The discharge of the antibiotic wastewater has increased dramatically in our country with the development of medical science and wide application of antibiotic,resulting in serious harm to human body and ecological en...The discharge of the antibiotic wastewater has increased dramatically in our country with the development of medical science and wide application of antibiotic,resulting in serious harm to human body and ecological environment.In this work,ciprofloxacin(CIP)was selected as one of typical antibiotics and heterogeneous Fenton-like catalysts were prepared for the treatment of ciprofloxacin wastewater.The sodium alginate(SA)gel microspheres catalysts were prepared by polymerization method using double metal ions of Fe^(3+)and Mn^(2+)as cross-linking agents.Preparation conditions such as metal ions concentration,mass fraction of SA,polymerization temperature and dual-metal ions as crosslinking agent were optimized.Moreover,the effects of operating conditions such as initial concentration of CIP,pH value and catalyst dosage on CIP removal were studied.The kinetic equation showed that the effect of the initial concentration of CIP on the degradation rate was in line with second-order kinetics,and the effects of catalyst dosage and pH value on the degradation rate of CIP were in line with first-order kinetics.The SA gel microspheres catalysts prepared by dual-metal ions exhibited a high CIP removal and showed a good reusability after six recycles.The SA gel microspheres catalysts with an easy recovery performance provided an economical and efficient method for the removal of antibiotics in the future.展开更多
In the study, we report the comparative efficacy of the sodium hyaluronate gel vaginal application and promestriene cream which were tested to treat the gynecological symptoms of people who suffered from vaginal dryne...In the study, we report the comparative efficacy of the sodium hyaluronate gel vaginal application and promestriene cream which were tested to treat the gynecological symptoms of people who suffered from vaginal dryness. Method: 35 patients applied sodium hyaluronate vaginal gel one time a day for a period of two times a week during three weeks and other 33 patients using promestriene cream applied it every day for a period of three weeks also. Results: No significant difference was observed between vaginal application of sodium hyaluronate gel and promestriene cream, regarding both, as regarding the dryness of the intimate mucosa (p = 0.786), the attribute of moisturizing properties to the intimate mucosa (p = 0.142), the comfort sensation (p = 0.528), and no significant difference was observed regarding the fragrance of the product (p = 0.088). Conclusion: The similar results between vaginal application of both products support the use of sodium hyaluronate vaginal gel (Lubrinat®) in the initial approach of symptoms of vaginal dryness.展开更多
AIM:To evaluate the efficacy and safety of sodium hyaluronate solution(SH) in endoscopic submucosal dissection(ESD) of gastric neoplasms.METHODS:A prospective multicenter randomized,double blind,controlled trial was d...AIM:To evaluate the efficacy and safety of sodium hyaluronate solution(SH) in endoscopic submucosal dissection(ESD) of gastric neoplasms.METHODS:A prospective multicenter randomized,double blind,controlled trial was designed and utilized in this study.A total of 76 patients with 5-20 mm sized gastric neoplasms were enrolled at three academic hospitals in South Korea from June 2011 to October 2011.Patients were randomly assigned to the 0.4% sodium hyaluronate or control groups.All lesions underwent endoscopic ESD.ESD was performed with 0.4%SH and normal saline(NS) solution for submucosal injection.Efficacy was assessed using en bloc resection and the number of additional injections.Secondary evaluation variables were the volume of injection material,steepness of mucosal elevation,bleeding rate,procedural time and operator satisfaction.Finally,the safety was assessed by analyzing adverse events during the study.RESULTS:The usefulness rate in the 0.4%SH group and the controlled group had statistically significant difference under intention to treat(ITT) analysis(90.91% vs 61.11% P = 0.0041).Under per protocol(PP),the usefulness rate is statistically significant different(93.10% vs 61.76%,P = 0.0036).The difference in volume of the solution injected between 0.4%SH group and the controlled group and NS group was also statistically significant under intention to treat and per protocol analysis(ITT:0.03 ± 0.02 mL vs 0.06 ± 0.03 mL,P = 0.0003,PP:0.03 ± 0.02 mL vs 0.06 ± 0.03 mL,P = 0.0004).Satisfaction above the grade good was significantly higher in the SH group under intention to treat and per protocol analysis(ITT:90.91% vs 61.11%,P = 0.0041,PP = 93.11% vs 61.77%,P = 0.0022).Adverse events above grade 3 were not noticed in either group.All adverse events were treated and were judged as not associated with the submucosal injection solutions.CONCLUSION:0.4%SH solution is a safe and effective agent that doesn't cause any significant adverse events and is useful for submucosal injection during ESD.展开更多
In an acetic acid-sodium acetate buffer solution ofpH 3.6-6.8,a compound complex was formed between sodium hyaluronate (abbreviated as SH)and some basic bisphenylnaphthylmethane dyes,leading to a great enhancement of ...In an acetic acid-sodium acetate buffer solution ofpH 3.6-6.8,a compound complex was formed between sodium hyaluronate (abbreviated as SH)and some basic bisphenylnaphthylmethane dyes,leading to a great enhancement of the intensity of resonance Rayleigh scattering(RRS)and giving a new RRS spectrum,with its maximum scattering peak near 280 um.It was also found that the intensity of RRS was directly proportional to the concentration of SH near the range between 0 and 3.0 mg/L.Based on these facts,a sensitive method for the determination of SH has been established.The method had good selectivity,and has been used for the determination of total amounts of SH in samples with satisfactory results.For the NB-SH system,the detection limit of SH was down to 13.7 ng/mL.展开更多
A liquid chromatography(HPLC)method with UV detection was developed for determination of sodium hyaluronate in pharmaceutical formulation.Sodium hyaluronate is a polymer of disaccharides,composed of D-glucuronic acid ...A liquid chromatography(HPLC)method with UV detection was developed for determination of sodium hyaluronate in pharmaceutical formulation.Sodium hyaluronate is a polymer of disaccharides,composed of D-glucuronic acid and D-N-acetylglucosamine,linked via alternatingβ-1,4 andβ-1,3glycosidic bonds.Being a polymer compound it lacks a UV absorbing chromophore.In the absence of a UV absorbing chromophore and highly polar nature of compound,the analysis becomes a major challenge.To overcome these problems a novel method for the determination of sodium hyaluronate was developed and validated based on size exclusion liquid chromatography(SEC)with UV detection.An isocratic mobile phase consisting of buffer 0.05 M potassium dihydrogen phosphate,pH adjusted to 7.0using potassium hydroxide(10%)was used.Chromatography was carried out at 25 1C on a BioSep SEC S2000,300 mm 7.8 mm column.The detection was carried out using variable wavelength UV–vis detector set at 205 nm.The compounds were eluted isocratically at a steady fow rate of 1.0 mL/min.Sodium hyaluronate retention time was about 4.9 min with an asymmetry factor of 1.93.A calibration curve was obtained from 1 to 38 g/mL(r40.9998).Within-day%RSD was 1.0 and between-day%RSD was 1.10.Specifcity/selectivity experiments revealed the absence of interference from excipients,recovery from spiked samples for sodium hyaluronate was 99–102.The developed method was applied to the determination of sodium hyaluronate in pharmaceutical drug substance and product.展开更多
Objective:To identify the in vitro effects of sodium hyaluronate(HA) on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease(KBD) and osteoarthritis(OA). Methods:Samples of articu...Objective:To identify the in vitro effects of sodium hyaluronate(HA) on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease(KBD) and osteoarthritis(OA). Methods:Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers(6 subjects in each of the 3 groups) were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups: H0(no HA), H100(HA, 0.1 g/L) and H500(HA, 0.5 g/L). The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results:HA promoted proliferation of chondrocytes in all the three groups, and in KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 < HA100 < H0. Conclusion:Sodium hyaluronate administration has a dose-dependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.展开更多
AIM: To investigate the effects of different concentrations of artificial tears on lipid layer thickness(LLT) and blink rate(BR) in dry eye patients.METHODS: This study included 106 eyes of 58 patients with dry eye. T...AIM: To investigate the effects of different concentrations of artificial tears on lipid layer thickness(LLT) and blink rate(BR) in dry eye patients.METHODS: This study included 106 eyes of 58 patients with dry eye. The lipid deficiency type was defined as the LLT baseline <75 nm. The LLT and BR were measured at baseline and 1, 5 and 15 min after the instillation of 0.1% or 0.3% sodium hyaluronate(SH) eye drops by using the LipiV iew ocular surface interferometer. RESULTS: In the lipid deficiency group, the LLT increased from baseline at 1 min post instillation. The LLT after the instillation of 0.1% SH was significantly higher than that after the instillation of 0.3% SH(P<0.001). The LLT returned to baseline at 15 min post instillation of 0.1% SH and at 5 min post instillation of 0.3% SH. In the non-lipid deficiency group, the LLT decreased from baseline at 1 min and returned to baseline at 5 min for both treatments. The BRs were not significantly different at different time points for both treatments.CONCLUSION: SH eye drops induce a short-term increase in LLT of patients with lipid deficiency. A low concentration of artificial tears have a stronger effect than a high concentration of artificial tears on the increase in LLT. In comparison, SH eye drops induce a transient and slight decrease in LLT of patients without lipid deficiency. A low concentration of artificial tears might be better for patients with lipid deficiency.展开更多
Objective: To investigate the effect of arthroscopic debridement combined with intra-articular sodium hyaluronate injection on the Wnt/β-catenin pathway in joint fluid of patients with knee osteoarthritis. Methods: P...Objective: To investigate the effect of arthroscopic debridement combined with intra-articular sodium hyaluronate injection on the Wnt/β-catenin pathway in joint fluid of patients with knee osteoarthritis. Methods: Patients undergoing arthroscopic debridement for knee osteoarthritis in the hospital between June 2014 and April 2017 were selected as the research subjects and randomly divided into the observation group who accepted arthroscopic debridement combined with intra-articular sodium hyaluronate injection and the control group who accepted arthroscopic debridement alone. The Wnt/β-catenin pathway molecules as well as downstream collagen metabolism molecules and bone metabolism molecules in the joint fluid were measured before surgery and 7 d after surgery. Results: 7 d after surgery, Wnt1, Wnt5a, Wnt7b, β-catenin, MMP1, MMP13, CTX-I, CTX-II, ADAMTS4, ADAMTS5, RANK, RANKL and TRACP5b mRNA expression in joint fluid of both groups of patients were higher than those before surgery whereas OPG and OC contents were lower than those before surgery, and Wnt1, Wnt5a, Wnt7b, β-catenin, MMP1, MMP13, CTX-I, CTX-II, ADAMTS4, ADAMTS5, RANK, RANKL and TRACP5b mRNA expression in joint fluid of observation group were significantly lower than those of control group whereas OPG and OC contents were significantly higher than those of control group. Conclusion: Arthroscopic debridement combined with intra-articular sodium hyaluronate injection can inhibit the collagen metabolism and bone metabolism disorder mediated by Wnt/β-catenin after surgery.展开更多
Objective: To study the clinical effect of rehabmtation treating combined with intra-articular injection of sodium hyaluronate for exercise induced knee osteoarthritis, and to research the function, biomechanics of kn...Objective: To study the clinical effect of rehabmtation treating combined with intra-articular injection of sodium hyaluronate for exercise induced knee osteoarthritis, and to research the function, biomechanics of knee joint, and inflammatory factor in articular cavity. Method:Selecting 120 patients with exercise induced knee osteoarthritis, then they were randomly divided into control group (n=60) and intervene group (n=60). The patients in the control group were given conventional rehabmtation treating, and the intervene group were given intra-articular injection of sodium hyaluronate on this basic, they were treated 4 weeks. Evaluating the function of knee joint;conduct isokinetic muscle strength test (determination of the angIllar velocity were 60°/S, 180°/S);test the ratio of muscle peak torque and four muscles centripetal centripetal peak torque and peak torque, work load of femoral knee joints after (simultaneous determination of the angular velocity were 60°/S);detect the inflammatory factor in the joint fluid. Results: After the treatment, clinical effect in intervene group was superior to that in control group,but had no statistical significance;after the treatment, the score of function of knee joint in the two groups were higher than before treatment, and had statistical significance, and the score of function of knee joint in the intervene group were higher than control group, and the difference had statistical significance;Angular velocity of 60°/S and 180°/S the two groups peak torque, work load and the H/Q values were significantly increased, the intervene group were higher than control group, and the difference had statistical significance;inflammatory factor in the two groups were significantly decreased, the intervene group were lower than control group, and the difference had statistical significance.Conclusions: On the basic of rehabmtation treating, the treatment of intra-articular injection of sodium hyaluronate can improve the function of knee joint through improving the knee muscle strength, the level of their participation in the activities of the biomechanics of knee joint, and the inflammatory factor in the joint fluid.展开更多
AIM: To investigate the anti-scarring effect of sodium hyaluronate(HA) at filtration pathway after filtering surgery in a rabbit model.METHODS: Fifteen healthy adult New Zealand white rabbits were selected for trabecu...AIM: To investigate the anti-scarring effect of sodium hyaluronate(HA) at filtration pathway after filtering surgery in a rabbit model.METHODS: Fifteen healthy adult New Zealand white rabbits were selected for trabeculectomy in both eyes. The right eyes were used as HA group with 0.1 m L HA injected into the anterior chamber at the end of the operation;the left eyes were used with 0.1 m L sodium lactate Ringer’s solution(RS) injected into the anterior chamber as RS group. Intraocular pressure(IOP), filtering blebs morphology, inflammatory reaction and complications were observed at the 7, 60, and 90 d after surgery.RESULTS: One day after surgery, the IOP of HA and RS groups were 12.75±1.92 and 10.50±1.59 mm Hg(P=0.005). At the 7;day postoperative, the filtering blebs of each group were functional type and TGF-β expression was significantly difference in both groups(0.10±0.01 vs 0.14±0.02, P=0.024). After 60 d of the operation, all filtering blebs were scarring and alpha-smooth muscle actin(α-SMA) expression was significantly difference in both groups(0.40±0.04 vs 0.35±0.02, P=0.032). α-SMA positive cells were mainly distributed in the junction of conjunctiva and sclera and around the blood vessels. The collagen volume fraction(CVF) of HA and RS group was(75.49±7.01)% and(79.93±5.35)%(P=0.044). On the 90;day after the operation, CVF was(82.57±5.19)% and(88.08±1.75)% in HA and RS groups(P=0.036). There was no α-SMA positive cell in HA group, while a few positive cells were observed in RS group(P=0.000).CONCLUSION: HA has effect of anti-scar and antiinflammation on filtration pathway after filtering surgery within 3 mo by inhibiting fibroblast proliferation and collagen deposition.展开更多
A transparent bulk sodium borosilicate gel with the composition of 72SiO2·23B2O3·5Na2O (wt pct) was prepared by using tetraethyl orthosilicate, boric acid, sodium methoxide, sodium alkoxide, sodium acetate a...A transparent bulk sodium borosilicate gel with the composition of 72SiO2·23B2O3·5Na2O (wt pct) was prepared by using tetraethyl orthosilicate, boric acid, sodium methoxide, sodium alkoxide, sodium acetate as precursor, methanol, ethanol and glycolic methyl ether as precursor solvent and hydrochloric acid as catalyst. Properties of gel, varied principle and related mechanism of conversion for sol to gel are discussed. Ideal composition and dry process are given in this展开更多
Posterior disc displacement of the temporomandibular joint is rare. We present a unique treatment method and magnetic resonance (MR) images of this condition. The patient was a 43-year-old male with a chief complaint ...Posterior disc displacement of the temporomandibular joint is rare. We present a unique treatment method and magnetic resonance (MR) images of this condition. The patient was a 43-year-old male with a chief complaint of abnormal occlusion. Regarding the present status, there was no occlusion on the right side. No temporomandibular joint pain, myalgia, or clicking were observed while mouth opening. On MR images, the posterior disc displacement without reduction on the right was observed and it was assumed that occlusal abnormality was due to this condition. We treated him with the following methods. After injection into the superior articular cavity with 2% lidocaine, a sodium hyaluronate preparation was injected followed by intermaxillary traction applied using rubber. The recovery of occlusion was confirmed in follow-up at 5 days after treatment. In the 13th days, the intermaxillary traction was removed. On MR images, the right disc condition was changed to anterior disc displacement with reduction. We consider our treatment methods are effective for this clinical condition.展开更多
Objective:To explore the meta-analysis of traditional Chinese medicine fumigation combined with sodium hyaluronate in the treatment of knee osteoarthritis.Methods: The database was originated from CNKI, Wanfang, Chong...Objective:To explore the meta-analysis of traditional Chinese medicine fumigation combined with sodium hyaluronate in the treatment of knee osteoarthritis.Methods: The database was originated from CNKI, Wanfang, Chongqing Weipu database, PubMed and so on. The deadline was April 2017. The database was searched with the keywords of'Chinese herbal fumigation','sodium hyaluronate' and'knee osteoarthritis'. Finally, 13 articles about the treatment of Chinese herbal fumigation combined with sodium hyaluronate were obtained. RCT literatures of knee osteoarthritis identified the outcome indicators as the effective rate and recovery rate. Lysholm's score was observed after treatment in both groups, and all the results were input into Review Manager 5.2 for analysis.Result: A total of 13 RCT literatures about the treatment of knee osteoarthritis with Chinese herbal fumigation and sodium hyaluronate were obtained. The control group was treated with sodium hyaluronate or anti-inflammatory drugs, totaling 619 cases. The study group was treated with sodium hyaluronate and Chinese herbal fumigation, totaling 649 cases. The combined effect of forest map OR=1.82, Z=5.00 (P<0.00001), 95% CI. (1.44, 2.30). Compared with sodium hyaluronate or anti-inflammatory drugs, fumigation with traditional Chinese medicine combined with sodium hyaluronate has obvious advantages in curing knee osteoarthritis. The combined effect of forest chart OR=5.55, Z=7.44 (P<0.00001), 95% CI (3.54, 8.73), indicating that compared with sodium hyaluronate or anti-inflammatory drugs, traditional Chinese medicine is used. Fumigation combined with sodium hyaluronate has obvious advantages in the treatment of knee osteoarthritis;forest map combined effect OR=12.11, Z=5.18 (P<0.00001), 95% CI (7.53, 16.68), indicating that compared with sodium hyaluronate or anti-inflammatory drugs, traditional Chinese medicine fumigation combined with sodium hyaluronate in the treatment of knee osteoarthritis can restore knee joint function. Advantages.Conclusion: Chinese herbal fumigation combined with sodium hyaluronate has obvious advantages in curing and effective rate of knee osteoarthritis, and can better restore knee joint function. Due to the lack of literature and low quality, the exact effect still needs to be confirmed by higher quality RCT literature.展开更多
Objective:To research the sodium hyaluronate joint cavity filling combined with exercise therapy on the indicator of pain,motor function,and levels of inflammatory factors in joint fluid in patients with post-traumati...Objective:To research the sodium hyaluronate joint cavity filling combined with exercise therapy on the indicator of pain,motor function,and levels of inflammatory factors in joint fluid in patients with post-traumatic knee arthritis.Method Totally 100 patients(Department of Orthopaedics,The second Affiliated Hospital to Liaoning University of Chinese Medicine,2013.10-2018.8)with post-traumatic knee arthritis were selected,then they were divided into observation group(n=50)and control group(n=50).The control group was given glucosamine sulfate and sodium hyaluronate joint cavity filling,the observation group was given leg press with visual feedback,they were treated 4 weeks.Assessing the pain degree through visual analog scale(VAS),hospital for special surgery knee score(HSS)as the indicator of motor function,inflammatory factor(TNF-α,IL-6,and IL-8)in the joint fluid.Results The score of VAS were lower than pretherapy(P<0.05),the degree of VAS of observation group were lower than control group(P<0.05).The score of HSS were higher than pretherapy(P<0.05),the degree of VAS of observation group were higher than control group(P<0.05).The inflammatory factor(TNF-α,IL-6,and CRP)in the joint fluid were lower than pretherapy(P<0.05),and the observation group were lower than control group(P<0.05).Conclusions Giving sodium hyaluronate joint cavity filling combined with exercise therapy can improve the function of joint.展开更多
Objective To evaluate the clinical effect of sodium hyaluronate product(SHP) used as inhibitor to preventing the formation of epidural scar.Method 160 patients suffering from lumbar intervertebral disc herniation were...Objective To evaluate the clinical effect of sodium hyaluronate product(SHP) used as inhibitor to preventing the formation of epidural scar.Method 160 patients suffering from lumbar intervertebral disc herniation were treated by operation,during which SHP was daubed around dura matter as obstruct to prevent epidural scar formation.Result No adverse reaction was observed.Pain was relieved and VAS was decreassed from 8.35 to 0.42 in mean.Using SHP got better curative effect than control group did.The cure and mend rate is 100%.No relapse occurs.Less scar formation was proved by CT in some cases.Conclusion SHP is ideal material used as inhibitor of epidural cicatrization.展开更多
Bioactive glass ceramic with SiO2-Ca2O-Na2O-P2O5 composition was prepared by the sol-gel method using sodium metasilicate (Na2SiO3) as silica source. The monolith obtained was sintered at 1000?C for 2 hours after whic...Bioactive glass ceramic with SiO2-Ca2O-Na2O-P2O5 composition was prepared by the sol-gel method using sodium metasilicate (Na2SiO3) as silica source. The monolith obtained was sintered at 1000?C for 2 hours after which X-ray diffraction (XRD) analysis showed presence of combeite (Na2Ca2Si3O9) as the crystalline phase. In vitro bioactivity test conducted on the material using simulated body fluid (SBF) showed the formation of carbonated hydroxyapatite on its surface. The material during the SBF test was observed to transform from a mechanically strong crystalline phase Na2Ca2Si3O9 to an amorphous phase after incubation for 14 days indicating that the material was biodegradable. Scanning electron microscopy (SEM) was used to investigate the surface morphology, while Fourier transform infrared (FTIR) spectroscopy facilitated the confirmation of hydroxyapatite (HA) formation. The monolith material obtained may be a good candidate for application in tissue engineering scaffolds.展开更多
基金supported by the National Natural Science Foundation of China(22125802 and 22108012)Natural Science Foundation of Beijing Municipality(2222017)Fundamental Research Funds for the Central Universities(BUCTRC-202109)。
文摘The discharge of the antibiotic wastewater has increased dramatically in our country with the development of medical science and wide application of antibiotic,resulting in serious harm to human body and ecological environment.In this work,ciprofloxacin(CIP)was selected as one of typical antibiotics and heterogeneous Fenton-like catalysts were prepared for the treatment of ciprofloxacin wastewater.The sodium alginate(SA)gel microspheres catalysts were prepared by polymerization method using double metal ions of Fe^(3+)and Mn^(2+)as cross-linking agents.Preparation conditions such as metal ions concentration,mass fraction of SA,polymerization temperature and dual-metal ions as crosslinking agent were optimized.Moreover,the effects of operating conditions such as initial concentration of CIP,pH value and catalyst dosage on CIP removal were studied.The kinetic equation showed that the effect of the initial concentration of CIP on the degradation rate was in line with second-order kinetics,and the effects of catalyst dosage and pH value on the degradation rate of CIP were in line with first-order kinetics.The SA gel microspheres catalysts prepared by dual-metal ions exhibited a high CIP removal and showed a good reusability after six recycles.The SA gel microspheres catalysts with an easy recovery performance provided an economical and efficient method for the removal of antibiotics in the future.
基金This study was supported by the grants from the National Science Foundation of China （No. 81670851）, the Natural Science Foundation of Beijing （No. 7172231 ）, and the Capital Public Health Project ofBeijing （No. Z151100003915143）.
文摘In the study, we report the comparative efficacy of the sodium hyaluronate gel vaginal application and promestriene cream which were tested to treat the gynecological symptoms of people who suffered from vaginal dryness. Method: 35 patients applied sodium hyaluronate vaginal gel one time a day for a period of two times a week during three weeks and other 33 patients using promestriene cream applied it every day for a period of three weeks also. Results: No significant difference was observed between vaginal application of sodium hyaluronate gel and promestriene cream, regarding both, as regarding the dryness of the intimate mucosa (p = 0.786), the attribute of moisturizing properties to the intimate mucosa (p = 0.142), the comfort sensation (p = 0.528), and no significant difference was observed regarding the fragrance of the product (p = 0.088). Conclusion: The similar results between vaginal application of both products support the use of sodium hyaluronate vaginal gel (Lubrinat®) in the initial approach of symptoms of vaginal dryness.
文摘AIM:To evaluate the efficacy and safety of sodium hyaluronate solution(SH) in endoscopic submucosal dissection(ESD) of gastric neoplasms.METHODS:A prospective multicenter randomized,double blind,controlled trial was designed and utilized in this study.A total of 76 patients with 5-20 mm sized gastric neoplasms were enrolled at three academic hospitals in South Korea from June 2011 to October 2011.Patients were randomly assigned to the 0.4% sodium hyaluronate or control groups.All lesions underwent endoscopic ESD.ESD was performed with 0.4%SH and normal saline(NS) solution for submucosal injection.Efficacy was assessed using en bloc resection and the number of additional injections.Secondary evaluation variables were the volume of injection material,steepness of mucosal elevation,bleeding rate,procedural time and operator satisfaction.Finally,the safety was assessed by analyzing adverse events during the study.RESULTS:The usefulness rate in the 0.4%SH group and the controlled group had statistically significant difference under intention to treat(ITT) analysis(90.91% vs 61.11% P = 0.0041).Under per protocol(PP),the usefulness rate is statistically significant different(93.10% vs 61.76%,P = 0.0036).The difference in volume of the solution injected between 0.4%SH group and the controlled group and NS group was also statistically significant under intention to treat and per protocol analysis(ITT:0.03 ± 0.02 mL vs 0.06 ± 0.03 mL,P = 0.0003,PP:0.03 ± 0.02 mL vs 0.06 ± 0.03 mL,P = 0.0004).Satisfaction above the grade good was significantly higher in the SH group under intention to treat and per protocol analysis(ITT:90.91% vs 61.11%,P = 0.0041,PP = 93.11% vs 61.77%,P = 0.0022).Adverse events above grade 3 were not noticed in either group.All adverse events were treated and were judged as not associated with the submucosal injection solutions.CONCLUSION:0.4%SH solution is a safe and effective agent that doesn't cause any significant adverse events and is useful for submucosal injection during ESD.
文摘In an acetic acid-sodium acetate buffer solution ofpH 3.6-6.8,a compound complex was formed between sodium hyaluronate (abbreviated as SH)and some basic bisphenylnaphthylmethane dyes,leading to a great enhancement of the intensity of resonance Rayleigh scattering(RRS)and giving a new RRS spectrum,with its maximum scattering peak near 280 um.It was also found that the intensity of RRS was directly proportional to the concentration of SH near the range between 0 and 3.0 mg/L.Based on these facts,a sensitive method for the determination of SH has been established.The method had good selectivity,and has been used for the determination of total amounts of SH in samples with satisfactory results.For the NB-SH system,the detection limit of SH was down to 13.7 ng/mL.
文摘A liquid chromatography(HPLC)method with UV detection was developed for determination of sodium hyaluronate in pharmaceutical formulation.Sodium hyaluronate is a polymer of disaccharides,composed of D-glucuronic acid and D-N-acetylglucosamine,linked via alternatingβ-1,4 andβ-1,3glycosidic bonds.Being a polymer compound it lacks a UV absorbing chromophore.In the absence of a UV absorbing chromophore and highly polar nature of compound,the analysis becomes a major challenge.To overcome these problems a novel method for the determination of sodium hyaluronate was developed and validated based on size exclusion liquid chromatography(SEC)with UV detection.An isocratic mobile phase consisting of buffer 0.05 M potassium dihydrogen phosphate,pH adjusted to 7.0using potassium hydroxide(10%)was used.Chromatography was carried out at 25 1C on a BioSep SEC S2000,300 mm 7.8 mm column.The detection was carried out using variable wavelength UV–vis detector set at 205 nm.The compounds were eluted isocratically at a steady fow rate of 1.0 mL/min.Sodium hyaluronate retention time was about 4.9 min with an asymmetry factor of 1.93.A calibration curve was obtained from 1 to 38 g/mL(r40.9998).Within-day%RSD was 1.0 and between-day%RSD was 1.10.Specifcity/selectivity experiments revealed the absence of interference from excipients,recovery from spiked samples for sodium hyaluronate was 99–102.The developed method was applied to the determination of sodium hyaluronate in pharmaceutical drug substance and product.
基金supported by the Ministry of Science and Technology(2006 DFA33610)the Natural Scientific Fund of China(30630058)the International Co-operative Fund in Shaanxi(2005KW-13)
文摘Objective:To identify the in vitro effects of sodium hyaluronate(HA) on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease(KBD) and osteoarthritis(OA). Methods:Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers(6 subjects in each of the 3 groups) were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups: H0(no HA), H100(HA, 0.1 g/L) and H500(HA, 0.5 g/L). The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results:HA promoted proliferation of chondrocytes in all the three groups, and in KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 < HA100 < H0. Conclusion:Sodium hyaluronate administration has a dose-dependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.
基金Supported by Provincial Frontier and Key Technology Innovation Special Fund of Guangdong Province(No.2015B020227001)Guangzhou Science and Technology Plan Scientific Research Projects(No.201504010023)Fundamental Research Funds of State Key Laboratory of Ophthalmology of China,and Five Five Cultivation Project of Ophthalmic Center,Sun Yat-sen University
文摘AIM: To investigate the effects of different concentrations of artificial tears on lipid layer thickness(LLT) and blink rate(BR) in dry eye patients.METHODS: This study included 106 eyes of 58 patients with dry eye. The lipid deficiency type was defined as the LLT baseline <75 nm. The LLT and BR were measured at baseline and 1, 5 and 15 min after the instillation of 0.1% or 0.3% sodium hyaluronate(SH) eye drops by using the LipiV iew ocular surface interferometer. RESULTS: In the lipid deficiency group, the LLT increased from baseline at 1 min post instillation. The LLT after the instillation of 0.1% SH was significantly higher than that after the instillation of 0.3% SH(P<0.001). The LLT returned to baseline at 15 min post instillation of 0.1% SH and at 5 min post instillation of 0.3% SH. In the non-lipid deficiency group, the LLT decreased from baseline at 1 min and returned to baseline at 5 min for both treatments. The BRs were not significantly different at different time points for both treatments.CONCLUSION: SH eye drops induce a short-term increase in LLT of patients with lipid deficiency. A low concentration of artificial tears have a stronger effect than a high concentration of artificial tears on the increase in LLT. In comparison, SH eye drops induce a transient and slight decrease in LLT of patients without lipid deficiency. A low concentration of artificial tears might be better for patients with lipid deficiency.
文摘Objective: To investigate the effect of arthroscopic debridement combined with intra-articular sodium hyaluronate injection on the Wnt/β-catenin pathway in joint fluid of patients with knee osteoarthritis. Methods: Patients undergoing arthroscopic debridement for knee osteoarthritis in the hospital between June 2014 and April 2017 were selected as the research subjects and randomly divided into the observation group who accepted arthroscopic debridement combined with intra-articular sodium hyaluronate injection and the control group who accepted arthroscopic debridement alone. The Wnt/β-catenin pathway molecules as well as downstream collagen metabolism molecules and bone metabolism molecules in the joint fluid were measured before surgery and 7 d after surgery. Results: 7 d after surgery, Wnt1, Wnt5a, Wnt7b, β-catenin, MMP1, MMP13, CTX-I, CTX-II, ADAMTS4, ADAMTS5, RANK, RANKL and TRACP5b mRNA expression in joint fluid of both groups of patients were higher than those before surgery whereas OPG and OC contents were lower than those before surgery, and Wnt1, Wnt5a, Wnt7b, β-catenin, MMP1, MMP13, CTX-I, CTX-II, ADAMTS4, ADAMTS5, RANK, RANKL and TRACP5b mRNA expression in joint fluid of observation group were significantly lower than those of control group whereas OPG and OC contents were significantly higher than those of control group. Conclusion: Arthroscopic debridement combined with intra-articular sodium hyaluronate injection can inhibit the collagen metabolism and bone metabolism disorder mediated by Wnt/β-catenin after surgery.
基金National Natural Science Foundation Youth Project:81501857.
文摘Objective: To study the clinical effect of rehabmtation treating combined with intra-articular injection of sodium hyaluronate for exercise induced knee osteoarthritis, and to research the function, biomechanics of knee joint, and inflammatory factor in articular cavity. Method:Selecting 120 patients with exercise induced knee osteoarthritis, then they were randomly divided into control group (n=60) and intervene group (n=60). The patients in the control group were given conventional rehabmtation treating, and the intervene group were given intra-articular injection of sodium hyaluronate on this basic, they were treated 4 weeks. Evaluating the function of knee joint;conduct isokinetic muscle strength test (determination of the angIllar velocity were 60°/S, 180°/S);test the ratio of muscle peak torque and four muscles centripetal centripetal peak torque and peak torque, work load of femoral knee joints after (simultaneous determination of the angular velocity were 60°/S);detect the inflammatory factor in the joint fluid. Results: After the treatment, clinical effect in intervene group was superior to that in control group,but had no statistical significance;after the treatment, the score of function of knee joint in the two groups were higher than before treatment, and had statistical significance, and the score of function of knee joint in the intervene group were higher than control group, and the difference had statistical significance;Angular velocity of 60°/S and 180°/S the two groups peak torque, work load and the H/Q values were significantly increased, the intervene group were higher than control group, and the difference had statistical significance;inflammatory factor in the two groups were significantly decreased, the intervene group were lower than control group, and the difference had statistical significance.Conclusions: On the basic of rehabmtation treating, the treatment of intra-articular injection of sodium hyaluronate can improve the function of knee joint through improving the knee muscle strength, the level of their participation in the activities of the biomechanics of knee joint, and the inflammatory factor in the joint fluid.
基金Supported by National Natural Science Fundation of China(No.81974132No.81770927)+1 种基金National Key R&D Program of China(No.2021YFA1101202)Hunan Provincial Health Commission(No.202207022839)。
文摘AIM: To investigate the anti-scarring effect of sodium hyaluronate(HA) at filtration pathway after filtering surgery in a rabbit model.METHODS: Fifteen healthy adult New Zealand white rabbits were selected for trabeculectomy in both eyes. The right eyes were used as HA group with 0.1 m L HA injected into the anterior chamber at the end of the operation;the left eyes were used with 0.1 m L sodium lactate Ringer’s solution(RS) injected into the anterior chamber as RS group. Intraocular pressure(IOP), filtering blebs morphology, inflammatory reaction and complications were observed at the 7, 60, and 90 d after surgery.RESULTS: One day after surgery, the IOP of HA and RS groups were 12.75±1.92 and 10.50±1.59 mm Hg(P=0.005). At the 7;day postoperative, the filtering blebs of each group were functional type and TGF-β expression was significantly difference in both groups(0.10±0.01 vs 0.14±0.02, P=0.024). After 60 d of the operation, all filtering blebs were scarring and alpha-smooth muscle actin(α-SMA) expression was significantly difference in both groups(0.40±0.04 vs 0.35±0.02, P=0.032). α-SMA positive cells were mainly distributed in the junction of conjunctiva and sclera and around the blood vessels. The collagen volume fraction(CVF) of HA and RS group was(75.49±7.01)% and(79.93±5.35)%(P=0.044). On the 90;day after the operation, CVF was(82.57±5.19)% and(88.08±1.75)% in HA and RS groups(P=0.036). There was no α-SMA positive cell in HA group, while a few positive cells were observed in RS group(P=0.000).CONCLUSION: HA has effect of anti-scar and antiinflammation on filtration pathway after filtering surgery within 3 mo by inhibiting fibroblast proliferation and collagen deposition.
文摘A transparent bulk sodium borosilicate gel with the composition of 72SiO2·23B2O3·5Na2O (wt pct) was prepared by using tetraethyl orthosilicate, boric acid, sodium methoxide, sodium alkoxide, sodium acetate as precursor, methanol, ethanol and glycolic methyl ether as precursor solvent and hydrochloric acid as catalyst. Properties of gel, varied principle and related mechanism of conversion for sol to gel are discussed. Ideal composition and dry process are given in this
文摘Posterior disc displacement of the temporomandibular joint is rare. We present a unique treatment method and magnetic resonance (MR) images of this condition. The patient was a 43-year-old male with a chief complaint of abnormal occlusion. Regarding the present status, there was no occlusion on the right side. No temporomandibular joint pain, myalgia, or clicking were observed while mouth opening. On MR images, the posterior disc displacement without reduction on the right was observed and it was assumed that occlusal abnormality was due to this condition. We treated him with the following methods. After injection into the superior articular cavity with 2% lidocaine, a sodium hyaluronate preparation was injected followed by intermaxillary traction applied using rubber. The recovery of occlusion was confirmed in follow-up at 5 days after treatment. In the 13th days, the intermaxillary traction was removed. On MR images, the right disc condition was changed to anterior disc displacement with reduction. We consider our treatment methods are effective for this clinical condition.
基金Projects Funded by the National Natural Science Foundation of China.Project No:81870364.
文摘Objective:To explore the meta-analysis of traditional Chinese medicine fumigation combined with sodium hyaluronate in the treatment of knee osteoarthritis.Methods: The database was originated from CNKI, Wanfang, Chongqing Weipu database, PubMed and so on. The deadline was April 2017. The database was searched with the keywords of'Chinese herbal fumigation','sodium hyaluronate' and'knee osteoarthritis'. Finally, 13 articles about the treatment of Chinese herbal fumigation combined with sodium hyaluronate were obtained. RCT literatures of knee osteoarthritis identified the outcome indicators as the effective rate and recovery rate. Lysholm's score was observed after treatment in both groups, and all the results were input into Review Manager 5.2 for analysis.Result: A total of 13 RCT literatures about the treatment of knee osteoarthritis with Chinese herbal fumigation and sodium hyaluronate were obtained. The control group was treated with sodium hyaluronate or anti-inflammatory drugs, totaling 619 cases. The study group was treated with sodium hyaluronate and Chinese herbal fumigation, totaling 649 cases. The combined effect of forest map OR=1.82, Z=5.00 (P<0.00001), 95% CI. (1.44, 2.30). Compared with sodium hyaluronate or anti-inflammatory drugs, fumigation with traditional Chinese medicine combined with sodium hyaluronate has obvious advantages in curing knee osteoarthritis. The combined effect of forest chart OR=5.55, Z=7.44 (P<0.00001), 95% CI (3.54, 8.73), indicating that compared with sodium hyaluronate or anti-inflammatory drugs, traditional Chinese medicine is used. Fumigation combined with sodium hyaluronate has obvious advantages in the treatment of knee osteoarthritis;forest map combined effect OR=12.11, Z=5.18 (P<0.00001), 95% CI (7.53, 16.68), indicating that compared with sodium hyaluronate or anti-inflammatory drugs, traditional Chinese medicine fumigation combined with sodium hyaluronate in the treatment of knee osteoarthritis can restore knee joint function. Advantages.Conclusion: Chinese herbal fumigation combined with sodium hyaluronate has obvious advantages in curing and effective rate of knee osteoarthritis, and can better restore knee joint function. Due to the lack of literature and low quality, the exact effect still needs to be confirmed by higher quality RCT literature.
文摘Objective:To research the sodium hyaluronate joint cavity filling combined with exercise therapy on the indicator of pain,motor function,and levels of inflammatory factors in joint fluid in patients with post-traumatic knee arthritis.Method Totally 100 patients(Department of Orthopaedics,The second Affiliated Hospital to Liaoning University of Chinese Medicine,2013.10-2018.8)with post-traumatic knee arthritis were selected,then they were divided into observation group(n=50)and control group(n=50).The control group was given glucosamine sulfate and sodium hyaluronate joint cavity filling,the observation group was given leg press with visual feedback,they were treated 4 weeks.Assessing the pain degree through visual analog scale(VAS),hospital for special surgery knee score(HSS)as the indicator of motor function,inflammatory factor(TNF-α,IL-6,and IL-8)in the joint fluid.Results The score of VAS were lower than pretherapy(P<0.05),the degree of VAS of observation group were lower than control group(P<0.05).The score of HSS were higher than pretherapy(P<0.05),the degree of VAS of observation group were higher than control group(P<0.05).The inflammatory factor(TNF-α,IL-6,and CRP)in the joint fluid were lower than pretherapy(P<0.05),and the observation group were lower than control group(P<0.05).Conclusions Giving sodium hyaluronate joint cavity filling combined with exercise therapy can improve the function of joint.
文摘Objective To evaluate the clinical effect of sodium hyaluronate product(SHP) used as inhibitor to preventing the formation of epidural scar.Method 160 patients suffering from lumbar intervertebral disc herniation were treated by operation,during which SHP was daubed around dura matter as obstruct to prevent epidural scar formation.Result No adverse reaction was observed.Pain was relieved and VAS was decreassed from 8.35 to 0.42 in mean.Using SHP got better curative effect than control group did.The cure and mend rate is 100%.No relapse occurs.Less scar formation was proved by CT in some cases.Conclusion SHP is ideal material used as inhibitor of epidural cicatrization.
文摘Bioactive glass ceramic with SiO2-Ca2O-Na2O-P2O5 composition was prepared by the sol-gel method using sodium metasilicate (Na2SiO3) as silica source. The monolith obtained was sintered at 1000?C for 2 hours after which X-ray diffraction (XRD) analysis showed presence of combeite (Na2Ca2Si3O9) as the crystalline phase. In vitro bioactivity test conducted on the material using simulated body fluid (SBF) showed the formation of carbonated hydroxyapatite on its surface. The material during the SBF test was observed to transform from a mechanically strong crystalline phase Na2Ca2Si3O9 to an amorphous phase after incubation for 14 days indicating that the material was biodegradable. Scanning electron microscopy (SEM) was used to investigate the surface morphology, while Fourier transform infrared (FTIR) spectroscopy facilitated the confirmation of hydroxyapatite (HA) formation. The monolith material obtained may be a good candidate for application in tissue engineering scaffolds.
