[Objectives]This trial was conducted to investigate the effects of diet with"Lianteng"additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chic...[Objectives]This trial was conducted to investigate the effects of diet with"Lianteng"additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chickens.[Methods]480 Guangxi Sanhuang chickens at the age of 1 d were divided into 4 groups randomly and each group was assigned to 6 replicates with 20 chicks in each replicate;groups A,B and C were treatment groups and fed basal diets with 0.1%,0.2%and 0.4%of"Lianteng"additive of Chinese herbal medicine respectively during a trial of 70 d.Group D was control group and fed only basal diet.The serum samples were taken from 6 chicks in each group at the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in serum.[Results]Results from the trial showed that no difference was found in serum content of GLU,TG,TP,TC,and UN in the measurement at the age of 21,35 and 49 d(P>0.05);however,the content of ALB from groups A and B at 35 d was significantly higher than that from group D(P<0.01),and the ALB content from group B at 49 d was also higher than that from group D(P<0.05);the IgM content at 49 d from group A was lower than that from group D(P<0.05),in addition to this,there was no differences found in the contents of IgG,IgM and IFN-y among groups A,B,C and D during the trial(P>0.05);but the content of IL-2 from groups A and B at 49 d was significantly greater than that from group D(P<0.05);T-AOC from groups A,B and C at 49 d was very significantly higher than that from group D(P<0.01),and the one of group B at 35 d was still obviously higher than that of group D(P<0.05);also at 49 d,the GSH content in groups A and B was much greater than that in group D(P<0.01);T-SOD activity of group B and C at 35 d was distinctly greater than that of group D(P<0.05).[Conclusions]It can be concluded that the addition of 0.2%of"Lianteng"additive of Chinese herbal medicine in diet can increase immune function in a certain extent and promote the antioxidant ability observably for Guangxi Sanhuang chickens.展开更多
[Objective] To compare immune efficacy of avian influenza virus (AIV) Re-5 strain (H5N1 subtype) and SS strain (H9 subtype) in Guangxi Sanhuang chickens. [Method] A total of 180 Sanhuang chickens were selected from a ...[Objective] To compare immune efficacy of avian influenza virus (AIV) Re-5 strain (H5N1 subtype) and SS strain (H9 subtype) in Guangxi Sanhuang chickens. [Method] A total of 180 Sanhuang chickens were selected from a breeding chicken farm in Guilin City of Guangxi and assigned into four groups (n = 45). At 145 days old, the chickens in the group I and II were respectively inoculated with AIV Re-5 strain and AIV SS strain, but they were kept in cages separately. The two control groups were not inoculated. Antibody levels were determined at 170 days old. [Result] The level of antibodies against AIV Re-5 strain was significantly higher in the chickens of the group I and group II than in the corresponding control groups, respectively (P < 0.01). The level of antibodies against AIV SS strain was not significantly different between the group I and its control group or between the group II and its control group. [Conclusion] The antibody level of chickens can be increased by the AIV Re-5 strain but not by the AIV SS strain.展开更多
Objective: To observe the effect of Sanhuang Jiangtang recipe (三黄降糖, SAT) on insulinperipheral resistance in Type Ⅱdiabetes mellitus (DM). Methods:Ninety-five patients with type Ⅱ DM wererandomly divided into tw...Objective: To observe the effect of Sanhuang Jiangtang recipe (三黄降糖, SAT) on insulinperipheral resistance in Type Ⅱdiabetes mellitus (DM). Methods:Ninety-five patients with type Ⅱ DM wererandomly divided into two groups. Fifty-three cases of SHJT group were given decoction and tablets of SAT orally for 4 -- 6 months. The efficacy was compared with that of 42 cases treated with Glipizide as the control.Before and after treatment standard steamed bread meal test was performed to measure the insulin peripheralsensitivity, insulin release to glucose and insulin sensitivity index. Results: (1 ) The total effective rates of improving insulin peripheral resistance and reducing blood sugar in SHJT group were 79. 2 % and 80. I %, whichwas equivalent to levels in the control group, but SAT recipe was more effective in relieving symptoms of Qideficiency and signs of blood stasis. (2) In SalT group, the insulin peripheral sensitivity and insulin sensitivityindex were significantly increased (P < 0. 05 and 0. of ), meanwhile the fasting blood sugar and blood sugar areawere reduced (P < 0. 05), but the change of insulin release to glucose was blunted. (3 )The lowering of bloodsugar in SHJT group was significantly negative correlated with the changing of degree of insulin peripheral sensitivity and index of insulin sensitivity (P < 0. 01 and 0. 05), but not with that of insulin area. Conclusion: Itsuggested that the treatment of SHJT recipe might decrease insulin peripheral resistance (partial reversal) bymeans of reducing hyperinsulinemia and improving insulin sensitivity.展开更多
OBJECTIVE: To determine the effectiveness of modified Sanhuang Xiexin Tang(SHXXT) plus additional herbs(MSAH) combined with "standard triple therapy" for eradication of Helicobacter pylori(H. pylori) and ame...OBJECTIVE: To determine the effectiveness of modified Sanhuang Xiexin Tang(SHXXT) plus additional herbs(MSAH) combined with "standard triple therapy" for eradication of Helicobacter pylori(H. pylori) and amelioration of related symptoms in comparison with standard triple and standard quadruple therapies.METHODS: From October 2015 to May 2016, we recruited patients with dyspepsia symptoms confirmed to have H. pylori infection by the13 C urea breath test in our outpatient clinic. Patients were randomly divided into three treatment groups: Nexium? standard triple therapy(group A; EAC); Nexium? standard quadruple therapy(group B; EBAC);or Nexium? standard triple therapy combined with MSAH(group C; EAC+MSAH). Comparisons of H. pylori eradication and symptom amelioration rates were made among the three groups at 2 or 6 weeks after group assignment.RESULTS: There was no difference in H. pylori eradi-cation rates between groups B(EBAC) and C(EAC+MSAH)(P = 0.486), and eradication rates in groups B and C were significantly higher than that in group A(EAC)(PA vs B= 0.001; PA vs C= 0.003). There was no difference in the total symptom score among the groups before treatment. In all groups,the total symptom scores after treatment(2 or 6 weeks after group assignment) were significantly lower than those before treatment(P < 0.001 for all). However, group C(EAC + MSAH) demonstrated superior total symptom scores and symptom amelioration rates than groups A(EAC) and B(EBAC).Group B also demonstrated better scores and rates than group A. There was no difference in symptom amelioration rates at 2 and 6 weeks within each group.CONCLUSION: There is no difference between MSAH combined with standard triple therapy and standard quadruple therapy containing bismuth with regard to H. pylori eradication rate. However,MSAH combined with standard triple therapy has a higher symptom amelioration rate and therefore appears to be an ideal treatment scheme for H. pylori eradication.展开更多
OBJECTIVE:To study the effect of methotrexate(MTX) combined with Sanhuang Yilong decoction(SYD) on aquaporin(AQP) expression,and to explore the role of AQPs in the pathogenesis of rheumatoid arthritis(RA).METHODS:A to...OBJECTIVE:To study the effect of methotrexate(MTX) combined with Sanhuang Yilong decoction(SYD) on aquaporin(AQP) expression,and to explore the role of AQPs in the pathogenesis of rheumatoid arthritis(RA).METHODS:A total of 118 dampness-heat blockage type RA patients who were hospitalized in the General Chengdu Military Hospital between January2014 and December 2016 were selected as subjects in this study(30 patient of these patients with knee joint effusion were assigned to the RA synovial fluid group).For the pre-treatment controlgroups,30 healthy volunteers were recruited as the healthy control group and 30 osteoarthritis(OA) patients with knee joint effusion were included as OA synovial fluid control group.The RA dampnessheat blockage syndrome treatment groups were divided into 45 cases in the combined group and 45 cases in the MTX group.The combined group received MTX combined with SYD treatment while the MTX group received MTX alone.AQP1,AQP2 and AQP3 expressions were detected in the serum and synovial fluid.RESULTS:AQP1 had the highest expression,followed by AQP3,and AQP2.The serum levels of AQP1,AQP2 and AQP3 were all significantly lower than those in the healthy volunteers(P < 0.05),while the synovial fluid AQP1,AQP2 and AQP3 expression in the RA group were comparable to these in the OA groups(P > 0.05).After treatment for2 weeks,serum AQP1,AQP2,AQP3 were significantly increased and erythrocyte sedimentation rate,C-reactive protein,disease activity score of 28 joints were decreased in the combined group(P < 0.05).CONCLUSION:Abnormal expression of AQPs inhibits water metabolism in RA dampness-heat blockage syndrome,so liquid is accumulated at the joint,which may play an important role in the pathogenesis of RA.MTX combined with SYD for the treatment of RA can effectively increase AQP expression.展开更多
基金Supported by the Special Project of Basic Scientific Research Business Expenses of Public Welfare Scientific Research Institutes Directly under Guangxi District(Gui Mu Yan Ke 2019-9)Guangxi Innovation Team Construction Project of National Modern Agricultural Industrial Technology System(nycytxgxcxtd).
文摘[Objectives]This trial was conducted to investigate the effects of diet with"Lianteng"additive of Chinese herbal medicine on blood biochemical indexes,immune and antioxidant function of Guangxi Sanhuang chickens.[Methods]480 Guangxi Sanhuang chickens at the age of 1 d were divided into 4 groups randomly and each group was assigned to 6 replicates with 20 chicks in each replicate;groups A,B and C were treatment groups and fed basal diets with 0.1%,0.2%and 0.4%of"Lianteng"additive of Chinese herbal medicine respectively during a trial of 70 d.Group D was control group and fed only basal diet.The serum samples were taken from 6 chicks in each group at the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in serum.[Results]Results from the trial showed that no difference was found in serum content of GLU,TG,TP,TC,and UN in the measurement at the age of 21,35 and 49 d(P>0.05);however,the content of ALB from groups A and B at 35 d was significantly higher than that from group D(P<0.01),and the ALB content from group B at 49 d was also higher than that from group D(P<0.05);the IgM content at 49 d from group A was lower than that from group D(P<0.05),in addition to this,there was no differences found in the contents of IgG,IgM and IFN-y among groups A,B,C and D during the trial(P>0.05);but the content of IL-2 from groups A and B at 49 d was significantly greater than that from group D(P<0.05);T-AOC from groups A,B and C at 49 d was very significantly higher than that from group D(P<0.01),and the one of group B at 35 d was still obviously higher than that of group D(P<0.05);also at 49 d,the GSH content in groups A and B was much greater than that in group D(P<0.01);T-SOD activity of group B and C at 35 d was distinctly greater than that of group D(P<0.05).[Conclusions]It can be concluded that the addition of 0.2%of"Lianteng"additive of Chinese herbal medicine in diet can increase immune function in a certain extent and promote the antioxidant ability observably for Guangxi Sanhuang chickens.
基金funded by the Program for Excellent Talents in Guangxi Higher Education Institutions (Guilin Education Talents 0857)the Fourth Project of the 11th Five-Year Plan for Guangxi New Century Education Reform Engineering(Guilin Higher Education[2008]No.178)
文摘[Objective] To compare immune efficacy of avian influenza virus (AIV) Re-5 strain (H5N1 subtype) and SS strain (H9 subtype) in Guangxi Sanhuang chickens. [Method] A total of 180 Sanhuang chickens were selected from a breeding chicken farm in Guilin City of Guangxi and assigned into four groups (n = 45). At 145 days old, the chickens in the group I and II were respectively inoculated with AIV Re-5 strain and AIV SS strain, but they were kept in cages separately. The two control groups were not inoculated. Antibody levels were determined at 170 days old. [Result] The level of antibodies against AIV Re-5 strain was significantly higher in the chickens of the group I and group II than in the corresponding control groups, respectively (P < 0.01). The level of antibodies against AIV SS strain was not significantly different between the group I and its control group or between the group II and its control group. [Conclusion] The antibody level of chickens can be increased by the AIV Re-5 strain but not by the AIV SS strain.
文摘Objective: To observe the effect of Sanhuang Jiangtang recipe (三黄降糖, SAT) on insulinperipheral resistance in Type Ⅱdiabetes mellitus (DM). Methods:Ninety-five patients with type Ⅱ DM wererandomly divided into two groups. Fifty-three cases of SHJT group were given decoction and tablets of SAT orally for 4 -- 6 months. The efficacy was compared with that of 42 cases treated with Glipizide as the control.Before and after treatment standard steamed bread meal test was performed to measure the insulin peripheralsensitivity, insulin release to glucose and insulin sensitivity index. Results: (1 ) The total effective rates of improving insulin peripheral resistance and reducing blood sugar in SHJT group were 79. 2 % and 80. I %, whichwas equivalent to levels in the control group, but SAT recipe was more effective in relieving symptoms of Qideficiency and signs of blood stasis. (2) In SalT group, the insulin peripheral sensitivity and insulin sensitivityindex were significantly increased (P < 0. 05 and 0. of ), meanwhile the fasting blood sugar and blood sugar areawere reduced (P < 0. 05), but the change of insulin release to glucose was blunted. (3 )The lowering of bloodsugar in SHJT group was significantly negative correlated with the changing of degree of insulin peripheral sensitivity and index of insulin sensitivity (P < 0. 01 and 0. 05), but not with that of insulin area. Conclusion: Itsuggested that the treatment of SHJT recipe might decrease insulin peripheral resistance (partial reversal) bymeans of reducing hyperinsulinemia and improving insulin sensitivity.
文摘OBJECTIVE: To determine the effectiveness of modified Sanhuang Xiexin Tang(SHXXT) plus additional herbs(MSAH) combined with "standard triple therapy" for eradication of Helicobacter pylori(H. pylori) and amelioration of related symptoms in comparison with standard triple and standard quadruple therapies.METHODS: From October 2015 to May 2016, we recruited patients with dyspepsia symptoms confirmed to have H. pylori infection by the13 C urea breath test in our outpatient clinic. Patients were randomly divided into three treatment groups: Nexium? standard triple therapy(group A; EAC); Nexium? standard quadruple therapy(group B; EBAC);or Nexium? standard triple therapy combined with MSAH(group C; EAC+MSAH). Comparisons of H. pylori eradication and symptom amelioration rates were made among the three groups at 2 or 6 weeks after group assignment.RESULTS: There was no difference in H. pylori eradi-cation rates between groups B(EBAC) and C(EAC+MSAH)(P = 0.486), and eradication rates in groups B and C were significantly higher than that in group A(EAC)(PA vs B= 0.001; PA vs C= 0.003). There was no difference in the total symptom score among the groups before treatment. In all groups,the total symptom scores after treatment(2 or 6 weeks after group assignment) were significantly lower than those before treatment(P < 0.001 for all). However, group C(EAC + MSAH) demonstrated superior total symptom scores and symptom amelioration rates than groups A(EAC) and B(EBAC).Group B also demonstrated better scores and rates than group A. There was no difference in symptom amelioration rates at 2 and 6 weeks within each group.CONCLUSION: There is no difference between MSAH combined with standard triple therapy and standard quadruple therapy containing bismuth with regard to H. pylori eradication rate. However,MSAH combined with standard triple therapy has a higher symptom amelioration rate and therefore appears to be an ideal treatment scheme for H. pylori eradication.
基金the Army Medical Research Twelfth Five Year Plan Key Project(No.BWS11J06)the Sichuan Provincial Health Department(No.120573)
文摘OBJECTIVE:To study the effect of methotrexate(MTX) combined with Sanhuang Yilong decoction(SYD) on aquaporin(AQP) expression,and to explore the role of AQPs in the pathogenesis of rheumatoid arthritis(RA).METHODS:A total of 118 dampness-heat blockage type RA patients who were hospitalized in the General Chengdu Military Hospital between January2014 and December 2016 were selected as subjects in this study(30 patient of these patients with knee joint effusion were assigned to the RA synovial fluid group).For the pre-treatment controlgroups,30 healthy volunteers were recruited as the healthy control group and 30 osteoarthritis(OA) patients with knee joint effusion were included as OA synovial fluid control group.The RA dampnessheat blockage syndrome treatment groups were divided into 45 cases in the combined group and 45 cases in the MTX group.The combined group received MTX combined with SYD treatment while the MTX group received MTX alone.AQP1,AQP2 and AQP3 expressions were detected in the serum and synovial fluid.RESULTS:AQP1 had the highest expression,followed by AQP3,and AQP2.The serum levels of AQP1,AQP2 and AQP3 were all significantly lower than those in the healthy volunteers(P < 0.05),while the synovial fluid AQP1,AQP2 and AQP3 expression in the RA group were comparable to these in the OA groups(P > 0.05).After treatment for2 weeks,serum AQP1,AQP2,AQP3 were significantly increased and erythrocyte sedimentation rate,C-reactive protein,disease activity score of 28 joints were decreased in the combined group(P < 0.05).CONCLUSION:Abnormal expression of AQPs inhibits water metabolism in RA dampness-heat blockage syndrome,so liquid is accumulated at the joint,which may play an important role in the pathogenesis of RA.MTX combined with SYD for the treatment of RA can effectively increase AQP expression.
