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引起梨花枯和芽枯的Pseudomonas syringae pv.syringae病原细菌鉴定 被引量:8
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作者 邱文 徐福寿 +5 位作者 谢关林 徐丽慧 怀雁 李斌 余山红 钱军 《中国农业科学》 CAS CSCD 北大核心 2008年第9期2657-2662,共6页
【目的】明确在中国发生的梨花枯和芽枯的确切病原菌。【方法】用普通细菌学方法、电镜观察、Koch氏病原假说测定、Biolog、脂肪酸分析、PCR及与标准对照菌株的比较。【结果】从16个病样中分离获得12菌株,6株代表菌株显示出与Pseudomona... 【目的】明确在中国发生的梨花枯和芽枯的确切病原菌。【方法】用普通细菌学方法、电镜观察、Koch氏病原假说测定、Biolog、脂肪酸分析、PCR及与标准对照菌株的比较。【结果】从16个病样中分离获得12菌株,6株代表菌株显示出与Pseudomonas syringaepv.syringae3株标准对照菌株相似的致病反应,它们的Biolog和脂肪酸分析的相似度分别为0.57~0.86和0.58~0.81,PCR和序列测定结合上述结果证实了P.syringaepv.syringae为该病的病原菌。【结论】首次证实了中国梨树上的花枯和芽枯可由P.syringaepv.syringae引起。 展开更多
关键词 梨花枯 梨芽枯 PSEUDOMONAS syringae pv.syringae 证实
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植物病原细菌Pseudomonas syringae pv.tomato基因组中的信号肽分析 被引量:12
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作者 刘雅婷 李正跃 +2 位作者 朱有勇 李成云 李永忠 《遗传》 CAS CSCD 北大核心 2005年第6期959-964,共6页
应用SignaIP 3.0对植物病原细菌Pseudomonas syringae pv.tomato DC3000菌株基因组中的细菌染色体全部5 615个ORFs进行了分析,确定其中679个ORFs所编码蛋白质的N-端有信号肽序列,其中已经命名并有注释的有107个ORFs.信号肽的长度以19~3... 应用SignaIP 3.0对植物病原细菌Pseudomonas syringae pv.tomato DC3000菌株基因组中的细菌染色体全部5 615个ORFs进行了分析,确定其中679个ORFs所编码蛋白质的N-端有信号肽序列,其中已经命名并有注释的有107个ORFs.信号肽的长度以19~31个氨基酸居多,其中最多的是23个氨基酸的信号肽.具有信号肽的ORFs编码蛋白的长度大多为101~400个氨基酸之间.同时,对组成信号肽的氨基酸种类作了系统的分析,发现组成信号肽的氨基酸中非极性氨基酸占48.54%,极性氨基酸占18.67%,带负电荷氨基酸占24.54%,带正电荷氨基酸仅占8.00%,出现最多的3种氨基酸依次为亮氨酸、丙氨酸和丝氨酸,最少的氨基酸是异亮氨酸,在切割位点-1端的氨基酸中83.21%均为丙氨酸,在切割位点后3位的氨基酸中最多的氨基酸也是丙氨酸.通过分析确定628个分泌类信号肽,36个信号肽具有RR-motif的保守区段,15个脂蛋白类信号肽,未发现Prepiln-like信号肽和Bacteriocin and Pheromone信号肽. 展开更多
关键词 PSEUDOMONAS syringae pv.tomato ORF 信号肽 RR-motif 脂蛋白
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烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1信号肽预测及分析 被引量:5
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作者 王铁霖 李晶 +1 位作者 杨玉文 赵廷昌 《中国烟草学报》 EI CAS CSCD 北大核心 2016年第1期92-100,共9页
利用Signal P 4.0、Lipo P 1.0及TMHMM v2.0对烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1菌株基因组中信号肽的数量、长度和氨基酸组成进行了预测及分类。结果确定其中432个ORFs(Open reading frame)所编码的N端有信号肽序... 利用Signal P 4.0、Lipo P 1.0及TMHMM v2.0对烟草野火病菌Pseudomonas syringae pv.tabaci yuexi-1菌株基因组中信号肽的数量、长度和氨基酸组成进行了预测及分类。结果确定其中432个ORFs(Open reading frame)所编码的N端有信号肽序列,占全部ORFs的8.81%。其中351条分泌型信号肽(SPI),81条脂蛋白型信号肽(SPII)。在分泌型信号肽中,信号肽的长度为11~42个氨基酸,以长度为22个氨基酸的信号肽最多。同源性分析结果显示,具有相同信号肽序列的不同蛋白序列之间是高度保守的。该研究提供了野火病原菌致病因子的备选基因,提高该病菌致病因子的筛选效率。 展开更多
关键词 Pseudomonas syringae pv.tabaci 信号肽 Signal P 4.0 Lipo P 1.0 TMHMM v2.0
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hrpZ_(Psg12) Gene of Pseudomonas syringae pv.glycinea can Enhance Pathogenicity of the Pathogen on Soybean and Cause the Hypersensitive Response of Tobacco
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作者 张佳环 李娟 高洁 《Plant Diseases and Pests》 CAS 2011年第3期9-13,共5页
[ Objective ] The paper was to confirrm the effect of hrpZpsg12 gene on the pathogenicity of Pseudomonas syringae pv. glycinea. [ Method ] hrpZpsg12 gene was cloned from P. syringae using PCR method. The knockout plas... [ Objective ] The paper was to confirrm the effect of hrpZpsg12 gene on the pathogenicity of Pseudomonas syringae pv. glycinea. [ Method ] hrpZpsg12 gene was cloned from P. syringae using PCR method. The knockout plasmid pKNOCK-Cm with suicide characteristics and cosmid pUFR034 with complementation func- tion were used to construct the mutation vector pKNOCK477-7 and complementary vector pUFR1026-68 of hrpZpsg12 gene, the mutant 477-1 and the functional com- plementation unit 1026-5 of the gene was also screened out. Three strains including wild-type Psg12, mutant 477-1 and complementary unit 1026-5 were simultane- ously inoculated into soybean leaves and tobacco leaves, then pathogenicity determination and hypersensitive reaction analysis were carried out. [ Result] All the inoculated leaves of soybean and tobacco produced reaction lesion. However, the sizes of reaction lesion were different. The lesion in the leaves inoculated with Psgl2 was relatively large, while the lesion in the leaves inoculated with 477-1 was relatively small; the lesion of complementary unit 1026-5 was similar to wild- type Psgl2. Analysis of reproduction quantity of bacteria in lesions showed that the reproduction quantity of wild-type Psg12 was the highest, while that of mutant 477-1 was the lowest. The reproduction quantity of complementary unit 1026-5 was similar to that of wild-type Psg12. [ Conclusion] hrpZpsg12 gene could enhance the pathogenicity of P. syrimgae on Soybean and produce hypersensitive response in tobacco. 展开更多
关键词 Pseudomonas syringae pv. glycinea hrpZpsg2 gene Mutant PATHOGENICITY China
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不同抗性烟草品种感染Pseudomonas syringae pv.tabaci病菌后几种酶活性测定 被引量:12
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作者 汤会君 张俊华 魏向峰 《东北农业大学学报》 CAS CSCD 2005年第4期430-434,共5页
过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性均与烟草品种抗野火病呈正相关。抗感品种健康植株的多酚氧化酶、过氧化物酶活性差异不显著,苯丙氨酸解氨酶活性差异显著。抗感品种接种后POD活性在12,16d出现两次高峰,PPO... 过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性均与烟草品种抗野火病呈正相关。抗感品种健康植株的多酚氧化酶、过氧化物酶活性差异不显著,苯丙氨酸解氨酶活性差异显著。抗感品种接种后POD活性在12,16d出现两次高峰,PPO活性在10,14d出现两次高峰,PAL活性在10,16d出现两次高峰。 展开更多
关键词 烟草野火病 抗感品种 PSEUDOMONAS syringae PPO POD PAL
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Pear Blossom Blast Caused by Pseudomonas syringae pv. syringae in China 被引量:1
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作者 QIU Wen HUAI Yan +5 位作者 XU Fu-shou XU Li-hui XIE Guan-lin LI Bin YU Shan-hong LIU Jun-ying 《Agricultural Sciences in China》 CAS CSCD 2008年第9期1091-1096,共6页
This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch's postulate test, Biolog, fatty acid... This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch's postulate test, Biolog, fatty acid methyl esters (FAMEs), and a polymerase chain reaction (PCR) test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae. 展开更多
关键词 pear blossom blast pear bud blast Pseudomonas syringae pv. syringae confirmation
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Functional identification of phenazine biosynthesis genes in plant pathogenic bacteria Pseudomonas syringae pv. tomato and Xanthomonas oryzae pv. oryzae 被引量:1
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作者 LI Wen XU You-ping +4 位作者 Jean-Pierre Munyampundu XU Xin QI Xian-fei GU Yuan CAI Xin-zhong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第4期812-821,共10页
Phenazines are secondary metabolites with broad spectrum antibiotic activity and thus show high potential in biological control of pathogens. In this study, we identified phenazine biosynthesis (phz) genes in two ge... Phenazines are secondary metabolites with broad spectrum antibiotic activity and thus show high potential in biological control of pathogens. In this study, we identified phenazine biosynthesis (phz) genes in two genome-completed plant pathogenic bacteria Pseudomonas syringae pv. tomato (Pst) DC3000 and Xanthomonas oryzae pv. oryzae (Xoo) PXO99A. Unlike the phz genes in typical phenazine-producing pseudomonads, phz homologs in Pst DC3000 and Xoo PXO99A consisted of phzC/D/E/F/G and phzC/E1/E2/F/G, respectively, and the both were not organized into an operon. Detection experiments demonstrated that phenazine-l-carboxylic acid (PCA) of Pst DC3000 accumulated to 13.4 IJg L-1, while that of Xoo PXO99A was almost undetectable. Moreover, Pst DC3000 was resistant to 1 mg mL-1 PCA, while Xoo PXO99A was sensitive to 50 IJg mL ~ PCA. Furthermore, mutation of phzF blocked the PCA production and significantly reduced the pathogenicity of Pst DC3000 in tomato, while the complementary strains restored these phenotypes. These results revealed that Pst DC3000 produces low level of and is resistant to phenazines and thus is unable to be biologically controlled by phenazines. Additionally, phz-mediated PCA production is required for full pathogenicity of Pst DC3000. To our knowledge, this is the first report of PCA production and its function in pathogenicity of a plant pathogenic P. syringae strain. 展开更多
关键词 PATHOGENICITY phenazine biosynthesis genes phenazine-l-carboxylic acid plant pathogenic bacteria Pseudomonas syringae pv. tomato Xanthomonas oryzae pv. oryzae
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不同抗性烟草品种感染Pseudomonas syringae pv.tabaci病菌后几种酶活性测定 被引量:12
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作者 魏相峰 汤会君 《检验检疫科学》 2006年第2期17-19,共3页
〔目的〕揭示烟草品种抗野火病机制,推动和指导烟草抗病品种选育与抗源的合理布局。〔方法〕给不同抗性的烟草品种分别接种烟草野火病菌后测定其几种酶的活性。〔结果〕过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性均与... 〔目的〕揭示烟草品种抗野火病机制,推动和指导烟草抗病品种选育与抗源的合理布局。〔方法〕给不同抗性的烟草品种分别接种烟草野火病菌后测定其几种酶的活性。〔结果〕过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性均与烟草品种抗野火病呈正相关。抗、感品种健康植株的多酚氧化酶、过氧化物酶的活性差异不显著,苯丙氨酸解氨酶活性差异显著。抗感品种接种后POD的活性变化分别在12天、16天出现2次高峰,PPO的活性变化分别在10天、14天出现2次高峰,PAL的活性变化分别在10天、16天出现2次高峰。〔结论〕3种酶活性变化出现的峰值强弱可作为早期鉴定烟草品种抗野火病的一种有价值的生理指标。 展开更多
关键词 烟草野火病 抗感品种 PSEUDOMONAS syringae PPO POD PAL
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Hepatoprotective Effects of Folium syringae Extracts Against Ethanolinduced Acute Liver Injury
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作者 He Jing-shan Lin Yue-xia +7 位作者 Li Chang-wen Li Rui Chang Yi-cong Li Ying Shi Chen-xi Ma Xin Li Zhi Liu Fang-ping 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第4期62-70,共9页
The aim of the study was to investigate the hepatoprotective effects of Folium syringae(FS) extracts against ethanolinduced acute liver injury. Mice and primary hepatocytes were pretreated with FS extracts at differen... The aim of the study was to investigate the hepatoprotective effects of Folium syringae(FS) extracts against ethanolinduced acute liver injury. Mice and primary hepatocytes were pretreated with FS extracts at different dosages before ethanol administration. Transaminases, glutathione S-transferase A1 level and hepatic biochemical indices(malondialdehyde, superoxide dismutase, glutathione and glutathione peroxidase) were determined. Pretreatment with FS extracts significantly inhibited the damage caused by ethanol and the hepatoprotective effects of FS were almost similar to Silymarin that was used to treat alcoholic liver injury. GSTA1 contents in all the FS extract-treated groups were significantly different from those in the ethanol-induced acute liver injury model group(p<0.01), and similar trends were observed in transaminases and hepatic indices level both in vitro and in vivo. The results showed that FS extracts had hepatoprotective effects against ethanol-induced injury. Those effects might be related to the enhancement of antioxidant capacity of liver cells, and FS extracts could reduce the release of liver GSTA1, which contributed to improve liver detoxification. 展开更多
关键词 ETHANOL Folium syringae extract GSTA1 liver injury primary hepatocyte MOUSE
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Identification of QTLs Associated with Resistance to Pseudomonas syringae pv.Glycinea in Soybean(Glycine max(L.)Merr)
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作者 Mei Hong-yao Liu Yang +2 位作者 Pan Xiao-cheng Su An-yu Wu Xiao-xia 《Journal of Northeast Agricultural University(English Edition)》 CAS 2021年第2期1-14,共14页
Soybean bacterial spot disease caused by Pseudomonas syringae pv.Glycinea which is a bacterial disease seriously affects soybean yield.Ten soybean germplasms and recombinant inbred lines(RILs)population were used to i... Soybean bacterial spot disease caused by Pseudomonas syringae pv.Glycinea which is a bacterial disease seriously affects soybean yield.Ten soybean germplasms and recombinant inbred lines(RILs)population were used to identify the resistant trait after inoculated with P.sg(P.sgneau001)in this study.High-density genetic mapping was obtained by specific length amplified fragment sequencing(SLAF-seq)of 149 RILs population which was derived from the crossing between Charleston and Dongnong594.The results indicated that 10 germplasm resources had four resistant germplasms included highly resistant cultivar Charleston,four susceptible varieties included Dongnong594 and two moderately resistant cultivars.Five quantitative trait locus(QTLs)were detected in RILs population by the composite interval mapping(CIM)method,and located on Linkage Group(LG)D1b(chromosome two),LG C2(chromosome six)and LG H(chromosome 12),respectively.LOD scores ranged from 2.68 to 4.95 and the phenotypic variation percentage was from 6%to 11%.Six candidate genes were detected,according to the result of gene annotation information.Four of them had relationship with protein kinase activity,protein phosphorylation and leucine rich repeat(LRR)transmembrane protein,which had high expression after inoculated with P.sg by qRT-PCR. 展开更多
关键词 SOYBEAN QTL mapping Pseudomonas syringae pv.Glycinea bacterial spot disease candidate gene
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Role of Coronatine in Inducing Infection and Improving Resistance to Pseudomonas syringae pv.mori in Mulberry Seedlings
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作者 Na WANG Fu'an WU 《Agricultural Biotechnology》 CAS 2017年第1期23-30,33,共9页
Mulberry bacterial blight is caused by Pseudomonas syringae pv. mori. Coronatine (COR), a phytotoxin and phytohormone produced by several strains of Pseudomonas syringae, is suggested to have an important role in pa... Mulberry bacterial blight is caused by Pseudomonas syringae pv. mori. Coronatine (COR), a phytotoxin and phytohormone produced by several strains of Pseudomonas syringae, is suggested to have an important role in pathogen-plant interaction. The aim of our study was to examine the influence of COR on mul- berry in the process of pathogen infection. Results showed that COR could suppress stomatal closure induced by pathogen-associated molecular patterns (PAMPS), assist pathogenic bacteria into the leaves, and promote bacterial proliferation in the tissues. High-concentration (1 μmol/L) exogenous COR and COR-producing bacterial pathogen induced chlorosis symptom and decrease of chlorophyll content, contrary to the effects of low-concentration (0.001 μmol/L) exogenous COR and non-COR-preducing bacterial pathogen. Treatments with COR and DC3000 enhanced the production of reactive oxygen species ( ROS), namely, hydrogen peroxide (H2O2 ) and superexide anion (O2-), but there were two H2O2 peaks at 1 -3 hpi and 8 -24 hpi in the DC3000-treated leaves while only one peak at 1 -3 h was observed 1 -3 h in the COR-treated ones. H202 could kill the pathogenic bacteria, on the other hand, it also acted as an upstream signaling molecule to promote nitric oxide (NO) production to further participate in the signaling pathway. Enzymatic antioxidant systems (superoxide dismutase, peroxidase and catalase) and defensive enzyme systems (lipoxygenase, polyphenol oxidase and phenylalnine ammonialyase) were activated by COR. Therefore, COR could cooperate on the inva- sion and proliferation of COR-producing bacterial pathogens, and induce the chloresis symptom in mulberry. At the same time, exogenously applied COR also could enhance the resistance to P. syringae pv. mori by production of signal molecules to activate signaling pathway and promoting defense-related metabolism. 展开更多
关键词 Pseudomonas syringae CORONATINE MULBERRY STOMA Reactive oxygen Enzyme activity
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Choline Promotes Growth and Tabtoxin Production in a <i>Pseudomonas syringae</i>Strain
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作者 Lucas A. Gallarato Emiliano D. Primo +1 位作者 ángela T. Lisa Mónica N. Garrido 《Advances in Microbiology》 2012年第3期327-331,共5页
Some Pseudomonas syringae pathovars secrete tabtoxin, a monocyclic β-lactam antibiotic, responsible for chlorosis, the principal halo blight symptom in susceptible plants as oats, rye, barley, wheat and sorghum, amon... Some Pseudomonas syringae pathovars secrete tabtoxin, a monocyclic β-lactam antibiotic, responsible for chlorosis, the principal halo blight symptom in susceptible plants as oats, rye, barley, wheat and sorghum, among other. Here, we demonstrated that the production of tabtoxin in a P. syringae strain increased at least 150%, when choline, betaine or dimethylglycine were used as nitrogen source, or when choline was added as osmoprotectant in hyperosmolar culture media. Besides, we investigated the induction of phosphorylcholine phosphatase (PchP) activity when choline or its metabolites were used as nitrogen sources. PchP is an enzyme involved in Pseudomonas aeruginosa pathogenesis through its contribution to the breakdown of choline-containing compounds of the host cells. Considering these results and that the success of a pathogenic microorganism depends on its ability to survive and proliferate in its target tissue, we propose that choline is one of the plant signals that contribute to establishment of the infection by tabtoxin-producing strains of P. syringae. 展开更多
关键词 CHOLINE Pseudomonas syringae PHYTOTOXINS PHOSPHORYLCHOLINE Phosphatase
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冰核细菌Pseudomonas syringae是否可以影响大气的冰核核化过程 被引量:6
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作者 王亚玲 杜睿 +1 位作者 梁宗敏 周宇光 《科学通报》 CAS CSCD 北大核心 2012年第25期2413-2418,共6页
近年来,大量的研究表明:生物气溶胶作为有效云凝结核与冰核在大气物理和化学过程中发挥着重要的作用.环境大气和云水样品中,冰核活性细菌Pseudomonas syringae的可培养菌株已经成功地被分离筛选出来.其在大气云物理过程尤其是降水过程... 近年来,大量的研究表明:生物气溶胶作为有效云凝结核与冰核在大气物理和化学过程中发挥着重要的作用.环境大气和云水样品中,冰核活性细菌Pseudomonas syringae的可培养菌株已经成功地被分离筛选出来.其在大气云物理过程尤其是降水过程中的重要作用也已经成为当前国际大气生物气溶胶气候效应研究的热点.本研究采用液滴冻结实验技术,测试了当前已被确定含有冰核活性菌的3种菌属即假单胞菌属(Pseudomonas)、欧文氏菌属(Erwinia)和黄单胞菌属(Xanthomonas)的代表性标准菌株(P.fluorescen,E.uredovora,X.campestris和P.syringaepv.panici),和被证实具有冰核活性的细菌菌株(P.syringaepv.lachrymans)的菌悬液液滴的冻结温度.结果显示:所测试的目前国际公认的冰核活性细菌属的标准菌株P.syringaepv.panici,P.fluorescen,E.uredovora,和X.campestris并不具备冰核活性,被测细菌菌悬液(OD600=0.25,数浓度108cells/mL)的液滴冻结温度分别是:20.3±2.3℃,21.8±2.3℃,20.8±3.4℃和19.9±3.3℃,与超纯水液滴的冻结温度(20.8±2.7℃)差异不大;而相同浓度下,P.syringaepv.lachrymans(ps1-10)菌株的冻结温度是5.0±0.8℃,具有显著的冰核活性.ps1-10菌株液滴平均冻结温度随着菌悬液浓度梯度降低的变化特征表明,当菌液浓度低于105cells/mL时,菌液液滴冻结温度明显降低,当菌液浓度低于104cells/mL时,菌液液滴与无冰核活性的液滴冻结温度接近.由此本文提出疑问:冰核细菌P.syringae是否能以浸润核化活性在大气真实环境中的冰核异质核化过程中起到重要作用,还是存在其他的作用机制? 展开更多
关键词 生物气溶胶 冰核活性 冰核活性细菌 PSEUDOMONAS syringae 冻结温度
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Natural Variation in Tomato Reveals Differences in the Recognition of AvrPto and AvrPtoB Effectors from Pseudomonas syringae 被引量:2
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作者 Christine M. Kraus Kathy R. Munkvold Gregory B. Martin 《Molecular Plant》 SCIE CAS CSCD 2016年第5期639-649,共11页
The Pto protein kinase from Solanum pimpinellifolium interacts with Pseudomonas syringae effectors AvrPto or AvrPtoB to activate effector-triggered immunity. The previously solved crystal structures of the AvrPto-Pto ... The Pto protein kinase from Solanum pimpinellifolium interacts with Pseudomonas syringae effectors AvrPto or AvrPtoB to activate effector-triggered immunity. The previously solved crystal structures of the AvrPto-Pto and AvrPtoB-Pto complexes revealed that Pro binds each effector through both a shared and a unique interface. Hera we use natural variation in wild species of tomato to further investigate Pto recognition of these two effectors. One species, Solanum chmielewskU, was found to have many accessions that recognize only AvrPtoB. The Pto ortholog from one of these accessions was responsible for recognition of AvrPtoB and it differed from Solanum pimpinellifolium Pto by only 14 amino acids, including two in the AvrPto-specific interface, glutamate-49/glycine-51. Converting these two residues to those in Pro (histidine-49/valine-51) did not restore recognition of AvrPto. Subsequent experiments revealed that a single substitution of a histidine-to-aspartate at position 193 in Pto, which is not near the AvrPto- specific interface, was sufficient for conferring recognRion of AvrPto in plant cells. The reciprocal substi- tution of aspartate-to-histidine-193 in Pto abolished AvrPto recognition, confirming the importance of this residue. Our results reveal new aspects about effector recognition by Pto and demonstrate the value of using natural variation to understand the interaction between resistance proteins and pathogen effectors. 展开更多
关键词 Effector-triggered immunity natural variation plant immunity Pseudomonas syringae PTO TOMATO
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Two plant NLR proteins confer strain-specific resistance conditioned by an effector from Pseudomonas syringae pv.actinidiae 被引量:1
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作者 Xiaojuan Zheng Zhaoyang Zhou +10 位作者 Zhen Gong Meijuan Hu Ye Jin Ahn Xiaojuan Zhang Yan Zhao Guoshu Gong Jian Zhang Jianru Zuo Guan-Zhu Han Sohn Kee Hoon Jian-Min Zhou 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2022年第8期823-832,共10页
Pseudomonas syringae pv.actinidiae(Psa)causes bacterial canker,a devastating disease threatening the Actinidia fruit industry.In a search for non-host resistance genes against Psa,we find that the nucleotidebinding le... Pseudomonas syringae pv.actinidiae(Psa)causes bacterial canker,a devastating disease threatening the Actinidia fruit industry.In a search for non-host resistance genes against Psa,we find that the nucleotidebinding leucine-rich repeat receptor(NLR)protein ZAR1 from both Arabidopsis and Nicotiana benthamiana(Nb)recognizes Hop Z5 and triggers cell death.The recognition requires ZED1 in Arabidopsis and JIM2 in Nb plants,which are members of the ZRK pseudokinases and known components of the ZAR1 resistosome.Surprisingly,Arabidopsis ZAR1 and RPM1,another NLR known to recognize Hop Z5,confer disease resistance to Hop Z5 in a strain-specific manner.Thus,ZAR1,but not RPM1,is solely required for resistance to P.s.maculicola ES4326(Psm)carrying hop Z5,whereas RPM1 is primarily required for resistance to P.s.tomato DC3000(Pst)carrying hop Z5.Furthermore,the ZAR1-mediated resistance to Psm hop Z5 in Arabidopsis is insensitive to SOBER1,which encodes a deacetylase known to suppress the RPM1-mediated resistance to Pst hop Z5.In addition,hop Z5 enhances P.syringae virulence in the absence of ZAR1 or RPM1 and that SOBER1 abolishes such virulence function.Together the study suggests that ZAR1 may be used for improving Psa resistance in Actinidia and uncovers previously unknown complexity of effectortriggered immunity and effector-triggered virulence. 展开更多
关键词 NLR ZAR1 Pseudomonas syringae pv.actinidiae IMMUNITY Disease resistance
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Establishment of a Loop-mediated Isothermal Amplification Method for Rice Bacterial Leaf Brown Spot Disease
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作者 Zhang Jun-hua Wang Liang +8 位作者 Zhang Yao Ni Zhe Xu Xiao-feng Yang Ming-xiu Peng Li-li Yang Xin Wang Yi-han Jiang Xiao-jiao Haseeb Younis 《Journal of Northeast Agricultural University(English Edition)》 CAS 2023年第1期13-19,共7页
Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to es... Rice bacterial leaf brown spot disease caused by Pseudomonas syringae pv.syringae(Pss)is a major disease on rice.In recent years,Pss has emerged worldwide,seriously affecting rice production.It is very important to establish a rapid detection method of Pss for the diagnosis and prevention of this disease.In order to robust and accurately diagnose the rice bacterial leaf brown spot disease in the field and laboratory,an assay system for the Pss was developed in this study,and the specific sequence of hrcN was used as the target,based on loop-mediated isothermal amplification(LAMP).The best detection system was MgSO 48 mmol·L^(-1),Bst DNA polymerase 8 U,dNTP 1.4 mmol·L^(-1),the ratio of internal and outer primers was 2:1,the reaction temperature was 63℃,the reaction time was 45 min,and the lowest sensitivity was 104 CFU·mL^(-1).This results provided an accurate and robust method for laboratory and field diagnosis of bacterial leaf brown spot disease of rice. 展开更多
关键词 Pseudomonas syringae pv.syringae loop-mediated isothermal amplification a rapid detection method
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Integrated Green Prevention and Control Techniques for Kiwifruit Canker Disease in "Guichang" Kiwifruit in Xiuwen County, Guizhou Province
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作者 Miao LI Xian WEI +4 位作者 Rong WU Shouying TANG Suran WAN Liqian JIANG Song BAI 《Agricultural Biotechnology》 CAS 2023年第5期30-34,42,共6页
Kiwifruit canker disease seriously affects the yield and quality of"Guichang"kiwifruit in Xiuwen County,Guizhou Province.In order to scientifically,safely,greenly and efficiently prevent and control the dise... Kiwifruit canker disease seriously affects the yield and quality of"Guichang"kiwifruit in Xiuwen County,Guizhou Province.In order to scientifically,safely,greenly and efficiently prevent and control the disease,theory was combined with prevention and control techniques to optimize existing prevention and control techniques,so as to improve the production yield and quality of kiwifruit.Specifically,biocontrol strains targeting local kiwifruit canker disease were screened,and reduced and mixed use of agrochemicals with improved efficiency was studied;and the effects and application techniques of disease resistance inducers and bioorganic fertilizers in inducing systemic disease resistance in kiwifruit trees were explored,and finally,an integrated green prevention and control scheme for kiwifruit canker disease that is suitable for kiwifruit production areas in Guizhou Province and has strong operability was proposed.This study provides technical support for green,efficient,standardized production technical services and sustainable and healthy development of kiwifruit industry. 展开更多
关键词 KIWI Pseudomonas syringae pv.actinidiae(Psa) SYMPTOM Occurrence rule Integrated control
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拟南芥不同ROP蛋白对病原细菌增殖的影响 被引量:5
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作者 王爱荣 陈新 +3 位作者 张冬梅 陈惠红 鲁国东 王宗华 《福建农林大学学报(自然科学版)》 CSCD 北大核心 2008年第6期610-613,共4页
ROP蛋白是植物特有的一类小G蛋白,在植物信号传导途径中起重要作用.拟南芥共编码11种ROP蛋白,为明确ROP蛋白在拟南芥抗病反应中的作用,将病原细菌Pseudomonas syringaepv.tomatoDC3000接种于各AtROP的激活和失活突变体后,观察其增殖情况... ROP蛋白是植物特有的一类小G蛋白,在植物信号传导途径中起重要作用.拟南芥共编码11种ROP蛋白,为明确ROP蛋白在拟南芥抗病反应中的作用,将病原细菌Pseudomonas syringaepv.tomatoDC3000接种于各AtROP的激活和失活突变体后,观察其增殖情况.结果表明,AtROP2和AtROP11抑制病原菌的增殖,而AtROP10则促进病原菌的增殖,其他At-ROP对Pst.DC3000的增殖没有影响. 展开更多
关键词 ROP蛋白 拟南芥 抗病性 PSEUDOMONAS syringae pv.tomato DC3000
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猕猴桃细菌性溃疡病研究进展 被引量:8
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作者 王涛 张计育 +3 位作者 王刚 贾展慧 潘德林 郭忠仁 《中国农学通报》 2020年第3期123-128,共6页
笔者总结了近年来国内外学者对猕猴桃细菌性溃疡病的研究和发现,详细介绍了猕猴桃细菌性溃疡病的症状、危害和传播途径以及致病菌Pseudomonas syringae pv.actinidiae的特征、分类和起源,分析了其可能的致病机理,并总结了猕猴桃的抗病... 笔者总结了近年来国内外学者对猕猴桃细菌性溃疡病的研究和发现,详细介绍了猕猴桃细菌性溃疡病的症状、危害和传播途径以及致病菌Pseudomonas syringae pv.actinidiae的特征、分类和起源,分析了其可能的致病机理,并总结了猕猴桃的抗病性研究。基于目前对猕猴桃细菌性溃疡病的防治方法和相关研究发现,展望了猕猴桃细菌性溃疡病的研究方向和防治措施,提出采取预防致病菌传入、增强树势和药剂防治相结合的防治措施,为以后的研究和生产实践提供理论依据。 展开更多
关键词 猕猴桃 溃疡病 PSEUDOMONAS syringae pv.actinidiae 致病机理 抗病性
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Construction of Lescpth5 Bait Vector and Detection of Its Self-activated Activity
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作者 张月娟 万一 +2 位作者 陈华民 张琨 赵廷昌 《Plant Diseases and Pests》 CAS 2011年第4期1-2,5,共3页
[ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connec... [ Objective ] The paper was to construct bait vector pGBKTT-Lescpth5, and detect its self-activated transcription activity and the toxicity on yeast cells. [ Method] Lescpth5 was amplified by PCR technique, and connected into bait vector pGBKTT. The recombined vector was transformed into yeast competent ceils AH109 and carried out self-activated detection and toxicity detection in the au.xotrephic medium. [ Result] Digestion and sequencing results showed that bait vector pGBKT7-Lescpth5 was suecessfully constructed with correct reading frame. Self-activated activity and toxicity detection results showed that bait vector had no self- activated activity on yeast strain AH109, which also had no toxicity on yeast. [Condusion] Bait vector successfully constructed could be used in yeast two-hybrid system, which laid the foundation for screening of cDNA library in the next step. 展开更多
关键词 Pseudomonas syringae pv. tomato Bait vector Yeast two-hybrid Self-activated China
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