The present study investigated the effects of Lactobacillus acidophilus(LBA)and mannan-oligosaccharides(MOS)supplementation on the production performance,serum biochemistry,antioxidant profile,health indices,meat qual...The present study investigated the effects of Lactobacillus acidophilus(LBA)and mannan-oligosaccharides(MOS)supplementation on the production performance,serum biochemistry,antioxidant profile,health indices,meat quality,and lipid oxidative stability of broiler chicken.A total of 252 commercial broiler chickens at 1 d old of uniform body weight were randomly allocated to 6 maize-soybean-based dietary treatments:T1(control diet),T2(antibiotic bacitracin methylene di-salicylate[BMD]at 20 mg/kg diet),T3(MOS at 0.1%+LBA at 10^6 CFU/g feed),T4(MOS at 0.1%+LBA at 10^7 CFU/g feed),T5(MOS at 0.2%+LBA at10^6 CFU/g feed),and T6(MOS at 0.2%+LBA at 10^7 CFU/g feed).Each treatment was assigned to 6 replicates of 7 birds.The samples for meat quality and serum biochemistry analysis were taken from 12 birds per treatment(2 birds/replicate).The results revealed better(P<0.01)growth performance and production efficiency of birds fed either T5 or T6 diet compared to control or BMD supplemented diet and BMD-supplemented birds superseded the control birds.Higher(P<0.01)serum and liver antioxidant enzyme activities,meat antioxidant capacity(2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid[ABTS]and 1,1-diphenyl-2-picrylhydrazyl[DPPH]assays),serum total protein,high-density lipoproteins(HDL)cholesterol(P<0.05),and globulin levels(P<0.01)were observed in birds fed either T5 or T6 diet compared to control or BMD supplemented birds,whereas,lower lipid oxidation(P<0.01),cardiac risk ratio,atherogenic coefficient,atherogenic index of plasma,serum glucose,triglyceride,total cholesterol levels(P<0.01),and serum albumin-to-globulin ratio(P<0.05)were observed in the chickens.The pH of meat from birds fed T4,T5 or T6 diet was lower(P<0.01)compared to control and other treatments.The extract release volume(ERV),water holding capacity(WHC),and protein content of meat were higher(P<0.05)in birds fed either T5 or T6 diet compared to control or BMD supplemented birds.Thus,it was concluded that the supplementation of 0.2%MOS along with LBA at 10^6 CFU/g is optimum for better growth performance,serum biochemistry,antioxidant profile,health indices,meat quality,and lipid oxidative stability of broiler chickens.展开更多
The present study was to evaluate thyme and ajwain as antibiotic growth promoter substitutions on growth performance,carcass characteristics and serum biochemistry in Japanese quails(Coturnix japonica).A feeding trial...The present study was to evaluate thyme and ajwain as antibiotic growth promoter substitutions on growth performance,carcass characteristics and serum biochemistry in Japanese quails(Coturnix japonica).A feeding trial was conducted over 28 d with 4 groups of Japanese quails fed experimental diets formulated to provide no supplementation(control),or control t 55 mg zinc bacitracin/kg,2 g thyme/kg,and 2 g ajwain/kg.At 35 d of age,2 quails from each replicate were sacrificed and eviscerated.Liver,empty small intestine and heart were weighed and calculated as a percentage of live body weight.The carcasses were weighed and the cuts were performed to evaluate the yield of legs and breast.At 35 d of age,2 quails per replicate were chosen and approximately 1-m L blood samples were collected through brachial vein,and concentrations of albumin,total protein,triglyceride,total cholesterol,and high density lipoprotein(HDL)-cholesterol were determined.Final body weight were not affected by the dietary treatment whereas,it tended to increase in quails supplemented with antibiotic.Overall feed conversation ratio values were similar though it tended to improve in quails supplemented with thyme compared with other groups.Daily feed intake,internal organ weights and carcass traits were not influenced by the dietary treatments.Serum total cholesterol contents were lower(P < 0.05) in quails supplemented with ajwain compared with control and quails supplemented with antibiotic and thyme.The highest serum HDL-cholesterol was seen in the group supplemented with thyme compared with other groups.It was concluded that the addition of 2 g/kg thyme can improve serum biochemistry in quails,although its effects on performance criteria was negligible.展开更多
[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1&...[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1"laying hens(66 weeks of age)in late period of laying were randomly divided into four groups,six replicates each group,and each group had 27 individuals of laying hens.The laying hens in control group were fed with basal diet,and those in experimental groupsⅠ,Ⅱ and Ⅲ were fed with the basal diets added with 3.33×10^6,1×10^7,3.33×10^7 CFU/g B.coagulans,respectively.The pretrial lasted one week,and the formal test lasted eight weeks.[Result]Compared with the control group,the laying rate in group Ⅰ increased significantly,and the feed-gain ratio in experimental group Ⅱ decreased noticeably while the spleen index increased remarkably;the alkaline phosphatase activities and blood calcium content in experimental groups Ⅰ,Ⅱ and Ⅲ increased significantly(P<0.05),while the triglyceride content decreased remarkably(P<0.05);the urea content in experimental groups Ⅰ and Ⅱ decreased obviously(P<0.05).Adding B.coagulans significantly increased the specific activity of amylase,lipase and protease in various intestinal mucosa of small intestine(P<0.05);adding B.coagulans significantly increased the content of follicle-stimulating hormone(FSH)and estradiol(E2),and significantly increased the mRNA expression of follicle stimulating hormone receptor gene(FSHR).[Conclusion]B.coagulans preparation could significantly improve the production performance of laying hens in late period of laying;appropriately enhance the immune capacity of laying hens;improve the serum biochemical indicators;increase the activity of digestive enzymes in the small intestine;and promote the release of gonadal hormone and the mRNA expression of FSHR gene in the ovary.展开更多
Objective:To assess the potential toxicity of hydroalcoholic extract of Allium affine(A.affine)aerial parts after acute and sub-acute administration in female and male Wistar rats.Methods:For acute toxicity assay,anim...Objective:To assess the potential toxicity of hydroalcoholic extract of Allium affine(A.affine)aerial parts after acute and sub-acute administration in female and male Wistar rats.Methods:For acute toxicity assay,animals orally received the limit test dose of 2000 mg/kg of A.affine extract and were observed for 2 weeks.For sub-acute toxicity study,rats were orally treated with 125,250,and 500 mg/kg/day of the extract over 28 days,and hematological,biochemical,and histological evaluations were then conducted.Results:All rats were alive with normal body weight gain over 14 days,with LD50˃2000 mg/kg.No abnormality in body weight changes but significant increases in the relative weight of spleen and lung were detected after administration of the highest dose of extract for 28 days in sub-acute assay.Hematological analysis showed prominent elevations in total white blood cells in male rats and neutrophils count in female rats after exposure to 500 mg/kg of A.affine extract.In biochemical evaluations,significant increases in serum creatinine level(female rats,250 and 500 mg/kg)and in aspartate aminotransferase(male and female rate,500 mg/kg)and alanine aminotransferase(male,250 and 500 mg/kg and female,500 mg/kg)activities,however,notable decreases in serum blood glucose(male rats,125 and 500 mg/kg),triglycerides(male rats,500 mg/kg and female rates,250 mg/kg),and low-density lipoprotein cholesterol levels(male,250 mg/kg)were found.Histological examinations presented slight portal inflammation in liver tissue,moderate pneumocyte hyperplasia,congestion and peri-bronchial inflammation in lung tissue,and mild histiocytosis and lymphoid follicular activation in spleen tissue after exposure to 500 mg/kg of A.affine extract in male and female animals.Conclusions:The present investigation reveals the safety of A.affine extract at doses of lower than 250 mg/kg in rats and monitoring of lung,spleen,and liver functions is suggested during excessive and prolonged uses.展开更多
基金funded by the Department of Biotechnology,Ministry of Science and Technology,Government of India with Grant No.BT/PR9724/AAQ/1/571/2013
文摘The present study investigated the effects of Lactobacillus acidophilus(LBA)and mannan-oligosaccharides(MOS)supplementation on the production performance,serum biochemistry,antioxidant profile,health indices,meat quality,and lipid oxidative stability of broiler chicken.A total of 252 commercial broiler chickens at 1 d old of uniform body weight were randomly allocated to 6 maize-soybean-based dietary treatments:T1(control diet),T2(antibiotic bacitracin methylene di-salicylate[BMD]at 20 mg/kg diet),T3(MOS at 0.1%+LBA at 10^6 CFU/g feed),T4(MOS at 0.1%+LBA at 10^7 CFU/g feed),T5(MOS at 0.2%+LBA at10^6 CFU/g feed),and T6(MOS at 0.2%+LBA at 10^7 CFU/g feed).Each treatment was assigned to 6 replicates of 7 birds.The samples for meat quality and serum biochemistry analysis were taken from 12 birds per treatment(2 birds/replicate).The results revealed better(P<0.01)growth performance and production efficiency of birds fed either T5 or T6 diet compared to control or BMD supplemented diet and BMD-supplemented birds superseded the control birds.Higher(P<0.01)serum and liver antioxidant enzyme activities,meat antioxidant capacity(2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid[ABTS]and 1,1-diphenyl-2-picrylhydrazyl[DPPH]assays),serum total protein,high-density lipoproteins(HDL)cholesterol(P<0.05),and globulin levels(P<0.01)were observed in birds fed either T5 or T6 diet compared to control or BMD supplemented birds,whereas,lower lipid oxidation(P<0.01),cardiac risk ratio,atherogenic coefficient,atherogenic index of plasma,serum glucose,triglyceride,total cholesterol levels(P<0.01),and serum albumin-to-globulin ratio(P<0.05)were observed in the chickens.The pH of meat from birds fed T4,T5 or T6 diet was lower(P<0.01)compared to control and other treatments.The extract release volume(ERV),water holding capacity(WHC),and protein content of meat were higher(P<0.05)in birds fed either T5 or T6 diet compared to control or BMD supplemented birds.Thus,it was concluded that the supplementation of 0.2%MOS along with LBA at 10^6 CFU/g is optimum for better growth performance,serum biochemistry,antioxidant profile,health indices,meat quality,and lipid oxidative stability of broiler chickens.
基金supported by Islamic Azad University,Shahrekord Branch (grant number:2014/04)
文摘The present study was to evaluate thyme and ajwain as antibiotic growth promoter substitutions on growth performance,carcass characteristics and serum biochemistry in Japanese quails(Coturnix japonica).A feeding trial was conducted over 28 d with 4 groups of Japanese quails fed experimental diets formulated to provide no supplementation(control),or control t 55 mg zinc bacitracin/kg,2 g thyme/kg,and 2 g ajwain/kg.At 35 d of age,2 quails from each replicate were sacrificed and eviscerated.Liver,empty small intestine and heart were weighed and calculated as a percentage of live body weight.The carcasses were weighed and the cuts were performed to evaluate the yield of legs and breast.At 35 d of age,2 quails per replicate were chosen and approximately 1-m L blood samples were collected through brachial vein,and concentrations of albumin,total protein,triglyceride,total cholesterol,and high density lipoprotein(HDL)-cholesterol were determined.Final body weight were not affected by the dietary treatment whereas,it tended to increase in quails supplemented with antibiotic.Overall feed conversation ratio values were similar though it tended to improve in quails supplemented with thyme compared with other groups.Daily feed intake,internal organ weights and carcass traits were not influenced by the dietary treatments.Serum total cholesterol contents were lower(P < 0.05) in quails supplemented with ajwain compared with control and quails supplemented with antibiotic and thyme.The highest serum HDL-cholesterol was seen in the group supplemented with thyme compared with other groups.It was concluded that the addition of 2 g/kg thyme can improve serum biochemistry in quails,although its effects on performance criteria was negligible.
文摘[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1"laying hens(66 weeks of age)in late period of laying were randomly divided into four groups,six replicates each group,and each group had 27 individuals of laying hens.The laying hens in control group were fed with basal diet,and those in experimental groupsⅠ,Ⅱ and Ⅲ were fed with the basal diets added with 3.33×10^6,1×10^7,3.33×10^7 CFU/g B.coagulans,respectively.The pretrial lasted one week,and the formal test lasted eight weeks.[Result]Compared with the control group,the laying rate in group Ⅰ increased significantly,and the feed-gain ratio in experimental group Ⅱ decreased noticeably while the spleen index increased remarkably;the alkaline phosphatase activities and blood calcium content in experimental groups Ⅰ,Ⅱ and Ⅲ increased significantly(P<0.05),while the triglyceride content decreased remarkably(P<0.05);the urea content in experimental groups Ⅰ and Ⅱ decreased obviously(P<0.05).Adding B.coagulans significantly increased the specific activity of amylase,lipase and protease in various intestinal mucosa of small intestine(P<0.05);adding B.coagulans significantly increased the content of follicle-stimulating hormone(FSH)and estradiol(E2),and significantly increased the mRNA expression of follicle stimulating hormone receptor gene(FSHR).[Conclusion]B.coagulans preparation could significantly improve the production performance of laying hens in late period of laying;appropriately enhance the immune capacity of laying hens;improve the serum biochemical indicators;increase the activity of digestive enzymes in the small intestine;and promote the release of gonadal hormone and the mRNA expression of FSHR gene in the ovary.
基金financially supported by Vice-Chancellery for Research and Technology,Isfahan University of Medical Sciences(Grant No.399187).
文摘Objective:To assess the potential toxicity of hydroalcoholic extract of Allium affine(A.affine)aerial parts after acute and sub-acute administration in female and male Wistar rats.Methods:For acute toxicity assay,animals orally received the limit test dose of 2000 mg/kg of A.affine extract and were observed for 2 weeks.For sub-acute toxicity study,rats were orally treated with 125,250,and 500 mg/kg/day of the extract over 28 days,and hematological,biochemical,and histological evaluations were then conducted.Results:All rats were alive with normal body weight gain over 14 days,with LD50˃2000 mg/kg.No abnormality in body weight changes but significant increases in the relative weight of spleen and lung were detected after administration of the highest dose of extract for 28 days in sub-acute assay.Hematological analysis showed prominent elevations in total white blood cells in male rats and neutrophils count in female rats after exposure to 500 mg/kg of A.affine extract.In biochemical evaluations,significant increases in serum creatinine level(female rats,250 and 500 mg/kg)and in aspartate aminotransferase(male and female rate,500 mg/kg)and alanine aminotransferase(male,250 and 500 mg/kg and female,500 mg/kg)activities,however,notable decreases in serum blood glucose(male rats,125 and 500 mg/kg),triglycerides(male rats,500 mg/kg and female rates,250 mg/kg),and low-density lipoprotein cholesterol levels(male,250 mg/kg)were found.Histological examinations presented slight portal inflammation in liver tissue,moderate pneumocyte hyperplasia,congestion and peri-bronchial inflammation in lung tissue,and mild histiocytosis and lymphoid follicular activation in spleen tissue after exposure to 500 mg/kg of A.affine extract in male and female animals.Conclusions:The present investigation reveals the safety of A.affine extract at doses of lower than 250 mg/kg in rats and monitoring of lung,spleen,and liver functions is suggested during excessive and prolonged uses.
