In this study a novel microwave-assisted extraction(MAE) device was presented. In this newly developed system, a 3/4 wavelength microwave resonance cavity was used as the microwave coupling device for replacing a trad...In this study a novel microwave-assisted extraction(MAE) device was presented. In this newly developed system, a 3/4 wavelength microwave resonance cavity was used as the microwave coupling device for replacing a traditional microwave oven. This device is used to extract flavonoids from Radix et caulis acanthopanacis senticosi. The effects of solvent concentration, microwave forward power and solvent uptake rate on the extraction yield of flavonoids were investigated by using orthogonal experimental design. The results show that the effects of the solvent(ethanol) concentration and solvent uptake rate on the extraction yield of flavonoids are very significant, the effect of the interaction of solvent concentration and solvent uptake rate is significant and the effects of the other factors are general. Under the optimal conditions of MAE the extraction yield of flavonoids by this system is 46% higher than that by Soxhlet method. The RSD of the method was lower than 6%.展开更多
目的:研究不同浓度的刺五加水提物体外对弱精子症患者精子运动的影响,探讨其可能的作用机制。方法:将35例弱精子症患者经手淫法获得并通过上游优化处理的精子,与不同浓度刺五加水提物共同孵育30、60、120、180 m in后,应用计算机辅助精...目的:研究不同浓度的刺五加水提物体外对弱精子症患者精子运动的影响,探讨其可能的作用机制。方法:将35例弱精子症患者经手淫法获得并通过上游优化处理的精子,与不同浓度刺五加水提物共同孵育30、60、120、180 m in后,应用计算机辅助精子分析系统(CASA)观察不同浓度(2.5、5、10、20 g/L)刺五加水提物对人精子各运动参数的作用。结果:不同浓度刺五加水提物能明显提高弱精子症患者精子的运动能力,在浓度为5 g/L和10 g/L时,精子活率(MOT)、前向运动精子百分率、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径速度(VAP)与空白对照组相比,差异均有显著性(P<0.05)。结论:刺五加水提物在体外能明显改善弱精子症患者精子运动能力,其最佳浓度为10 g/L。展开更多
文摘In this study a novel microwave-assisted extraction(MAE) device was presented. In this newly developed system, a 3/4 wavelength microwave resonance cavity was used as the microwave coupling device for replacing a traditional microwave oven. This device is used to extract flavonoids from Radix et caulis acanthopanacis senticosi. The effects of solvent concentration, microwave forward power and solvent uptake rate on the extraction yield of flavonoids were investigated by using orthogonal experimental design. The results show that the effects of the solvent(ethanol) concentration and solvent uptake rate on the extraction yield of flavonoids are very significant, the effect of the interaction of solvent concentration and solvent uptake rate is significant and the effects of the other factors are general. Under the optimal conditions of MAE the extraction yield of flavonoids by this system is 46% higher than that by Soxhlet method. The RSD of the method was lower than 6%.
文摘目的研究黄芪和刺五加水提物对弱精子症患者体外精子运动活力的影响。方法将30例弱精子症患者的精子经上游优化法处理后分为8份,其中两份分别加入刺五加水提物和黄芪水提物,使其终浓度分别为10和20mg.mL-1;另取5份将10 mg.mL-1刺五加水提物和20 mg.mL-1黄芪水提物按体积比为4:1,2:1,1:1,1:2和1:4加入;另外1份加入Ham’s F10培养液作对照。分别于孵育15,30和60 m in后应用计算机辅助的精子分析系统(CASA)观察精子各运动参数。结果刺五加水提物和黄芪水提物联用能不同程度地提高弱精子症患者精子的体外运动能力。加入刺五加和黄芪水提物后,精子活率(Mot)、前向运动精子百分率[(A+B)%]、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径速度(VAP)均较对照组显著提高(均P<0.05)。10 mg.mL-1刺五加水提物和20 mg.mL-1黄芪水提物体积比为1:1,共同孵育60 m in作用效果最明显。结论刺五加和黄芪水提物联用能明显改善弱精子症患者精子的体外运动能力。
