目的评估Solumbra技术和SWIM技术治疗急性缺血性脑卒中(acute ischemic stroke,AIS)的有效性与安全性。方法回顾性分析191例AIS病例资料,根据手术方式分为Solumbra组(n=97)和SWIM组(n=94)。应用倾向性评分匹配法(propensity score match...目的评估Solumbra技术和SWIM技术治疗急性缺血性脑卒中(acute ischemic stroke,AIS)的有效性与安全性。方法回顾性分析191例AIS病例资料,根据手术方式分为Solumbra组(n=97)和SWIM组(n=94)。应用倾向性评分匹配法(propensity score matching,PSM)将两组患者按1∶1配比。对比匹配后两组患者临床相关指标、术后不同时间点美国国立卫生院卒中量表(the National Institutes of Health Stroke Scale,NIHSS)评分、侧支循环改善情况及并发症发生率。结果经PSM匹配,有50对患者匹配成功。Solumbra组患者取栓>2次占比与住院时间均高于SWIM组,预后良好占比低于SWIM组(P<0.05)。Solumbra组患者术后7d及术后2周NIHSS评分均低于SWIM组(P<0.05)。并发症发生率Solumbra组为20.00%,SWIM组为38.00%,Solumbra组低于SWIM组(P<0.05)。结论Solumbra技术和SWIM技术取栓治疗各有优势,临床可充分结合患者实际病情,针对性选择取栓方式。展开更多
The three-dimensional lattice Boltzmann method(LBM)is used to simulate the motion of a spherical squirmer in a square tube,and the steady motion velocity of a squirmer with different Reynolds numbers(Re,ranging from 0...The three-dimensional lattice Boltzmann method(LBM)is used to simulate the motion of a spherical squirmer in a square tube,and the steady motion velocity of a squirmer with different Reynolds numbers(Re,ranging from 0.1 to 2)and swimming types is investigated and analyzed to better understand the swimming characteristics of microorganisms in different environments.First,as the Reynolds number increases,the effect of the inertial forces becomes significant,disrupting the squirmer's ability to maintain its theoretical velocity.Specifically,as the Reynolds number increases,the structure of the flow field around the squirmer changes,affecting its velocity of motion.Notably,the swimming velocity of the squirmer exhibits a quadratic relationship with the type of swimming and the Reynolds number.Second,the narrow tube exerts a significant inhibitory effect on the squirmer motion.In addition,although chirality does not directly affect the swimming velocity of the squirmer,it can indirectly affect the velocity by changing its motion mode.展开更多
This study was designed to determine the effect of different sperm preparation treatments on yak sperm quality and in vitro embryo development.Frozen-thawed semen samples were treated using swim-up or percoll gradient...This study was designed to determine the effect of different sperm preparation treatments on yak sperm quality and in vitro embryo development.Frozen-thawed semen samples were treated using swim-up or percoll gradient centrifugation methods.Sperm concentration,progressive motility,recovery of motile sperm,membrane integrity,acrosome and chromatin integrity were scored and compared in recovered samples and controls.In addition,the effects of two sperm separation treatments on embryos capable of cleavage and in vitro development to the blastocyst stage were evaluated.Swim-up separated sperm showed a higher motility,while the concentration of spermatozoa recovered and percent recovery of motile sperm were higher with percoll gradient centrifugation separation.According to the optical and electron microscopies,swim-up produced higher percentage of sperm with intact plasma membrane and acrosome than percoll gradient centrifugation separation.However,there was no difference in the percentage of sperm with intact chromatin between two treatment groups.Cell numbers in the blastocysts of two groups were not different.The blastocyst rate was similar in both groups,whereas cleavage rate was higher when swim-up was used.展开更多
Complete blood counts and blood chemistry in four Siberian husky puppies diagnosed with swimming puppy syndrome were compared with four healthy Siberian husky puppies. Only serum creatine kinase in affected puppies wa...Complete blood counts and blood chemistry in four Siberian husky puppies diagnosed with swimming puppy syndrome were compared with four healthy Siberian husky puppies. Only serum creatine kinase in affected puppies was significantly (P < 0.01) higher than in normal puppies. Although serum creatine kinase level cannot be used for diagnosis of this disease, it could serve as a tool for prognosis of the disease after treatment.展开更多
Background:Vascular cognitive impairment caused by chronic cerebral hypoperfusion(CCH)has become a hot issue worldwide.Aerobic exercise positively contributes to the preservation or restoration of cognitive abilities;...Background:Vascular cognitive impairment caused by chronic cerebral hypoperfusion(CCH)has become a hot issue worldwide.Aerobic exercise positively contributes to the preservation or restoration of cognitive abilities;however,the specific mechanism has remained inconclusive.And recent studies found that neurogranin(Ng)is a potential biomarker for cognitive impairment.This study aims to investigate the underlying role of Ng in swimming training to improve cognitive impairment.Methods:To test this hypothesis,the clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9(Cas9)system was utilized to construct a strain of Ng conditional knockout(Ng cKO)mice,and bilateral common carotid artery stenosis(BCAS)surgery was performed to prepare the model.In Experiment 1,2-month-old male and female transgenic mice were divided into a control group(wild-type littermate,n=9)and a Ng cKO group(n=9).Then,2-month-old male and female C57BL/6 mice were divided into a sham group(C57BL/6,n=12)and a BCAS group(n=12).In Experiment 2,2-month-old male and female mice were divided into a sham group(wild-type littermate,n=12),BCAS group(n=12),swim group(n=12),BCAS+Ng cKO group(n=12),and swim+Ng cKO group(n=12).Then,7 days after BCAS,mice were given swimming training for 5 weeks(1 week for adaptation and 4 weeks for training,5 days a week,60 min a day).After intervention,laser speckle was used to detect cerebral blood perfusion in the mice,and the T maze and Morris water maze were adopted to test their spatial memory.Furthermore,electrophysiology and Western blotting were conducted to record long-term potential and observe the expressions of Ca^(2+)pathway-related proteins,respectively.Immunohistochemistry was applied to analyze the expression of relevant markers in neuronal damage,inflammation,and white matter injury.Results:The figures showed that spatial memory impairment was detected in Ng cKO mice,and a sharp decline of cerebral blood flow and an impairment of progressive spatial memory were observed in BCAS mice.Regular swimming training improved the spatial memory impairment of BCAS mice.This was achieved by preventing long-term potential damage and reversing the decline of Ca^(2+)signal transduction pathway-related proteins.At the same time,the results suggested that swimming also led to improvements in neuronal death,inflammation,and white matter injury induced by CCH.Further study adopted the use of Ng cKO transgenic mice,and the results indicated that the positive effects of swimming training on cognitive impairments,synaptic plasticity,and related pathological changes caused by CCH could be abolished by the knockout of Ng.Conclusion:Swimming training can mediate the expression of Ng to enhance hippocampal synaptic plasticity and improve related pathological changes induced by CCH,thereby ameliorating the spatial memory impairment of vascular cognitive impairment.展开更多
Physical exe rcise effectively alleviates chronic pain associated with complex regional pain syndrome type-Ⅰ.However,the mechanism of exe rcise-induced analgesia has not been clarified.Recent studies have shown that ...Physical exe rcise effectively alleviates chronic pain associated with complex regional pain syndrome type-Ⅰ.However,the mechanism of exe rcise-induced analgesia has not been clarified.Recent studies have shown that the specialized pro-resolving lipid mediator resolvin E1 promotes relief of pathologic pain by binding to chemerin receptor 23 in the nervous system.However,whether the resolvin E1-chemerin receptor 23 axis is involved in exercise-induced analgesia in complex regional pain syndrome type-Ⅰ has not been demonstrated.In the present study,a mouse model of chronic post-ischemia pain was established to mimic complex regional pain syndrome type-Ⅰ and subjected to an intervention involving swimming at different intensities.Chronic pain was reduced only in mice that engaged in high-intensity swimming.The resolvin E1-chemerin receptor 23 axis was clearly downregulated in the spinal cord of mice with chronic pain,while high-intensity swimming restored expression of resolvin E1 and chemerin receptor 23.Finally,shRNA-mediated silencing of chemerin receptor 23in the spinal cord reve rsed the analgesic effect of high-intensity swimming exercise on chronic post-ischemic pain and the anti-inflammato ry pola rization of microglia in the dorsal horn of the spinal cord.These findings suggest that high-intensity swimming can decrease chronic pain via the endogenous resolvin E1-chemerin receptor 23 axis in the spinal cord.展开更多
The blue swimming crab,Portunus pelagicus(Linnaeus,1758),is one of the most economically important crustaceans in Thailand.In order to gather more in-country data on its biological aspects,a comprehensive observation ...The blue swimming crab,Portunus pelagicus(Linnaeus,1758),is one of the most economically important crustaceans in Thailand.In order to gather more in-country data on its biological aspects,a comprehensive observation of the annual reproductive cycle of female P.pelagicus,which were gathered each month from Kung Krabaen Bay,Thailand,from October 2017 to September 2018 was undertaken.The ovarian structure,gonadosomatic index(GSI)and histological description of P.pelagicus were observed during this time.It was revealed that six distinct stages—I(immature stage),II(early maturing stage),III(late maturing stage),IV(early matured stage),V(middle matured stage)and VI(late matured stage)—occur during the annual reproductive cycle of the females.Moreover,the percentage of the oogenic proportion of P.pelagicus appeared continuously throughout the year.The ovarian development was exclusively found in stage VI,during October 2017 to December 2017,with the highest peak in November 2017(39.96%)in relation to the high GSI.From October 2017 to February 2018,stage I was also observed,especially during October 2017(49.5%),implying its important involvement in supporting oogenic production.It would be suggested that the successive spawning season of the P.pelagicus population clearly occurred during October-December each year.Therefore,this would be the best time to support hatchery seed production,during which P.pelagicus collection should be avoided.展开更多
Hydrodynamic force is an important factor that affects the performance of underwater vehicle.Adapting to the current underwater environment by changing its shape is an important feature of underwater snake-like robots...Hydrodynamic force is an important factor that affects the performance of underwater vehicle.Adapting to the current underwater environment by changing its shape is an important feature of underwater snake-like robots(USLR).An experiment was implemented to verify the swimming along the straight line of USLR.A simulation platform is also established for the analysis of the swimming of USLR.To figure out adaptive swimming of USLR to different underwater environments,the relationships between CPG parameters and maximum swimming speed have been discussed,and the switching between different swimming modes has been implemented.展开更多
目的总结颅内支撑导管辅助Solitaire支架取栓+吸栓技术(Solitaire FR with intracranial support catheter for mechanical thrombectimy,SWIM)治疗大血管闭塞性脑梗死的围手术期护理要点。方法对80例急性大血管闭塞性脑梗死患者采用SWI...目的总结颅内支撑导管辅助Solitaire支架取栓+吸栓技术(Solitaire FR with intracranial support catheter for mechanical thrombectimy,SWIM)治疗大血管闭塞性脑梗死的围手术期护理要点。方法对80例急性大血管闭塞性脑梗死患者采用SWIM技术进行取栓和吸栓相结合开通血管,加强围手术期护理管理。结果 80例患者中,75例血管成功再通,5例血管再通不佳,术中医护配合良好。结论快速有效地完善术前准备、密切观察病情、熟练的术中护理配合、仔细认真的术后护理有利于血管再通治疗顺利完成,改善患者预后。展开更多
文摘目的评估Solumbra技术和SWIM技术治疗急性缺血性脑卒中(acute ischemic stroke,AIS)的有效性与安全性。方法回顾性分析191例AIS病例资料,根据手术方式分为Solumbra组(n=97)和SWIM组(n=94)。应用倾向性评分匹配法(propensity score matching,PSM)将两组患者按1∶1配比。对比匹配后两组患者临床相关指标、术后不同时间点美国国立卫生院卒中量表(the National Institutes of Health Stroke Scale,NIHSS)评分、侧支循环改善情况及并发症发生率。结果经PSM匹配,有50对患者匹配成功。Solumbra组患者取栓>2次占比与住院时间均高于SWIM组,预后良好占比低于SWIM组(P<0.05)。Solumbra组患者术后7d及术后2周NIHSS评分均低于SWIM组(P<0.05)。并发症发生率Solumbra组为20.00%,SWIM组为38.00%,Solumbra组低于SWIM组(P<0.05)。结论Solumbra技术和SWIM技术取栓治疗各有优势,临床可充分结合患者实际病情,针对性选择取栓方式。
基金Project supported by the National Natural Science Foundation of China(Nos.12132015 and 12372251)the Fundamental Research Funds for the Provincial Universities of Zhejiang of China(No.2023YW69)。
文摘The three-dimensional lattice Boltzmann method(LBM)is used to simulate the motion of a spherical squirmer in a square tube,and the steady motion velocity of a squirmer with different Reynolds numbers(Re,ranging from 0.1 to 2)and swimming types is investigated and analyzed to better understand the swimming characteristics of microorganisms in different environments.First,as the Reynolds number increases,the effect of the inertial forces becomes significant,disrupting the squirmer's ability to maintain its theoretical velocity.Specifically,as the Reynolds number increases,the structure of the flow field around the squirmer changes,affecting its velocity of motion.Notably,the swimming velocity of the squirmer exhibits a quadratic relationship with the type of swimming and the Reynolds number.Second,the narrow tube exerts a significant inhibitory effect on the squirmer motion.In addition,although chirality does not directly affect the swimming velocity of the squirmer,it can indirectly affect the velocity by changing its motion mode.
基金supported by the National Natural Science Foundation of China(30871903)the Fundamental Research Funds for the Gansu Universities,China(2011)
文摘This study was designed to determine the effect of different sperm preparation treatments on yak sperm quality and in vitro embryo development.Frozen-thawed semen samples were treated using swim-up or percoll gradient centrifugation methods.Sperm concentration,progressive motility,recovery of motile sperm,membrane integrity,acrosome and chromatin integrity were scored and compared in recovered samples and controls.In addition,the effects of two sperm separation treatments on embryos capable of cleavage and in vitro development to the blastocyst stage were evaluated.Swim-up separated sperm showed a higher motility,while the concentration of spermatozoa recovered and percent recovery of motile sperm were higher with percoll gradient centrifugation separation.According to the optical and electron microscopies,swim-up produced higher percentage of sperm with intact plasma membrane and acrosome than percoll gradient centrifugation separation.However,there was no difference in the percentage of sperm with intact chromatin between two treatment groups.Cell numbers in the blastocysts of two groups were not different.The blastocyst rate was similar in both groups,whereas cleavage rate was higher when swim-up was used.
文摘Complete blood counts and blood chemistry in four Siberian husky puppies diagnosed with swimming puppy syndrome were compared with four healthy Siberian husky puppies. Only serum creatine kinase in affected puppies was significantly (P < 0.01) higher than in normal puppies. Although serum creatine kinase level cannot be used for diagnosis of this disease, it could serve as a tool for prognosis of the disease after treatment.
基金Supported by the Youth Top Talent Project of Fujian Province,China“Young Eagle Project”(No.2901-750102003)。
文摘Background:Vascular cognitive impairment caused by chronic cerebral hypoperfusion(CCH)has become a hot issue worldwide.Aerobic exercise positively contributes to the preservation or restoration of cognitive abilities;however,the specific mechanism has remained inconclusive.And recent studies found that neurogranin(Ng)is a potential biomarker for cognitive impairment.This study aims to investigate the underlying role of Ng in swimming training to improve cognitive impairment.Methods:To test this hypothesis,the clustered regularly interspaced short palindromic repeats(CRISPR)-associated protein 9(Cas9)system was utilized to construct a strain of Ng conditional knockout(Ng cKO)mice,and bilateral common carotid artery stenosis(BCAS)surgery was performed to prepare the model.In Experiment 1,2-month-old male and female transgenic mice were divided into a control group(wild-type littermate,n=9)and a Ng cKO group(n=9).Then,2-month-old male and female C57BL/6 mice were divided into a sham group(C57BL/6,n=12)and a BCAS group(n=12).In Experiment 2,2-month-old male and female mice were divided into a sham group(wild-type littermate,n=12),BCAS group(n=12),swim group(n=12),BCAS+Ng cKO group(n=12),and swim+Ng cKO group(n=12).Then,7 days after BCAS,mice were given swimming training for 5 weeks(1 week for adaptation and 4 weeks for training,5 days a week,60 min a day).After intervention,laser speckle was used to detect cerebral blood perfusion in the mice,and the T maze and Morris water maze were adopted to test their spatial memory.Furthermore,electrophysiology and Western blotting were conducted to record long-term potential and observe the expressions of Ca^(2+)pathway-related proteins,respectively.Immunohistochemistry was applied to analyze the expression of relevant markers in neuronal damage,inflammation,and white matter injury.Results:The figures showed that spatial memory impairment was detected in Ng cKO mice,and a sharp decline of cerebral blood flow and an impairment of progressive spatial memory were observed in BCAS mice.Regular swimming training improved the spatial memory impairment of BCAS mice.This was achieved by preventing long-term potential damage and reversing the decline of Ca^(2+)signal transduction pathway-related proteins.At the same time,the results suggested that swimming also led to improvements in neuronal death,inflammation,and white matter injury induced by CCH.Further study adopted the use of Ng cKO transgenic mice,and the results indicated that the positive effects of swimming training on cognitive impairments,synaptic plasticity,and related pathological changes caused by CCH could be abolished by the knockout of Ng.Conclusion:Swimming training can mediate the expression of Ng to enhance hippocampal synaptic plasticity and improve related pathological changes induced by CCH,thereby ameliorating the spatial memory impairment of vascular cognitive impairment.
基金National Key R&D Program of China,Nos.2019YFA0110300 (to LZ),2021YFA1201400 (to LZ)Natural Science Foundation of Shanghai,No.21ZR1468600 (to LZ)Open Fund of the Key Laboratory of Cellular Physiology (Shanxi Medical University),Ministry of Education,No.KLMEC/SXMU-201910 (to XJ)。
文摘Physical exe rcise effectively alleviates chronic pain associated with complex regional pain syndrome type-Ⅰ.However,the mechanism of exe rcise-induced analgesia has not been clarified.Recent studies have shown that the specialized pro-resolving lipid mediator resolvin E1 promotes relief of pathologic pain by binding to chemerin receptor 23 in the nervous system.However,whether the resolvin E1-chemerin receptor 23 axis is involved in exercise-induced analgesia in complex regional pain syndrome type-Ⅰ has not been demonstrated.In the present study,a mouse model of chronic post-ischemia pain was established to mimic complex regional pain syndrome type-Ⅰ and subjected to an intervention involving swimming at different intensities.Chronic pain was reduced only in mice that engaged in high-intensity swimming.The resolvin E1-chemerin receptor 23 axis was clearly downregulated in the spinal cord of mice with chronic pain,while high-intensity swimming restored expression of resolvin E1 and chemerin receptor 23.Finally,shRNA-mediated silencing of chemerin receptor 23in the spinal cord reve rsed the analgesic effect of high-intensity swimming exercise on chronic post-ischemic pain and the anti-inflammato ry pola rization of microglia in the dorsal horn of the spinal cord.These findings suggest that high-intensity swimming can decrease chronic pain via the endogenous resolvin E1-chemerin receptor 23 axis in the spinal cord.
文摘The blue swimming crab,Portunus pelagicus(Linnaeus,1758),is one of the most economically important crustaceans in Thailand.In order to gather more in-country data on its biological aspects,a comprehensive observation of the annual reproductive cycle of female P.pelagicus,which were gathered each month from Kung Krabaen Bay,Thailand,from October 2017 to September 2018 was undertaken.The ovarian structure,gonadosomatic index(GSI)and histological description of P.pelagicus were observed during this time.It was revealed that six distinct stages—I(immature stage),II(early maturing stage),III(late maturing stage),IV(early matured stage),V(middle matured stage)and VI(late matured stage)—occur during the annual reproductive cycle of the females.Moreover,the percentage of the oogenic proportion of P.pelagicus appeared continuously throughout the year.The ovarian development was exclusively found in stage VI,during October 2017 to December 2017,with the highest peak in November 2017(39.96%)in relation to the high GSI.From October 2017 to February 2018,stage I was also observed,especially during October 2017(49.5%),implying its important involvement in supporting oogenic production.It would be suggested that the successive spawning season of the P.pelagicus population clearly occurred during October-December each year.Therefore,this would be the best time to support hatchery seed production,during which P.pelagicus collection should be avoided.
文摘Hydrodynamic force is an important factor that affects the performance of underwater vehicle.Adapting to the current underwater environment by changing its shape is an important feature of underwater snake-like robots(USLR).An experiment was implemented to verify the swimming along the straight line of USLR.A simulation platform is also established for the analysis of the swimming of USLR.To figure out adaptive swimming of USLR to different underwater environments,the relationships between CPG parameters and maximum swimming speed have been discussed,and the switching between different swimming modes has been implemented.
文摘目的总结颅内支撑导管辅助Solitaire支架取栓+吸栓技术(Solitaire FR with intracranial support catheter for mechanical thrombectimy,SWIM)治疗大血管闭塞性脑梗死的围手术期护理要点。方法对80例急性大血管闭塞性脑梗死患者采用SWIM技术进行取栓和吸栓相结合开通血管,加强围手术期护理管理。结果 80例患者中,75例血管成功再通,5例血管再通不佳,术中医护配合良好。结论快速有效地完善术前准备、密切观察病情、熟练的术中护理配合、仔细认真的术后护理有利于血管再通治疗顺利完成,改善患者预后。
