VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in St...VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is limited.In this study,we compared the characteristics that could affect the viability of VIGS in S.japonicus,including the acetosyringone(AS)concentration,the Agrobacterium’s optical density and the inoculation method.The stable reference genes of S.japonicus were selected to validate the gene’s knockdown by quantitative PCR.As a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200μmol·L^(-1)and OD600of 0.5,and friction-osmosis with AS concentration of 200μmol·L^(-1)and OD600of 1.0,which silencing efficiency was 83.33%and 74.19%,respectively.The successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in S.japonicus to identify unknown gene functions.展开更多
[Objectives]To explore the response characteristics of chlorophyll and photosynthesis in Styrax tonkinensis under different concentrations of copper and cadmium stress and analyze the internal mechanism of the effects...[Objectives]To explore the response characteristics of chlorophyll and photosynthesis in Styrax tonkinensis under different concentrations of copper and cadmium stress and analyze the internal mechanism of the effects of copper and cadmium pollution stress on photosynthesis.[Methods]Taking seedlings of S.tonkinensis as the research object,the response characteristics of chlorophyll and photosynthesis of S.tonkinensis were compared under different concentrations of copper and cadmium.[Results]With the increase of copper and cadmium treatment concentrations,the chlorophyll content first rose and then fell.With the increase of cadmium concentration,the net photosynthetic rate of S.tonkinensis plants increased from decrease to increase,and the stomatal conductance,transpiration rate and intercellular CO_(2) concentration all increased.[Conclusions]Under the stress of different concentrations of cadmium,the photosynthetic characteristics of S.tonkinensis were not obviously inhibited.Copper stress has inhibitory effect on photosynthesis of S.tonkinensis.展开更多
Styrax is also called“emperor’s cream”and“consciousness-regaining spice.”The alias“emperor’s cream”shows its rarity and preciousness with a strong aroma,and another name of“consciousness-regaining spice”impl...Styrax is also called“emperor’s cream”and“consciousness-regaining spice.”The alias“emperor’s cream”shows its rarity and preciousness with a strong aroma,and another name of“consciousness-regaining spice”implies its functions of active brain and regaining consciousness,dispelling filth with aroma,and relieving pain.The name of“Styrax”displays its complex productive progress and place of origin.With thick quality,heavyweight,and fragrant smell,Styrax gets further application in beauty,skin care,clothes fumigating,and health keeping after introduced into the Central Plains(central China)from the Western Regions.The name of Styrax not only embodies the mellow appeal of spice culture in the Western Regions but also suggests the history of relationship between foreign culture and traditional Chinese medicine.Furthermore,it indicates the broad mind of“all rivers running into sea”and inclusiveness of Chinese culture.展开更多
Atoms in most organic molecules are often carbon,oxygen,nitrogen,sulfur,halogens,etc. Based on the three-dimensional structure of a molecule,a molecular structural characterization(MSC) method called improved molecula...Atoms in most organic molecules are often carbon,oxygen,nitrogen,sulfur,halogens,etc. Based on the three-dimensional structure of a molecule,a molecular structural characterization(MSC) method called improved molecular electronegativity-distance vector(I-MEDV) was developed. It was used to describe the structures of 37 compounds of styrax japonicus sieb flowers. Through multiple linear regression(MLR),a QSRR model was built up. The correlation coefficient(R1) of the model was 0.980. Then,4 vectors were selected to build another model through the method of stepwise multiple regression(SMR) ,and the correlation coefficient(R2) of the model was 0.975. Moreover,all the two models were evaluated by performing the crossvalidation with the leave-one-out(LOO) procedure and the correlation coefficients(Rcv) were 0.948 and 0.968,respectively. The results show that the I-MEDV could successfully describe the structures of organic compounds. The stability and predictability of the models were good.展开更多
基金supported by the Subject of Key R&D Plan of Shandong Province(Major Scientific and Technological Innovation Project)“Mining and Accurate Identification of Forest Tree Germplasm Resources”(Grant Nos.2021LZGC02303)Science&Technology Specific Projects in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta(Grant No.022SZX16)。
文摘VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is limited.In this study,we compared the characteristics that could affect the viability of VIGS in S.japonicus,including the acetosyringone(AS)concentration,the Agrobacterium’s optical density and the inoculation method.The stable reference genes of S.japonicus were selected to validate the gene’s knockdown by quantitative PCR.As a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200μmol·L^(-1)and OD600of 0.5,and friction-osmosis with AS concentration of 200μmol·L^(-1)and OD600of 1.0,which silencing efficiency was 83.33%and 74.19%,respectively.The successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in S.japonicus to identify unknown gene functions.
基金Young Talents Training Project of Jiangxi Academy of Forestry(2015520801).
文摘[Objectives]To explore the response characteristics of chlorophyll and photosynthesis in Styrax tonkinensis under different concentrations of copper and cadmium stress and analyze the internal mechanism of the effects of copper and cadmium pollution stress on photosynthesis.[Methods]Taking seedlings of S.tonkinensis as the research object,the response characteristics of chlorophyll and photosynthesis of S.tonkinensis were compared under different concentrations of copper and cadmium.[Results]With the increase of copper and cadmium treatment concentrations,the chlorophyll content first rose and then fell.With the increase of cadmium concentration,the net photosynthetic rate of S.tonkinensis plants increased from decrease to increase,and the stomatal conductance,transpiration rate and intercellular CO_(2) concentration all increased.[Conclusions]Under the stress of different concentrations of cadmium,the photosynthetic characteristics of S.tonkinensis were not obviously inhibited.Copper stress has inhibitory effect on photosynthesis of S.tonkinensis.
文摘Styrax is also called“emperor’s cream”and“consciousness-regaining spice.”The alias“emperor’s cream”shows its rarity and preciousness with a strong aroma,and another name of“consciousness-regaining spice”implies its functions of active brain and regaining consciousness,dispelling filth with aroma,and relieving pain.The name of“Styrax”displays its complex productive progress and place of origin.With thick quality,heavyweight,and fragrant smell,Styrax gets further application in beauty,skin care,clothes fumigating,and health keeping after introduced into the Central Plains(central China)from the Western Regions.The name of Styrax not only embodies the mellow appeal of spice culture in the Western Regions but also suggests the history of relationship between foreign culture and traditional Chinese medicine.Furthermore,it indicates the broad mind of“all rivers running into sea”and inclusiveness of Chinese culture.
基金supported by the Youth Foundation of Education Bureau,Sichuan Province (09ZB036)Technology Bureau,Sichuan Province (2006j13-141)
文摘Atoms in most organic molecules are often carbon,oxygen,nitrogen,sulfur,halogens,etc. Based on the three-dimensional structure of a molecule,a molecular structural characterization(MSC) method called improved molecular electronegativity-distance vector(I-MEDV) was developed. It was used to describe the structures of 37 compounds of styrax japonicus sieb flowers. Through multiple linear regression(MLR),a QSRR model was built up. The correlation coefficient(R1) of the model was 0.980. Then,4 vectors were selected to build another model through the method of stepwise multiple regression(SMR) ,and the correlation coefficient(R2) of the model was 0.975. Moreover,all the two models were evaluated by performing the crossvalidation with the leave-one-out(LOO) procedure and the correlation coefficients(Rcv) were 0.948 and 0.968,respectively. The results show that the I-MEDV could successfully describe the structures of organic compounds. The stability and predictability of the models were good.
