目的比较正常妊娠和子痫前期胎盘上SMARCA5的表达,初步探讨SMARCA5与子痫前期的关系。方法选取2015年8月至2016年8月在海南省人民医院剖宫产分娩的正常晚孕妇女30例、轻度子痫前期孕妇21例及重度子痫前期孕妇18例。分别应用实时荧光定量...目的比较正常妊娠和子痫前期胎盘上SMARCA5的表达,初步探讨SMARCA5与子痫前期的关系。方法选取2015年8月至2016年8月在海南省人民医院剖宫产分娩的正常晚孕妇女30例、轻度子痫前期孕妇21例及重度子痫前期孕妇18例。分别应用实时荧光定量-PCR(q RT-PCR)和免疫组化法检测正常妊娠和子痫前期胎盘SMARCA5 m RNA和蛋白的表达水平。结果 q RT-PCR结果显示,正常妊娠组、轻度子痫前期组和重度子痫前期组胎盘SMARCA5 m RNA表达水平分别为(1.05±0.21)、(0.60±0.06)和(0.38±0.01),轻度子痫前期组与正常妊娠组比较显著下降,并且重度子痫前期组与轻度子痫前期组比较也显著下降,差异均有统计学意义(P<0.01);免疫组化结果同样显示,SMARCA5蛋白在子痫前期胎盘表达下降。结论子痫前期胎盘组织中SMARCA5表达明显下降,提示SMARCA5可能参与子痫前期的发病。展开更多
Gene transcription and new protein synthesis regulated by epigenetics play integral roles in the formation of new memories.However,as an important part of epigenetics,the function of chromatin remodeling in learning a...Gene transcription and new protein synthesis regulated by epigenetics play integral roles in the formation of new memories.However,as an important part of epigenetics,the function of chromatin remodeling in learning and memory has been less studied.Here,we showed that SMARCA5(SWI/SNF related,matrix-associated,actin-dependent regulator of chromatin,subfamily A,member 5),a critical chromatin remodeler,was responsible for hippocampus-dependent memory maintenance and neurogenesis.Using proteomics analysis,we found protein expression changes in the hippocampal dentate gyrus(DG)after the knockdown of SMARCA5 during contextual fear conditioning(CFC)memory maintenance in mice.Moreover,SMARCA5 was revealed to participate in CFC memory maintenance via modulating the proteins of metabolic pathways such as nucleoside diphosphate kinase-3(NME3)and aminoacylase 1(ACY1).This work is the first to describe the role of SMARCA5 in memory maintenance and to demonstrate the involvement of metabolic pathways regulated by SMARCA5 in learning and memory.展开更多
文摘目的比较正常妊娠和子痫前期胎盘上SMARCA5的表达,初步探讨SMARCA5与子痫前期的关系。方法选取2015年8月至2016年8月在海南省人民医院剖宫产分娩的正常晚孕妇女30例、轻度子痫前期孕妇21例及重度子痫前期孕妇18例。分别应用实时荧光定量-PCR(q RT-PCR)和免疫组化法检测正常妊娠和子痫前期胎盘SMARCA5 m RNA和蛋白的表达水平。结果 q RT-PCR结果显示,正常妊娠组、轻度子痫前期组和重度子痫前期组胎盘SMARCA5 m RNA表达水平分别为(1.05±0.21)、(0.60±0.06)和(0.38±0.01),轻度子痫前期组与正常妊娠组比较显著下降,并且重度子痫前期组与轻度子痫前期组比较也显著下降,差异均有统计学意义(P<0.01);免疫组化结果同样显示,SMARCA5蛋白在子痫前期胎盘表达下降。结论子痫前期胎盘组织中SMARCA5表达明显下降,提示SMARCA5可能参与子痫前期的发病。
基金supported by the Youth Program of the National Natural Science Foundation of China(32000788)Shandong Province Natural Science Foundation(ZR2019 BC097)+5 种基金Key Project from the National Natural Science Foundation of China(81830035)the Major program of National Natural Science Foundation of China(82090033)the Major Basic research program of Shandong Province Natural Science Foundation(ZR2019ZD35)The Major program of Technological innovation 2030"Brain science and brain-inspired research"(2021ZD0203002)Shandong Taishan Scholar Awardthe Fundamental Research Funds of Qingdao University.
文摘Gene transcription and new protein synthesis regulated by epigenetics play integral roles in the formation of new memories.However,as an important part of epigenetics,the function of chromatin remodeling in learning and memory has been less studied.Here,we showed that SMARCA5(SWI/SNF related,matrix-associated,actin-dependent regulator of chromatin,subfamily A,member 5),a critical chromatin remodeler,was responsible for hippocampus-dependent memory maintenance and neurogenesis.Using proteomics analysis,we found protein expression changes in the hippocampal dentate gyrus(DG)after the knockdown of SMARCA5 during contextual fear conditioning(CFC)memory maintenance in mice.Moreover,SMARCA5 was revealed to participate in CFC memory maintenance via modulating the proteins of metabolic pathways such as nucleoside diphosphate kinase-3(NME3)and aminoacylase 1(ACY1).This work is the first to describe the role of SMARCA5 in memory maintenance and to demonstrate the involvement of metabolic pathways regulated by SMARCA5 in learning and memory.
