The Sox genes of Misgurnus auguillicaudatus and Paramisgurnus dabryanuswere detected by PCR with two primer sets. The first primer set was specific to theconservative motif of human SRY gene. Four bands of 200, 550, 9...The Sox genes of Misgurnus auguillicaudatus and Paramisgurnus dabryanuswere detected by PCR with two primer sets. The first primer set was specific to theconservative motif of human SRY gene. Four bands of 200, 550, 940 and 1 000 hp andthree bands of 200, 550 and 900 bp were presented in the PCR products of M.Auguillicaudatus and P. Dabryanas, respectively. Results from Southern blottingindicated that the 200 and 550bp bands are positive. This study also used thedegenerate primer within the HMG-box. With the genomic DNA as template, fourbands were observed with the lengths of 220, 550, 700 and 1500 bp in P. Dabryanus; butfour bands of 220, 530, 570 and 1500hp in M. Auguillicaudatus. It is suggested that thelength divergence in PCR products were due to different sizes of nitrons in the HMG boxes. In addition no sex-specific band was found in these two species.展开更多
Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus ...Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.展开更多
A PCR survey for Sox genes in a young tetraploid fish Tor douronensis (Teleostei:Cyprinidae) was per-formed to access the evolutionary fates of important functional genes after genome duplication caused by polyploidiz...A PCR survey for Sox genes in a young tetraploid fish Tor douronensis (Teleostei:Cyprinidae) was per-formed to access the evolutionary fates of important functional genes after genome duplication caused by polyploidization event. Totally 13 Sox genes were obtained in Tor douronensis,which represent SoxB,SoxC and SoxE groups. Phylogenetic analysis of Sox genes in Tor douronensis provided evidence for fish-specific genome duplication,and suggested that Sox19 might be a teleost specific Sox gene member. Sequence analysis revealed most of the nucleotide substitutions between duplicated copies of Sox genes caused by tetraploidization event or their orthologues in other species are silent substitutions. It would appear that the sequences are under purifying selective pressure,strongly suggesting that they repre-sent functional genes and supporting selection against all null allele at either of two duplicated loci of Sox4a,Sox9a and Sox9b. Surprising variations of the intron length and similarities of two duplicated copies of Sox9a and Sox9b,suggest that Tor douronensis might be an allotetraploidy.展开更多
Sox(Sry-related high mobility group box)基因是由众多具有HMG-box保守基序构成的超基因家族,是与位于雄性动物Y染色体上的Sry基因同源的家族基因,在很多动物中都有表达。由于其编码的蛋白质具有结合DNA的能力,因而认为Sox基因家族是...Sox(Sry-related high mobility group box)基因是由众多具有HMG-box保守基序构成的超基因家族,是与位于雄性动物Y染色体上的Sry基因同源的家族基因,在很多动物中都有表达。由于其编码的蛋白质具有结合DNA的能力,因而认为Sox基因家族是一类重要的转录调控因子。在个体发育过程中,Sox基因广泛参与了动物的早期胚胎发育、性别决定和分化、神经系统发育、软骨及多种组织器官的形成,具有重要的生物学功能。主要对Sox家族基因的功能及其研究进展进行简要的综述。展开更多
Four different Sox genes in Amur sturgeon Acipenser schrenckii and five Sox genes in chum salmon Oncorhynchus keta were identified for the first time in this paper, and there was no sexual difference for these genes i...Four different Sox genes in Amur sturgeon Acipenser schrenckii and five Sox genes in chum salmon Oncorhynchus keta were identified for the first time in this paper, and there was no sexual difference for these genes in these two fishes. For Amur sturgeon, the encoded amino acid sequences exhibited 98%, 98%, 98%, 86% of sequence homology with those reported for human SOX HMG box, respectively, and for chum salmon, the encoded amino acid sequences exhibited 93%,98%,98%,98%,96% of sequence homology with those reported for human SOX HMG box, respectively. Two different forms of Sox9 gene in chum salmon were found, and Sox9 contained a 303 bp and 258 bp introns in the HMG box for chum salmon respectively while 572 bp for Amur sturgeon. No clear similarity was observed between the introns in the Sox9 HMG box of different fishes, but the introns occur in the HMG box region between the second and third bases of the codon for arginine at residue 42 in HMG box for the three fishes, and obey the rule of “GT AG”. and the encoded amino acid sequences in the three Sox9 genes HMG box are very conservative in the two fishes (100% identification). Identification of these genes is a potential step in understanding development regulations including sex determination in Amur sturgeon.展开更多
SRY基因(sex-determining region of the Y chromosome)是人类及哺乳动物睾丸决定因子TDF(testisdetermining factor)的最佳候选基因。SRY蛋白含有204个氨基酸,其中1个约79个氨基酸区域即HMG盒(high mobility group box)是SRY基因编码...SRY基因(sex-determining region of the Y chromosome)是人类及哺乳动物睾丸决定因子TDF(testisdetermining factor)的最佳候选基因。SRY蛋白含有204个氨基酸,其中1个约79个氨基酸区域即HMG盒(high mobility group box)是SRY基因编码蛋白质的唯一功能区。由于SRY的发现,人们进而发现了1个庞大的与性别决定有关的SRY盒-Sox(SRY-related HMG box gene)基因家族。锯缘青蟹是我国重要的海洋经济蟹类之一,其性染色体和性别决定机制仍处于进化的早期阶段,在分子水平上探讨其性别决定机制尚不多见。通过采用PCR技术,以特异扩增人SRY基因HMG盒保守区的1对兼并引物,扩增了锯缘青蟹基因组的Sox基因。结果表明锯缘青蟹雌雄个体与人一样,均能扩增出1条大小约为216bp左右的基因片段,显示出该基因在进化上的高度保守性,为探索锯缘青蟹的性别决定机制及Sox基因的进化提供了分子资料。展开更多
文摘The Sox genes of Misgurnus auguillicaudatus and Paramisgurnus dabryanuswere detected by PCR with two primer sets. The first primer set was specific to theconservative motif of human SRY gene. Four bands of 200, 550, 940 and 1 000 hp andthree bands of 200, 550 and 900 bp were presented in the PCR products of M.Auguillicaudatus and P. Dabryanas, respectively. Results from Southern blottingindicated that the 200 and 550bp bands are positive. This study also used thedegenerate primer within the HMG-box. With the genomic DNA as template, fourbands were observed with the lengths of 220, 550, 700 and 1500 bp in P. Dabryanus; butfour bands of 220, 530, 570 and 1500hp in M. Auguillicaudatus. It is suggested that thelength divergence in PCR products were due to different sizes of nitrons in the HMG boxes. In addition no sex-specific band was found in these two species.
文摘Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.
基金the National Natural Science Foundation of China (Grant No. 30530120)
文摘A PCR survey for Sox genes in a young tetraploid fish Tor douronensis (Teleostei:Cyprinidae) was per-formed to access the evolutionary fates of important functional genes after genome duplication caused by polyploidization event. Totally 13 Sox genes were obtained in Tor douronensis,which represent SoxB,SoxC and SoxE groups. Phylogenetic analysis of Sox genes in Tor douronensis provided evidence for fish-specific genome duplication,and suggested that Sox19 might be a teleost specific Sox gene member. Sequence analysis revealed most of the nucleotide substitutions between duplicated copies of Sox genes caused by tetraploidization event or their orthologues in other species are silent substitutions. It would appear that the sequences are under purifying selective pressure,strongly suggesting that they repre-sent functional genes and supporting selection against all null allele at either of two duplicated loci of Sox4a,Sox9a and Sox9b. Surprising variations of the intron length and similarities of two duplicated copies of Sox9a and Sox9b,suggest that Tor douronensis might be an allotetraploidy.
文摘Sox(Sry-related high mobility group box)基因是由众多具有HMG-box保守基序构成的超基因家族,是与位于雄性动物Y染色体上的Sry基因同源的家族基因,在很多动物中都有表达。由于其编码的蛋白质具有结合DNA的能力,因而认为Sox基因家族是一类重要的转录调控因子。在个体发育过程中,Sox基因广泛参与了动物的早期胚胎发育、性别决定和分化、神经系统发育、软骨及多种组织器官的形成,具有重要的生物学功能。主要对Sox家族基因的功能及其研究进展进行简要的综述。
文摘Four different Sox genes in Amur sturgeon Acipenser schrenckii and five Sox genes in chum salmon Oncorhynchus keta were identified for the first time in this paper, and there was no sexual difference for these genes in these two fishes. For Amur sturgeon, the encoded amino acid sequences exhibited 98%, 98%, 98%, 86% of sequence homology with those reported for human SOX HMG box, respectively, and for chum salmon, the encoded amino acid sequences exhibited 93%,98%,98%,98%,96% of sequence homology with those reported for human SOX HMG box, respectively. Two different forms of Sox9 gene in chum salmon were found, and Sox9 contained a 303 bp and 258 bp introns in the HMG box for chum salmon respectively while 572 bp for Amur sturgeon. No clear similarity was observed between the introns in the Sox9 HMG box of different fishes, but the introns occur in the HMG box region between the second and third bases of the codon for arginine at residue 42 in HMG box for the three fishes, and obey the rule of “GT AG”. and the encoded amino acid sequences in the three Sox9 genes HMG box are very conservative in the two fishes (100% identification). Identification of these genes is a potential step in understanding development regulations including sex determination in Amur sturgeon.
基金Funded by the Shanghai Education Committee (No. 1999-51)
文摘SRY基因(sex-determining region of the Y chromosome)是人类及哺乳动物睾丸决定因子TDF(testisdetermining factor)的最佳候选基因。SRY蛋白含有204个氨基酸,其中1个约79个氨基酸区域即HMG盒(high mobility group box)是SRY基因编码蛋白质的唯一功能区。由于SRY的发现,人们进而发现了1个庞大的与性别决定有关的SRY盒-Sox(SRY-related HMG box gene)基因家族。锯缘青蟹是我国重要的海洋经济蟹类之一,其性染色体和性别决定机制仍处于进化的早期阶段,在分子水平上探讨其性别决定机制尚不多见。通过采用PCR技术,以特异扩增人SRY基因HMG盒保守区的1对兼并引物,扩增了锯缘青蟹基因组的Sox基因。结果表明锯缘青蟹雌雄个体与人一样,均能扩增出1条大小约为216bp左右的基因片段,显示出该基因在进化上的高度保守性,为探索锯缘青蟹的性别决定机制及Sox基因的进化提供了分子资料。
