In this present study, the effect of various carbon sources such as sucrose, glucose, fructose and maltose was investigated on in vitro shoot regeneration of Solanum viarum using axillary bud explants. The frequency, ...In this present study, the effect of various carbon sources such as sucrose, glucose, fructose and maltose was investigated on in vitro shoot regeneration of Solanum viarum using axillary bud explants. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon source used. Among the different concentrations (1%-6%) of carbohydrates studied, the maximum number of shoots (22.6 ± 0.50) and shoot length (5.92 ± 0.13 cm) was obtained on MS medium supplemented with 4% (w/v) fructose. The least number of shoots (1.5 ± 0.32) was obtained on 6% maltose and the least shoot length (1.2 ± 0.23) was observed on 6% glucose. Among the four types of carbon sources that were employed in the present study, fructose at 4% proved to be better choice for multiple shoot regeneration followed by sucrose, glucose and maltose from axillary bud explants of S. viarum.展开更多
The effects of bavistin, cefotoxime and kanamycin along with the ethylene inhibitor silver thiosulphate on in vitro regeneration using the axillary buds of Solanum viarum (D.) have been investigated. Axillary bud expl...The effects of bavistin, cefotoxime and kanamycin along with the ethylene inhibitor silver thiosulphate on in vitro regeneration using the axillary buds of Solanum viarum (D.) have been investigated. Axillary bud explants when cultured on MS medium supplemented with bavistin (100 mg/l) maximum number of shoots (4.21 ± 0.83) were observed. In a separate experiment, bavistin along with growth regulators such as BAP and TDZ was added to MS medium containing sucrose (3%). Maximum number of shoots was obtained (5.55 ± 0.28) in MS medium supplemented with bavistin (100 mg/l) and BAP (2.0 mg/l). Cefotoxime and kanamycin (10-150 μM/L) were added to MS medium supplemented with BAP (2.0 mg/l) with sucrose (3%) having shown the maximum shoot regeneration (4.38 ± 0.45 and 3.4 ± 0.48) at 80 μM/L and 50 μM/L respectively. Similarly the regeneration medium was supplemented with (5.0-100 μM/L) of STS. Maximum number of shoots (4.75 ± 0.43) was seen with 40 μM/L. These plantlets were further maintained for root emergence on a rooting medium supplemented with growth regulators such as (0.5-2.0 mg/l) IAA, IBA and NAA. In MS medium supplemented with IBA (1.0 mg/l), maximum number of roots (24.3 ± 0.31) was seen with 100% regeneration. The rooted plants were acclimatized and transferred to field plots with (95%) of plant surviving in the field.展开更多
文摘In this present study, the effect of various carbon sources such as sucrose, glucose, fructose and maltose was investigated on in vitro shoot regeneration of Solanum viarum using axillary bud explants. The frequency, growth and multiplication rate were highly influenced by the type and concentration of carbon source used. Among the different concentrations (1%-6%) of carbohydrates studied, the maximum number of shoots (22.6 ± 0.50) and shoot length (5.92 ± 0.13 cm) was obtained on MS medium supplemented with 4% (w/v) fructose. The least number of shoots (1.5 ± 0.32) was obtained on 6% maltose and the least shoot length (1.2 ± 0.23) was observed on 6% glucose. Among the four types of carbon sources that were employed in the present study, fructose at 4% proved to be better choice for multiple shoot regeneration followed by sucrose, glucose and maltose from axillary bud explants of S. viarum.
文摘The effects of bavistin, cefotoxime and kanamycin along with the ethylene inhibitor silver thiosulphate on in vitro regeneration using the axillary buds of Solanum viarum (D.) have been investigated. Axillary bud explants when cultured on MS medium supplemented with bavistin (100 mg/l) maximum number of shoots (4.21 ± 0.83) were observed. In a separate experiment, bavistin along with growth regulators such as BAP and TDZ was added to MS medium containing sucrose (3%). Maximum number of shoots was obtained (5.55 ± 0.28) in MS medium supplemented with bavistin (100 mg/l) and BAP (2.0 mg/l). Cefotoxime and kanamycin (10-150 μM/L) were added to MS medium supplemented with BAP (2.0 mg/l) with sucrose (3%) having shown the maximum shoot regeneration (4.38 ± 0.45 and 3.4 ± 0.48) at 80 μM/L and 50 μM/L respectively. Similarly the regeneration medium was supplemented with (5.0-100 μM/L) of STS. Maximum number of shoots (4.75 ± 0.43) was seen with 40 μM/L. These plantlets were further maintained for root emergence on a rooting medium supplemented with growth regulators such as (0.5-2.0 mg/l) IAA, IBA and NAA. In MS medium supplemented with IBA (1.0 mg/l), maximum number of roots (24.3 ± 0.31) was seen with 100% regeneration. The rooted plants were acclimatized and transferred to field plots with (95%) of plant surviving in the field.
