MicroRNAs（miRNAs） are a population of highly conserved specific small ribo-regulators that negatively regulate gene expressions in both plants and animals.They play a key role in post-transcriptional gene regulation...MicroRNAs（miRNAs） are a population of highly conserved specific small ribo-regulators that negatively regulate gene expressions in both plants and animals.They play a key role in post-transcriptional gene regulation by destabilizing the target gene transcripts or blocking protein translation from them.Interestingly,these negative regulators are largely compromised by an upstream layer of negative regulators ＂target mimics＂ found in plants or ＂endogenous competing RNAs＂ revealed recently in animals.These endogenous regulatory mechanisms of ＂double negatives making a positive＂ have now been developed into a key strategy in the study of small RNA functions. This review presents some reflections on the long journey to the short tandem target mimic（STTM） for selective destruction/blockage of specific miRNAs in plants and animals,and the potential applications of STTM are discussed.展开更多
OBJECTIVE To observe the anti-aging effects of SOD mimic AEOL^(-1)0150 in antisenescence accelerated mouse resistant 1(SAMR1)strain.METHODS The lifespan of SAMR1 mice were observed by subcutaneous injection AEOL^(-1)0...OBJECTIVE To observe the anti-aging effects of SOD mimic AEOL^(-1)0150 in antisenescence accelerated mouse resistant 1(SAMR1)strain.METHODS The lifespan of SAMR1 mice were observed by subcutaneous injection AEOL^(-1)0150 2 mg·kg-1once a week.Morris water maze,new object recognition,nesting and forced swimming were used to observe the behavioral changes of animals.Lymphocyte subgroups and ROS were measured by Flow cytometry.The cytokines levels were determined by Luminex method.The number of DCX+neurons in brain tissue was observed by immunofluorescence.RESULTS The results showed that AEOL^(-1)0150 could prolong the mean lifespan of SAMR1 mice,but it had no obvious effect on maximal lifespan.What′s more,AEOL^(-1)0150 could significantly improve the spatial learning memory of aged mice,but it could not increase the number of DCX+neurons in the hypothalamic MBH and hippocampal DG regions.Then,we observed the effects of AEOL^(-1)0150 on peripheral blood lymphocyte subgroups and cytokines.We found that AEOL^(-1)0150significantly modulated the lymphocyte subgroups and cytokine release.Especially,AEOL^(-1)0150 can dose-dependently inhibit plasma levels of SASP related inflammatory cytokines TNF-αand IL^(-1)7.CONCLUSION The results indicate that AEOL^(-1)0150 has anti-aging effects,and the effects are closely related to modulating immunity and inhibiting SASP production.展开更多
小串联模拟靶标(Short tandem target mimic,STTM)技术是一种新开发的miRNA功能研究方法。Tae-miR9677作为一种新发现的在小麦穗部特异性高表达的miRNA,其功能至今未知。为了进一步探索Tae-miR9677的功能,构建了Ubiqutin(UBI)启动子启动...小串联模拟靶标(Short tandem target mimic,STTM)技术是一种新开发的miRNA功能研究方法。Tae-miR9677作为一种新发现的在小麦穗部特异性高表达的miRNA,其功能至今未知。为了进一步探索Tae-miR9677的功能,构建了Ubiqutin(UBI)启动子启动的Tae-miR9677 STTM过表达载体,并通过基因枪介导法对小麦品种绵阳19幼胚愈伤组织进行转化。结果表明,3 683个愈伤组织经过PPT(Phosphinothricin)筛选,最终分化获得42株再生植株;利用特异性引物进行PCR检测,鉴定出8株T0代阳性植株。展开更多
This study examined the expressions of miR-22 and miR-135a in rats with acute edematous pancreatitis （AEP） and their target genes in order to shed light on the involvement of miR-22 and miR-135a in the pathogenesis ...This study examined the expressions of miR-22 and miR-135a in rats with acute edematous pancreatitis （AEP） and their target genes in order to shed light on the involvement of miR-22 and miR-135a in the pathogenesis of acute pancreatitis （AP）. The in vivo model of AEP was established by introperitoneal injection of L-arginine （150 mg/kg） in rats. The miRNA microarray analysis was used to detect the differential expression of miRNAs in pancreatic tissue in AEP and normal rats. The in vitro AEP model was established by inducing the rat pancreatic acinar cell line （AR42J） with 50 ng/mL re- combinant rat TNF-ct. Real-time quantitative RT-PCR was employed to detect the expression of miR-22 and miR-135a in AR42J cells. Lentiviruses carrying the miRNA mimic and anti-miRNA oligonucleotide （AMO） of miR-22 and miR-135a were transfected into the AR42J cells. The AR42J cells transfected with vehicle served as control. Western blotting was used to measure the expression of activated cas- pase3 and flow cytometry analysis to detect the apoptosis of AR42J cells. Targets of miR-22 and miR-135a were predicted by using TargetScan, miRanda, and TarBase. Luciferase reporter assay and quantitative real-time RT-PCR were performed to confirm whether ErbB3 and Ptk2 were the target gene of miR-22 and miR-135a, respectively. The results showed that the expression levels of miR-22 and miR-135a were obviously increased in AEP group compared with the control group in in-vivo and in-vitro models. The expression levels of miR-22 and miR-135a were elevated conspicuously and the expression levels of their target genes were reduced significantly in AR42J cells transfected with Ienti- viruses carrying the miRNA mimic. The apoptosis rate was much higher in the TNF-ct-induced cells than in non-treated cells. The AR42J cells transfected with miRNA AMOs expressed lower level of miR-22 and miR-135a and had lower apoptosis rate, but the expression levels of ErbB3 and Ptk2 were increased obviously. It was concluded that the expression levels of miR-22 and miR-135a were elevated in AEP. Up-regulating the expression of miR-22 and miR-135a may promote the apoptosis of pancreatic acinar cells by repressing ErbB3 and Ptk2 expression in AEP.展开更多
Long noncoding RNAs （IncRNAs） participate in the regulation of multiple biological processes via diverse manners, one of which is functioning as endogenous target mimics （eTMs） to modulate microRNAs （miRNAs） by ...Long noncoding RNAs （IncRNAs） participate in the regulation of multiple biological processes via diverse manners, one of which is functioning as endogenous target mimics （eTMs） to modulate microRNAs （miRNAs） by competing for their targets. Previously, we have predicted one IncRNA （osa-eTM160） as an endogenous repressor of osa-miR160 and validated the target mimicry ability of osa-eTM160 for ath-miR160 in Arabidopsis thaliana, yet the functions of osa-eTM160 in rice remain obscure. Here, we demonstrated that osa-eTM160 attenuated the repression of osa-miR160 on osa-ARF18 mRNAs during early anther developmental stages through the target mimicry manner, therefore to regulate rice seed setting and seed size. These findings revealed the roles of osa-eTM160 in rice, and indicated that lncRNAs with eTM functions may serve as temporal regulators to modulate the effects of miRNAs at specific developmental stages.展开更多
基金Work done in Guiliang Tang's lab was supported,in whole or in part,by USDA National Research Initiative grants(2006- 35301-17115 and 2006-35100-17433)the National Science Foundation grants(MCB-0718029:Subaward No.S-00000260 and IOS-1048216/IOS-1219316)+1 种基金the startup of Michigan Technological UniversityWork done in Xiaoqing Tang's lab was supported in part by the National Institutes of Health(K01 DK078648 and R03 DK084166)
文摘MicroRNAs（miRNAs） are a population of highly conserved specific small ribo-regulators that negatively regulate gene expressions in both plants and animals.They play a key role in post-transcriptional gene regulation by destabilizing the target gene transcripts or blocking protein translation from them.Interestingly,these negative regulators are largely compromised by an upstream layer of negative regulators ＂target mimics＂ found in plants or ＂endogenous competing RNAs＂ revealed recently in animals.These endogenous regulatory mechanisms of ＂double negatives making a positive＂ have now been developed into a key strategy in the study of small RNA functions. This review presents some reflections on the long journey to the short tandem target mimic（STTM） for selective destruction/blockage of specific miRNAs in plants and animals,and the potential applications of STTM are discussed.
基金supported by Chinese Scientific and Technological Major Special Project(2014ZX09J13103-01B-003 and 2014ZX09J15104002)
文摘OBJECTIVE To observe the anti-aging effects of SOD mimic AEOL^(-1)0150 in antisenescence accelerated mouse resistant 1(SAMR1)strain.METHODS The lifespan of SAMR1 mice were observed by subcutaneous injection AEOL^(-1)0150 2 mg·kg-1once a week.Morris water maze,new object recognition,nesting and forced swimming were used to observe the behavioral changes of animals.Lymphocyte subgroups and ROS were measured by Flow cytometry.The cytokines levels were determined by Luminex method.The number of DCX+neurons in brain tissue was observed by immunofluorescence.RESULTS The results showed that AEOL^(-1)0150 could prolong the mean lifespan of SAMR1 mice,but it had no obvious effect on maximal lifespan.What′s more,AEOL^(-1)0150 could significantly improve the spatial learning memory of aged mice,but it could not increase the number of DCX+neurons in the hypothalamic MBH and hippocampal DG regions.Then,we observed the effects of AEOL^(-1)0150 on peripheral blood lymphocyte subgroups and cytokines.We found that AEOL^(-1)0150significantly modulated the lymphocyte subgroups and cytokine release.Especially,AEOL^(-1)0150 can dose-dependently inhibit plasma levels of SASP related inflammatory cytokines TNF-αand IL^(-1)7.CONCLUSION The results indicate that AEOL^(-1)0150 has anti-aging effects,and the effects are closely related to modulating immunity and inhibiting SASP production.
基金supported by grants from the National Natural Science Foundation of China(Nos.31140078,and 30972928)
文摘This study examined the expressions of miR-22 and miR-135a in rats with acute edematous pancreatitis （AEP） and their target genes in order to shed light on the involvement of miR-22 and miR-135a in the pathogenesis of acute pancreatitis （AP）. The in vivo model of AEP was established by introperitoneal injection of L-arginine （150 mg/kg） in rats. The miRNA microarray analysis was used to detect the differential expression of miRNAs in pancreatic tissue in AEP and normal rats. The in vitro AEP model was established by inducing the rat pancreatic acinar cell line （AR42J） with 50 ng/mL re- combinant rat TNF-ct. Real-time quantitative RT-PCR was employed to detect the expression of miR-22 and miR-135a in AR42J cells. Lentiviruses carrying the miRNA mimic and anti-miRNA oligonucleotide （AMO） of miR-22 and miR-135a were transfected into the AR42J cells. The AR42J cells transfected with vehicle served as control. Western blotting was used to measure the expression of activated cas- pase3 and flow cytometry analysis to detect the apoptosis of AR42J cells. Targets of miR-22 and miR-135a were predicted by using TargetScan, miRanda, and TarBase. Luciferase reporter assay and quantitative real-time RT-PCR were performed to confirm whether ErbB3 and Ptk2 were the target gene of miR-22 and miR-135a, respectively. The results showed that the expression levels of miR-22 and miR-135a were obviously increased in AEP group compared with the control group in in-vivo and in-vitro models. The expression levels of miR-22 and miR-135a were elevated conspicuously and the expression levels of their target genes were reduced significantly in AR42J cells transfected with Ienti- viruses carrying the miRNA mimic. The apoptosis rate was much higher in the TNF-ct-induced cells than in non-treated cells. The AR42J cells transfected with miRNA AMOs expressed lower level of miR-22 and miR-135a and had lower apoptosis rate, but the expression levels of ErbB3 and Ptk2 were increased obviously. It was concluded that the expression levels of miR-22 and miR-135a were elevated in AEP. Up-regulating the expression of miR-22 and miR-135a may promote the apoptosis of pancreatic acinar cells by repressing ErbB3 and Ptk2 expression in AEP.
基金supported by the National Natural Science Foundation of China(31371318 to M.W.)the State Key Laboratory of Plant Genomics(SKLPG2011B0105 to X.-J.W.)Youth Innovation Promotion Association Grant from Chinese Academy of Sciences(to M.W.)
文摘Long noncoding RNAs （IncRNAs） participate in the regulation of multiple biological processes via diverse manners, one of which is functioning as endogenous target mimics （eTMs） to modulate microRNAs （miRNAs） by competing for their targets. Previously, we have predicted one IncRNA （osa-eTM160） as an endogenous repressor of osa-miR160 and validated the target mimicry ability of osa-eTM160 for ath-miR160 in Arabidopsis thaliana, yet the functions of osa-eTM160 in rice remain obscure. Here, we demonstrated that osa-eTM160 attenuated the repression of osa-miR160 on osa-ARF18 mRNAs during early anther developmental stages through the target mimicry manner, therefore to regulate rice seed setting and seed size. These findings revealed the roles of osa-eTM160 in rice, and indicated that lncRNAs with eTM functions may serve as temporal regulators to modulate the effects of miRNAs at specific developmental stages.
