showed that selective cerebral deep hypothermia and blood flow occlusion inhibited the release of glutamic acid into the extracellular fluid in the brain frontal lobe and relieved pathological injury in terms of the ultrastructure of brain tissues after severe cerebral ischemia. These findings indicate that cerebral deep hypothermia and blood flow occlusion can inhibit cytotoxic effects and attenuate ischemic/ hypoxic brain injury through decreasing the release of excitatory amino acids, such as glutamic acid. 展开更多
关键词 neural regeneration brain injury selective deep hypothermia MICRODIALYSIS rhesus monkey glutamic acid excitatory amino acids brain protection high performance liquid chromatogram ultrastructure grants-supported paper photographs-containing paper neuroregeneration
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Sidiming attenuates morphine withdrawal syndrome and nitric oxide (synthase) levels in morphine-dependent rats and rhesus monkeys
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作者 Zheng Yang Renbin Huang Jianchun Huang Shijun Zhang Xing Lin Yang Jiao Weizhe Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第33期2589-2593,共5页
The present study analyzed the effects of Sidiming, a Chinese herbal compound, on withdrawal syndrome, body weight loss, and serum levels of nitric oxide and its synthase in morphine- dependent rats and rhesus monkeys... The present study analyzed the effects of Sidiming, a Chinese herbal compound, on withdrawal syndrome, body weight loss, and serum levels of nitric oxide and its synthase in morphine- dependent rats and rhesus monkeys. These effects were compared with clonidine, an active control drug used for clinical treatment. Results showed that 4 and 8 g/kg Sidiming, respectively, significantly suppressed morphine withdrawal syndrome and reduced body mass loss in morphine-dependent rats. In addition, 2.4 and 4.8 g/kg Sidiming, respectively, significantly attenuated withdrawal syndrome in rhesus monkeys. High-dose Sidiming (8 g/kg in rats and 4.8 g/kg in rhesus monkeys) led to significantly inhibited serum levels of nitric oxide and its synthase in morphine-dependent rats and rhesus monkeys, which were greater than clonidine. These findings suggested that Sidiming treatment attenuated withdrawal syndrome in morphine-dependent rats and rhesus monkeys by inhibiting serum nitric oxide and its synthase. 展开更多
关键词 morphine dependence nitric oxide nitric oxide synthase OPIOID rhesus monkey Sidiming withdrawal syndrome
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THE ESOPHAGEAL CARCINOMA OF RHESUS MONKEYS FROM TAIHANG MOUNTAIN AREA OF HIGH MORBIDITY OF ESOPHAGEAL CARCINOMA
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作者 张红绪 仉怀林 +2 位作者 朱东明 赵晓进 侯进怀 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1993年第2期31-34,共4页
Taihang Mountain area, a high Incidence area of human esophageal cancer in Northern China. 124 Rhesus monkeys (Macacs Mulatta) were caught in the area in January 1989. Among them, two monkeys died of esophageal carcin... Taihang Mountain area, a high Incidence area of human esophageal cancer in Northern China. 124 Rhesus monkeys (Macacs Mulatta) were caught in the area in January 1989. Among them, two monkeys died of esophageal carcinoma in 1990. Case 1, a male monkey about 6. 5 yean old and weighing 14. 5 kg, had symptoms of salivation, vomiting and dysphagia in Fabruary 1990. The symptoms became gradually more serious and died in march 1990. Postmortem examination revealed a huge tumor in the distal segment of esophagus, causing severe structure of the organ. The tumor was classified as medullary type and histopathologically diagnosed as a well differentiated squamous cell carcinoma, with metastases to mediastinum and lymph nodes of right gastric group. Case 2, a female monkey ablut 11-year-old and weighing 10. 0 kg, showed loss of appetite, tiredness, somnolence, caughing and vomiting in September and died in December 1990. Autopsy revealed an annular tumor involving the whole clcumference of lower portion of the esophagus. The tumor was of ulcerative type and diagnosed as a well differentiated squamous cell carcinoma. The symptoms and pathological changes of the two monkeys showed high similarity to esophageal cancer in humen. We believe that the present findings would provide important leads for further study to clarify the etiology and pathogenesis ofhuman esophageal cancer in this high Incidence area of esophageal cancer.of 展开更多
关键词 Esophageal carcinoma EPIDEMIOLOGY rhesus monkey AETIOLOGY
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Artificial Nursing Procedure Establishment for Infant Rhesus Monkeys
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作者 Wang Hong Si Wei +1 位作者 Zhou Yin Chen Lixian 《Animal Husbandry and Feed Science》 CAS 2015年第6期381-382,388,共3页
Rhesus monkey can not achieve natural delivery due to various reasons,and cesarean section becomes an important midwifery to get infant monkeys. After caesarean section,the pregnant monkey is weak and postoperative wo... Rhesus monkey can not achieve natural delivery due to various reasons,and cesarean section becomes an important midwifery to get infant monkeys. After caesarean section,the pregnant monkey is weak and postoperative wound pain,so it can not personally feed infant monkeys which must be artificially fed. Thus,establishing suitable feeding management program is very important for improving survival rate of infant rhesus monkey and maintaining good health. We summarized food preparation method for infant rhesus monkeys as well as temperature setting and light control,and established the nursing program for newborn infant monkey and daily management process for infant monkeys. 展开更多
关键词 rhesus monkey Infant monkey nursing Feeding management
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Activation of extracellular signal-related kinases 1 and 2 in Sertoli cells in experimentally cryptorchid rhesus monkeys 被引量:1
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作者 Xue-Sen Zhang~+ Zhi-Hong Zhang~+ Shu-Hua Guo Wei Yang,Zhu-Qiang Zhang Jin-Xiang Yuan Xuan Jin Zhao-Yuan Hu Yi-Xun Liu State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,25 Bei Si Huan Road West,Beijing 100081,China 《Asian Journal of Andrology》 SCIE CAS CSCD 2006年第A03期265-272,385,共5页
Aim:To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2(ERK1/ 2),c-Jun N-terminal kinases(JNK)and p38 mitogen-activated protein kinases(MAPK)in response to heat str... Aim:To assess the spatiotemporal changes in the expression of extracellular signal-regulated kinases 1 and 2(ERK1/ 2),c-Jun N-terminal kinases(JNK)and p38 mitogen-activated protein kinases(MAPK)in response to heat stress in the cryptorchid testis,and to investigate a possible relation to Sertoli cell dedifferentiation.Methods:Immunohis- tochemistry and western blot were used to examine the expression and activation of ERK1/2,p38 and JNK in the cryptorchid testis at various stages after experimental cryptorchidism.Results:The abdominal temperature did not obviously change the total ERK1/2 expression but significantly activated phospho-ERK1/2 in the Sertoli cells of the cryptorchid testis.Heat stress increased total JNK expression in the Sertoli cells of the cryptorchid testis but did not activate phospho-JNK.Neither total p38 nor phospho-p38 was induced by heat stress in the Sertoli cells of the cryptorchid testis.Changes in the spatiotemporal expression of cytokeratin 18(CK18),a marker of immature or undifferentiated Sertoli cells,were induced in the cryptorchid testis in a pattern similar to the activation of ERK1/2. Conclusion:The activation of ERK1/2 in the testis may be related to dedifferentiation of Sertoli cells under heat stress induced by experimental cryptorchidism. 展开更多
关键词 rhesus monkey CRYPTORCHIDISM Sertoli cell DEDIFFERENTIATION extracellular signal-regulated kinases 1 and 2
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Characteristics of blood chemistry, hematology, and lymphocyte subsets in pregnant rhesus monkeys 被引量:1
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作者 WU Ming-Ling GONG Li +2 位作者 QIAN Can LIANG Zhi-Gang ZENG Wen 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2015年第6期409-414,共6页
The present study was designed to characterize the blood chemistry, hematology, and lymphocyte subsets in pregnant rhesus monkeys and provide baseline parameters for future studies of reproductive and developmental to... The present study was designed to characterize the blood chemistry, hematology, and lymphocyte subsets in pregnant rhesus monkeys and provide baseline parameters for future studies of reproductive and developmental toxicity and developmental immunotoxicity. Harem-mating was used in 96 female and 16 male rhesus monkeys. Pregnancy was confirmed on gestation day(GD)18 by ultrasound. The blood samples of rhesus monkeys were collected at various times(20 days before pregnancy and GD20, 100 and 150). The analyses of blood chemistry, hematology, and lymphocyte subsets were performed. Copmpared with 20 days before pregnancy, Significant decreases(P < 0.05) were observed in HCT and RBC on GD20, GD150 and in HGB on GD150, Significant increases in NEUT and decreases in LYMPH on GD20 were observed. Significant decreases in ALB from GD20 to GD150 were observed, significant decreases in TP was observed on GD100. Significant increases in mean GLU were observed on GD20 and GD150 during pregnancy. Significant decreases(P < 0.05) in CD20+ subsets on GD100, GD150 and CD4+/CD8+ ratio on GD150 were observed, The significant changes of MCV, MCHC, RDW-SD, MCV, MONO, ALT, AST, GLB, ALP, TBIL, DBIL, IBIL, GGT, CR-S, URIC, TC, TG and CK were observed during the pregnant period, but no biologic change were observed, There were no significant changes in MCH, RDW-CV, MPV, BUN, CD3+, CD4+ and CD8+ during pregnancy. These data provide a database for preclinical study in rhesus monkeys. Physiological anemia, hyperglycemia, and immune suppression may occur in pregnant rhesus monkey which is similar to that found in human, and it is essential to distinguish the physiological changes from the pharmacological effects in reproductive and developmental toxicity and developmental immunotoxicity studies of pharmaceuticals. 展开更多
关键词 Pregnant rhesus monkeys HEMATOLOGY Blood chemistry Lymphocyte subsets
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What interests them in the pictures? — Differences in eyetracking between rhesus monkeys and humans 被引量:2
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作者 Ying-Zhou Hu Hui-Hui Jiang +11 位作者 Ci-Rong Liu Jian-Hong Wang Cheng-Yang Yu Synnve Carlson Shang-Chuan Yang Veli-Matti Saarinen Joshua D Rizak Xiao-Guang Tian Hen Tan Zhu-Yue Chen Yuan-Ye Ma Xin-Tian Hu 《Neuroscience Bulletin》 SCIE CAS CSCD 2013年第5期553-564,共12页
Studies estimating eye movements have demonstrated that non-human primates have fixation patterns similar to humans at the first sight of a picture.In the current study,three sets of pictures containing monkeys,humans... Studies estimating eye movements have demonstrated that non-human primates have fixation patterns similar to humans at the first sight of a picture.In the current study,three sets of pictures containing monkeys,humans or both were presented to rhesus monkeys and humans.The eye movements on these pictures by the two species were recorded using a Tobii eye-tracking system.We found that monkeys paid more attention to the head and body in pictures containing monkeys,whereas both monkeys and humans paid more attention to the head in pictures containing humans.The humans always concentrated on the eyes and head in all the pictures,indicating the social role of facial cues in society.Although humans paid more attention to the hands than monkeys,both monkeys and humans were interested in the hands and what was being done with them in the pictures.This may suggest the importance and necessity of hands for survival.Finally,monkeys scored lower in eye-tracking when fixating on the pictures,as if they were less interested in looking at the screen than humans.The locations of fixation in monkeys may provide insight into the role of eye movements in an evolutionary context. 展开更多
关键词 eye-tracking rhesus monkey fixation gaze
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Interactive effects of morphine and dopaminergic compounds on spatial working memory in rhesus monkeys
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作者 Jian-Hong Wang Joshua Dominie Rizak +3 位作者 Yan-Mei Chen Liang Li Xin-Tian Hu Yuan-Ye Ma 《Neuroscience Bulletin》 SCIE CAS CSCD 2013年第1期37-46,共10页
Opiates and dopamine (DA) play key roles in learning and memory in humans and animals. Although interactions between these neurotransmitters have been found, their functional roles remain to be fully elucidated, and... Opiates and dopamine (DA) play key roles in learning and memory in humans and animals. Although interactions between these neurotransmitters have been found, their functional roles remain to be fully elucidated, and their dysfunction may contribute to human diseases and addiction. Here we investigated the interactions of morphine and dopaminergic neurotransmitter systems with respect to learning and memory in rhesus monkeys by using the Wisconsin General Test Apparatus (WGTA) delayed-response task. Morphine and DA agonists (SKF-38393, apomorphine and bromocriptine) or DA antagonists (SKF-83566, haloperidol and sulpiride) were co-administered to the monkeys 30 min prior to the task. We found that dose-patterned co-administration of morphine with D1 or D2 antagonists or agonists reversed the impaired spatial working memory induced by morphine or the compounds alone. For example, morphine at 0.01 mg/kg impaired spatial working memory, while morphine (0.01 mg/kg) and apomorphine (0.01 or 0.06 mg/kg) co-treatment ameliorated this effect. Our findings suggest that the interactions between morphine and dopaminergic compounds influence spatial working memory in rhesus monkeys. A better understanding of these interactive relationships may provide insights into human addiction. 展开更多
关键词 working memory MORPHINE DOPAMINE rhesus monkey
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Pharmacokinetics of recombinant human interleukin-3 in rhesus monkeys
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作者 汤仲明 刘秀文 屠敏 《Science China(Life Sciences)》 SCIE CAS 1997年第5期546-553,共8页
Concentration-time profiles of ^(125)I-labeled recombinant human interleukin-3 (125IrhIL-3) were de-termined by reverse phase high performance liquid chromatography (RHPLC) after intravenous and subcutaneous ad-minist... Concentration-time profiles of ^(125)I-labeled recombinant human interleukin-3 (125IrhIL-3) were de-termined by reverse phase high performance liquid chromatography (RHPLC) after intravenous and subcutaneous ad-ministration of the drug in 16 rhesus monkeys. The initial and terminal T1/2 in plasma after intravenous of 30 μg/kg were (0. 15 ±0.13) and (2. 21 ± 0. 59) h, respectively. Terminal half-lives after 30, 90 and 180μg/kg subcutaneous (s.c.) injections were 2. 0-3.8 h. Area under concentration-time curves (AUC) following s. c. were roughly in-creased with dose, while CL5 were similar among different dosages. The absorption rates were dependent on concentra-tion at injected site. Bioavailability was about 0.7 after s.c. Rapid biodegradation was found in plasma. Distribution profiles of total radioactivity were as follows: the highest level was found in urinary system; levels in bile-enteric sys-tem, lymph nodes, bone marrow and spleen were near to that in plasma, and level in brain was the lowest. The RH-PLC analysis revealed that kidney was one of the major organs for biodegradation. 展开更多
关键词 recombinant human interleukin-3 rhesus monkey PHARMACOKINETICS ^(125)l-labeling high perfor-mace liquid chromatography.
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STUDY ON THE PRECANCEROUS ESOPHAGEAL LESIONSOF RHESUS MONKEYS FROM TAIHANG AREAOF HIGH MORBIDITY OF ESOPHAGEAL
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作者 张红绪 赵晓进 +5 位作者 侯进怀 千卫星 吕锡太 仉怀林 朱东明 徐存栓 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1996年第1期5-8,共4页
Taihang Mountain Area is a high incidence area of esophageal cancer in Northern China Among 124 Rhesus monkeys caught in the area, two cases of esophageal carcinoma were observed in 1990, and their symptoms and pathol... Taihang Mountain Area is a high incidence area of esophageal cancer in Northern China Among 124 Rhesus monkeys caught in the area, two cases of esophageal carcinoma were observed in 1990, and their symptoms and pathological morphological structures showed similarity to a well differentiated squamous carcinoma in human. At the same time, the cytological examinations were made on the 89 Rhesus monkeys of different sexes and ages, and the cases for normal (N), light proliferation(LP), high proliferation I(HP Ⅰ) and high proliferation Ⅱ (HP Ⅱ) were 27(30%), 35(39%), 18(21%) and 9(10%),respectively. The proliferation of epithelium of esophagus in Rhesus monkeys changed with increasing age.The average age of the LP was 5.5 years old with the youngest aged 2 and the eldest aged 10; The average age of the HP I was 7.3 with the youngest aged 2.5 and the eldest over 10. The average age of the HP Ⅱ was 8.1 with the youngest aged 4.5 and the eldest over 10. In all cases,the highest incidence was at the age of 6-10. It peaked at 10 and then decreased with years. The ecological environment(diet, water and soil) for Rhesus monkeys from the Taihang Mountain Area is the same as that for Iocal residents in that area. In the region of a high incidence of human esophageal cancer, the morbidity of proliferation of esophageal epithelium and esophageal cancer was high not only for human but also for animals. And this indicated that the aetiology is closely connected with environment. 展开更多
关键词 Precancerous lesions Epidemiology rhesus monkey Aetiology.
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Notch signaling dependent differentiation of cholangiocyte-like cells from rhesus monkey embryonic stem cells 被引量:1
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作者 金立方 纪少珲 +1 位作者 杨纪峰 季维智 《Zoological Research》 CAS CSCD 北大核心 2011年第4期391-395,共5页
Rhesus monkey embryonic stem(rES) cells have similar characteristics to human ES cells,and might be useful as a substitute model for preclinical research.Notch signaling is involved in the formation of bile ducts,wh... Rhesus monkey embryonic stem(rES) cells have similar characteristics to human ES cells,and might be useful as a substitute model for preclinical research.Notch signaling is involved in the formation of bile ducts,which are composed of cholangiocytes.However,little is known about the role of Notch signaling in cholangiocytic commitment of ES cells.We analyzed the effect of Notch signaling on the induction of cholangiocyte-like cells from rES cells.About 80% of definitive endoderm(DE) cells were generated from rES cells after treatment with activin A.After treatment with BMP4 and FGF1 on matrigel coated wells in serum-free medium,rES-derived DE gave rise to cholangiocyte-like cells by expression of cholangiocytic specific proteins(CK7,CK18,CK19,CK20,and OV-6) and genes(GSTPi,IB4,and HNF1β).At the same time,expression of Notch 1 and Notch 2 mRNA were detected during cell differentiation,as well as their downstream target genes such as Hes 1 and Hes 5.Inhibition of the Notch signal pathway by L-685458 resulted in decreased expression of Notch and their downstream genes.In addition,the proportion of cholangiocyte-like cells declined from ~90% to ~20%.These results suggest that Notch signaling may play a critical role in cholangiocytic development from ES cells. 展开更多
关键词 rhesus monkey Embryonic stem cells CHOLANGIOCYTES Notch signaling
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A more consistent intraluminal rhesus monkey model of ischemic stroke 被引量:6
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作者 Bo Zhao Guowei Shang +11 位作者 Jian Chen Xiaokun Geng Xin Ye Guoxun Xu Ju Wang Jiasheng Zheng Hongjun Li Fauzia Akbary Shengli Li Jing Lu Feng Ling Xunming Ji 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第23期2087-2094,共8页
Endovascular surgery is advantageous in experimentally induced ischemic stroke because it causes fewer cranial traumatic lesions than invasive surgery and can closely mimic the pathophysiology in stroke patients. Howe... Endovascular surgery is advantageous in experimentally induced ischemic stroke because it causes fewer cranial traumatic lesions than invasive surgery and can closely mimic the pathophysiology in stroke patients. However, the outcomes are highly variable, which limits the accuracy of evaluations of ischemic stroke studies. In this study, eight healthy adult rhesus monkeys were randomized into two groups with four monkeys in each group: middle cerebral artery occlusion at origin segment (M1) and middle cerebral artery occlusion at M2 segment. The blood flow in the middle cerebral artery was blocked completely for 2 hours using the endovascular microcoil placement technique (1 mm × 10 cm) (undetachable), to establish a model of cerebral ischemia. The microcoil was withdrawn and the middle cerebral artery blood flow was restored. A reversible middle cerebral artery occlusion model was identified by hematoxylin-eosin staining, digital subtraction angiography, magnetic resonance angiography, magnetic resonance imaging, and neurological evaluation. The results showed that the middle cerebral artery occlusion model was successfully established in eight adult healthy rhesus monkeys, and ischemic lesions were apparent in the brain tissue of rhesus monkeys at 24 hours after occlusion. The rhesus monkeys had symptoms of neurological deficits. Compared with the M1 occlusion group, the M2 occlusion group had lower infarction volume and higher neurological scores. These experimental findings indicate that reversible middle cerebral artery occlusion can be produced with the endovascular microcoil technique in rhesus monkeys. The M2 occluded model had less infarction and less neurological impairment, which offers the potential for application in the field of brain injury research. 展开更多
关键词 nerve regeneration brain injury rhesus monkeys model middle cerebral artery MICROCOIL INFARCTION stroke interventional therapy digital subtraction angiography magnetic resonance image NEUROIMAGING NEUROREGENERATION
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A simple model of radial nerve injury in the rhesus monkey to evaluate peripheral nerve repair 被引量:3
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作者 Dong Wang Xijun Huang +7 位作者 Guo Fu Liqiang Gu Xiaolin Liu Honggang Wang Jun Hu Jianhua Yi Xiaofeng Niu Qingtang Zhu 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第10期1041-1046,共6页
Current research on bone marrow stem cell transplantation and autologous or xenogenic nerve transplantation for peripheral nerve regeneration has mainly focused on the repair of peripher-al nerve defects in rodents. I... Current research on bone marrow stem cell transplantation and autologous or xenogenic nerve transplantation for peripheral nerve regeneration has mainly focused on the repair of peripher-al nerve defects in rodents. In this study, we established a standardized experimental model of radial nerve defects in primates and evaluated the effect of repair on peripheral nerve injury. We repaired 2.5-cm lesions in the radial nerve of rhesus monkeys by transplantation of autografts, acellular allografts, or acellular allografts seeded with autologous bone marrow stem cells. Five months after surgery, regenerated nerve tissue was assessed for function, electrophysiology, and histomorphometry. Postoperative functional recovery was evaluated by the wrist-extension test. Compared with the simple autografts, the acellular allografts and allografts seeded with bone marrow stem cells facilitated remarkable recovery of the wrist-extension functions in the rhesus monkeys. This functional improvement was coupled with radial nerve distal axon growth, a higher percentage of neuron survival, increased nerve fiber density and diameter, increased myelin sheath thickness, and increased nerve conduction velocities and peak amplitudes of compound motor action potentials. Furthermore, the quality of nerve regeneration in the bone marrow stem cells-laden allografts group was comparable to that achieved with autografts. The wrist-extension test is a simple behavioral method for objective quantification of peripheral nerve regeneration. 展开更多
关键词 nerve regeneration peripheral nerve injury rhesus monkeys bone marrow stem cells allogeneic nerve TRANSPLANTATION wrist-extension test ELECTROPHYSIOLOGY neurological function NSFC grant neural regeneration
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Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells
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作者 陈栋梁 李荣荣 +5 位作者 张敬 卢斌 魏强 王淑芬 谢云华 季维智 《Zoological Research》 CAS CSCD 北大核心 2009年第4期345-353,共9页
In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts (MESFs), clonally derived fibroblasts from the MESFs (CMESFs), monkey oviductal fibroblasts (MOFs), monkey follicular granulo... In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts (MESFs), clonally derived fibroblasts from the MESFs (CMESFs), monkey oviductal fibroblasts (MOFs), monkey follicular granulosa fibroblast-like (MFGs) cells, monkey follicular granulosa epithelium-like (MFGEs) cells, were developed for the maintenance of rhesus embryonic stem cells (rESCs). We found that MESFs, CMESFs, MOFs and MFGs, but not MFGEs, support the growth of rhesus embryonic stem cells. Moreover, we detected some genes that are upregulated in supportive feeder cell lines by semi-quantitative PCR. In the present study, we applied the GeneChip Rhesus Macaque Genome Array of Affymetrix Corporation to study the expression profiles of these five feeder cell lines, in purpose to find out which cytokines and signaling pathways were important in maintaining the rESCs, mRNAs of eight genes, including GREM2, bFGF, KITLG, DKK3, GREM1, AREG, SERPINF1 and LTBP1, were found to be upregulated in supportive feeder cell lines, but not in MFGE. The results indicate that many signaling pathways may play redundant roles in supporting the undifferentiated growth and maintenance of pluripotency in rESCs. 展开更多
关键词 Embryonic stem cells rhesus monkey feeders SELF-RENEWAL PLURIPOTENCY Expression profile Signaling pathway
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