SR proteins are essential splicing factors required for constitutive splicing and serving as key regulators of alternative RNA splicing. SR proteins purified from late vaccinia virus infected cells (SR-VV) are hypo-ph...SR proteins are essential splicing factors required for constitutive splicing and serving as key regulators of alternative RNA splicing. SR proteins purified from late vaccinia virus infected cells (SR-VV) are hypo-phosphorylated and functionally inactivated as splicing regulatory proteins by a virus-induced partial dephosphorylation, similar to that seen late during an adenovirus infection [Kanopka et al., Nature 393, 185-187, 1998; Huang et al., submitted). In addition, incubating SR-VV or SR-Ad under conditions which restores the phospho-epitopes to the SR proteins regains their activity as splicing represser proteins. Here, we showed that that several genes were up-regulated in at least 50% chemoresistant AML patients, of展开更多
文摘SR proteins are essential splicing factors required for constitutive splicing and serving as key regulators of alternative RNA splicing. SR proteins purified from late vaccinia virus infected cells (SR-VV) are hypo-phosphorylated and functionally inactivated as splicing regulatory proteins by a virus-induced partial dephosphorylation, similar to that seen late during an adenovirus infection [Kanopka et al., Nature 393, 185-187, 1998; Huang et al., submitted). In addition, incubating SR-VV or SR-Ad under conditions which restores the phospho-epitopes to the SR proteins regains their activity as splicing represser proteins. Here, we showed that that several genes were up-regulated in at least 50% chemoresistant AML patients, of
