目的利用基因芯片技术研究“益气调血,扶本培元”针法对快速老化小鼠SAMP10皮质基因表达的影响。方法选取雄性8月龄快速老化小鼠SAMP10(分成针刺腧穴组,对照组)和同源正常老化小鼠SAMR1,采用cDNA m icroarray技术,分析伴随快速老化SAMP1...目的利用基因芯片技术研究“益气调血,扶本培元”针法对快速老化小鼠SAMP10皮质基因表达的影响。方法选取雄性8月龄快速老化小鼠SAMP10(分成针刺腧穴组,对照组)和同源正常老化小鼠SAMR1,采用cDNA m icroarray技术,分析伴随快速老化SAMP10皮质基因表达谱的变化以及针刺对其的影响。结果芯片结果显示,伴快速老化,P10皮质共有9类17个基因的表达发生变化,15个基因表达下调,2个基因表达上调,施予“益气调血,扶本培元”针法后,所有与P10快速老化相关基因的表达均被部分或全部的逆转,显示针刺具有特异性。结论“益气调血,扶本培元”针法能够良性调节脑老化相关基因的表达,并可能通过该种作用延缓脑衰老。展开更多
Objective: To explore the mechanism of acupuncture for delaying aging. Methods: Using the senescence accelerated mouse pattern SAMP10 and the normal aging mice SAMR1 as models and applying RT-PCR and digoxin (DIG)-lab...Objective: To explore the mechanism of acupuncture for delaying aging. Methods: Using the senescence accelerated mouse pattern SAMP10 and the normal aging mice SAMR1 as models and applying RT-PCR and digoxin (DIG)-labeled Northern blot technique to observe expressions of NF-E2, YB-1, LRG47 genes in the forebrain, cortex and hippocampus in a 8-month old SAMR1 control group, a 8-month old SAMP10 control group, a 8-month old SAMP10 acupuncture group and a 8-month old SAMP10 non-point stimulation group. Results: In the SAMP10 control group, the expressions of NF-E2, YB-1 and LRG47 were down-regulated in the forebrain, cortex and hippocampus, and after acupuncture they were up-regulated and tended to normal. Conclusion: The brain aging of the SAMP10 mice is related with abnormal expressions of NF-E2, YB-1 and LRG47 genes; and acupuncture can regulate the expressions of NF-E2, YB-1 and LRG47 genes, strengthening the functions of erythrocyte series, increasing the proliferation of cells and enhancing the cellular immune function in anti-bacteria, hence delaying aging.展开更多
文摘目的利用基因芯片技术研究“益气调血,扶本培元”针法对快速老化小鼠SAMP10皮质基因表达的影响。方法选取雄性8月龄快速老化小鼠SAMP10(分成针刺腧穴组,对照组)和同源正常老化小鼠SAMR1,采用cDNA m icroarray技术,分析伴随快速老化SAMP10皮质基因表达谱的变化以及针刺对其的影响。结果芯片结果显示,伴快速老化,P10皮质共有9类17个基因的表达发生变化,15个基因表达下调,2个基因表达上调,施予“益气调血,扶本培元”针法后,所有与P10快速老化相关基因的表达均被部分或全部的逆转,显示针刺具有特异性。结论“益气调血,扶本培元”针法能够良性调节脑老化相关基因的表达,并可能通过该种作用延缓脑衰老。
文摘Objective: To explore the mechanism of acupuncture for delaying aging. Methods: Using the senescence accelerated mouse pattern SAMP10 and the normal aging mice SAMR1 as models and applying RT-PCR and digoxin (DIG)-labeled Northern blot technique to observe expressions of NF-E2, YB-1, LRG47 genes in the forebrain, cortex and hippocampus in a 8-month old SAMR1 control group, a 8-month old SAMP10 control group, a 8-month old SAMP10 acupuncture group and a 8-month old SAMP10 non-point stimulation group. Results: In the SAMP10 control group, the expressions of NF-E2, YB-1 and LRG47 were down-regulated in the forebrain, cortex and hippocampus, and after acupuncture they were up-regulated and tended to normal. Conclusion: The brain aging of the SAMP10 mice is related with abnormal expressions of NF-E2, YB-1 and LRG47 genes; and acupuncture can regulate the expressions of NF-E2, YB-1 and LRG47 genes, strengthening the functions of erythrocyte series, increasing the proliferation of cells and enhancing the cellular immune function in anti-bacteria, hence delaying aging.
