Objective: To explore the mechanism in patients with irritable bowel syndrome (IBS) of the constipation predominant type and observe the therapeutic effects of Sinisan (四逆散, SNS). Methods: Forty -seven IBS patien...Objective: To explore the mechanism in patients with irritable bowel syndrome (IBS) of the constipation predominant type and observe the therapeutic effects of Sinisan (四逆散, SNS). Methods: Forty -seven IBS patients with the constipation predominant type were randomly divided into the treated group ( n =24) and the control group ( n =23). Another group of 22 healthy subjects was set up for healthy control. The treated group was treated with modified SNS, and the control group was treated with Cisapride, the therapeutic course for both groups was 8 weeks. The changes of symptom scoring and anorectal manometry (the anorectal resting pressure, anal tract systolic pressure, anal tract diastolic pressure, rectal threshold feeling, maximal tolerance volume of rectum, and rectum compliance) of these two groups were recorded respectively and compared with each other. Results: Compared with the healthy control group, the rectal threshold feeling, maximal tolerance volume of rectum and rectal compliance of the treated groups got reduced significantly before treatment ( P <0.05). After treatment, the symptom scoring, rectal threshold feeling and maximal tolerance volume of rectum were improved in both groups ( P <0.05), and the improvement of the treated group was more significant than that of the control group( P <0.01). The total effective rate and recurrence rate of the treated group were superior to those of the control group significantly ( P <0.05, P <0.01).Conclusion: SNS has good effect on IBS of the constipation predominant type.展开更多
Aim To study the influence of SiniSan on the Tryptophan- kynurenine（TRP-KYN）pathway, the activity and content of the key metabolism enzyme indoleamine2, 3-dioxygenase （IDO）. Methods The mice model was established ...Aim To study the influence of SiniSan on the Tryptophan- kynurenine（TRP-KYN）pathway, the activity and content of the key metabolism enzyme indoleamine2, 3-dioxygenase （IDO）. Methods The mice model was established by intraperitoneal injection LPS （ 1 mg · kg^-1 ）. Using ELISA to detect the contents of IFN-γin serum. The content of Tryptophan and kynurenine in brain tissue were detected by using HPLC-MS/MS technique. The mRNA expression level of IDO in brain tissue was detected by using real-time PCR. Results 4 hours after the LPS injection, the immobility time of model group mice prolonged. The content of IFN-~/in serum increased significantly （P 〈 0.05）. Meanwhile, the activity of IDO in brain tissue and the mRNA expression level of IDO increased. SiniSan could short the immobility time of mice, reduced the content of IFN-γin serum （P 〈 0.05）. It could also inhibit the activity of IDO and the expression of mRNA（P 〈 0.01 ）. Conclusion SiniSan blocks the pathway of IDO activation, inhibit the activity of IDO and reduce the content of it. SiniSan produces antidepressant effect by adjusting the TRP-KYN metabolism.展开更多
文摘Objective: To explore the mechanism in patients with irritable bowel syndrome (IBS) of the constipation predominant type and observe the therapeutic effects of Sinisan (四逆散, SNS). Methods: Forty -seven IBS patients with the constipation predominant type were randomly divided into the treated group ( n =24) and the control group ( n =23). Another group of 22 healthy subjects was set up for healthy control. The treated group was treated with modified SNS, and the control group was treated with Cisapride, the therapeutic course for both groups was 8 weeks. The changes of symptom scoring and anorectal manometry (the anorectal resting pressure, anal tract systolic pressure, anal tract diastolic pressure, rectal threshold feeling, maximal tolerance volume of rectum, and rectum compliance) of these two groups were recorded respectively and compared with each other. Results: Compared with the healthy control group, the rectal threshold feeling, maximal tolerance volume of rectum and rectal compliance of the treated groups got reduced significantly before treatment ( P <0.05). After treatment, the symptom scoring, rectal threshold feeling and maximal tolerance volume of rectum were improved in both groups ( P <0.05), and the improvement of the treated group was more significant than that of the control group( P <0.01). The total effective rate and recurrence rate of the treated group were superior to those of the control group significantly ( P <0.05, P <0.01).Conclusion: SNS has good effect on IBS of the constipation predominant type.
文摘Aim To study the influence of SiniSan on the Tryptophan- kynurenine（TRP-KYN）pathway, the activity and content of the key metabolism enzyme indoleamine2, 3-dioxygenase （IDO）. Methods The mice model was established by intraperitoneal injection LPS （ 1 mg · kg^-1 ）. Using ELISA to detect the contents of IFN-γin serum. The content of Tryptophan and kynurenine in brain tissue were detected by using HPLC-MS/MS technique. The mRNA expression level of IDO in brain tissue was detected by using real-time PCR. Results 4 hours after the LPS injection, the immobility time of model group mice prolonged. The content of IFN-~/in serum increased significantly （P 〈 0.05）. Meanwhile, the activity of IDO in brain tissue and the mRNA expression level of IDO increased. SiniSan could short the immobility time of mice, reduced the content of IFN-γin serum （P 〈 0.05）. It could also inhibit the activity of IDO and the expression of mRNA（P 〈 0.01 ）. Conclusion SiniSan blocks the pathway of IDO activation, inhibit the activity of IDO and reduce the content of it. SiniSan produces antidepressant effect by adjusting the TRP-KYN metabolism.
