The aim of the present work is to assess the value of Detarium Senegalense by determining the content of total phenols, total flavonoids and total anthocyanins, and by evaluating the free radical scavenging activity o...The aim of the present work is to assess the value of Detarium Senegalense by determining the content of total phenols, total flavonoids and total anthocyanins, and by evaluating the free radical scavenging activity of Detarium Senegalense extracts. For this purpose, sequential extraction using solvents of increasing polarity was essential. The various extracts obtained underwent phytochemical and biochemical analyses. Phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, polyphenols, anthocyanins and steroids/terpenes. Quantitative analysis of total polyphenols, total flavonoids and total anthocyanins yielded the following results: total flavonoids (0.803 ± 0029 mg EQ/100g P for acetone extract of roots and 0.871 ± 0.401 mg EQ/100g P for methanol extract of leaves);total polyphenols (23.298 ± 12.68 mg EAG/100g P for acetone extract of roots and 24.69 ± 0.49 401 mg EAG/100g P for methanol extract of leaves);total monomeric anthocyanins (44.697 ± 0.939 mg EC3G/100g P and 16.699 ± 0.193 mg EC3G/100g P respectively for acetone and methanol extracts of stem bark). DPPH free radical scavenging activity was 1.674 ± 0.023 mg/mL for the acetone extract and 0.934 ± 0.24 mg/mL for the methanol extract of roots. .展开更多
Detarium senegalense J. F. Gmel., of the family of Fabaceae is one of the most exploited tree species in Togo. However, there are only few scientific studies on the distribution of this specie. This study is a contrib...Detarium senegalense J. F. Gmel., of the family of Fabaceae is one of the most exploited tree species in Togo. However, there are only few scientific studies on the distribution of this specie. This study is a contribution to the promotion of multi-purpose tree species in Togo with particular emphasis on a better knowledge of Detarium senegalense. It provides data on the ecology of the specie and his state of regeneration. The data on the specie are based on sample plots in the areas identified as hosts of the specie in Togo. A total of 77 circular plots of 1000 m2 (17.8 m radius) were established in ecological zones II, IV and V of Togo. The dendrometric parameters identified are: the total height and diameter at breast height (DBH) of Detarium senegalense and of all species found in each plot. Detarium senegalense trees of DBH < 10 cm are considered as potential regenerations. Detarium senegalense is more abundant in zones IV (forest area), followed by zone II. In zone V, Detarium senegalense is located only in the forest of “Have”. The structure of Detarium senegalense has a bell-shaped distribution with predominance of trees with large and intermediate diameters ranging between 30 and 70 cm. Trees of small DBH (Detarium senegalense occurs. This indicates a low regeneration rate of the specie. However, the structure of all species inventoried has a negative exponential distribution with predominance of small diameter trees. The sustainable management of Detarium senegalense in the forest can only be secured through a sustainable harvesting of seeds and actives promotion of natural regeneration.展开更多
Methoxylated flavonoids isolated from cold acetone leaf wash of Polygonum senegalense;5-hydroxy-7-methoxyflavanone 1 and 5-hydroxy-6,7-dimethoxy-flavanone 3, were tested for their ability to enhance thermal stability ...Methoxylated flavonoids isolated from cold acetone leaf wash of Polygonum senegalense;5-hydroxy-7-methoxyflavanone 1 and 5-hydroxy-6,7-dimethoxy-flavanone 3, were tested for their ability to enhance thermal stability of vegetable oils. Determination of the peroxide value (P.V.) and the p-Anisidine value (p-A.V.) was done according to the standard methods of analysis. The compounds were tested for in vitro cytotoxicity against a mammalian cell-line, Chinese Hamster Ovarian (CHO) using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazoliumbromide (MTT)-assay. Studies on changes in peroxide and p-Anisidine values for the oils heated to temperatures between 180°C and 200°C recorded better stability enhancement at 100 ppm concentration with these flavonoids than the commercial antioxidant, butylated hydroxytoluene (BHT). The plant-based flavonoids had no significant cytotoxic effect against the CHO cell-line and may serve as alternative antioxidants to synthetic ones which have previously raised great concern over the health of consumers.展开更多
文摘The aim of the present work is to assess the value of Detarium Senegalense by determining the content of total phenols, total flavonoids and total anthocyanins, and by evaluating the free radical scavenging activity of Detarium Senegalense extracts. For this purpose, sequential extraction using solvents of increasing polarity was essential. The various extracts obtained underwent phytochemical and biochemical analyses. Phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, polyphenols, anthocyanins and steroids/terpenes. Quantitative analysis of total polyphenols, total flavonoids and total anthocyanins yielded the following results: total flavonoids (0.803 ± 0029 mg EQ/100g P for acetone extract of roots and 0.871 ± 0.401 mg EQ/100g P for methanol extract of leaves);total polyphenols (23.298 ± 12.68 mg EAG/100g P for acetone extract of roots and 24.69 ± 0.49 401 mg EAG/100g P for methanol extract of leaves);total monomeric anthocyanins (44.697 ± 0.939 mg EC3G/100g P and 16.699 ± 0.193 mg EC3G/100g P respectively for acetone and methanol extracts of stem bark). DPPH free radical scavenging activity was 1.674 ± 0.023 mg/mL for the acetone extract and 0.934 ± 0.24 mg/mL for the methanol extract of roots. .
文摘Detarium senegalense J. F. Gmel., of the family of Fabaceae is one of the most exploited tree species in Togo. However, there are only few scientific studies on the distribution of this specie. This study is a contribution to the promotion of multi-purpose tree species in Togo with particular emphasis on a better knowledge of Detarium senegalense. It provides data on the ecology of the specie and his state of regeneration. The data on the specie are based on sample plots in the areas identified as hosts of the specie in Togo. A total of 77 circular plots of 1000 m2 (17.8 m radius) were established in ecological zones II, IV and V of Togo. The dendrometric parameters identified are: the total height and diameter at breast height (DBH) of Detarium senegalense and of all species found in each plot. Detarium senegalense trees of DBH < 10 cm are considered as potential regenerations. Detarium senegalense is more abundant in zones IV (forest area), followed by zone II. In zone V, Detarium senegalense is located only in the forest of “Have”. The structure of Detarium senegalense has a bell-shaped distribution with predominance of trees with large and intermediate diameters ranging between 30 and 70 cm. Trees of small DBH (Detarium senegalense occurs. This indicates a low regeneration rate of the specie. However, the structure of all species inventoried has a negative exponential distribution with predominance of small diameter trees. The sustainable management of Detarium senegalense in the forest can only be secured through a sustainable harvesting of seeds and actives promotion of natural regeneration.
文摘Methoxylated flavonoids isolated from cold acetone leaf wash of Polygonum senegalense;5-hydroxy-7-methoxyflavanone 1 and 5-hydroxy-6,7-dimethoxy-flavanone 3, were tested for their ability to enhance thermal stability of vegetable oils. Determination of the peroxide value (P.V.) and the p-Anisidine value (p-A.V.) was done according to the standard methods of analysis. The compounds were tested for in vitro cytotoxicity against a mammalian cell-line, Chinese Hamster Ovarian (CHO) using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazoliumbromide (MTT)-assay. Studies on changes in peroxide and p-Anisidine values for the oils heated to temperatures between 180°C and 200°C recorded better stability enhancement at 100 ppm concentration with these flavonoids than the commercial antioxidant, butylated hydroxytoluene (BHT). The plant-based flavonoids had no significant cytotoxic effect against the CHO cell-line and may serve as alternative antioxidants to synthetic ones which have previously raised great concern over the health of consumers.
