Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids ...Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids and flavonoids.Modern pharmacological studies have found that the isolated compounds from S.alopecuroides have a variety of pharmacological activities.In particular,alkaloids represented by matrine,aloperine and oxymatrine have significant anti-inflammatory,anti-viral,anti-tumor,anti-bacterial and immunomodulatory activities.This paper summarized the chemical constituents and pharmacological activities of S.alopecuroides,aiming to provide the basis for further research.展开更多
Objective:To evaluate the antimicrobial activity of total alkaloids extracted from Sophorea a/opecuroides L.(TASA)against clinical isolated extended-spectrum beta-lactamases(ESBLs)producing Escherichia coli(E.coil)str...Objective:To evaluate the antimicrobial activity of total alkaloids extracted from Sophorea a/opecuroides L.(TASA)against clinical isolated extended-spectrum beta-lactamases(ESBLs)producing Escherichia coli(E.coil)strains.Methods:The antibacterial activity of TASA either itself or in combination with cefotaxime(CTX)or ceftazidime(CAZ)was investigated by using the microbroth dilution method and phenotypic confirmatory disk diffusion test against three clinical isolated ESBLs-producing E.coil strains;the interactions of TASA and CTX or CAZ were ascertained by evaluating the fractional inhibitory concentration index(FICI).]Results:The antibacterial activity of either TASA itself or in combination with CTX or CAZ was found.The minimum inhibitory concentration(MICs)of TASA against the ESBLs producing isolates was 12.5 mg/mL.In the combinations with a sub-inhibitory concentration of TASA,a synergistic effect on CTX and CAZ against the ESBLs producing isolates was observed.Similarly,the isolates exposed to lower dose of TASA yielded an increased susceptibility to CTX and CAZ by 8-16 folds determined by microdilution assay.Moreover,enzymatic detection of ESBLs demonstrated that TASA induced reversal resistance to CTX and CAZ partially by a mechanism of inhibition of ESBLs activity in these isolates.Additionally,in the tested isolates following the exposure of TASA,molecular analysis verified the SHV-type beta-lactamase encoding ESBL gene in these isolates,and no mutation was introduced into the ESBL gene.Conclusions:These results suggest that TASA could be used as a source of natural compound with pharmacological activity of reversal resistance to antimicrobial agent.These findings also indicated that the application of the TASA in combination with antibiotics might prove useful in the control and treatment of infectious diseases caused by the ESBLs producing enterobacteriaceae.展开更多
Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB ir...Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB irradiation. The results showed that the extracts had strong UVA and UVB absorption ability and hydroxyl free radical scavenging ability. The S. japonica L. polysaccharide showed strong anti-UVB irradiation ability according to the cell viability experiment. It suggests that the H. cordata flavonoid and S. japonica L. polysaccharide could be combined as a candidate of anti-UV sun-screening supplement and compensate for the shortcomings of chemical sunscreens against UVA ray.展开更多
基金the College Student Innovation and Entrepreneurship Training Program(X202010163166).
文摘Sophora alopecuroides L.,a perennial plant belonging to Leguminosae family,is used as medicinal materials to treat a variety of skin diseases.Its chemical components are complex and diverse,mainly including alkaloids and flavonoids.Modern pharmacological studies have found that the isolated compounds from S.alopecuroides have a variety of pharmacological activities.In particular,alkaloids represented by matrine,aloperine and oxymatrine have significant anti-inflammatory,anti-viral,anti-tumor,anti-bacterial and immunomodulatory activities.This paper summarized the chemical constituents and pharmacological activities of S.alopecuroides,aiming to provide the basis for further research.
基金Supported by a Sub-project of National Basic Research Program of China(No.2006CB504401)the National Natural Science Foundation of China(No.31060348,No.30860207)Grants of Science and Technology Program of Ningxia to Yujiong Wang(No.Z2006-1-75001,KGZ-12-10-02)
文摘Objective:To evaluate the antimicrobial activity of total alkaloids extracted from Sophorea a/opecuroides L.(TASA)against clinical isolated extended-spectrum beta-lactamases(ESBLs)producing Escherichia coli(E.coil)strains.Methods:The antibacterial activity of TASA either itself or in combination with cefotaxime(CTX)or ceftazidime(CAZ)was investigated by using the microbroth dilution method and phenotypic confirmatory disk diffusion test against three clinical isolated ESBLs-producing E.coil strains;the interactions of TASA and CTX or CAZ were ascertained by evaluating the fractional inhibitory concentration index(FICI).]Results:The antibacterial activity of either TASA itself or in combination with CTX or CAZ was found.The minimum inhibitory concentration(MICs)of TASA against the ESBLs producing isolates was 12.5 mg/mL.In the combinations with a sub-inhibitory concentration of TASA,a synergistic effect on CTX and CAZ against the ESBLs producing isolates was observed.Similarly,the isolates exposed to lower dose of TASA yielded an increased susceptibility to CTX and CAZ by 8-16 folds determined by microdilution assay.Moreover,enzymatic detection of ESBLs demonstrated that TASA induced reversal resistance to CTX and CAZ partially by a mechanism of inhibition of ESBLs activity in these isolates.Additionally,in the tested isolates following the exposure of TASA,molecular analysis verified the SHV-type beta-lactamase encoding ESBL gene in these isolates,and no mutation was introduced into the ESBL gene.Conclusions:These results suggest that TASA could be used as a source of natural compound with pharmacological activity of reversal resistance to antimicrobial agent.These findings also indicated that the application of the TASA in combination with antibiotics might prove useful in the control and treatment of infectious diseases caused by the ESBLs producing enterobacteriaceae.
基金Supported by College Students'Practice Innovation Training Project in Henan Province(No.201711834006)Henan Provincial Department of Education(No.15A350008)
文摘Houttuynia cordata flavonoid and Sophora japonica L. polysaccharide were investigated to develop anti-UV sun-screening agent through UV wavelength scanning spectrum, antioxidant ability and cell viability after UVB irradiation. The results showed that the extracts had strong UVA and UVB absorption ability and hydroxyl free radical scavenging ability. The S. japonica L. polysaccharide showed strong anti-UVB irradiation ability according to the cell viability experiment. It suggests that the H. cordata flavonoid and S. japonica L. polysaccharide could be combined as a candidate of anti-UV sun-screening supplement and compensate for the shortcomings of chemical sunscreens against UVA ray.
