[Objective] The paper was to explore the effective agent against seedling blight of Atractylodes macrocephala. [Method] The control effects of 60% validamycin A SP against seeding blight of A. macrocephala were studie...[Objective] The paper was to explore the effective agent against seedling blight of Atractylodes macrocephala. [Method] The control effects of 60% validamycin A SP against seeding blight of A. macrocephala were studied in the field, and compared with that of control agent 20% bismerthiazol WP. [Result] At 14 d post the third spraying, the control effect of 60% validamycin A SP at the dose of 495-540 g/hm;was 76.9%,which was equivalent to that of control agent 20% bismerthiazol WP sprayed at the dose of 270 g/hm;（76.7%）. [Conclusion] 60% Validamycin A SP could be used as the ideal agent against seedling blight of A. macrocephala, with good prospects of application and popularization.展开更多
Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well understood.In this study,GWAS performed by MLM and 3VmrMLM identi...Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well understood.In this study,GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs,associated with seedling blight resistance.These methods identified 49 and 36 genes,respectively.Functional verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with F.verticillioides,compared with the WT.The length of the stem rot lesion caused by F.verticillioides increased by 86%in mutant seedlings,and the relative length of the adult plant stalk rot increased by 35%in mutant plants compared to the wild type after inoculation with Fusarium graminearum.Transcriptome analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type,synthesis of secondary metabolites associated with resistance was reduced,and the immune response triggered by PAMP decreased,resulting in decreased resistance of mutant maize seedlings.Candidate gene association analysis showed that most maize inbred lines carried the susceptible haplotype.A functional PCR marker was developed.The results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.展开更多
Objective] This research almed to study technical requirements by seed breeding of Thuia sutchuenensis so as to Iay a foundation for the saving and breeding of Thuia sutchuenensis. [Method] The seeds of Thuia sutchuen...Objective] This research almed to study technical requirements by seed breeding of Thuia sutchuenensis so as to Iay a foundation for the saving and breeding of Thuia sutchuenensis. [Method] The seeds of Thuia sutchuenensis, coI-Iected from Xianyi Town, Chengkou County, Chongqing City, were used as material. The effects of temperature and matrix on germination rate and emergence rate of Thuia sutchuenensis were investigated. In addition, the controI effects of different-type and different-concentration pesticides on seedIing bIight of Thuia sutchuenensis were also studied. [Result] The suitabIe temperature for seed germination of Thuia sutchuenensis was 15-20 ℃, whiIe the optimum temperature was 20 ℃, under which the germination rate couId reach 95%. At temperature of 25 ℃, the seeds of Thuia sutchuenensis almost couId not germinate. The seeds of Thuia sutchuenensis germinated most if soaked in warm water of 40 ℃. After 24 h soaking, the germi-nated seeds were not increased significantIy. Among the matrixes for contalner seedIing, the 18th matrix, composed by sawdust （2/3） and forest topsoiI or general soiI （1/3）, was conducive to the seed germination in contalners. Among the pesti-cides, the controI effect of ferrous suIfate on diseases and pests in Thuia sutchue-nensis was best with cumuIative incidence of 15%, foI owed by those of fe-naminosuIf （17%）, Bordeaux mixture （21%）, KangkuIing （22%） and potassium per-manganate. [Conclusion] Sawdust had great effect on emergence rate of Thuia sutchuenensis in contalner seedIing, which might be reIated to good water retention and permeabiIity of sawdust. However, too much forest soiI was not conducive to the emergence rate of Thuia sutchuenensis in contalner seedIing. Ferrous suIfate had the best controI effect on diseases and pests in Thuia sutchuenensis.展开更多
文摘[Objective] The paper was to explore the effective agent against seedling blight of Atractylodes macrocephala. [Method] The control effects of 60% validamycin A SP against seeding blight of A. macrocephala were studied in the field, and compared with that of control agent 20% bismerthiazol WP. [Result] At 14 d post the third spraying, the control effect of 60% validamycin A SP at the dose of 495-540 g/hm;was 76.9%,which was equivalent to that of control agent 20% bismerthiazol WP sprayed at the dose of 270 g/hm;（76.7%）. [Conclusion] 60% Validamycin A SP could be used as the ideal agent against seedling blight of A. macrocephala, with good prospects of application and popularization.
基金supported by grants from the National Key Research and Development Program Project of China(2022YFD1201803)Research on Resistance Genetics of Maize Root Rot Disease,State Key Laboratory of Agronomy College,Henan Agricultural University,China(39990073/111)。
文摘Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well understood.In this study,GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs,associated with seedling blight resistance.These methods identified 49 and 36 genes,respectively.Functional verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with F.verticillioides,compared with the WT.The length of the stem rot lesion caused by F.verticillioides increased by 86%in mutant seedlings,and the relative length of the adult plant stalk rot increased by 35%in mutant plants compared to the wild type after inoculation with Fusarium graminearum.Transcriptome analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type,synthesis of secondary metabolites associated with resistance was reduced,and the immune response triggered by PAMP decreased,resulting in decreased resistance of mutant maize seedlings.Candidate gene association analysis showed that most maize inbred lines carried the susceptible haplotype.A functional PCR marker was developed.The results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.
文摘Objective] This research almed to study technical requirements by seed breeding of Thuia sutchuenensis so as to Iay a foundation for the saving and breeding of Thuia sutchuenensis. [Method] The seeds of Thuia sutchuenensis, coI-Iected from Xianyi Town, Chengkou County, Chongqing City, were used as material. The effects of temperature and matrix on germination rate and emergence rate of Thuia sutchuenensis were investigated. In addition, the controI effects of different-type and different-concentration pesticides on seedIing bIight of Thuia sutchuenensis were also studied. [Result] The suitabIe temperature for seed germination of Thuia sutchuenensis was 15-20 ℃, whiIe the optimum temperature was 20 ℃, under which the germination rate couId reach 95%. At temperature of 25 ℃, the seeds of Thuia sutchuenensis almost couId not germinate. The seeds of Thuia sutchuenensis germinated most if soaked in warm water of 40 ℃. After 24 h soaking, the germi-nated seeds were not increased significantIy. Among the matrixes for contalner seedIing, the 18th matrix, composed by sawdust （2/3） and forest topsoiI or general soiI （1/3）, was conducive to the seed germination in contalners. Among the pesti-cides, the controI effect of ferrous suIfate on diseases and pests in Thuia sutchue-nensis was best with cumuIative incidence of 15%, foI owed by those of fe-naminosuIf （17%）, Bordeaux mixture （21%）, KangkuIing （22%） and potassium per-manganate. [Conclusion] Sawdust had great effect on emergence rate of Thuia sutchuenensis in contalner seedIing, which might be reIated to good water retention and permeabiIity of sawdust. However, too much forest soiI was not conducive to the emergence rate of Thuia sutchuenensis in contalner seedIing. Ferrous suIfate had the best controI effect on diseases and pests in Thuia sutchuenensis.
