Objective To study the drug effects of “Caulis Sargentodoxae Formula” which clearsaway heat and blood clot on experimental endometriosis in rat, and to compare thiseffect with Danazol’s effects.Method The model of ...Objective To study the drug effects of “Caulis Sargentodoxae Formula” which clearsaway heat and blood clot on experimental endometriosis in rat, and to compare thiseffect with Danazol’s effects.Method The model of endometriosis rat was induced by transplanting with endometriumsurgically. The rats were divided into 6 groups randomly: the non-treatment group, thecastrate group, Danazol group(80 mg/kg), “Caulis Sargentodoxae Formula” group(110 g/kg), the medium dose group(77.5 g/kg) and the low dose group(55 g/kg). After3 weeks’ treatment, the volume of endometrium was detected by cutting the rats’ bellyopen, drawing the materials from endometrium for tissue section, and doing the quantityanalysis of endometrium by semiautomatic image analysis machine.Results There was no significant difference of the endometrium volumes between thelow dose group and the non-treatment group(P>0.05), other groups’ endometriumvolumes were significantly lower than those of the non-treatment group(P<0.05). Andapart from the low dose group, all other groups’ heights of the endometrium epitheliawere significantly lower than that of the non-treatment group(p<0.01).Conclusion “Caulis Sargentodoxae Formula” can refrain the growth of theendometrium, and the effects are better than that of Danazol’s.展开更多
Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who ...Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.展开更多
目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑...目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。展开更多
The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-pea...The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-peak pattern. The first peak appeared at June and the second at September. The seasonal dynamic change of flavonoids in leafstalk, annual twig and perennial stem were different and inconspicuous. The flavonoid cumulated maximally in the leafblade and the followed was seccessively annual twig, leafstalk, and perennial stem. The flavonoid content in leafblade was 31.79 mg/g(DW), while that in annual twig, leafstalk and perennial stem was 5.41 mg/g(DW), 5.30 mg/g(DW) and 3.95 mg/g(DW) respectively. There was markedly higher flavonoid content in leafblade. It is considered that the leaf of Sargentodoxa cuneata is significant in further exploitation.展开更多
文摘Objective To study the drug effects of “Caulis Sargentodoxae Formula” which clearsaway heat and blood clot on experimental endometriosis in rat, and to compare thiseffect with Danazol’s effects.Method The model of endometriosis rat was induced by transplanting with endometriumsurgically. The rats were divided into 6 groups randomly: the non-treatment group, thecastrate group, Danazol group(80 mg/kg), “Caulis Sargentodoxae Formula” group(110 g/kg), the medium dose group(77.5 g/kg) and the low dose group(55 g/kg). After3 weeks’ treatment, the volume of endometrium was detected by cutting the rats’ bellyopen, drawing the materials from endometrium for tissue section, and doing the quantityanalysis of endometrium by semiautomatic image analysis machine.Results There was no significant difference of the endometrium volumes between thelow dose group and the non-treatment group(P>0.05), other groups’ endometriumvolumes were significantly lower than those of the non-treatment group(P<0.05). Andapart from the low dose group, all other groups’ heights of the endometrium epitheliawere significantly lower than that of the non-treatment group(p<0.01).Conclusion “Caulis Sargentodoxae Formula” can refrain the growth of theendometrium, and the effects are better than that of Danazol’s.
文摘Objective:To study the effect of retention enema by caulis sargentodoxae enema combined with oral drugs on the immune inflammatory response and intestinal flora of UC patients. Methods: A total of 128 UC patients who received treatment in the hospital between January 2013 and April 2017 were collected and divided into control group and observation group by random number table. Control group received conventional oral drug therapy, and the observation group received retention enema by caulis sargentodoxae enema combined with oral drug therapy. Before treatment and after 4 weeks of treatment, the differences in the levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were compared between the two groups.Results: The differences in levels of immunoglobulin and inflammatory response indexes in serum as well as intestinal flora levels in mucosa lesion tissue were not statistically significant between the two groups of patients before treatment. After 4 weeks of treatment, IgA, IgG, IgM and IL-4 contents in serum as well as bifidobacterium and lactobacillus levels in mucosa lesion tissue of observation group were higher than those of control group while ESR, CRP, IL-6 and TNF-αcontents as well as E. coli level in mucosa lesion tissue were lower than those of control group. Conclusion: Retention enema by caulis sargentodoxae enema combined with oral drugs can effectively optimize the immune inflammatory state and balance the intestinal flora distribution in patients with UC.
文摘目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。
文摘The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-peak pattern. The first peak appeared at June and the second at September. The seasonal dynamic change of flavonoids in leafstalk, annual twig and perennial stem were different and inconspicuous. The flavonoid cumulated maximally in the leafblade and the followed was seccessively annual twig, leafstalk, and perennial stem. The flavonoid content in leafblade was 31.79 mg/g(DW), while that in annual twig, leafstalk and perennial stem was 5.41 mg/g(DW), 5.30 mg/g(DW) and 3.95 mg/g(DW) respectively. There was markedly higher flavonoid content in leafblade. It is considered that the leaf of Sargentodoxa cuneata is significant in further exploitation.
