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Engineered SPIONs functionalized with endothelin a receptor antagonist ameliorate liver fibrosis by inhibiting hepatic stellate cell activation
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作者 Marit ten Hove Andreas Smyris +6 位作者 Richell Booijink Lydia Wachsmuth Uwe Hansen Lejla Alic Cornelius Faber Carsten Hӧltke Ruchi Bansal 《Bioactive Materials》 SCIE CSCD 2024年第9期406-426,共21页
Endothelin-1/endothelin A receptor(ET-1/ETAR)pathway plays an important role in the progression of liver fibrosis by activating hepatic stellate cells(HSCs)-a key cell type involved in the pathogenesis of liver fibros... Endothelin-1/endothelin A receptor(ET-1/ETAR)pathway plays an important role in the progression of liver fibrosis by activating hepatic stellate cells(HSCs)-a key cell type involved in the pathogenesis of liver fibrosis.Inactivating HSCs by blocking the ET-1/ETAR pathway using a selective ETAR antagonist(ERA)represents a promising therapeutic approach for liver fibrosis.Unfortunately,small-molecule ERAs possess limited clinical potential due to poor bioavailability,short half-life,and rapid renal clearance.To improve the clinical applicability,we conjugated ERA to superparamagnetic iron-oxide nanoparticles(SPIONs)and investigated the therapeutic efficacy of ERA and ERA-SPIONs in vitro and in vivo and analyzed liver uptake by in vivo and ex vivo magnetic resonance imaging(MRI),HSCs-specific localization,and ET-1/ETAR-pathway antagonism in vivo.In murine and human liver fibrosis/cirrhosis,we observed overexpression of ET-1 and ETAR that correlated with HSC activation,and HSC-specific localization of ETAR.ERA and successfully synthesized ERA-SPIONs demonstrated significant attenuation in TGFβ-induced HSC activation,ECM production,migration,and contractility.In an acute CCl4-induced liver fibrosis mouse model,ERA-SPIONs exhibited higher liver uptake,HSC-specific localization,and ET-1/ETAR pathway antagonism.This resulted in significantly reduced liver-to-body weight ratio,plasma ALT levels,andα-SMA and collagen-I expression,indicating attenuation of liver fibrosis.In conclusion,our study demonstrates that the delivery of ERA using SPIONs enhances the therapeutic efficacy of ERA in vivo.This approach holds promise as a theranostic strategy for the MRI-based diagnosis and treatment of liver fibrosis. 展开更多
关键词 Endothelin A receptor spions Hepatic stellate cells Liver fibrosis
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分子探针Tf-SPION在肝癌早期介入治疗中的应用分析
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作者 彭虹 闵朋 江广斌 《临床和实验医学杂志》 2016年第2期107-110,共4页
目的探讨分子探针转铁蛋白超顺磁性氧化铁颗粒(Tf-SPION)在肝癌早期介入治疗中的应用价值。方法2009年8月至2013年12月临床确诊的肝癌患者120例,均给予早期经导管动脉化疗栓塞介入治疗,在介入治疗前后均进行基于分子探针Tf-SPION的MR诊... 目的探讨分子探针转铁蛋白超顺磁性氧化铁颗粒(Tf-SPION)在肝癌早期介入治疗中的应用价值。方法2009年8月至2013年12月临床确诊的肝癌患者120例,均给予早期经导管动脉化疗栓塞介入治疗,在介入治疗前后均进行基于分子探针Tf-SPION的MR诊断。观察病灶部位的MR影像学特征,特别是对肿瘤富血供组织与肿瘤乏血供组织进行区别判断。在介入治疗后1个月与3个月进行肿瘤治疗效果的判断。结果所有患者均顺利完成MR检查,肝癌病灶部位在T1WI上全部为低信号,T2WI上病灶表现为高信号,周围肝组织的平均表现弥散系数(ADC值)(1.73±0.46)×10^(-3)mm^2/s,肝癌病灶部位的ADC值(1.54±0.40)×10^(-3)mm^2/s。肝癌病灶部位的ADC值明显低于周围肝组织,差异有统计学意义(P<0.05)。肿瘤富血供组织的ADC值明显低于乏血供组织,差异有统计学意义(P<0.05)。介入治疗3个月后的有效率为87.5%,明显高于介入治疗1个月的有效率75.0%,差异有统计学意义(P<0.05)。介入治疗1个月后患者的ADC值为(1.63±0.64)×10^(-3)mm^2/s,介入治疗3个月后的ADC值为(1.78±0.31)×10^(-3)mm^2/s,均明显高于介入治疗前的ADC值(P<0.05)。结论肝癌早期介入治疗能取得比较好的预后效果,分子探针Tf-SPION在介入中的应用能指导治疗方案的选择与疗效的评定,值得推广应用。 展开更多
关键词 肝癌 MR 分子探针Tf-spion 经导管动脉化疗栓塞
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Effects of Sonication Processing on the Behavior of the Synthesis Human Serum Albumin-SPIONs Loaded PLGA Nanoparticles
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作者 S. Vidawati S. Barbosa +1 位作者 P. Taboada V. Mosquera 《Advances in Biological Chemistry》 2019年第6期179-188,共10页
This paper reports the most prominent contributions in the field of biodegradable polymeric nanoparticles from poly (lactic-co-glycolic acid) (PLGA) used as a protein/drug delivery. We use a combination of Human Serum... This paper reports the most prominent contributions in the field of biodegradable polymeric nanoparticles from poly (lactic-co-glycolic acid) (PLGA) used as a protein/drug delivery. We use a combination of Human Serum Albumin (HSA)-superparamagnetic iron oxide nanoparticles (SPIONs) loaded PLGA nanoparticles. To obtain protein stabilization, the optimization of each step of synthesis nanoparticle is required. One of the most common problems in encapsulating protein to PLGA nanoparticles is the presence of several challenges as a problem of instability. We explained how the effect of the various sonication processing on the synthesis HSA-SPIONs loaded PLGA nanoparticles would be one of the crucial parameters for stability. 展开更多
关键词 NANOPARTICLES PLGA spions HSA Sonication
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Spions Increase Biofilm Formation by <i>Pseudomonas aeruginosa</i>
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作者 Carl Haney John J. Rowe Jayne B. Robinson 《Journal of Biomaterials and Nanobiotechnology》 2012年第4期508-518,共11页
Limited research has suggested iron oxide nanoparticles (FeNP) have an inhibitory effect against several different genera of bacteria: Staphylococcus, Bacillus and Pseudomonas spp. In this study we looked at the effec... Limited research has suggested iron oxide nanoparticles (FeNP) have an inhibitory effect against several different genera of bacteria: Staphylococcus, Bacillus and Pseudomonas spp. In this study we looked at the effect of three different sets of Fe3O4 nanoparticles (FeNPs) on the development of Pseudomonas aeruginosa PAO1 biofilms. Two of the tested NPs were SPIONs (Superparamagnetic Iron Oxide Nanoparticles). Exposure of cells to the SPIONs at concentrations up to 200 μg/ml resulted in an increase in biofilm biomass by 16 h under static conditions and a corresponding increase in cell density in the bulk liquid. In contrast, these biofilms had decreased levels of extracellular DNA (eDNA). Fe(II) levels in the supernatants of biofilms formed in the presence of FeNPs exceeded 100 μM compared with 20 μM in control media without cells. Spent cell supernatants had little effect on Fe(II) levels. Cells also had an effect on the aggregation behavior of these nanoparticles. SPIONs incubated with cells exhibited a decrease in the number and size of FeNP aggregates visible using light microscopy. SPIONs resuspended in fresh media or spent culture supernatants formed large aggregates visible in the light microscope upon exposure to a supermagnet;and could be pelleted magnetically in microtitre plate wells. In contrast, SPION FeNPs incubated with cells were unaffected by exposure to the supermagnet and could not be pelleted. The results of this study indicate a need to reconsider the effects of FeNPs on bacterial growth and biofilm formation and the effect the bacterial cells may have on the use and recovery of SPIONs. 展开更多
关键词 spion Iron Oxide Nanoparticles Biofilms PSEUDOMONAS AERUGINOSA MAGNETISM NANOTECHNOLOGY
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Study of Human Serum Albumin-SPIONs Loaded PLGA Nanoparticles for Protein Delivery
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作者 Sri Vidawati Silvia Barbosa +3 位作者 Pablo Taboada Eva Villar Antonio Topete Victor Mosquera 《Advances in Biological Chemistry》 2018年第5期91-100,共10页
The Biodegradable nanoparticles from poly(lactic-co-glycolic acid) (PLGA) have been extensively investigated for sustained and targeted/localized delivery of different agents. Many parameters are required in the synth... The Biodegradable nanoparticles from poly(lactic-co-glycolic acid) (PLGA) have been extensively investigated for sustained and targeted/localized delivery of different agents. Many parameters are required in the synthesis of a biodegradable polymeric nanoparticle. We report the synthesis of human serum albumin (HSA)-superparamagnetic iron oxide nanoparticles (SPIONs) loaded PLGA nanoparticles. All nanoparticles were characterized using a TEM image, UV-Vis spectroscopy measurements, Zeta Potential, and PPMS for magnetizations. This study described and investigated the interesting phenomenon in the synthesis development of HSA-SPIONs loaded PLGA nanoparticles. The result showed that the stability of HSA-SPIONs loaded PLGA nanoparticles for potential applications such as in protein delivery. 展开更多
关键词 NANOPARTICLES PLGA spions HSA
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Imaging and Therapeutic Applications of Optical and Thermal Response of SPION-Based Third Generation Plasmonic Nanodendrimers
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作者 Maryam Tajabadi Mohammad E. Khosroshahi Shahin Bonakdar 《Optics and Photonics Journal》 2015年第7期212-226,共15页
In this study, 9 nm superparamagnetic iron oxide nanoparticles (SPION) were functionalized by polyamidoamine (PAMAM) dendrimer. Using tetracholoroauric acid (HAuCl4), magnetodendrimer (MD) samples were conjugated by g... In this study, 9 nm superparamagnetic iron oxide nanoparticles (SPION) were functionalized by polyamidoamine (PAMAM) dendrimer. Using tetracholoroauric acid (HAuCl4), magnetodendrimer (MD) samples were conjugated by gold nanoparticles (Au-NPs). Two different reducing agents, i.e. sodium borohydride and hydrazine sulfate, and pre-synthesized 10-nm Au-NP were used to evaluate the efficiency of conjugation method. The samples were characterized using X-ray diffractometry (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, UV-visible spectroscopy and fluorescence spectroscopy. The results confirmed that Au- NPs produced by sodium borohydrate and the pre-synthesized 10-nm Au-NPs were capped by MDs whereas the Au-NP prepared by hydrazine sulfate as a reducing agent was entrapped by MDs. Optical properties of the MDs were studied by laser-induced fluorescence spectroscopy (LIF) within a wide range of visible spectrum. Also, based on the thermal analysis, all synthesized nanostructures exhibited a temperature increase using 488 nm and 514 nm wavelengths of a tunable argon laser. The new iron oxide-dendrimer-Au NPs synthesized by sodium borohydrate (IDA- NaBH4) produced the highest temperature increase at 488 nm whereas the other nanostructures particularly pure Au-NPs produced more heating effect at 514 nm. These findings suggest the potential application of these nanocomposites in the field of bioimaging, targeted drug delivery and controlled hyperthermia. 展开更多
关键词 spions PAMAM DENDRIMER Gold Nanoparticles Fluorescence BIOIMAGING Therapy
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Co-delivery of mRNA and SPIONs through amino-ester nanomaterials 被引量:1
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作者 Xiao Luo Weiyu Zhao +6 位作者 Bin Li Xinfu Zhang Chengxiang Zhang Anna Bratas Binbin Deng David W. McComb Yizhou Dong 《Nano Research》 SCIE EI CAS CSCD 2018年第10期5596-5603,共8页
Nanoparticles have been widely explored for combined therapeutic and diagnostic applications. For example, lipid-based nanoparticles have been used to encapsulate multiple types of agents and achieve multi-functions. ... Nanoparticles have been widely explored for combined therapeutic and diagnostic applications. For example, lipid-based nanoparticles have been used to encapsulate multiple types of agents and achieve multi-functions. Herein, we enabled a co-delivery of mRNA molecules and superparamagnetic iron oxide nanoparticles (SPIONs) by using an amino-ester lipid-like nanomaterial. An orthogonal experimental design was used to identify the optimal formulation. The optimal formulation, MPA-Ab-8 LLNs, not only showed high encapsulation of both mRNA and SPIONs, but also increased the r2 relaxivity of SPIONs by more than 1.5-fold in vitro. MPA-Ab-8 LLNs effectively delivered mRNA and SPIONs into cells, and consequently induced high protein expression as well as strong MRI contrast. Consistent herewith, we observed both mRNA-mediated protein expression and an evident negative contrast enhancement of MRI signal in mice. In conclusion, amino-ester nanomaterials demonstrate great potential as delivery vehicles for theranostic applications. 展开更多
关键词 amino-ester nanomaterials lipid-like nanoparticles(LLNs) dual-functional superparamagnetic iron oxide nanoparticles(spions) mRNA delivery magnetic resonance imaging (MRI)
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螯合anti—IL-1βmAb—SPIONs对颞叶癫痫模型的靶向诊治价值
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作者 王艳丽 胡喜斌 +4 位作者 高凌云 吴兴饶 褚旭 周树虎 孔庆霞 《中华医学杂志》 CAS CSCD 北大核心 2018年第33期2678-2682,共5页
目的探讨螯合anti。IL.1BmAb.SPIONs在氯化锂.皮罗卡品诱导的颞叶癫痫模型中的诊治价值。方法将45只颞叶癫痫模型大鼠按照随机数字表法随机平均分为生理盐水组、plain—SPIONs组、anti—IL-1βmAb—SPIONs组,各组于癫痫发作后3d和14... 目的探讨螯合anti。IL.1BmAb.SPIONs在氯化锂.皮罗卡品诱导的颞叶癫痫模型中的诊治价值。方法将45只颞叶癫痫模型大鼠按照随机数字表法随机平均分为生理盐水组、plain—SPIONs组、anti—IL-1βmAb—SPIONs组,各组于癫痫发作后3d和14d分别进行注射等量粒子,粒子注射前及注射后4h分别行MRI扫描并测量T,值。注射粒子14d后的SD大鼠,观察铁粒子在癫痫病灶的分布情况,观察各组神经元缺失、星形胶质细胞增生和小胶质细胞活化情况,并检测IL-1β、NF—KBp65在各组中的表达情况。结果癫痫大发作14d后,注射anti-IL-1βmAb.SPIONs后T:值84±14,明显低于生理盐水组,神经元缺失、星形胶质细胞增生、小胶质细胞活化现象明显改善,IL-1β、NF—KBp65表达也明显降低。结论anti—IL-18mAb—SPIONs可以透过血脑屏障对颞叶癫痫病灶进行靶向定位和治疗。 展开更多
关键词 颞叶癫痫 IL-1β单克隆抗体 超顺磁性氧化铁纳米粒子 靶向定位 靶向治疗
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PEG/PEI/PASP修饰的磁性氧化铁纳米粒子的制备及磁共振成像的研究 被引量:2
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作者 钟良 谢松伯 +4 位作者 夏天 杨凤丹 聂婉 梁广钦 张宝林 《人工晶体学报》 EI CAS 北大核心 2019年第8期1549-1556,共8页
以聚乙二醇(Polyethylene glycol,PEG)为溶剂及反应剂,聚乙烯亚胺(Polyethyleneimine,PEI)为添加剂,乙酰丙酮铁(Fe(acac) 3 )为铁源,采用高温热分解法制备了超顺磁性氧化铁纳米粒子(Super-paramagnetic iron oxide nanoparticles,SPIONs... 以聚乙二醇(Polyethylene glycol,PEG)为溶剂及反应剂,聚乙烯亚胺(Polyethyleneimine,PEI)为添加剂,乙酰丙酮铁(Fe(acac) 3 )为铁源,采用高温热分解法制备了超顺磁性氧化铁纳米粒子(Super-paramagnetic iron oxide nanoparticles,SPIONs)。通过物理法将聚天冬氨酸(Poly aspartic acid,PASP)成功修饰在PEG、PEI修饰的超顺磁性氧化铁纳米粒子(PEG/PEI-SPIONs)表面,得到PEG/PEI/PASP-SPIONs。透射电子显微镜表征结果显示,PEG/PEI/PASP-SPIONs具有良好的分散性,且平均粒径为(10.8±2.0) nm(平均值±标准差)。小鼠脑部磁共振成像实验表明,PEG/PEI/PASP-SPIONs可以长时间在小鼠脑部呈现磁共振成像(MRI)效果,PEG/PEI/PASP-SPIONs在小鼠脑部的成像时间比PEG/PEI-SPIONs和PEG/PEI/Tween 80-SPIONs的成像时间更短,可用于磁共振成像的造影剂。 展开更多
关键词 spions超顺磁性氧化铁纳米粒子 聚天冬氨酸 MRI 造影剂
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物理和化学法合成钙离子敏感MRI造影剂对比研究
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作者 徐鹏飞 沈智威 +1 位作者 张宝林 吴仁华 《人工晶体学报》 EI CAS CSCD 北大核心 2016年第5期1335-1341,共7页
以聚乙烯亚胺(PEI)为添加剂,在聚乙二醇(PEG)中高温热解乙酰丙酮铁(Fe(acac)3)合成超顺磁性氧化铁纳米粒子(PEI/PEG-SPIONs),再与钙离子螯合剂EGTA分别通过物理混合法和EDC/sulfo-NHS化学法合成了对钙离子敏感的磁共振成像(MRI)造影剂EG... 以聚乙烯亚胺(PEI)为添加剂,在聚乙二醇(PEG)中高温热解乙酰丙酮铁(Fe(acac)3)合成超顺磁性氧化铁纳米粒子(PEI/PEG-SPIONs),再与钙离子螯合剂EGTA分别通过物理混合法和EDC/sulfo-NHS化学法合成了对钙离子敏感的磁共振成像(MRI)造影剂EGTA-SPIONs/P(物理混合)和EGTA-SPIONs/C(化学法)。PEI/PEGSPIONs、EGTA-SPIONs/P和EGTA-SPIONs/C分散于HEPES缓冲液中的电泳粒度分别为27.6±3.2 nm、32.1±5.2 nm和36.1±4.8 nm;加入Ca2+后EGTA-SPIONs/P和EGTA-SPIONs/C的平均电泳粒度均趋于变大,呈双峰分布,再加入EDTA后,平均电泳粒度又都趋于减小。MRI测试结果表明,当Ca2+浓度从0.8 mmol/L增加到1.2mmol/L时,EGTA-SPIONs/P和EGTA-SPIONs/C样品的T2值分别增加了33%和33.5%;将0.8 mmol/L EDTA加入混合0.8 mmol/L Ca2+的EGTA-SPIONs/P和EGTA-SPIONs/C的分散液中,其T2值分别降低了14%和42%。EGTA-SPIONs/P及EGTA-SPIONs/C对Ca2+浓度的增加均有反映,但Ca2+@EGTA-SPIONs/P的EDTA逆转效果不如Ca2+@EGTA-SPIONs/C的明显,化学法合成的EGTA-SPIONs/C更适合作为钙离子敏感剂。 展开更多
关键词 spions EGTA 钙离子敏感剂 MRI T2造影剂 EDTA逆转
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Measurements of Pulsed 532 nm Laser Breakdown Spectroscopy of Synthesized Magnetite Nanoferrofluid
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作者 Mohammad E. Khosroshahi Maryam Tajabadi 《World Journal of Nano Science and Engineering》 2018年第3期39-55,共17页
We describe the results of 532 nm pulse laser-induced breakdown spectroscopy (LIBS) of two samples of magnetite nanoparticles (SPIONs) nanoferrofluid synthesized at room (S1) and elevated temperatures (S2) and at thre... We describe the results of 532 nm pulse laser-induced breakdown spectroscopy (LIBS) of two samples of magnetite nanoparticles (SPIONs) nanoferrofluid synthesized at room (S1) and elevated temperatures (S2) and at three different laser energy levels and pulse frequency. The size of magnetite nanoparticles, size distribution, magnetic crystalline phase and magnetization were analyzed and measured using transmission electron microscopy (TEM), X-ray diffraction spectroscopy (XRD) and vibrating sample magnetometry (VSM). The SPIONs showed a distribution between 4 - 22 nm with a peak about 12 nm and saturation magnetization of about 65 emu/g. The Saha-Boltzmann analysis of spectra for medium energy level (1050 mJ) yields plasma temperatures of (3881 ± 200) K and (26,047 ± 200) K for Fe I and OV as the lowest and highest temperatures respectively. A range of corresponding electron density (Ne-) of (0.47 - 6.80) × 1020, (0.58 - 8.30) × 1020 and (0.69 - 9.96) × 1020 cm-3?were determined at 860, 1050 and 1260 mJ respectively using the estimated CCD pictures. The results confirmed a higher elements ratio for S1 than S2 and the signal intensity indicated a non-linear behaviour as a function of pulse frequency with the maximum ratio value at 3 Hz. At higher frequency of 6 Hz no such turning point was observed. The highest and lowest temperatures corresponded to Fe I and OV respectively. The LIBS technique can be utilized to study, characterize and determine the elements ratio required in most applications involving the synthesizing process. 展开更多
关键词 LASER-INDUCED BREAKDOWN Spectroscopy Second HARMONIC Generation spion Nanoferrofluid Elements Ratio
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不同粒径超顺磁性氧化铁纳米粒子的合成及其在交变磁场中的磁热效应 被引量:3
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作者 韩栋 张宝林 +2 位作者 苏礼超 韩贵华 汪晟 《材料工程》 EI CAS CSCD 北大核心 2019年第4期84-90,共7页
采用多醇热解法制备3种不同粒径的超顺磁性氧化铁纳米粒子(SPIONs),合成的SPIONs含Fe_3O_4晶相,分散性好,平均粒径分别为8.7,12.6nm和15.3nm,且在300K下,3种SPIONs均呈超顺磁性。将不同粒径、不同浓度的SPIONs水分散液置于频率为425kHz... 采用多醇热解法制备3种不同粒径的超顺磁性氧化铁纳米粒子(SPIONs),合成的SPIONs含Fe_3O_4晶相,分散性好,平均粒径分别为8.7,12.6nm和15.3nm,且在300K下,3种SPIONs均呈超顺磁性。将不同粒径、不同浓度的SPIONs水分散液置于频率为425kHz、磁场强度为5.3kA·m^(-1)的交变磁场(ACMF)中进行升温实验。探讨比能量吸收率值与SPIONs粒径之间的关系,计算布朗弛豫时间及尼尔弛豫时间。结果表明:SPIONs水分散液的升温速率随SPIONs的粒径增大而增大,初始温度为20℃时,粒径为8.7,12.6nm和15.3nm的SPIONs水分散液(2mg·mL^(-1))在480s内温度分别升高了25,27,35℃。尼尔弛豫时间比布朗弛豫时间小,说明磁热效应主要来自于尼尔弛豫损耗。SPIONs粒径越大,比能量吸收率SAR值越高,最高可达810W·g^(-1),且SAR值与SPIONs水分散液的浓度呈负相关关系。 展开更多
关键词 超顺磁性氧化铁纳米粒子 粒径 交变磁场 比能量吸收率 磁热效应
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二肉豆蔻酰磷脂酰胆碱修饰的氧化铁纳米粒子在PC-12细胞内的分布 被引量:1
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作者 韩贵华 张宝林 +3 位作者 苏礼超 黄银平 范子梁 赵应征 《材料导报》 EI CAS CSCD 北大核心 2019年第6期1047-1051,共5页
有机物包覆的超顺磁性氧化铁纳米粒子(Superparamagnetic iron oxide nanoparticles,SPIONs)因具有良好的水溶性和生物相容性而被越来越多地用作生物医学研究的工具。采用简便的高温热分解法合成聚乙二醇(Polyethylene glycol,PEG)和聚... 有机物包覆的超顺磁性氧化铁纳米粒子(Superparamagnetic iron oxide nanoparticles,SPIONs)因具有良好的水溶性和生物相容性而被越来越多地用作生物医学研究的工具。采用简便的高温热分解法合成聚乙二醇(Polyethylene glycol,PEG)和聚乙烯亚胺(Polyethyleneimine,PEI)修饰的超顺磁性氧化铁纳米粒子(PEG/PEI-SPIONs),再在其表面通过氢键相互作用接枝二肉豆蔻酰磷脂酰胆碱(1, 2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC),成功制备了DMPC-SPIONs。由热重结果分析可知DMPC的接枝率约为31.7%(质量分数)。将PEG/PEI-SPIONs和DMPC-SPIONs分别与PC-12细胞孵化,对孵化后的细胞进行表征和分析发现,大量的DMPC-SPIONs进入了细胞,而PEG/PEI-SPIONs进入细胞内的量较少。这表明DMPC对于氧化铁纳米粒子进入细胞起到关键作用。通过透射电镜观察与DMPC-SPIONs孵化的PC-12细胞发现,氧化铁纳米粒子密集分布于溶酶体、线粒体、内质网和细胞核膜表面,另外,也有一部分DMPC-SPIONs分布在细胞膜上的纤毛附近,并观察到细胞膜对纳米粒子的内吞现象。DMPC-SPIONs良好的膜透过性及在胞内细胞器的密集分布使其在磁热疗、磁共振成像、药物输运等生物医学领域具有广阔的应用前景。 展开更多
关键词 超顺磁性氧化铁纳米粒子 修饰 二肉豆蔻酰磷脂酰胆碱 细胞内分布 透射电镜形貌
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磁性细菌纤维素复合材料的原位合成及其表征 被引量:1
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作者 韦峰 张蕾 +1 位作者 陈辰 孙东平 《纤维素科学与技术》 CAS CSCD 2020年第3期19-25,69,共8页
合成了一种超顺磁性氧化铁纳米粒子(Superparamagnetic Iron Oxide Nanoparticles,SPIONs),并通过原位培养合成方法制得磁性细菌纤维素(Magnetic Bacterial Cellulose,MBC)。通过X-射线衍射(XRD)、透射电子显微镜(TEM)、傅里叶红外光谱(... 合成了一种超顺磁性氧化铁纳米粒子(Superparamagnetic Iron Oxide Nanoparticles,SPIONs),并通过原位培养合成方法制得磁性细菌纤维素(Magnetic Bacterial Cellulose,MBC)。通过X-射线衍射(XRD)、透射电子显微镜(TEM)、傅里叶红外光谱(FT-IR)和磁滞回线(VSM)等方法测定了SPIONs的性质。结果显示其粒子大小约为20 nm,分散性良好,饱和磁强度为62 emu/g。对BC和MBC进行了扫描电子显微镜(SEM)、热重(TGA)和拉力测试。结果显示,纳米粒子均匀的分散在纤维表面,负载率达到5%。拉力测试显示BC和MBC有着良好的机械性能,MBC比BC有更高的杨氏模量和拉伸强度。由细胞实验可得,在不同天数下,MBC上的细胞数量以及细胞粘附面积比BC都要有所增加。MBC和BC的生物毒性很低,都有着很好地生物相容性。 展开更多
关键词 超顺磁性性氧化铁纳米粒子 细菌纤维素 原位合成 细胞增殖与粘附
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泊松(Poisson)方程特解的待定函数解法 被引量:1
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作者 夏志 《辽宁工学院学报》 2006年第1期66-69,共4页
针对物理学和工程技术理论中,有许多典型问题都可归结为泊松(Poisson)方程的边值问题,介绍了当泊松方程的右端函数f呈现某些特殊形式时,可用待定函数的方法寻求它的特解,从而能简便的求解泊松方程的边值问题。
关键词 泊松方程 特解 待定函数法 分离变量法
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卒中干细胞治疗中纳米造影剂活体示踪研究进展
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作者 陈超 张春富 +1 位作者 王永亭 杨国源 《中国卒中杂志》 2012年第12期966-972,共7页
干细胞移植可能是将来治疗卒中的理想方法之一。无创示踪干细胞在脑中的存活、增殖分化、迁移,以及与宿主的整合是确定干细胞移植治疗的有效性及安全性的关键。近年来,随着分子生物学技术及材料科学的发展,愈来愈多的无创模式和与之相... 干细胞移植可能是将来治疗卒中的理想方法之一。无创示踪干细胞在脑中的存活、增殖分化、迁移,以及与宿主的整合是确定干细胞移植治疗的有效性及安全性的关键。近年来,随着分子生物学技术及材料科学的发展,愈来愈多的无创模式和与之相应的新型造影剂被用于移植细胞的活体示踪,并已在动物模型取得了初步成效。本文对应用纳米造影剂在卒中干细胞治疗中活体示踪的研究进展进行综述。 展开更多
关键词 干细胞 卒中 超顺磁氧化铁 量子点 多模态造影
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Self-assembled multifunctional nanotheranostics against circulating tumor clusters in metastatic breast cancer
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作者 Ramya Dhandapani Swaminathan Sethuraman +1 位作者 Uma Maheswari Krishnan Anuradha Subramanian 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2023年第4期1711-1725,共15页
Circulating tumor clusters(CTC)disseminating from the primary tumor are responsible for secondary tumor formation where the conventional treatments such as chemotherapy and radiotherapy does not prevent the metastasis... Circulating tumor clusters(CTC)disseminating from the primary tumor are responsible for secondary tumor formation where the conventional treatments such as chemotherapy and radiotherapy does not prevent the metastasis at locally advanced stage of breast cancer.In this study,a smart nanotheranostic system has been developed to track and eliminate the CTCs before it can colonize at a new site,which would reduce metastatic progression and increase the five-year survival rate of the breast cancer patients.Targeted multiresponsive(magnetic hyperthermia and pH)nanomicelles incorporated with NIR fluorescent superparamagnetic iron oxide nanoparticles were developed based on self-assembly for dual modal imaging and dual toxicity for spontaneous killing of CTCs in blood stream.A heterogenous tumor clusters model was developed to mimic the CTCs isolated from breast cancer patients.The nanotheranostic system was further evaluated for the targeting property,drug release kinetics,hyperthermia and cytotoxicity against developed CTC model in vitro.In vivo model in BALB/c mice equivalent to stageⅢandⅣhuman metastatic breast cancer was developed to evaluate the biodistribution and therapeutic efficacy of micellar nanotheranostic system.Reduced CTCs in blood stream and low distant organ metastasis after treatment with the nanotheranostic system demonstrates its potential to capture and kill the CTCs that minimize the secondary tumor formation at distant sites. 展开更多
关键词 Self-assembly Hybrid nanotheranostic system Circulating tumor clusters Advanced breast cancer Cancer stem cells Heterogenous clusters spions NIR Metastasis CD44
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超顺磁性氧化铁纳米粒作为磁共振成像造影剂的研究概况 被引量:4
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作者 饶跃峰 李慧婷 +1 位作者 曹国洪 卢晓阳 《中国药学杂志》 CAS CSCD 北大核心 2014年第15期1291-1294,共4页
目的综述超顺磁性氧化铁纳米粒(superparamagnetic iron oxide nanoparticles,SPION)作为磁共振成像造影剂的国内外研究进展。方法在Pubmed和Google资源上检索近30年来国内外相关资料,阐述磁共振成像造影剂原理和分类,介绍超顺磁性氧化... 目的综述超顺磁性氧化铁纳米粒(superparamagnetic iron oxide nanoparticles,SPION)作为磁共振成像造影剂的国内外研究进展。方法在Pubmed和Google资源上检索近30年来国内外相关资料,阐述磁共振成像造影剂原理和分类,介绍超顺磁性氧化铁纳米粒的结构、特性及制备方法,对新一代超顺磁性氧化铁纳米粒造影剂在磁共振成像技术及其在生物医学中应用进行总结和评论。结果超顺磁性氧化铁纳米粒造影剂已成为磁共振成像中重要的临床诊断药物,在肝脏、淋巴系统和胰腺、中枢神经系统及肾脏疾病的诊断和治疗方面已取得系列成果。结论超顺磁性氧化铁纳米粒在医学基础研究和临床应用上具有广阔的应用前景,开发具有靶向性、高效、安全的超顺磁性氧化铁纳米粒造影是目前该领域研究主要方向之一。 展开更多
关键词 超顺磁氧化铁性纳米粒 造影剂 磁共振成像 靶向性
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Influence of rare earth ion substitutions on the structural,optical,transport,dielectric,and magnetic properties of superparamagnetic iron oxide nanoparticles 被引量:3
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作者 R.M.KERSHI F.M.ALI M.A.SAYED 《Journal of Advanced Ceramics》 SCIE CSCD 2018年第3期218-228,共11页
Superparamgnetic Fe_3O_4 and RE:Fe_3O_4(RE=Dy,Nd,La)nanoparticles with an average crystallite size in the range of 15–24 nm,were synthesized by co-precipitation method.The samples were characterized using X-ray diffr... Superparamgnetic Fe_3O_4 and RE:Fe_3O_4(RE=Dy,Nd,La)nanoparticles with an average crystallite size in the range of 15–24 nm,were synthesized by co-precipitation method.The samples were characterized using X-ray diffraction(XRD),scanning electron microscopy(SEM),vibrating sample magnetometer(VSM),UV–Vis spectroscopy,LCR bridge,and two-probe technique.X-ray diffraction patterns of all the investigated samples reveal the typical phase of magnetite structure,with a small contribution of orthoferrite(NdFeO_3)as a secondary phase in Nd:Fe_3O_4 sample.The saturation magnetization(M_s)of the samples has values in the range from 41.8 to 52.3 emu/g,and decreases with RE ion doping depending on the ionic radius.Negligible values of the coercivity H_c and remanenceM_r,indicate the superparamagnetic nature of the investigated samples.The calculated values of indirect optical band gap of Fe_3O_4 and RE:Fe_3O_4 nanoparticles are in the range of0.9–1.25 eV.The dielectric constant of the samples decreases,while their activation energy increases with the increasing of ionic radii of dopants. 展开更多
关键词 superparamgnetic iron oxide nanoparticles (spions) RARE earth (RE) ions structure optical and activation ENERGIES electrical CONDUCTIVITY DIELECTRIC constant saturationmagnetization
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Magnetic nanoparticles for environmental and biomedical applications: A review 被引量:19
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作者 Leena Mohammed Hassan G.Gomaa +1 位作者 Doaa Ragab Jesse Zhu 《Particuology》 SCIE EI CAS CSCD 2017年第1期1-14,共14页
Engineered magnetic nanoparticles (MNPs) hold great potential in environmental, biomedical, and clin- ical applications owing to their many unique properties. This contribution provides an overview of iron oxide MNP... Engineered magnetic nanoparticles (MNPs) hold great potential in environmental, biomedical, and clin- ical applications owing to their many unique properties. This contribution provides an overview of iron oxide MNPs used in environmental, biomedical, and clinical fields. The first part discusses the use of MNPs for environmental purposes, such as contaminant removal, remediation, and water treatment, with a focus on the use of zero-valent iron, magnetite (Fe304), and maghemite (~,-Fe203) nanoparticles, either alone or incorporated onto membrane materials. The second part of this review elaborates on the use of MNPs in the biomedical and clinical fields with particular attention to the application of superparamag- netic iron oxide nanoparticles (SP1ONs), which have gained research focus recently owing to their many desirable features such as biocompatibility, biodegradability, ease of synthesis and absence of hysteresis. The properties of MNPs and their ability to work at both cellular and molecular levels have allowed their application in vitro and in vivo including drug delivery, hyperthermia treatment, radio-therapeutics, gene delivery, and biotherapeutics. Physiochemical properties such as size, shape, and surface and magnetic properties as well as agglomeration of MNPs and methods to enhance their stability are also discussed. 展开更多
关键词 Magnetic nanoparticle Water treatment spion Biomedical application
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