Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomy...Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomyl as the sole carbon or nitrogen source. The optimal temperature and medium pH for its growth and methomyl biodegradation were 30℃ and 7.0, respectively. It was identified as a Paracoccus sp. according to its morphological features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 16S rDNA. Gas chromatography-mass spectrometry （GC-MS） analysis showed that methomyl could be completely transformed to S-methyl-N-hydroxythioacetamidate in 10 h of incubation with the isolate mdw-1.展开更多
The genetic information encoding metabolic pathways for xenobiotic compounds in bacteria often resides on catabolic plasmids.The aim of the present work was to know the location of the genes for degrading 1,2,4-trichl...The genetic information encoding metabolic pathways for xenobiotic compounds in bacteria often resides on catabolic plasmids.The aim of the present work was to know the location of the genes for degrading 1,2,4-trichlorobenzen.In this paper a 1,2,4-trichlorobenzene-degrading strain THSL-1 was isolated from the soil of Tianjin Chemical Plant using 1,2,4-trichlorobenzene as the sole carbon source.The strain was identified as Pseudomonas stutzeri through morphologic survey and 16S rDNA sequence determination.A plasmid was discovered from strain THSL-1 by using the alkali lysis method.When the plasmid was transformed into E.coli.JM109 by the CaCl2 method,the transformant could grow using 1,2,4-trichlorobenzene as the sole carbon source and had the degradation function of 1,2,4-trichlorobenzene.Therefore,it could be deemed that the plasmid carried the degradative genes of 1,2,4-trichlorobenzene.The average size of the plasmid was finally determined to be 40.2 Kb using selectively three kinds of restricted inscribed enzymes(HindIII,BamHI,and XholI)for single cutting and double cutting the plasmid pTHSL-1,respectively.展开更多
基金Project supported by the National Natural Science Foundation of China(No.30600016)the National High Technology Research and Development Program of China(863 Program)(No.2007AA10Z405)+2 种基金the National Program for Sci-Tech Basic Platform Construction of China(No.2005DKA21201-2)the Program for Science and Technology Development ofJiangsu Province,China(No.BE2008669)the Program for Integration of Production,Education,and Research of Guangdong Province and Ministry of Education,China(No.2006090204007)
文摘Methomyl, an extremely toxic pesticide, is widely used in agriculture. A strain named mdw-1 capable of degrading methomyl rapidly was successfully isolated from activated sludge in this study. It could utilize methomyl as the sole carbon or nitrogen source. The optimal temperature and medium pH for its growth and methomyl biodegradation were 30℃ and 7.0, respectively. It was identified as a Paracoccus sp. according to its morphological features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 16S rDNA. Gas chromatography-mass spectrometry （GC-MS） analysis showed that methomyl could be completely transformed to S-methyl-N-hydroxythioacetamidate in 10 h of incubation with the isolate mdw-1.
基金This work was supported by the National High Technology Research and Development Program of China(863 Program)(Grant Nos.2002AA601170 and 2002A A601150).
文摘The genetic information encoding metabolic pathways for xenobiotic compounds in bacteria often resides on catabolic plasmids.The aim of the present work was to know the location of the genes for degrading 1,2,4-trichlorobenzen.In this paper a 1,2,4-trichlorobenzene-degrading strain THSL-1 was isolated from the soil of Tianjin Chemical Plant using 1,2,4-trichlorobenzene as the sole carbon source.The strain was identified as Pseudomonas stutzeri through morphologic survey and 16S rDNA sequence determination.A plasmid was discovered from strain THSL-1 by using the alkali lysis method.When the plasmid was transformed into E.coli.JM109 by the CaCl2 method,the transformant could grow using 1,2,4-trichlorobenzene as the sole carbon source and had the degradation function of 1,2,4-trichlorobenzene.Therefore,it could be deemed that the plasmid carried the degradative genes of 1,2,4-trichlorobenzene.The average size of the plasmid was finally determined to be 40.2 Kb using selectively three kinds of restricted inscribed enzymes(HindIII,BamHI,and XholI)for single cutting and double cutting the plasmid pTHSL-1,respectively.
