This study investigated the expression of lung surfactant proteins SP-B and SP-C, and their modulating factors TTF-1 and PLAGL2 in the fetal lung of rats with fetal growth restriction（FGR）. The rat FGR model was est...This study investigated the expression of lung surfactant proteins SP-B and SP-C, and their modulating factors TTF-1 and PLAGL2 in the fetal lung of rats with fetal growth restriction（FGR）. The rat FGR model was established by prenatal hypoxia in the first stage of pregnancy, 180 rats for experiment served as hypoxia group, and 197 healthy rats served as normal control group. The FGR incidence in hypoxia was compared with that in normal control group. The histological changes in the fetal lung were observed under the light microscope and electronic microscope in two groups. The SP-B, SP-C, TTF-1 and PLAGL2 proteins were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and m RNA in the fetal lung of two groups were detected by using Western blotting and RT-PCR respectively. The FGR rat model was successfully established by using hypoxia. Pathologically the fetal lung developed slowly, and the expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and mR NA in the fetal lung were significantly reduced in hypoxia group as compared with those in normal control group. It was suggested that maternal hypoxia in the first stage of pregnancy could induce FGR, and reduce the expression of SP-B and SP-C, resulting in the disorder of fetal lung development and maturation.展开更多
目的:通过观察芪蛭皱肺颗粒对脂多糖(LPS)致炎大鼠AT-II细胞SP-B表达变化的影响,探讨其在炎症条件下对SP-B的调控作用。方法:原代培养的AT-II细胞,分为空白组、模型组(脂多糖20μg/m L)、中药组(100μg/m L),用药组(均给予脂多糖20μg/m...目的:通过观察芪蛭皱肺颗粒对脂多糖(LPS)致炎大鼠AT-II细胞SP-B表达变化的影响,探讨其在炎症条件下对SP-B的调控作用。方法:原代培养的AT-II细胞,分为空白组、模型组(脂多糖20μg/m L)、中药组(100μg/m L),用药组(均给予脂多糖20μg/m L,芪蛭皱肺颗粒高、中、低剂量分别为1000μg/m L、100μg/m L、10μg/m L);MTT法检测细胞活力;Q-PCR检测各组细胞SP-B m RNA表达水平。结果:与空白组对比,模型组细胞增殖异常,SP-B m RNA表达水平较低(P<0.05),与中药组无明显差别;与造模组比较,用药组细胞增殖减弱,与给药浓度相关,中剂量组SP-B m RNA表达水平显著提高(P<0.01)。结论:芪蛭皱肺颗粒能抑制脂多糖导致的AT-II细胞异常增殖,上调SP-B m RNA的低水平表达。展开更多
探讨加味二陈汤对脾虚痰湿证模型大鼠肺表面活性物质(pulmonary surfactan,PS)相关蛋白A(pulmonary surfactant-associa ted protein A,SP-A)、相关蛋白B(pulmonary surfactant-associa ted protein B,SP-B)表达的影响。采用甘蓝饲喂、...探讨加味二陈汤对脾虚痰湿证模型大鼠肺表面活性物质(pulmonary surfactan,PS)相关蛋白A(pulmonary surfactant-associa ted protein A,SP-A)、相关蛋白B(pulmonary surfactant-associa ted protein B,SP-B)表达的影响。采用甘蓝饲喂、猪脂灌服和香烟烟熏的方法建立脾虚痰湿证大鼠模型,加味二陈汤进行治疗,免疫组化法检测SPA、SP-B表达的变化。结果表明,与健康组比较,模型组大鼠SP-A和SP-B阳性面积明显减少,平均光密度值显著降低,有显著差异(P<0.01);与模型组比较,各治疗组SP-A和SP-B阳性面积显著增加(P<0.01),平均光密度值有不同程度增加,以治疗Ⅱ组大鼠最为明显(P<0.01)。说明加味二陈汤可显著提高模型大鼠SP-A和SP-B表达,对脾虚痰湿证的发生起到改善作用。展开更多
基金supported by the National Natural Science Foundation of China(No.30971072)
文摘This study investigated the expression of lung surfactant proteins SP-B and SP-C, and their modulating factors TTF-1 and PLAGL2 in the fetal lung of rats with fetal growth restriction（FGR）. The rat FGR model was established by prenatal hypoxia in the first stage of pregnancy, 180 rats for experiment served as hypoxia group, and 197 healthy rats served as normal control group. The FGR incidence in hypoxia was compared with that in normal control group. The histological changes in the fetal lung were observed under the light microscope and electronic microscope in two groups. The SP-B, SP-C, TTF-1 and PLAGL2 proteins were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and m RNA in the fetal lung of two groups were detected by using Western blotting and RT-PCR respectively. The FGR rat model was successfully established by using hypoxia. Pathologically the fetal lung developed slowly, and the expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and mR NA in the fetal lung were significantly reduced in hypoxia group as compared with those in normal control group. It was suggested that maternal hypoxia in the first stage of pregnancy could induce FGR, and reduce the expression of SP-B and SP-C, resulting in the disorder of fetal lung development and maturation.
文摘目的:通过观察芪蛭皱肺颗粒对脂多糖(LPS)致炎大鼠AT-II细胞SP-B表达变化的影响,探讨其在炎症条件下对SP-B的调控作用。方法:原代培养的AT-II细胞,分为空白组、模型组(脂多糖20μg/m L)、中药组(100μg/m L),用药组(均给予脂多糖20μg/m L,芪蛭皱肺颗粒高、中、低剂量分别为1000μg/m L、100μg/m L、10μg/m L);MTT法检测细胞活力;Q-PCR检测各组细胞SP-B m RNA表达水平。结果:与空白组对比,模型组细胞增殖异常,SP-B m RNA表达水平较低(P<0.05),与中药组无明显差别;与造模组比较,用药组细胞增殖减弱,与给药浓度相关,中剂量组SP-B m RNA表达水平显著提高(P<0.01)。结论:芪蛭皱肺颗粒能抑制脂多糖导致的AT-II细胞异常增殖,上调SP-B m RNA的低水平表达。
文摘探讨加味二陈汤对脾虚痰湿证模型大鼠肺表面活性物质(pulmonary surfactan,PS)相关蛋白A(pulmonary surfactant-associa ted protein A,SP-A)、相关蛋白B(pulmonary surfactant-associa ted protein B,SP-B)表达的影响。采用甘蓝饲喂、猪脂灌服和香烟烟熏的方法建立脾虚痰湿证大鼠模型,加味二陈汤进行治疗,免疫组化法检测SPA、SP-B表达的变化。结果表明,与健康组比较,模型组大鼠SP-A和SP-B阳性面积明显减少,平均光密度值显著降低,有显著差异(P<0.01);与模型组比较,各治疗组SP-A和SP-B阳性面积显著增加(P<0.01),平均光密度值有不同程度增加,以治疗Ⅱ组大鼠最为明显(P<0.01)。说明加味二陈汤可显著提高模型大鼠SP-A和SP-B表达,对脾虚痰湿证的发生起到改善作用。
