Abiotic stresses, such as drought, salt, extreme temperatures, and heavy metal pollution, are the main environmental factors that limit crop growth and yield. Sorghum, a C4 grass plant with high photosynthetic efficie...Abiotic stresses, such as drought, salt, extreme temperatures, and heavy metal pollution, are the main environmental factors that limit crop growth and yield. Sorghum, a C4 grass plant with high photosynthetic efficiency, can grow in adverse environmental conditions due to its excellent stress resistance characteristics. Therefore, unraveling the stress-resistance mechanism of sorghum could provide a theoretical basis for developing and cultivating various stress-resistant crops. This understanding could also help to create a conducive environment for using marginal soil in agriculture and ensuring food security. In this review, we discuss the adaptation mechanisms of sorghum under drought, salinity, temperature, and soil heavy metal stresses, the specific response to stress, the screening of sorghum-resistant germplasm, and the identification and functional analysis of the relevant genes and quantitative trait loci(QTL). In addition, we discuss the application potential of different stress-tolerant sorghum germplasms reported to date and emphasize the feasibility and potential use in developing and promoting highly stress-tolerant sorghum in marginal soil.展开更多
Sorghum(Sorghum bicolor(L.)Moench)is a world cereal crop used in China for producing Baijiu,a distilled spirit.We report a telomere-to-telomere genome assembly of the Baijiu cultivar Hongyingzi,HYZ-T2T,using ultralong...Sorghum(Sorghum bicolor(L.)Moench)is a world cereal crop used in China for producing Baijiu,a distilled spirit.We report a telomere-to-telomere genome assembly of the Baijiu cultivar Hongyingzi,HYZ-T2T,using ultralong reads.The 10 chromosome pairs contained 33,462 genes,of which 93%were functionally annotated.The 20 telomeres and 10 centromeric regions on the HYZ-T2T chromosomes were predicted and two consecutive large inversions on chromosome 2 were characterized.A 65-gene reconstruction of the metabolic pathway of tannins,the flavor substances in Baijiu,was performed and may advance the breeding of sorghum cultivars for Baijiu production.展开更多
Sorghum is a vital commodity and greatly contributes to the daily calorie needs for millions of the inhabitants in Senegal, West Africa. Yet, sorghum productivity and profitability are impacted by diseases. In the 202...Sorghum is a vital commodity and greatly contributes to the daily calorie needs for millions of the inhabitants in Senegal, West Africa. Yet, sorghum productivity and profitability are impacted by diseases. In the 2022 growing season, 122 farmers’ fields across 7 regions, notably Diourbel, Fatick, Kaffrine, Kaolack, Kolda, Tambacounda, and Thies were surveyed for foliar and panicle diseases. During the survey, stops were made at 30 km intervals and at each stop, 2 - 5 fields were evaluated. In each field, 40 plants mostly at soft to early hard dough stages of development were assessed using a W-shaped pattern to cover the whole field. A total of 13 diseases, including leaf blight, anthracnose, Zonate leaf spot, Sooty stripe, rough leaf spot, oval leaf spot, long smut, grain mold, and covered kernel smut were documented. The most predominant diseases were leaf blight, anthracnose, and zonate leaf spot. The prevalence of leaf blight was 100%, while anthracnose and zonate leaf spot were found in 93 out of the 122 fields surveyed, indicating a 76% prevalence, respectively. Across the regions, the prevalence of rough leaf spot was 47%, covered kernel smut (32%), oval leaf spot (19%) and target leaf spot (19%). Mean incidence of leaf blight was high in all regions, ranging from 94% (Kaolack) to 100% (Fatick and Tambacounda). The highest mean incidence of anthracnose (62%) and covered kernel smut (16%) was noted in Tambacounda region. In the region of Thies, the highest mean incidence of zonate leaf spot (49%) was recorded. The mean severity of leaf blight (37%) was highest on plants assessed in the region of Kaffrine, followed by those in the regions of Kaolack, Tambacounda, and Kolda. Anthracnose infection was most intense on plants evaluated in Kolda, while zonate leaf spot was most severe in the region of Tambacounda. Fields with incidences of 85% and above were considered as “hotspots” to evaluate sorghum germplasm for disease resistance. This survey is significant because the information obtained will be beneficial to plant pathologists, sorghum producers, government officials, and funding agencies to prioritize research projects that ensure productivity and food security.展开更多
Grain mold, associated with many fungi, is the most important disease of sorghum, causing both yield and quality losses. In this study, 23 sorghum differentials used in pathotype characterization of anthracnose and he...Grain mold, associated with many fungi, is the most important disease of sorghum, causing both yield and quality losses. In this study, 23 sorghum differentials used in pathotype characterization of anthracnose and head smut pathogens were evaluated for grain mold resistance under favorable conditions in Isabela, Puerto Rico. Lines BTx643 and IS18760 exhibited the lowest grain mold severity, indicating that these two may possess genes for grain mold resistance. These two lines also recorded the highest germination rates 94.7% and 97.6%, respectively, and their seed weight was among the heaviest. In conclusion, these two lines can be utilized in breeding programs to develop grain mold-resistant hybrid lines.展开更多
Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological re...Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic stresses.It plays a key role in distributing carbohydrates and sugar signal transporting.In the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of cereals.Expression results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in inflorescences.Results of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some organelles.Besides,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.展开更多
Sorghum[Sorghum bicolor(L.)Moench],a multipurpose C4 crop,is also a model species of the Poaceae family for plant research.During the process of domestication,the modification of seed dispersal mode is considered a ke...Sorghum[Sorghum bicolor(L.)Moench],a multipurpose C4 crop,is also a model species of the Poaceae family for plant research.During the process of domestication,the modification of seed dispersal mode is considered a key event,as the loss of seed shattering caused a significant increase in yield.In order to understand the seed shattering process in sorghum,we further studied eight previously identified divergent sorghum germplasm with different shattering degrees.We described their phenotypes in great detail,analyzed the histology of abscission zone,and conducted a gene co-expression analysis.We observed that the abscission layer of the most strong-shattering varieties began to differentiate before the 5-10 cm panicles development stage and was completely formed at flag leaf unfolding.The protective cells on the pedicels were also fully lignified by flowering.Through the weighted gene correlation network analysis(WGCNA),we mined for candidate genes involved in the abscission process at the heading stage.We found that these genes were mainly associated with such biological processes as hormone signal transmission(SORBI_3003G361300,SORBI_3006G216500,SORBI_3009G027800,SORBI_3007G077200),cell wall modification and degradation(SORBI_3002G205500,SORBI_3004G013800,SORBI_3010G022400,SORBI_3003G251800,SORBI_3003G254700,SORBI_3003G410800,SORBI_3009G162700,SORBI_3001G406700,SORBI_3004G042700,SORBI_3004G244600,SORBI_3001G099100),and lignin synthesis(SORBI_3004G220700,SORBI_3004G062500,SORBI_3010G214900,SORBI_3009G181800).Our study has provided candidate genes required for shedding for further study.We believe that function characterization of these genes may provide insight into our understanding of seed shattering process.展开更多
Sorghum(Sorghum bicolor(L.) Moench) is a major world crop that is a reliable source of fodder and food grain in arid regions. However, unlike other cereals, sorghum grain has low nutritional value, owing mainly to the...Sorghum(Sorghum bicolor(L.) Moench) is a major world crop that is a reliable source of fodder and food grain in arid regions. However, unlike other cereals, sorghum grain has low nutritional value, owing mainly to the resistance of its storage proteins(kafirins) to protease digestion. Changing the composition of kafirins or their primary structure may address this problem. To induce mutations in kafirin-encoding genes that were expected to disturb their accumulation in endosperm cells, we used a genome-editing approach. By Agrobacterium-mediated genetic transformation of immature embryos of cv. Avans, we obtained 14 transgenic plants with genetic constructs for site-directed mutagenesis of the k1C5 and g KAF1 genes encoding 22 k Da a-and 28 kDa γ-kafirins, respectively. Sequencing of 5 regenerants obtained by using k1C5-addressing vector revealed two plants with mutations. T_1 progeny of these mutants had higher in vitro digestibility of endosperm proteins(86%–92%), in comparison with the donor Avans(63%–67%). The kernels of these plants had a thick vitreous endosperm. A mutant with increased in vitro protein digestibility and vitreous endosperm, carrying a mutation in the target sequence, was also obtained by use of the gKAF1-addressing vector. Thus, using genome editing technology, we have obtained mutants with improved kafirin digestibility that can be used in sorghum breeding.展开更多
Salt stress is one of the major limitations to modern agriculture that negatively influences plant growth and productivity.Salt tolerant cultivar can provide excellent solution to enhance stress tolerance with plantfitn...Salt stress is one of the major limitations to modern agriculture that negatively influences plant growth and productivity.Salt tolerant cultivar can provide excellent solution to enhance stress tolerance with plantfitness to unfavorable environments.Therefore,this study was aimed to screen salt tolerant sorghum genotypes through evaluating of different morphological,biochemical,and physiological attributes in response to salinity stress.In this study,we have been evaluated total six sorghum genotypes including Hybrid sorgo,Debgiri,BD-703,BD-706,BD-707,and BD-725 under salt stress(12 dS m^(-1) NaCl).The response variables included length and weight of root and shoot,root:shoot ratio(RSR),photosynthesis(A),transpiration rate(E),elemental concen-trations(K^(+),Na^(+) and K^(+)/Na^(+)),photochemical efficiency of photosystem II(F_(v)/F_(m)),water use efficiency(WUE)and pigment content(chlorophyll a,and b).The results revealed that saline environment significantly reduced all response variables under study of sorghum genotypes,however,Hybrid sorgo remained unmatched by recording the maximum root and shoot traits.The same genotype recorded higher photosynthetic efficiency which was attributed to Na^(+) extrusion,K^(+) uptake and higher K^(+)/Na^(+) ratio(1.8 at stress),while these mechanisms were not fully active in rest of genotypes.Moreover,this study also implies the involvement of proline in imparting tolerance against saline environment in Hybrid sorgo genotype.Overall,BD-703 remained the most salt sensitive genotype as evident from the minimum morphological growth traits and the least biosynthesis of osmoprotectants.Thesefindings open new research avenues for salt stress alleviation by identifying elite salt-to-lerant genotypes of sorghum for breeding programs.展开更多
Flour serves as one of the primary ingredients of cookies,which affects dough properties,physical changes during baking,and post-baking cookie properties.Two gluten-free cookie formulations were developed with the goa...Flour serves as one of the primary ingredients of cookies,which affects dough properties,physical changes during baking,and post-baking cookie properties.Two gluten-free cookie formulations were developed with the goal of use as inclusions in frozen desserts.Blonde and chocolate cookies were made with sorghum flour or all-purpose wheat flour and then evaluated for physical and chemical properties.To determine functionality in a frozen state,texture analysis was conducted on cookies at−30℃,−17°C,and 20℃,replicating various ice cream storage temperatures.To simulate the process effect of incorporating the product into a frozen dessert,cookies were crumbled mechanically,and the crumbles were separated based on size.When evaluating cookies,no significant differences(P<0.05)were observed in width(W),thickness(T),spread,and W/T ratio for flour source within formula.Blonde cookies had lower moisture contents than chocolate cookies;further,blonde cookies made from sorghum flour had less moisture than those made with wheat flour.As the cookie temperature decreased from 20℃to−17℃,cookie hardness increased,with flour type having no effect.Within formula,cookies had similar instrumental color parameters.However,blonde cookies made from sorghum flour produced more smaller-sized pieces than those made from wheat flour.The results of this study demonstrate that sorghum flour cookies have desirable properties for use as inclusions in ice cream and other frozen desserts.展开更多
Aim of this study is to see how ultrasound(US)and microwave(MW)pretreatments affectγ-aminobutyric acid(GABA)and physicochemical parameters of sorghum sprouts.Before sprouting,the sorghum was treated with the US at di...Aim of this study is to see how ultrasound(US)and microwave(MW)pretreatments affectγ-aminobutyric acid(GABA)and physicochemical parameters of sorghum sprouts.Before sprouting,the sorghum was treated with the US at different time intervals(10,15,20 min)and MW at various power levels(10%,30%,50%).Sample treated by the US for 15 min showed the highest sprouting percentage of 97.33%.Both treatments induced stress in grain resulting in GABA accumulation substantially from the control where US for 15 min and MW at 10%power level had the highest of 87.14 and 66.97μg/g,respectively.US treatment for 20 min showed the highest total phenolic content(TPC)of 21.26 mg GAE/100 g,while on 10 min of treatment,it showed the highest antioxidant activity of 84.53%DPPH inhibition.The water absorption capacity and oil absorption capacity were the highest of 0.86 g/g and 0.98 g/g in the MW-treated sample at 10%output power respectively.In conclusion,the ultrasound treatment successfully accelerated the sprouting rate and GABA content while retaining total phenolic content,making it beneficial for developing functional foods.展开更多
Sorghum bicolor is a multi-purpose species adapted to many agroecological zones of Burkina Faso. In the local farming system, different types of sorghum are cultivated together. Such farming conditions could increase ...Sorghum bicolor is a multi-purpose species adapted to many agroecological zones of Burkina Faso. In the local farming system, different types of sorghum are cultivated together. Such farming conditions could increase gene flow between the different types of sorghum and contribute to the evolution of their main morphological characteristics. Understanding the effect of the farming system on the characteristics of different types of sorghum could contribute to building a strategy for the management of its genetic diversity. A survey and accessions collection was carried out in ten villages selected in two agroclimatic zones of Burkina Faso. A total of 133 accessions were collected and thirty local names were recorded. The results revealed a significant variability within sorghum characteristics based on farmers’ descriptions. Four types of sorghum were identified by farmers based on their uses. These are sweet grain sorghum, grain sorghum, sweet sorghum and dyer sorghum. Most of farmers (54.6%) practised intercropping, and 28.86% of them intercropped several types of sorghum in the same or nearby fields. A high coincidence of the flowering period of the types of sorghum was observed by farmers in both agroclimatic zones. The results also showed that most of the farmers (55.7%) noted changes in the morphological characteristics of different types of sorghum. These variations included the reduction in potential yields and changes in grain taste and colour within the same type. Understanding these variations could help conserve and sustain sorghum genetic resources in Burkina Faso.展开更多
In order to find out the effects of soil fluorine on ecological environment and crop safety in the sorghum cultivation base of Renhuai City, Guizhou Province, the content and the occurrence forms of soil fluorine and ...In order to find out the effects of soil fluorine on ecological environment and crop safety in the sorghum cultivation base of Renhuai City, Guizhou Province, the content and the occurrence forms of soil fluorine and its influencing factors of Maoba Town were investigated and studied. The results showed that the total fluorine content in the soil of the sorghum base ranged from 668.60 to 2 596.80 mg/kg, with an average of 1 483.25 mg/kg, which was 3.10 and 1.85 times of the national soil background value and the average value of the soil in endemic areas, respectively. The spatial distribution of fluorine in the soil of the study area was uneven, and the fluorine content was in the order of Anliang Village>Yangliu Village>Houba Village>Xiongfeng Village. The soil fluorine contents of various forms varied greatly, and the distribution law was as follows: residual state>water soluble state>organically bound state>exchangeable state>Fe/Mn-F state. The residual fluorine content was the highest, accounting for more than 99% of total fluorine content, while the sum of other four forms was less than 1%, with their respective proportions ranging from 0.10% to 0.30%. The content and proportion of available fluorine in water-soluble and exchangeable states were relatively low, which had limited influence on the surrounding water environment and crop safety. The physicochemical properties of soil, such as pH, organic matter, cation exchange capacity and clay particles had a certain influence on the occurrence forms of soil fluorine, thus changing the availability and migration of soil fluorine, which might endanger the ecological environment safety, food safety and human health.展开更多
Participatory varietal selection (PVS) with farmers and Seed Companies was conducted at Maradi research station (07°05'E/13°48'N) and in farmer field to evaluate and select sorghum hybrid varieties f...Participatory varietal selection (PVS) with farmers and Seed Companies was conducted at Maradi research station (07°05'E/13°48'N) and in farmer field to evaluate and select sorghum hybrid varieties for high yield and other important agronomic traits. The experimental design was a randomized complete block with three replications where flowering (days), plant height (cm) and yield (kg) were collected. The analysis of variance showed highly significance among treatments of all traits measured Environment and Genotype by Environment interactions also contributed significantly to the performance of yield components. The highest average grain yield was recorded from hybrids P9511A x ST9007-5-2-1 (4289 kg/ha), NE223A x 90SN1 (3666 kg/ha), NE223A x Sepon 82 (3533 kg/ha) and NE223A x P9405 (3519 kg/ha) across locations. Farmers’ preferences were the panicle size, good seed set, earliness, and seed color. Hence, in a variety selection farmer’s preferences focus more on prioritized yield-related trait. The best varieties ranked by traits of interest were P9511A x ST9007-5-2-1, NE223A x 90SN1, NE223A x P9405 and P9511A x SEPON 82 that performed well under their circumstances. The results showed that farmers’ preferred varieties match with researchers. Therefore, based on objectively measured traits, farmers’ preferences and the agro ecologies of the site, varieties NE223A x 90SN1 and P9511A x ST9007-5-2-1 were found promising for production.展开更多
This research aimed at testing the viability of using Sorghum Stalk Ash (SSA) as a partial replacement of lime in the stabilization of red clay soils for road subgrade construction. Red clay soils have been identified...This research aimed at testing the viability of using Sorghum Stalk Ash (SSA) as a partial replacement of lime in the stabilization of red clay soils for road subgrade construction. Red clay soils have been identified as highly expansive soils, which are affected by both climatic conditions and loading patterns. The consideration of both traffic loading patterns and climatic effects on these soils has been taken into account. A penetration test of 2.5 mm has been used on both pure red soils and stabilized soils at 10% and 15% partial replacement of lime with SSA and showed an improvement in the CBR of stabilized red clay soils up to 11.6%. Again, the PI of stabilized soils at 15% partial replacement of lime reduced up to 11.2%. The results obtained on both CBR and PI of these red clay soils are within the recommended values for the effective subgrade required for laying both permanent and flexible pavements. As a result, a recommendation of making use of SSA to lower the quantities of lime and its costs used in the stabilization of highly expansive soils have been tested through this research. However, further research on a more percentage partial replacement of lime to improve the PI of these soils to below 10% while keeping the CBR levels within the road construction regulations is welcomed.展开更多
Sorghum is an important source of food, feed and raw material for brewing, and is expected to be a promising bioenergy crop. Sorghum is well known for its strong resistance to abiotic stress and wide adaptability, and...Sorghum is an important source of food, feed and raw material for brewing, and is expected to be a promising bioenergy crop. Sorghum is well known for its strong resistance to abiotic stress and wide adaptability, and salt tolerance is one of its main characteristics. Increasing sorghum planting acreage on saline-alkalien land is one way to effectively use this kind of marginal soil. In this paper, domestic and overseas research on plant tolerance to soil salinity and alkalinity in sorghum, including salt-tolerant genetics and breeding, physiology, cultivation, and identification of tolerant germplasms, are reviewed. Suggestions for further studies on salinity and alkalinity tolerance in sorghum are given, and the prospects for sorghum production in saline-alkalien land are discussed.展开更多
基金financial support from the National Key R&D Program of China(2022YFD1201702)the National Natural Science Foundation of China(32272040)the Agricultural Fine Seed Project of Shandong Province,China(2021LZGC006)。
文摘Abiotic stresses, such as drought, salt, extreme temperatures, and heavy metal pollution, are the main environmental factors that limit crop growth and yield. Sorghum, a C4 grass plant with high photosynthetic efficiency, can grow in adverse environmental conditions due to its excellent stress resistance characteristics. Therefore, unraveling the stress-resistance mechanism of sorghum could provide a theoretical basis for developing and cultivating various stress-resistant crops. This understanding could also help to create a conducive environment for using marginal soil in agriculture and ensuring food security. In this review, we discuss the adaptation mechanisms of sorghum under drought, salinity, temperature, and soil heavy metal stresses, the specific response to stress, the screening of sorghum-resistant germplasm, and the identification and functional analysis of the relevant genes and quantitative trait loci(QTL). In addition, we discuss the application potential of different stress-tolerant sorghum germplasms reported to date and emphasize the feasibility and potential use in developing and promoting highly stress-tolerant sorghum in marginal soil.
基金supported by the Scientific Research Project of Kweichow Moutai Liquor Co.,Ltd.(MTGF2023007)the National Natural Science Foundation of China(32160459,32172036)+2 种基金the Guizhou Natural Science Foundation of China(QKHJC[2023]YB169)the Innovation Capacity Building Project of Guizhou Scientific Institutions(QKFQ[2022]007])the Guizhou Academy of Agricultural Sciences Project(Guizhou Agricultural Germplasm Resources(2023)06)。
文摘Sorghum(Sorghum bicolor(L.)Moench)is a world cereal crop used in China for producing Baijiu,a distilled spirit.We report a telomere-to-telomere genome assembly of the Baijiu cultivar Hongyingzi,HYZ-T2T,using ultralong reads.The 10 chromosome pairs contained 33,462 genes,of which 93%were functionally annotated.The 20 telomeres and 10 centromeric regions on the HYZ-T2T chromosomes were predicted and two consecutive large inversions on chromosome 2 were characterized.A 65-gene reconstruction of the metabolic pathway of tannins,the flavor substances in Baijiu,was performed and may advance the breeding of sorghum cultivars for Baijiu production.
文摘Sorghum is a vital commodity and greatly contributes to the daily calorie needs for millions of the inhabitants in Senegal, West Africa. Yet, sorghum productivity and profitability are impacted by diseases. In the 2022 growing season, 122 farmers’ fields across 7 regions, notably Diourbel, Fatick, Kaffrine, Kaolack, Kolda, Tambacounda, and Thies were surveyed for foliar and panicle diseases. During the survey, stops were made at 30 km intervals and at each stop, 2 - 5 fields were evaluated. In each field, 40 plants mostly at soft to early hard dough stages of development were assessed using a W-shaped pattern to cover the whole field. A total of 13 diseases, including leaf blight, anthracnose, Zonate leaf spot, Sooty stripe, rough leaf spot, oval leaf spot, long smut, grain mold, and covered kernel smut were documented. The most predominant diseases were leaf blight, anthracnose, and zonate leaf spot. The prevalence of leaf blight was 100%, while anthracnose and zonate leaf spot were found in 93 out of the 122 fields surveyed, indicating a 76% prevalence, respectively. Across the regions, the prevalence of rough leaf spot was 47%, covered kernel smut (32%), oval leaf spot (19%) and target leaf spot (19%). Mean incidence of leaf blight was high in all regions, ranging from 94% (Kaolack) to 100% (Fatick and Tambacounda). The highest mean incidence of anthracnose (62%) and covered kernel smut (16%) was noted in Tambacounda region. In the region of Thies, the highest mean incidence of zonate leaf spot (49%) was recorded. The mean severity of leaf blight (37%) was highest on plants assessed in the region of Kaffrine, followed by those in the regions of Kaolack, Tambacounda, and Kolda. Anthracnose infection was most intense on plants evaluated in Kolda, while zonate leaf spot was most severe in the region of Tambacounda. Fields with incidences of 85% and above were considered as “hotspots” to evaluate sorghum germplasm for disease resistance. This survey is significant because the information obtained will be beneficial to plant pathologists, sorghum producers, government officials, and funding agencies to prioritize research projects that ensure productivity and food security.
文摘Grain mold, associated with many fungi, is the most important disease of sorghum, causing both yield and quality losses. In this study, 23 sorghum differentials used in pathotype characterization of anthracnose and head smut pathogens were evaluated for grain mold resistance under favorable conditions in Isabela, Puerto Rico. Lines BTx643 and IS18760 exhibited the lowest grain mold severity, indicating that these two may possess genes for grain mold resistance. These two lines also recorded the highest germination rates 94.7% and 97.6%, respectively, and their seed weight was among the heaviest. In conclusion, these two lines can be utilized in breeding programs to develop grain mold-resistant hybrid lines.
基金supported by the National Natural Science Foundation of China(32001607)the Fundamental Research Funds for the Central Universities of Southwest University,China(SWU118087)。
文摘Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic stresses.It plays a key role in distributing carbohydrates and sugar signal transporting.In the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of cereals.Expression results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in inflorescences.Results of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some organelles.Besides,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.
基金supported by the National Key Research and Development Program of China(2018YFD1000706,2018YFD1000700).
文摘Sorghum[Sorghum bicolor(L.)Moench],a multipurpose C4 crop,is also a model species of the Poaceae family for plant research.During the process of domestication,the modification of seed dispersal mode is considered a key event,as the loss of seed shattering caused a significant increase in yield.In order to understand the seed shattering process in sorghum,we further studied eight previously identified divergent sorghum germplasm with different shattering degrees.We described their phenotypes in great detail,analyzed the histology of abscission zone,and conducted a gene co-expression analysis.We observed that the abscission layer of the most strong-shattering varieties began to differentiate before the 5-10 cm panicles development stage and was completely formed at flag leaf unfolding.The protective cells on the pedicels were also fully lignified by flowering.Through the weighted gene correlation network analysis(WGCNA),we mined for candidate genes involved in the abscission process at the heading stage.We found that these genes were mainly associated with such biological processes as hormone signal transmission(SORBI_3003G361300,SORBI_3006G216500,SORBI_3009G027800,SORBI_3007G077200),cell wall modification and degradation(SORBI_3002G205500,SORBI_3004G013800,SORBI_3010G022400,SORBI_3003G251800,SORBI_3003G254700,SORBI_3003G410800,SORBI_3009G162700,SORBI_3001G406700,SORBI_3004G042700,SORBI_3004G244600,SORBI_3001G099100),and lignin synthesis(SORBI_3004G220700,SORBI_3004G062500,SORBI_3010G214900,SORBI_3009G181800).Our study has provided candidate genes required for shedding for further study.We believe that function characterization of these genes may provide insight into our understanding of seed shattering process.
基金supported by the Russian Foundation for Basic Research (grant#19-016-00117)。
文摘Sorghum(Sorghum bicolor(L.) Moench) is a major world crop that is a reliable source of fodder and food grain in arid regions. However, unlike other cereals, sorghum grain has low nutritional value, owing mainly to the resistance of its storage proteins(kafirins) to protease digestion. Changing the composition of kafirins or their primary structure may address this problem. To induce mutations in kafirin-encoding genes that were expected to disturb their accumulation in endosperm cells, we used a genome-editing approach. By Agrobacterium-mediated genetic transformation of immature embryos of cv. Avans, we obtained 14 transgenic plants with genetic constructs for site-directed mutagenesis of the k1C5 and g KAF1 genes encoding 22 k Da a-and 28 kDa γ-kafirins, respectively. Sequencing of 5 regenerants obtained by using k1C5-addressing vector revealed two plants with mutations. T_1 progeny of these mutants had higher in vitro digestibility of endosperm proteins(86%–92%), in comparison with the donor Avans(63%–67%). The kernels of these plants had a thick vitreous endosperm. A mutant with increased in vitro protein digestibility and vitreous endosperm, carrying a mutation in the target sequence, was also obtained by use of the gKAF1-addressing vector. Thus, using genome editing technology, we have obtained mutants with improved kafirin digestibility that can be used in sorghum breeding.
基金This research was funded by the Researchers Supporting Project No. (RSP2023R390),King Saud University, Riyadh, Saudi Arabia.
文摘Salt stress is one of the major limitations to modern agriculture that negatively influences plant growth and productivity.Salt tolerant cultivar can provide excellent solution to enhance stress tolerance with plantfitness to unfavorable environments.Therefore,this study was aimed to screen salt tolerant sorghum genotypes through evaluating of different morphological,biochemical,and physiological attributes in response to salinity stress.In this study,we have been evaluated total six sorghum genotypes including Hybrid sorgo,Debgiri,BD-703,BD-706,BD-707,and BD-725 under salt stress(12 dS m^(-1) NaCl).The response variables included length and weight of root and shoot,root:shoot ratio(RSR),photosynthesis(A),transpiration rate(E),elemental concen-trations(K^(+),Na^(+) and K^(+)/Na^(+)),photochemical efficiency of photosystem II(F_(v)/F_(m)),water use efficiency(WUE)and pigment content(chlorophyll a,and b).The results revealed that saline environment significantly reduced all response variables under study of sorghum genotypes,however,Hybrid sorgo remained unmatched by recording the maximum root and shoot traits.The same genotype recorded higher photosynthetic efficiency which was attributed to Na^(+) extrusion,K^(+) uptake and higher K^(+)/Na^(+) ratio(1.8 at stress),while these mechanisms were not fully active in rest of genotypes.Moreover,this study also implies the involvement of proline in imparting tolerance against saline environment in Hybrid sorgo genotype.Overall,BD-703 remained the most salt sensitive genotype as evident from the minimum morphological growth traits and the least biosynthesis of osmoprotectants.Thesefindings open new research avenues for salt stress alleviation by identifying elite salt-to-lerant genotypes of sorghum for breeding programs.
基金The authors would like to extend an expression of gratitude to the USDA for analyzing particle sizes on the two flour samples.Masters'candidate Janae Brown in the Food Science Institute at Kansas State University(Manhattan,KS,USA)assisted in taking pictures for this publication.The authors acknowledge support from the Kansas State University Research and Extension,publication number 23-087-J.Mention of trade names or commercial products in this publication is solely for providing specific information and does not imply recommendation or endorsement by the U.S.Department of Agriculture.The USDA is an equal opportunity provider and employer.This work was supported in part by the U.S.Department of Agriculture,Agricultural Research Service.
文摘Flour serves as one of the primary ingredients of cookies,which affects dough properties,physical changes during baking,and post-baking cookie properties.Two gluten-free cookie formulations were developed with the goal of use as inclusions in frozen desserts.Blonde and chocolate cookies were made with sorghum flour or all-purpose wheat flour and then evaluated for physical and chemical properties.To determine functionality in a frozen state,texture analysis was conducted on cookies at−30℃,−17°C,and 20℃,replicating various ice cream storage temperatures.To simulate the process effect of incorporating the product into a frozen dessert,cookies were crumbled mechanically,and the crumbles were separated based on size.When evaluating cookies,no significant differences(P<0.05)were observed in width(W),thickness(T),spread,and W/T ratio for flour source within formula.Blonde cookies had lower moisture contents than chocolate cookies;further,blonde cookies made from sorghum flour had less moisture than those made with wheat flour.As the cookie temperature decreased from 20℃to−17℃,cookie hardness increased,with flour type having no effect.Within formula,cookies had similar instrumental color parameters.However,blonde cookies made from sorghum flour produced more smaller-sized pieces than those made from wheat flour.The results of this study demonstrate that sorghum flour cookies have desirable properties for use as inclusions in ice cream and other frozen desserts.
基金The authors would like to thank the National Institute of Food Technology,Entrepreneurship and Management,Thanjavur(NIFTEM-T),for providing the infrastructure and facilities to accomplish this research work.
文摘Aim of this study is to see how ultrasound(US)and microwave(MW)pretreatments affectγ-aminobutyric acid(GABA)and physicochemical parameters of sorghum sprouts.Before sprouting,the sorghum was treated with the US at different time intervals(10,15,20 min)and MW at various power levels(10%,30%,50%).Sample treated by the US for 15 min showed the highest sprouting percentage of 97.33%.Both treatments induced stress in grain resulting in GABA accumulation substantially from the control where US for 15 min and MW at 10%power level had the highest of 87.14 and 66.97μg/g,respectively.US treatment for 20 min showed the highest total phenolic content(TPC)of 21.26 mg GAE/100 g,while on 10 min of treatment,it showed the highest antioxidant activity of 84.53%DPPH inhibition.The water absorption capacity and oil absorption capacity were the highest of 0.86 g/g and 0.98 g/g in the MW-treated sample at 10%output power respectively.In conclusion,the ultrasound treatment successfully accelerated the sprouting rate and GABA content while retaining total phenolic content,making it beneficial for developing functional foods.
文摘Sorghum bicolor is a multi-purpose species adapted to many agroecological zones of Burkina Faso. In the local farming system, different types of sorghum are cultivated together. Such farming conditions could increase gene flow between the different types of sorghum and contribute to the evolution of their main morphological characteristics. Understanding the effect of the farming system on the characteristics of different types of sorghum could contribute to building a strategy for the management of its genetic diversity. A survey and accessions collection was carried out in ten villages selected in two agroclimatic zones of Burkina Faso. A total of 133 accessions were collected and thirty local names were recorded. The results revealed a significant variability within sorghum characteristics based on farmers’ descriptions. Four types of sorghum were identified by farmers based on their uses. These are sweet grain sorghum, grain sorghum, sweet sorghum and dyer sorghum. Most of farmers (54.6%) practised intercropping, and 28.86% of them intercropped several types of sorghum in the same or nearby fields. A high coincidence of the flowering period of the types of sorghum was observed by farmers in both agroclimatic zones. The results also showed that most of the farmers (55.7%) noted changes in the morphological characteristics of different types of sorghum. These variations included the reduction in potential yields and changes in grain taste and colour within the same type. Understanding these variations could help conserve and sustain sorghum genetic resources in Burkina Faso.
基金Supported by Guizhou Provincial Science and Technology Projects (QKHJC(2019)1294)Innovation Group Project of Guizhou Provincial Department of Education (QJH-KY-Z[2020]023)+1 种基金Moutai Institute High-level Talents Research Project (MYGCCRC[2022]064)Innovation and Entrepreneurship Training Program for College Students of Moutai Institute (X202214625040)。
文摘In order to find out the effects of soil fluorine on ecological environment and crop safety in the sorghum cultivation base of Renhuai City, Guizhou Province, the content and the occurrence forms of soil fluorine and its influencing factors of Maoba Town were investigated and studied. The results showed that the total fluorine content in the soil of the sorghum base ranged from 668.60 to 2 596.80 mg/kg, with an average of 1 483.25 mg/kg, which was 3.10 and 1.85 times of the national soil background value and the average value of the soil in endemic areas, respectively. The spatial distribution of fluorine in the soil of the study area was uneven, and the fluorine content was in the order of Anliang Village>Yangliu Village>Houba Village>Xiongfeng Village. The soil fluorine contents of various forms varied greatly, and the distribution law was as follows: residual state>water soluble state>organically bound state>exchangeable state>Fe/Mn-F state. The residual fluorine content was the highest, accounting for more than 99% of total fluorine content, while the sum of other four forms was less than 1%, with their respective proportions ranging from 0.10% to 0.30%. The content and proportion of available fluorine in water-soluble and exchangeable states were relatively low, which had limited influence on the surrounding water environment and crop safety. The physicochemical properties of soil, such as pH, organic matter, cation exchange capacity and clay particles had a certain influence on the occurrence forms of soil fluorine, thus changing the availability and migration of soil fluorine, which might endanger the ecological environment safety, food safety and human health.
文摘Participatory varietal selection (PVS) with farmers and Seed Companies was conducted at Maradi research station (07°05'E/13°48'N) and in farmer field to evaluate and select sorghum hybrid varieties for high yield and other important agronomic traits. The experimental design was a randomized complete block with three replications where flowering (days), plant height (cm) and yield (kg) were collected. The analysis of variance showed highly significance among treatments of all traits measured Environment and Genotype by Environment interactions also contributed significantly to the performance of yield components. The highest average grain yield was recorded from hybrids P9511A x ST9007-5-2-1 (4289 kg/ha), NE223A x 90SN1 (3666 kg/ha), NE223A x Sepon 82 (3533 kg/ha) and NE223A x P9405 (3519 kg/ha) across locations. Farmers’ preferences were the panicle size, good seed set, earliness, and seed color. Hence, in a variety selection farmer’s preferences focus more on prioritized yield-related trait. The best varieties ranked by traits of interest were P9511A x ST9007-5-2-1, NE223A x 90SN1, NE223A x P9405 and P9511A x SEPON 82 that performed well under their circumstances. The results showed that farmers’ preferred varieties match with researchers. Therefore, based on objectively measured traits, farmers’ preferences and the agro ecologies of the site, varieties NE223A x 90SN1 and P9511A x ST9007-5-2-1 were found promising for production.
文摘This research aimed at testing the viability of using Sorghum Stalk Ash (SSA) as a partial replacement of lime in the stabilization of red clay soils for road subgrade construction. Red clay soils have been identified as highly expansive soils, which are affected by both climatic conditions and loading patterns. The consideration of both traffic loading patterns and climatic effects on these soils has been taken into account. A penetration test of 2.5 mm has been used on both pure red soils and stabilized soils at 10% and 15% partial replacement of lime with SSA and showed an improvement in the CBR of stabilized red clay soils up to 11.6%. Again, the PI of stabilized soils at 15% partial replacement of lime reduced up to 11.2%. The results obtained on both CBR and PI of these red clay soils are within the recommended values for the effective subgrade required for laying both permanent and flexible pavements. As a result, a recommendation of making use of SSA to lower the quantities of lime and its costs used in the stabilization of highly expansive soils have been tested through this research. However, further research on a more percentage partial replacement of lime to improve the PI of these soils to below 10% while keeping the CBR levels within the road construction regulations is welcomed.
基金supported by the earmarked fund for China Agriculture Research System(CARS-06)
文摘Sorghum is an important source of food, feed and raw material for brewing, and is expected to be a promising bioenergy crop. Sorghum is well known for its strong resistance to abiotic stress and wide adaptability, and salt tolerance is one of its main characteristics. Increasing sorghum planting acreage on saline-alkalien land is one way to effectively use this kind of marginal soil. In this paper, domestic and overseas research on plant tolerance to soil salinity and alkalinity in sorghum, including salt-tolerant genetics and breeding, physiology, cultivation, and identification of tolerant germplasms, are reviewed. Suggestions for further studies on salinity and alkalinity tolerance in sorghum are given, and the prospects for sorghum production in saline-alkalien land are discussed.
