Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ...Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.展开更多
Aquaporin (AQP) belongs to a highly conserved group of membrane proteins considered as major intrinsic proteins, which facilitate water transport across biological membranes. The discovery of AQPs in plants has result...Aquaporin (AQP) belongs to a highly conserved group of membrane proteins considered as major intrinsic proteins, which facilitate water transport across biological membranes. The discovery of AQPs in plants has resulted in a paradigm shift in the understanding of plant-water relations, however, the potential relationship between the role of aquaporins in regulating plant water balance and drought tolerance still remains elusive. In this study, the gene encoding potato AQP cDNA, StPIP1 (GenBank accession no. DQ999080), was cloned from the leaf of potato cultivar Gannongshu 2 by reverse transcription-PCR (RT-PCR). Sequence alignment was made by BLASTn in GenBank, the phylogenetic analysis was conducted using PHYLIPWY, the 3D structure was predicted in Swiss-Model server. Subcellular localization of StPIP1 was performed by constructing CaMV35S-StPIP1-GFP and rd29A-StPIP1-GFP fusion proteins and transient expression in onion epidermis. To understand StPIP1 physiological functions in potato under various stress conditions, the StPIP1 gene in a reverse orientation was transformed into tobacco driven by the Cauliflower mosaic virus (CMV) 35S promoter. The expression levels of transgenic and wild-type plants were assessed under various abiotic stress conditions using semi-quantitative RT-PCR, and the morphological and physiological responses of transgenic plants to different stress conditions were investigated. The expression of StPIP1 mRNA decreased in transgenic plants under non-stress and stress conditions, however, the reduction was more severer under drought stress. In both non-stress and stress conditions, StPIP1 was expressed predominantly in root. The morphological and physiological investigation showed no significant differences in growth rate, germination rate, and root fresh weight (FW) between transgenic and wild-type plants when grown under favorable conditions. In contrast, under drought stress, the reduction in StPIP1 expression leads to a delay in seed germination and seedling growth, accelerated seedling wilt, and leaf morphological abnormity. Under "enough" water conditions (i.e., water culture), the aerial parts of anti-sense plants showed no differences. However, for the aerial parts to accumulate the same amount of biomass, transgenic plants needed about 3 times more abundant root system to transport water for plant growth than wild-type plants. Morphological investigation showed that the reduction in StPIP1 expression increased the root system in transgenic plants under drought stress. As a result, the increase of root mass might compensate the reduced cellular water permeability in order to ensure a sufficient water supply for the plant. Results demonstrated that StPIP1 plays an important role for water transportation in potato, especially under drought stress conditions. The reduced expression of StPIP1 decreases the cellular water transport and influences the expression of endogenous AQPs genes and thereby, has impacts on seed germination, seedling growth, and stress responses of potato to drought conditions.展开更多
[Objectives]To explore the planting adaptability of vegetables in Shanghai.[Methods]In this paper,cowpea(Vigna unguiculate(L.)Walp.),cucumber(Cucumis sativus L.),eggplant(Solanum melongena L.)and potato(Solanum tubero...[Objectives]To explore the planting adaptability of vegetables in Shanghai.[Methods]In this paper,cowpea(Vigna unguiculate(L.)Walp.),cucumber(Cucumis sativus L.),eggplant(Solanum melongena L.)and potato(Solanum tuberosum L.)were selected as experimental materials and planted in the open air.The growth status,the occurrence of diseases and insect pests,and the taste evaluation of these four kinds of common vegetables were mainly studied.[Results]The results showed that the four kinds of common vegetables in Shanghai had strong growth,strong adaptability,less pests and diseases,and good taste.[Conclusions]The cowpea,cucumber,eggplant,and potato are suitable for planting in Shanghai.展开更多
将1.4kb Class I patatin基因的5′侧翼区与GUS基因融合,构建了双元表达载体pPATIs(含patatin部分信号顺序)和pPATI(不含patatin部分信号顺序)。pPATI通过基因枪介导在块茎切片中获得了瞬间表达。以上建构物通过农杆菌介导转入了马铃薯...将1.4kb Class I patatin基因的5′侧翼区与GUS基因融合,构建了双元表达载体pPATIs(含patatin部分信号顺序)和pPATI(不含patatin部分信号顺序)。pPATI通过基因枪介导在块茎切片中获得了瞬间表达。以上建构物通过农杆菌介导转入了马铃薯品种Desiree。X-Gluc染色(PATIs不能染色)及PCR结果证实已获得转基因植株。利用离体块茎诱导系统,GUS的表达进一步用荧光进行定量检测,结果显示,PATI-GUS的转基因植株中GUS比活性均以块茎明显高于茎段,达10-20倍。蔗糖浓度的升高,PATI-GUS植株中的GUS比活性无明显变化,与前人报道有不同。此外,光照促进PATI-GUS的表达。展开更多
The production of mini-tubers under soilless cultivation system increased the availability of pathogen-free seed potatoes. The objective of this study was to evaluate the rooting capability of mini-cuttings of potato ...The production of mini-tubers under soilless cultivation system increased the availability of pathogen-free seed potatoes. The objective of this study was to evaluate the rooting capability of mini-cuttings of potato clones as a function of the irrigation solution and the physiological age of the mother plant, under soilless cultivation system. Potato mini-cuttings were collected from young and mature plants of the cultivars Asterix and Macaca and the advanced clone SMINIA 793101-3. The young plants were newly acclimatized individuals and the mature plants were at the stage of producing mini-tubers. In the first experiment, mini-cuttings originating from summer pruning of mature plants were grown under closed soilless cultivation system irrigated with a nu- trient solution developed for the production of potato mini-tubers and a solution comprised of tap water only. The experiment was conducted in a 3 × 2 factorial arrangement (clones and irrigation solutions) in the complete random design with four replicates of 15 mini-cuttings. In the second experiment, the young and mature mini-cuttings were established under a closed soilless cultivation system irrigated with nutrient solution. The experiment was conducted in a 3 × 2 factorial arrangement (clones and physiological age of the mother plant) in the complete random design with four replicates of 15 mini-cuttings. In both experiments, the percentage of rooting and survival of mini-cuttings, number of roots and length of the longest root were assessed at 21 days of cultivation. Potato mini-cuttings can be rooted under closed soilless cultivation system irrigated with nutrient solution or tap water. Mini-cuttings of young plants have higher rooting capability compared to the ones taken from mature plants. The loss of rooting capability depends upon the potato cultivar.展开更多
According to previous analysis, some properties bounding up with tuber yield were investigated.The results showed that tuber average weight, plastid Mg2+ -ATPase activity, plastid Ca2+ -ATPase activity,mitochondria Mg...According to previous analysis, some properties bounding up with tuber yield were investigated.The results showed that tuber average weight, plastid Mg2+ -ATPase activity, plastid Ca2+ -ATPase activity,mitochondria Mg2+-ATPase activity, total soluble protein content, tuber average diameter, and Q-enzyme activity were important factors determining the tuber yield. The linear regression equation was:Y = 0.5211 +0.0595X(1) + 0.8389X(2) + 0.0882X(3) - 0. 0073X(4) + 0. 1449X(5) + 0. 3510X(6) + 0. 0031X(7) -0.00003X(8) + 0.3412X(9)+ 0.0127X(10) + 0.2904X(11) + 0.0570X(12) + 0.0159X(13) + 0.3585X(14)+ 0.0134X(15) -0.1012X(16). At the same time, the relation between several important properties and soluble protein fractions were analyzed.展开更多
Potato crop is the fourth main food crops in the world after maize, rice and wheat. It is characterized by specific temperature requirements and develops best at about 20°C. Forecasts of global warming prompt us ...Potato crop is the fourth main food crops in the world after maize, rice and wheat. It is characterized by specific temperature requirements and develops best at about 20°C. Forecasts of global warming prompt us to study the tolerance of potato genotypes to heat during the growing season. The aim of this work was to assess the response of chosen potato cultivars to high temperature during the different stages of plant growth under conditions of good soil moisture and drought. The impact of high temperature 32°C/25°C on potato plants was determined in pot experiment in three growth stages. A main measure of tolerance of the potato cultivars to high temperature during the growing season was an evaluation of the yield in relation to the Control combination. Here we demonstrated that tested potato cultivar’s response to high temperature during the growing season is dependent on the growth stage. The earlier it occurs, the more negative its impact on the growth and yield of potatoes is.展开更多
Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters....Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters. The range of variation for all the characters were found to be wide and pronounced which indicated that characters were quantitative in nature and were under polygenic control. For all the characters, greater portion of the total δ2p was mostly contributed by the δ2g. Among the seven characters, tuber weight per plant showed the highest δ2g, PCV, GCV, H2b, GA and expected GA%. On the other hand, dry matter contents (%) showed the lowest H2b. Phenotypic and genotypic correlation coefficients of plant height with no. of tubers per plant and tuber weight per plant; no. of branches per plant with no. of tubers per plant and tuber weight per plant were found to be significant and positive.展开更多
The present investigation was undertaken to find out the best medium composition for medium-term preservation of disease free potato shoot tips in in vitro system.Thirteen potato genotypes and fourteen treatments were...The present investigation was undertaken to find out the best medium composition for medium-term preservation of disease free potato shoot tips in in vitro system.Thirteen potato genotypes and fourteen treatments were taken under consideration for the present experiment.Among fourteen treatments,mannitol and sorbitol containing media proved to be the best for medium-term preservation of potato shoot tips.High concentration of mannitol delayed root formation.展开更多
基金funded by the Scientific Research Fund of College of Science&Technology,Ningbo University for the Introduction of High-level Talents,China(RC190006)。
文摘Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.
基金supported by the National 973 Program of China (2006CB708200)Gansu Province Key Technologies R&D Program (2GS054-A41-00501),Chinathe President Youth Fund of Academy of Agri-Sciences Anhui Province, China (200933)
文摘Aquaporin (AQP) belongs to a highly conserved group of membrane proteins considered as major intrinsic proteins, which facilitate water transport across biological membranes. The discovery of AQPs in plants has resulted in a paradigm shift in the understanding of plant-water relations, however, the potential relationship between the role of aquaporins in regulating plant water balance and drought tolerance still remains elusive. In this study, the gene encoding potato AQP cDNA, StPIP1 (GenBank accession no. DQ999080), was cloned from the leaf of potato cultivar Gannongshu 2 by reverse transcription-PCR (RT-PCR). Sequence alignment was made by BLASTn in GenBank, the phylogenetic analysis was conducted using PHYLIPWY, the 3D structure was predicted in Swiss-Model server. Subcellular localization of StPIP1 was performed by constructing CaMV35S-StPIP1-GFP and rd29A-StPIP1-GFP fusion proteins and transient expression in onion epidermis. To understand StPIP1 physiological functions in potato under various stress conditions, the StPIP1 gene in a reverse orientation was transformed into tobacco driven by the Cauliflower mosaic virus (CMV) 35S promoter. The expression levels of transgenic and wild-type plants were assessed under various abiotic stress conditions using semi-quantitative RT-PCR, and the morphological and physiological responses of transgenic plants to different stress conditions were investigated. The expression of StPIP1 mRNA decreased in transgenic plants under non-stress and stress conditions, however, the reduction was more severer under drought stress. In both non-stress and stress conditions, StPIP1 was expressed predominantly in root. The morphological and physiological investigation showed no significant differences in growth rate, germination rate, and root fresh weight (FW) between transgenic and wild-type plants when grown under favorable conditions. In contrast, under drought stress, the reduction in StPIP1 expression leads to a delay in seed germination and seedling growth, accelerated seedling wilt, and leaf morphological abnormity. Under "enough" water conditions (i.e., water culture), the aerial parts of anti-sense plants showed no differences. However, for the aerial parts to accumulate the same amount of biomass, transgenic plants needed about 3 times more abundant root system to transport water for plant growth than wild-type plants. Morphological investigation showed that the reduction in StPIP1 expression increased the root system in transgenic plants under drought stress. As a result, the increase of root mass might compensate the reduced cellular water permeability in order to ensure a sufficient water supply for the plant. Results demonstrated that StPIP1 plays an important role for water transportation in potato, especially under drought stress conditions. The reduced expression of StPIP1 decreases the cellular water transport and influences the expression of endogenous AQPs genes and thereby, has impacts on seed germination, seedling growth, and stress responses of potato to drought conditions.
基金Supported by the Science and Technology Project for Agriculture Development of Shanghai Agricultural Commission[Hu Nong Ke Tui Zi(2019)No.1-8]Science and Technology Innovation Action Plan of the Science and Technology Commission of Shanghai Municipality(19DZ1203501)。
文摘[Objectives]To explore the planting adaptability of vegetables in Shanghai.[Methods]In this paper,cowpea(Vigna unguiculate(L.)Walp.),cucumber(Cucumis sativus L.),eggplant(Solanum melongena L.)and potato(Solanum tuberosum L.)were selected as experimental materials and planted in the open air.The growth status,the occurrence of diseases and insect pests,and the taste evaluation of these four kinds of common vegetables were mainly studied.[Results]The results showed that the four kinds of common vegetables in Shanghai had strong growth,strong adaptability,less pests and diseases,and good taste.[Conclusions]The cowpea,cucumber,eggplant,and potato are suitable for planting in Shanghai.
文摘将1.4kb Class I patatin基因的5′侧翼区与GUS基因融合,构建了双元表达载体pPATIs(含patatin部分信号顺序)和pPATI(不含patatin部分信号顺序)。pPATI通过基因枪介导在块茎切片中获得了瞬间表达。以上建构物通过农杆菌介导转入了马铃薯品种Desiree。X-Gluc染色(PATIs不能染色)及PCR结果证实已获得转基因植株。利用离体块茎诱导系统,GUS的表达进一步用荧光进行定量检测,结果显示,PATI-GUS的转基因植株中GUS比活性均以块茎明显高于茎段,达10-20倍。蔗糖浓度的升高,PATI-GUS植株中的GUS比活性无明显变化,与前人报道有不同。此外,光照促进PATI-GUS的表达。
基金CAPES(Coordination for the Improvement of Higher Education Personnel)for granting research scholarshipCNPq(National Council for Scientific and Technological Development)for partially funding the Potato Breeding and Genetics Program and for granting research scholarships.
文摘The production of mini-tubers under soilless cultivation system increased the availability of pathogen-free seed potatoes. The objective of this study was to evaluate the rooting capability of mini-cuttings of potato clones as a function of the irrigation solution and the physiological age of the mother plant, under soilless cultivation system. Potato mini-cuttings were collected from young and mature plants of the cultivars Asterix and Macaca and the advanced clone SMINIA 793101-3. The young plants were newly acclimatized individuals and the mature plants were at the stage of producing mini-tubers. In the first experiment, mini-cuttings originating from summer pruning of mature plants were grown under closed soilless cultivation system irrigated with a nu- trient solution developed for the production of potato mini-tubers and a solution comprised of tap water only. The experiment was conducted in a 3 × 2 factorial arrangement (clones and irrigation solutions) in the complete random design with four replicates of 15 mini-cuttings. In the second experiment, the young and mature mini-cuttings were established under a closed soilless cultivation system irrigated with nutrient solution. The experiment was conducted in a 3 × 2 factorial arrangement (clones and physiological age of the mother plant) in the complete random design with four replicates of 15 mini-cuttings. In both experiments, the percentage of rooting and survival of mini-cuttings, number of roots and length of the longest root were assessed at 21 days of cultivation. Potato mini-cuttings can be rooted under closed soilless cultivation system irrigated with nutrient solution or tap water. Mini-cuttings of young plants have higher rooting capability compared to the ones taken from mature plants. The loss of rooting capability depends upon the potato cultivar.
基金the National Natural Science Foundation(39370486) the Laboratory of Vegetative Genetics and Physiology inthe Agricultural Ministry of P.R.China.
文摘According to previous analysis, some properties bounding up with tuber yield were investigated.The results showed that tuber average weight, plastid Mg2+ -ATPase activity, plastid Ca2+ -ATPase activity,mitochondria Mg2+-ATPase activity, total soluble protein content, tuber average diameter, and Q-enzyme activity were important factors determining the tuber yield. The linear regression equation was:Y = 0.5211 +0.0595X(1) + 0.8389X(2) + 0.0882X(3) - 0. 0073X(4) + 0. 1449X(5) + 0. 3510X(6) + 0. 0031X(7) -0.00003X(8) + 0.3412X(9)+ 0.0127X(10) + 0.2904X(11) + 0.0570X(12) + 0.0159X(13) + 0.3585X(14)+ 0.0134X(15) -0.1012X(16). At the same time, the relation between several important properties and soluble protein fractions were analyzed.
文摘Potato crop is the fourth main food crops in the world after maize, rice and wheat. It is characterized by specific temperature requirements and develops best at about 20°C. Forecasts of global warming prompt us to study the tolerance of potato genotypes to heat during the growing season. The aim of this work was to assess the response of chosen potato cultivars to high temperature during the different stages of plant growth under conditions of good soil moisture and drought. The impact of high temperature 32°C/25°C on potato plants was determined in pot experiment in three growth stages. A main measure of tolerance of the potato cultivars to high temperature during the growing season was an evaluation of the yield in relation to the Control combination. Here we demonstrated that tested potato cultivar’s response to high temperature during the growing season is dependent on the growth stage. The earlier it occurs, the more negative its impact on the growth and yield of potatoes is.
文摘Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters. The range of variation for all the characters were found to be wide and pronounced which indicated that characters were quantitative in nature and were under polygenic control. For all the characters, greater portion of the total δ2p was mostly contributed by the δ2g. Among the seven characters, tuber weight per plant showed the highest δ2g, PCV, GCV, H2b, GA and expected GA%. On the other hand, dry matter contents (%) showed the lowest H2b. Phenotypic and genotypic correlation coefficients of plant height with no. of tubers per plant and tuber weight per plant; no. of branches per plant with no. of tubers per plant and tuber weight per plant were found to be significant and positive.
文摘The present investigation was undertaken to find out the best medium composition for medium-term preservation of disease free potato shoot tips in in vitro system.Thirteen potato genotypes and fourteen treatments were taken under consideration for the present experiment.Among fourteen treatments,mannitol and sorbitol containing media proved to be the best for medium-term preservation of potato shoot tips.High concentration of mannitol delayed root formation.
