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Taurine: a promising nutraceutic in the prevention of retinal degeneration 被引量:2
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作者 Diego García-Ayuso Johnny Di Pierdomenico +3 位作者 Ana Martínez-Vacas Manuel Vidal-Sanz Serge Picaud María PVillegas-Pérez 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第3期606-610,共5页
Taurine is considered a non-essential amino acid because it is synthesized by most mammals.However,dietary intake of taurine may be necessary to achieve the physiological levels required for the development,maintenanc... Taurine is considered a non-essential amino acid because it is synthesized by most mammals.However,dietary intake of taurine may be necessary to achieve the physiological levels required for the development,maintenance,and function of certain tissues.Taurine may be especially important for the retina.The concentration of taurine in the retina is higher than that in any other tissue in the body and taurine deficiency causes retinal oxidative stress,apoptosis,and degeneration of photoreceptors and retinal ganglion cells.Low plasma taurine levels may also underlie retinal degeneration in humans and therefore,taurine administration could exert retinal neuroprotective effects.Taurine has antioxidant,anti-apoptotic,immunomodulatory,and calcium homeostasis-regulatory properties.This review summarizes the role of taurine in retinal health and disease,where it appears that taurine may be a promising nutraceutical. 展开更多
关键词 amino acid ANTI-INFLAMMATORY ANTIOXIDANT gamma-aminobutyric acid NUTRACEUTICAL photoreceptor degeneration RETINA retinitis pigmentosa taurine
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Taurine attenuates activation of hepatic stellate cells by inhibiting autophagy and inducing ferroptosis 被引量:1
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作者 Sen Li Qian-Jun Ren +8 位作者 Can-Hao Xie Yang Cui Li-Tao Xu Yi-Dan Wang Su Li Xing-Qiu Liang Bin Wen Ming-Kun Liang Xiao-Fang Zhao 《World Journal of Gastroenterology》 SCIE CAS 2024年第15期2143-2154,共12页
BACKGROUND Liver fibrosis is a compensatory response during the tissue repair process in chronic liver injury,and finally leads to liver cirrhosis or even hepatocellular carcinoma.The pathogenesis of hepatic fibrosis ... BACKGROUND Liver fibrosis is a compensatory response during the tissue repair process in chronic liver injury,and finally leads to liver cirrhosis or even hepatocellular carcinoma.The pathogenesis of hepatic fibrosis is associated with the progressive accumulation of activated hepatic stellate cells(HSCs),which can transdiffer-entiate into myofibroblasts to produce an excess of the extracellular matrix(ECM).Myofibroblasts are the main source of the excessive ECM responsible for hepatic fibrosis.Therefore,activated hepatic stellate cells(aHSCs),the principal ECM producing cells in the injured liver,are a promising therapeutic target for the treatment of hepatic fibrosis.AIM To explore the effect of taurine on aHSC proliferation and the mechanisms involved.METHODS Human HSCs(LX-2)were randomly divided into five groups:Normal control group,platelet-derived growth factor-BB(PDGF-BB)(20 ng/mL)treated group,mmol/L,respectively)with PDGF-BB(20 ng/mL)treated group.Cell Counting Kit-8 method was performed to evaluate the effect of taurine on the viability of aHSCs.Enzyme-linked immunosorbent assay was used to estimate the effect of taurine on the levels of reactive oxygen species(ROS),malondialdehyde,glutathione,and iron concen-tration.Transmission electron microscopy was applied to observe the effect of taurine on the autophagosomes and ferroptosis features in aHSCs.Quantitative real-time polymerase chain reaction and Western blot analysis were performed to detect the effect of taurine on the expression ofα-SMA,Collagen I,Fibronectin 1,LC3B,ATG5,Beclin 1,PTGS2,SLC7A11,and p62.RESULTS Taurine promoted the death of aHSCs and reduced the deposition of the ECM.Treatment with taurine could alleviate autophagy in HSCs to inhibit their activation,by decreasing autophagosome formation,downregulating LC3B and Beclin 1 protein expression,and upregulating p62 protein expression.Meanwhile,treatment with taurine triggered ferroptosis and ferritinophagy to eliminate aHSCs characterized by iron overload,lipid ROS accumu-lation,glutathione depletion,and lipid peroxidation.Furthermore,bioinformatics analysis demonstrated that taurine had a direct targeting effect on nuclear receptor coactivator 4,exhibiting the best average binding affinity of-20.99 kcal/mol.CONCLUSION Taurine exerts therapeutic effects on liver fibrosis via mechanisms that involve inhibition of autophagy and trigger of ferroptosis and ferritinophagy in HSCs to eliminate aHSCs. 展开更多
关键词 Hepatic stellate cells AUTOPHAGY Ferroptosis Molecular docking taurine
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Mitigating diabetes-related complications:Empowering metformin with cholecalciferol and taurine supplementation in type 2 diabetic rats
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作者 Mai S Attia Fadwa Ayman +4 位作者 Mohamed S Attia Galal Yahya Mansour H Zahra Magdi Mohamed Ibrahim Khalil Abdel Aziz A Diab 《World Journal of Diabetes》 SCIE 2024年第8期1778-1792,共15页
BACKGROUND Type 2 diabetes is one of the most prevalent chronic diseases worldwide,significantly impacting patients'quality of life.Current treatment options like metformin(MET)effectively counteract hyperglycemia... BACKGROUND Type 2 diabetes is one of the most prevalent chronic diseases worldwide,significantly impacting patients'quality of life.Current treatment options like metformin(MET)effectively counteract hyperglycemia but fail to alleviate diabetes-associated complications such as retinopathy,neuropathy,nephropathy,hepatopathy,and cardiovascular diseases.AIM To propose the supplementation of cholecalciferol(CHO)and taurine(TAU)to enhance MET efficacy in controlling diabetes while minimizing the risk of associated complications.METHODS The study involved sixty rats,including ten non-diabetic control rats and fifty experimental rats with type 2 diabetes induced by streptozotocin.The experimental rats were further subdivided into positive control and treatment subgroups.The four treatment groups were randomly allocated to a single MET treatment or MET combined with supplements either CHO,TAU,or both.RESULTS Diabetic rats exhibited elevated levels of glucose,insulin,Homeostasis Model Assessment of Insulin Resistance(HOMA-IR),glycated hemoglobin percentage,lipid markers,aspartate aminotransferase,and malondialdehyde,along with reduced levels of antioxidant enzymes(catalase and superoxide dismutase).The administration of CHO and TAU supplements alongside MET in diabetic rats led to a noticeable recovery of islet mass.The antioxidative,anti-inflammatory,and anti-apoptotic properties of the proposed combination therapy significantly ameliorated the aforementioned abnormalities.CONCLUSION The supplementation of CHO and TAU with MET showed the potential to significantly improve metabolic parameters and protect against diabetic complications through its antioxidative,anti-inflammatory,and antiapoptotic effects. 展开更多
关键词 Diabetes complications METFORMIN CHOLECALCIFEROL taurine Glycated hemoglobin% ANTIOXIDANT
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Encapsulating taurine into liposomes:A promising therapeutic for liver fibrosis
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作者 Xue-Juan Zhang Xiao-Yi Jiang +2 位作者 Yi-Lin Ma Fei-Yi Huang Zheng-Wei Huang 《World Journal of Gastroenterology》 SCIE CAS 2024年第41期4509-4513,共5页
We summarize the mechanism by which taurine(Tau)inhibits autophagy and induces iron apoptosis in hepatic stellate cells.Tau interacts with autophagy regulates multifunctional proteins,microtubule-associated protein 1 ... We summarize the mechanism by which taurine(Tau)inhibits autophagy and induces iron apoptosis in hepatic stellate cells.Tau interacts with autophagy regulates multifunctional proteins,microtubule-associated protein 1 light chain 3 Beta,and autophagy-related gene 5 to inhibit autophagy,binds to ferritin heavy chain 1 and nuclear receptor coactivator 4 to trigger ferritin autophagy,and interacts with glutathione peroxidase 4 to promote iron apoptosis.There is a solid rationale for developing Tau-based therapies targeting autophagy and ferroptosis regulation.From a pharmaceutical point of view,there are certain requirements for Tau protein delivery systems,such as loading efficiency,stability,and targeting.Nanomaterials should also contain a hydrophilic motif similar to Tau to optimize loading efficiency.Since Tau is a hydrophilic molecule with high water solubility,liposomes,micelles,and amphiphilic polymer nanoparticles may represent a superior choice.The nanostructure of the liposome includes a water region and a lipid membrane to sequester hydrophilic and hydrophobic drugs,respectively,whereas Tau is expected to be loaded into the water region.In addition,a representative method of actively targeting hematopoietic stem cells is introduced.A Tau-based method for the treatment of liver fibrosis is proposed based on the formulation of common liposomes(lecithin plus cholesterol). 展开更多
关键词 taurin LIPOSOME Liver fibrosis Targeted therapy Nanoparticle delivery systems Hepatic stellate cells
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Emergence of taurine as a therapeutic agent for neurological disorders
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作者 Ashok Jangra Priyanka Gola +13 位作者 Jiten Singh Pooja Gond Swarnabha Ghosh Mahesh Rachamalla Abhijit Dey Danish Iqbal Mehnaz Kamal Punya Sachdeva Saurabh Kumar Jha Shreesh Ojha Dinesh Kumar Niraj Kumar Jha Hitesh Chopra Shing Cheng Tan 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第1期62-68,共7页
Taurine is a sulfur-containing,semi-essential amino acid that occurs naturally in the body.It alternates between inflammation and oxidative stress-mediated injury in various disease models.As part of its limiting func... Taurine is a sulfur-containing,semi-essential amino acid that occurs naturally in the body.It alternates between inflammation and oxidative stress-mediated injury in various disease models.As part of its limiting functions,taurine also modulates endoplasmic reticulum stress,Ca^(2+)homeostasis,and neuronal activity at the molecular level.Taurine effectively protects against a number of neurological disorders,including stro ke,epilepsy,cerebral ischemia,memory dysfunction,and spinal cord injury.Although various therapies are available,effective management of these disorders remains a global challenge.Approximately 30 million people are affected worldwide.The design of taurine fo rmation co uld lead to potential drugs/supplements for the health maintenance and treatment of central nervous system disorders.The general neuroprotective effects of taurine and the various possible underlying mechanisms are discussed in this review.This article is a good resource for understanding the general effects of taurine on various diseases.Given the strong evidence for the neuropharmacological efficacy of taurine in various experimental paradigms,it is concluded that this molecule should be considered and further investigated as a potential candidate for neurotherapeutics,with emphasis on mechanism and clinical studies to determine efficacy. 展开更多
关键词 antioxidant EPILEPSY γ-amino butyric acid neurodegenerative disorders NEUROPROTECTION oxidative stress spinal cord injury taurine
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Flattening the biological age curve by improving metabolic health:to taurine or not to taurine,that’s the question
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作者 Kwok M.Ho Anna Lee +7 位作者 William Wu Matthew T.V.Chan Lowell Ling Jeffrey Lipman Jason Roberts Edward Litton Gavin M.Joynt Martin Wong 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2023年第11期813-823,共11页
The aging population is an important issue around the world especially in developed countries.Although med-ical advances have substantially extended life span,the same cannot be said for the duration of health span.We... The aging population is an important issue around the world especially in developed countries.Although med-ical advances have substantially extended life span,the same cannot be said for the duration of health span.We are seeing in-creasing numbers of elderly people who are frail and/or have multiple chronic conditions;all of these can affect the quality of life of the elderly population as well as increase the burden on the healthcare system.Aging is mechanistically related to common medical conditions such as diabetes mellitus,ischemic heart disease,cognitive decline,and frailty.A recently accepted concept termed‘Accelerated Biological Aging’can be diagnosed when a person’s biological age—as measured by biomarkers of DNA methylation—is older than their corresponding chronological age.Taurine,a conditionally essential amino acid,has received much attention in the past few years.A substantial number of animal studies have provided a strong scientific foundation sug-gesting that this amino acid can improve cellular and metabolic health,including blood glucose control,so much that it has been labelled one of the‘longevity amino acids’.In this review article,we propose the rationale that an adequately powered random-ized-controlled-trial(RCT)is needed to confirm whether taurine can meaningfully improve metabolic and microbiome health,and biological age.This trial should incorporate certain elements in order to provide the much-needed evidence to guide doctors,and also the community at large,to determine whether this promising and inexpensive amino acid is useful in improving human metabolic health. 展开更多
关键词 RATIONAL meaningful taurine
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Metabolomic profiling for identification of metabolites and relevant pathways for taurine in hepatic stellate cells 被引量:4
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作者 Xin Deng Xing-Qiu Liang +3 位作者 Fei-Guo Lu Xiao-Fang Zhao Lei Fu Jian Liang 《World Journal of Gastroenterology》 SCIE CAS 2017年第31期5713-5721,共9页
AIM To develop a reliable and simple method to identify important biological metabolites and relevant pathways for taurine in hepatic stellate cells(HSCs), in order to provide more data for taurine therapy.METHODS All... AIM To develop a reliable and simple method to identify important biological metabolites and relevant pathways for taurine in hepatic stellate cells(HSCs), in order to provide more data for taurine therapy.METHODS All the biological samples were analyzed by using highperformance liquid chromatography-time electrospray ionization/quadrupole-time of flight mass spectrometry. Principal component analysis and partial least squares discriminant analysis were used to identify statistically different metabolites for taurine in HSCs, and metabolomic pathway analysis was used to do pathway analysis for taurine in HSCs. The chemical structure of the related metabolites and pathways was identified by comparing the m/z ratio and ion mode with the data obtained from free online databases.RESULTS A total of 32 significant differential endogenous metabolites were identified, which may be related to the mechanism of action of taurine in HSCs. Among the seven relevant pathways identified, sphingolipid metabolism pathway, glutathione metabolism pathway and thiamine metabolism pathway were found to be the most important metabolic pathways for taurine in HSCs.CONCLUSION This study showed that there were distinct changes in biological metabolites of taurine in HSCs and three differential metabolic pathways including sphingolipid pathway, glutathione pathway and thiamine metabolism pathway might be of key importance in mediating the mechanism of action of taurine in HSCs. 展开更多
关键词 Natural taurine Hepatic stellate cells Pathway High performance liquid chromatographytime electrospray ionization/quadrupole-time of flight mass spectrometry metabolomics
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Cysteine dioxygenase and taurine are essential for embryo implantation by involving in E2-ERαand P_(4)-PR signaling in mouse
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作者 Di Zhang Zhijuan Wang +5 位作者 Xuan Luo Hongzhou Guo Guobin Qiu Yuneng Gong Hongxu Gao Sheng Cui 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第3期1040-1053,共14页
Background Taurine performs multiple physiological functions,and the maintenance of taurine level for most mammals relies on active uptake from diet and endogenous taurine synthesis through its synthesis enzymes,inclu... Background Taurine performs multiple physiological functions,and the maintenance of taurine level for most mammals relies on active uptake from diet and endogenous taurine synthesis through its synthesis enzymes,including cysteine dioxygenase(CDO).In addition,uterus tissue and uterus fluid are rich in to urine,and to urine synthesis is regulated by estrogen(E2)and progesterone(P_(4)),the key hormones priming embryo-uterine crosstalk during embryo implantation,but the functional interactions and mechanisms among which are largely unknown.The present study was thus proposed to identify the effects of CDO and taurine on embryo implantation and related mechanisms by using Cdo knockout(KO)and ovariectomy(OVX)mouse models.Results The uterine CDO expression was assayed from the first day of plugging(d 1)to d 8 and the results showed that CDO expression level increased from d 1 to d 4,followed by a significant decline on d 5 and persisted to d 8,which was highly correlated with serum and uterine taurine levels,and serum P_(4) concentration.Next,Cdo KO mouse was established by CRISPER/Cas9.It was showed that Cdo deletion sharply decreased the taurine levels both in serum and uterus tissue,causing implantation defects and severe subfertility.However,the implantation defects in Cdo KO mice were partly rescued by the taurine supplementation.In addition,Cdo deletion led to a sharp decrease in the expressions of P_(4)receptor(PR)and its responsive genes Ihh,Hoxa10 and Hand2.Although the expression of uterine estrogen receptor(ERa)had no significant change,the levels of ERa induced genes(Muc1,Ltf)during the implantation window were upregulated after Cdo deletion.These accompanied by the suppression of stroma cell proliferation.Meanwhile,E2inhibited CDO expression through ERa and P_(4)upregulated CDO expression through PR.Conclusion The present study firstly demonstrates that taurine and CDO play prominent roles in uterine receptivity and embryo implantation by involving in E2-ERa and P_(4)-PR signaling.These are crucial for our understanding the mechanism of embryo implantation,and infer that taurine is a potential agent for improving reproductive efficiency of livestock industry and reproductive medicine. 展开更多
关键词 CDO E2 Embryo implantation P_(4) taurine
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Gelatin-based hydrogel functionalized with taurinemoieties for in vivo skin tissue regeneration
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作者 Farnaz Rahimi Nima Ahmadkhani +5 位作者 Aida Goodarzi Fariba Noori Sajad Hassanzadeh Sepideh Saghati Mehdi Khanmohammadi Arash Goodarzi 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2023年第3期284-297,共14页
Functionalized hydrogels stimulate the migration and morphogenesis of endothelial cells(ECs)and are useful substrates for wound healing.The present study investigates the feasibility of covalent conjugation of taurine... Functionalized hydrogels stimulate the migration and morphogenesis of endothelial cells(ECs)and are useful substrates for wound healing.The present study investigates the feasibility of covalent conjugation of taurine(Tau)on a gelatin-based hydrogel.This hydrogel is expected to maintain positive charged growth factors such as basic fibroblast growth factor(bFGF)and vascular endothelial growth factors(VEGFs)near ECs within the hydrogel microenvironment.The gelatin was conjugated with hydroxyl phenol(Ph)and Tau moieties,and in following that Ph residues were crosslinked through a horseradish peroxidase-catalyzed reaction.The migration characteristics of ECs were analyzed by scratch migration assay and microparticle-based cell migration assay.Cellular morphology and amounts of angiopoietin 1(Ang 1),bFGF,and VEGF proteins were evaluated for encapsulated cells.The potential of synthesized hydrogels in wound healing was assessed by the percentage of reduction from the original wound size and histopathological analyses of rat skin.The incorporated Tau molecules within the hydrogel remained stable through covalent bonds during incubation.During extended incubation,the gelatin-based hydrogel conjugated with Tau improved the migration distance and number of existing migrated ECs.Immobilized Tau within the gelatin-based hydrogel induced high motility of ECs,accompanied by robust cytoskeleton extension and a cell subpopulation that expressed CD44 and CD31 receptors as well as enhancement of Ang 1,bFGF,and VEGF.We found that injectable Gel-Ph-Tau effectively improves wound-healing parameters. 展开更多
关键词 taurine conjugation Covalent crosslinking Immobilization of growth factors Gelatin-based hydrogel Endothelial cells
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Endometrial Cancer Research Based on Gut Microbiomics and Metabolomics:An Analysis of Correlation and Differences
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作者 Dan Xu Fengqin Xue +3 位作者 Ruifang Zhai Sanyuan Zhang Zhe Wang Peiyue Yu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2024年第10期1204-1207,共4页
Endometrial cancer(EC)is a malignant tumour that occurs in the epithelial cells of the endometrium and represents one of the most common malignancies involving the female reproductive system,with endometrioid adenocar... Endometrial cancer(EC)is a malignant tumour that occurs in the epithelial cells of the endometrium and represents one of the most common malignancies involving the female reproductive system,with endometrioid adenocarcinoma as the most common type.In recent years,with an increasingly aging society and the growing number of obese people,the incidence of EC is constantly rising,posing a serious threat to women’s health.Some studies have reported that the interruption of digestion and absorption caused by imbalance in intestinal microbiota may lead to conditions such as obesity,hypertension,diabetes,and hormone imbalance,which are all risk factors for EC.Meanwhile,intestinal bacteria produce a series of metabolites during colonization and reproduction,which can rapidly respond to changes in the microenvironment of the body.Changes in their types and quantities can serve as sensitive indicators of physiological and pathological changes in the body.Patients with EC often suffer from metabolic diseases,which can lead to metabolic disorders involving carbohydrates,fats,and amino acid in their bodies. 展开更多
关键词 metab CANCER QUANTITIES
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Effects of Taurine on Physiological Properties of Lyium bararum L. Seedlings
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作者 杨涓 康建宏 +1 位作者 谢亚军 张俊华 《Agricultural Science & Technology》 CAS 2008年第6期14-17,共4页
[Objective] The study was to investigate the relationship between taurine and physiological activities of Lyium bararum seedlings. [Method] Through applying different concentrations of taurine(0, 10, 100, 500, 1 000, ... [Objective] The study was to investigate the relationship between taurine and physiological activities of Lyium bararum seedlings. [Method] Through applying different concentrations of taurine(0, 10, 100, 500, 1 000, 5 000 mg/L), the photosynthetic rate(Pn), stomatal conductance(Gs), and the activities of membrane protective enzymes superoxide dismutase(SOD) and peroxidase (POD) in the treated L. bararum seedlings were measured. [Result] Treatment of proper taurine concentration could to some extent enhance Pn and increase the POD and SOD activities, and meanwhile decreased the Gs and MDA content. And the optimum application amount of taurine was determined to be 500 mg/L. [Conclusion] Proper concentration of taurine has protective effects on the cell membrane of L. bararum seedlings. 展开更多
关键词 taurine Lyium bararum SEEDLINGS PHYSIOLOGICAL PROPERTIES
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Taurine牛基因组序列图:反刍动物生物学及其进化论的窗口
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作者 何忠武 杨金旭 《中国畜禽种业》 2010年第10期141-143,共3页
为了理解反刍动物的生物学和进化论,牛的基因组图被测定出有大约7倍区域(Genome sevenfold coverage)。整个序列含有最低限度22000个基因,有一套核心基因14345个,是7个哺乳动物种共享拥有的源祖同源基因或直系同源基因(Ortholog),其中有... 为了理解反刍动物的生物学和进化论,牛的基因组图被测定出有大约7倍区域(Genome sevenfold coverage)。整个序列含有最低限度22000个基因,有一套核心基因14345个,是7个哺乳动物种共享拥有的源祖同源基因或直系同源基因(Ortholog),其中有1217个基因在非真哺乳动物亚纲(有袋目动物或单孔目动物)基因组图中不存在或没被检测到。牛染色体特有的进化演变区的片断复制的密度较高,重复元素丰富,并且这种种类特有的基因变异与产乳和免疫反应有关。表达代谢的基因一般是高度保守的,但是人类跟它们同源的5个参与代谢的源祖同源基因已丢失或广泛地发生了漂移。牛的基因组图谱就这样为理解哺乳动物进化和加速家畜泌乳和产肉的遗传改进提供了一种资源。 展开更多
关键词 taurine 基因组 基因序列 重复序列 动物进化
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Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-Fluoro-7-Nitrobenzofurazan as a Derivatizing Agent 被引量:14
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作者 WANG XiFeng CHI DeFeng +2 位作者 SU GuanMin LI Lin SHAO LiHua 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2011年第5期537-542,共6页
Objective A highly sensitive and rapid high‐performance liquid chromatography method with pre‐column derivatization with 4‐fluoro‐7‐nitrobenzofurazan was developed for determination of taurine in biological sampl... Objective A highly sensitive and rapid high‐performance liquid chromatography method with pre‐column derivatization with 4‐fluoro‐7‐nitrobenzofurazan was developed for determination of taurine in biological samples,including plasma,brain,and liver.Methods The optimum derivatization reaction temperature was 70℃,and at this temperature the reaction was complete within 3 min.The derivatized taurine was separated using phosphate buffer (0.02 mol/L,pH 6.0):acetonitrile (84:16,v/v) as the mobile phase at a flow rate of 1.0 mL/min,and a column temperature of 25℃.The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.Results The intra‐ and the inter‐day coefficients of variation for the method were 5.3% and 7.7%,respectively.The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.Conclusion This method can be used to determine the taurine contents in plasma,brain,and liver from normal rats and human plasma. 展开更多
关键词 taurine HPLC 4‐fluoro‐7‐nitrobenzofurazan DERIVATIZATION
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Natural taurine promotes apoptosis of human hepatic stellate cells in proteomics analysis 被引量:13
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作者 Deng, Xin Liang, Jian +3 位作者 Lin, Zhi-Xiu Wu, Fa-Sheng Zhang, Ya-Ping Zhang, Zhi-Wei 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第15期1916-1923,共8页
AIM:To study the differential expression of proteins between natural taurine treated hepatic stellate cells and controls, and investigate the underlying regulatory mechanism of natural taurine in inhibiting hepatic fi... AIM:To study the differential expression of proteins between natural taurine treated hepatic stellate cells and controls, and investigate the underlying regulatory mechanism of natural taurine in inhibiting hepatic fibrosis.METHODS: A proteomic strategy combining two-dimensional gel electrophoresis and ultraperform ance liquid chromatographyelectrospray ionizationtandem mass spectrometry (UPLCESIMS/MS) was used to study the differential expression of proteins and Western blotting was used to validate the results. Gene ontology (GO) method was utilized to analyze the functional enrichment of differentially expressed proteins. Flow cytometry was performed to compare the apoptosis rate between taurinetreated and untreated hepatic stellate cells (HSCs).RESULTS: Nineteen differentially expressed proteins (11 upregulated and 8 downregulated) were identifiedby 2D/MS, and the expression profiles of GLO1 and ANXA1 were validated by Western blotting. GO analysis found that these differentially expressed proteins were enriched within biological processes such as "cellular apoptosis", "oxidation reaction" and "metabolic process" in clusters. Flow cytometric analysis showed that taurinetreated HSCs had a significantly increased apoptosis rate when compared with the control group.CONCLUSION: Natural taurine can promote HSC apoptosis so as to inhibit hepatic fibrosis. 展开更多
关键词 Natural taurine PROTEOMICS Hepatic stellate cell Hepatic f ibrosis APOPTOSIS
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Integrated network analysis of transcriptomic and protein-protein interaction data in taurine-treated hepatic stellate cells 被引量:6
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作者 Xing-Qiu Liang Jian Liang +2 位作者 Xiao-Fang Zhao Xin-Yuan Wang Xin Deng 《World Journal of Gastroenterology》 SCIE CAS 2019年第9期1067-1079,共13页
BACKGROUND Studies show that the antifibrotic mechanism of taurine may involve its inhibition of the activation and proliferation of hepatic stellate cells(HSCs). Since the molecular mechanism of taurine-mediated anti... BACKGROUND Studies show that the antifibrotic mechanism of taurine may involve its inhibition of the activation and proliferation of hepatic stellate cells(HSCs). Since the molecular mechanism of taurine-mediated antifibrotic activity has not been fully unveiled and is little studied, it is imperative to use "omics" methods to systematically investigate the molecular mechanism by which taurine inhibits liver fibrosis.AIM To establish a network including transcriptomic and protein-protein interaction data to elucidate the molecular mechanism of taurine-induced HSC apoptosis.METHODS We used microarrays, bioinformatics, protein-protein interaction(PPI) network,and sub-modules to investigate taurine-induced changes in gene expression in human HSCs(LX-2). Subsequently, all of the differentially expressed genes(DEGs) were subjected to gene ontology function and Kyoto encyclopedia of genes and genomes pathway enrichment analysis. Furthermore, the interactions of DEGs were explored in a human PPI network, and sub-modules of the DEGs interaction network were analyzed using Cytoscape software.RESULTS A total of 635 DEGs were identified in taurine-treated HSCs when compared with the controls. Of these, 304 genes were statistically significantly up-regulated, and 331 down-regulated. Most of these DEGs were mainly located on the membrane and extracellular region, and are involved in the biological processes of signal transduction, cell proliferation, positive regulation of extracellular regulated protein kinases 1(ERK1) and ERK2 cascade, extrinsic apoptotic signaling pathway and so on. Fifteen significantly enriched pathways with DEGs were identified, including mitogen-activated protein kinase(MAPK) signaling pathway, peroxisome proliferators-activated receptor signaling pathway,estrogen signaling pathway, Th1 and Th2 cell differentiation, cyclic adenosine monophosphate signaling pathway and so on. By integrating the transcriptomics and human PPI data, nine critical genes, including MMP2, MMP9, MMP21,TIMP3, KLF10, CX3CR1, TGFB1, VEGFB, and EGF, were identified in the PPI network analysis.CONCLUSION Taurine promotes the apoptosis of HSCs via up-regulating TGFB1 and then activating the p38 MAPK-JNK-Caspase9/8/3 pathway. These findings enhance the understanding of the molecular mechanism of taurine-induced HSC apoptosis and provide references for liver disorder therapy. 展开更多
关键词 taurine Hepatic stellate cells DIFFERENTIALLY EXPRESSED genes Liver FIBROGENESIS TRANSCRIPTOMIC PROTEIN-PROTEIN interaction network
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Taurine protects against retinal and optic nerve damage induced by endothelin-1 in rats via antioxidant effects 被引量:3
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作者 Natasha Najwa Nor Arfuzir Renu Agarwal +3 位作者 Igor Iezhitsa Puneet Agarwal Sabrilhakim Sidek Nafeeza Mohd Ismail 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第11期2014-2021,共8页
Endothelin-1(ET-1), a potent vasoconstrictor, is involved in retinal vascular dysregulation and oxidative stress in glaucomatous eyes. Taurine(TAU), a naturally occurring free amino acid, is known for its neuropro... Endothelin-1(ET-1), a potent vasoconstrictor, is involved in retinal vascular dysregulation and oxidative stress in glaucomatous eyes. Taurine(TAU), a naturally occurring free amino acid, is known for its neuroprotective and antioxidant properties. Hence, we evaluated its neuroprotective properties against ET-1 induced retinal and optic nerve damage. ET-1 was administered intravitreally to Sprague-Dawley rats and TAU was injected as pre-, co-or post-treatment. Animals were euthanized seven days post TAU injection. Retinae and optic nerve were examined for morphology, and were also processed for caspase-3 immunostaining. Retinal redox status was estimated by measuring retinal superoxide dismutase, catalase, glutathione, and malondialdehyde levels using enzyme-linked immuosorbent assay. Histopathological examination showed significantly improved retinal and optic nerve morphology in TAU-treated groups. Morphometric examination showed that TAU pre-treatment provided marked protection against ET-1 induced damage to retina and optic nerve. In accordance with the morphological observations, immunostaining for caspase showed a significantly lesser number of apoptotic retinal cells in the TAU pre-treatment group. The retinal oxidative stress was reduced in all TAU-treated groups, and particularly in the pre-treatment group. The findings suggest that treatment with TAU, particularly pre-treatment, prevents apoptosis of retinal cells induced by ET-1 and hence prevents the changes in the morphology of retina and optic nerve. The protective effect of TAU against ET-1 induced retinal and optic nerve damage is associated with reduced retinal oxidative stress. 展开更多
关键词 ENDOTHELIN-1 RETINA optic nerve taurine oxidative stress
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Selective depression action of taurine in flotation separation of specularite and chlorite 被引量:10
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作者 Mingyang Li Cheng Yang +4 位作者 Zhaoyang Wu Xiangpeng Gao Xiong Tong Xiankun Yu Hongming Long 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2022年第3期637-644,共8页
Chlorite,as the most representative gangue mineral associated with specularite,of which the separation of these two minerals is difficult.This paper investigated the depression effect of taurine on specularite/chlorit... Chlorite,as the most representative gangue mineral associated with specularite,of which the separation of these two minerals is difficult.This paper investigated the depression effect of taurine on specularite/chlorite separation via flotation experiments,adsorption tests,contact angle measurements,Zeta potential detection,FT-IR measurements,and XPS analyses.The results of single mineral flotation indicated that chlorite could be depressed selectively by taurine with the recovery of less than 30%,but the floatability of specularite remains high with recovery rate of 81.77%at pH 10.The artificial mixed mineral flotation results confirmed the effectiveness of taurine as a depressant.Surface adsorption,contact angle,and Zeta potential detection revealed taurine primarily adsorbs on the chlorite surface,which hampered the DDA’s subsequent adsorption and results in the chlorite’s poor floatability.The FT-IR spectra and XPS analyses provided further proof that taurine adsorbed on chlorite surface as an electron donor,and part of the electrons transferred from the sulfonic acid group of taurine to metal ions during the adsorption process.In addition,the hydrogen bond between amino-group of taurine and O ions in chlorite surface was also formed in the adsorption process.Finally,optimized adsorption configurations of taurine on chlorite surfaces were proposed. 展开更多
关键词 FLOTATION taurine SPECULARITE CHLORITE Iron-containing Silicates Depressant
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Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction 被引量:9
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作者 Jing Liu Xiaofeng Wang +3 位作者 Ying Liu Na Yang Jing Xu Xiaotun Ren 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第23期2190-2197,共8页
From pregnancy to parturition, Sprague-Dawley rats were daily administered a low protein diet to establish a model of intrauterine growth restriction. From the 12th day of pregnancy, 300 mg/kg taurine was daily added ... From pregnancy to parturition, Sprague-Dawley rats were daily administered a low protein diet to establish a model of intrauterine growth restriction. From the 12th day of pregnancy, 300 mg/kg taurine was daily added to food until spontaneous delivery occurred. Brain tissues from normal neonatal rats at 6 hours after delivery, neonatal rats with intrauterine growth restriction, and neo- natal rats with intrauterine growth restriction undergoing taurine supplement were obtained for fur- ther experiments. The terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling assay revealed that the number of apoptotic cells in the brain tissue of neonatal rats with intrauterine growth restriction significantly increased. Taurine supplement in pregnant rats reduced cell apoptosis in brain tissue from neonatal rats with intrauterine growth restriction. Immu- nohistochemical staining revealed that taurine supplement increased glial cell line-derived neuro- trophic factor expression and decreased caspase-3 expression in the cerebral cortex of intrauterine growth-restricted fetal rats. These results indicate that taurine supplement reduces cell apoptosis through the glial cell line-derived neurotrophic factor-caspase-3 signaling pathway, resulting in a protective effect on the intrauterine growth-restricted fetal rat brain. 展开更多
关键词 neural regeneration intrauterine growth restriction fetal rats brain neural cells taurine cell apop-tosis glial cell line-derived neurotrophic factor caspase-3 neural development grants-supportedpaper NEUROREGENERATION
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Taurine、EGCG和genistein联合对体外活化肝星状细胞自噬的影响 被引量:4
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作者 宋鹏书 潘火珍 廖明 《中国病理生理杂志》 CAS CSCD 北大核心 2018年第10期1895-1899,共5页
目的:探讨牛磺酸(taurine)、表没食子儿茶素没食子酸酯(EGCG)和三羟基异黄酮(genistein) 3种抗肝纤维化药物联合使用对体外活化大鼠肝星状细胞(HSCs)自噬的影响及可能机制。方法:体外培养大鼠肝星状细胞株HSC-T6,设立对照组、联合药物(t... 目的:探讨牛磺酸(taurine)、表没食子儿茶素没食子酸酯(EGCG)和三羟基异黄酮(genistein) 3种抗肝纤维化药物联合使用对体外活化大鼠肝星状细胞(HSCs)自噬的影响及可能机制。方法:体外培养大鼠肝星状细胞株HSC-T6,设立对照组、联合药物(taurine+EGCG+genistein,TEG)组、自噬抑制剂巴弗洛霉素A1(Baf-A1)组和联合药物+抑制剂(TEG+Baf-A1)组。采用CCK-8法分别检测各组HSCs的存活率,应用透射电镜观察HSCs内部自噬体超微结构变化,通过吖啶橙(AO)染色和荧光显微镜观察各组细胞内自噬溶酶体变化,Western blot分析自噬标志性蛋白LC3-Ⅱ和beclin-1的表达。结果:与对照组相比,TEG组、Baf-A1组和TEG+Baf-A1组细胞的存活率明显降低(P <0. 05);透射电镜观察到细胞内部出现双层或多层膜结构的自噬体,以及单层膜结构的自噬溶酶体。与对照组相比,AO染色结果显示TEG组和Baf-A1组红色荧光区域显著缩小,TEG组中LC3-Ⅱ和beclin-1的表达量显著降低(P <0. 05)。结论:活化的HSCs发生自噬现象,联合药物TEG可阻断HSCs的自噬过程,其作用机制可能与抑制HSCs中自噬体的生成有关。 展开更多
关键词 牛磺酸 表没食子儿茶素没食子酸酯 三羟基异黄酮 肝星状细胞 自噬 肝纤维化
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Attenuation of portal hypertension by natural taurine in rats with liver cirrhosis 被引量:9
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作者 Jian Liang Xin Deng +2 位作者 Zhi-Xiu Lin Li-Chun Zhao Xi-Liu Zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第36期4529-4537,共9页
AIM: To investigate the inhibitory effect of natural taurine (NTau) on portal hypertension (PHT) in rats with experimentally-induced liver cirrhosis (LC). METHODS: Experimentally-induced LC Wistar rats (20 ra... AIM: To investigate the inhibitory effect of natural taurine (NTau) on portal hypertension (PHT) in rats with experimentally-induced liver cirrhosis (LC). METHODS: Experimentally-induced LC Wistar rats (20 rats/group) were treated with either oral saline or oral NTau for 6 consecutive weeks. Evaluation parameters included portal venous pressure (PVP), portal venous resistance (PVR), portal venous flow (PVF), splanchnic vascular resistance (SVR) and mean arterial pressure (NAP). Vasoactive substance levels including nitric oxide (NO), nitric oxide synthase (NOS) and cyclic guanosine monophosphate (cGMP) were also measured. Histological investigation of type Ⅰ and Ⅲ collagen (COL Ⅰ and Ⅲ) and transforming growth factor-β1 (TGF-β1) was also performed. RESULTS: Treatment with NTau (1) significantly decreased PVP, PVR and PVF, and increased MAP and SVP; (2) markedly increased the vascular compliance and reduced the zero-stress of the portal vein; (3) markedly decreased the amount of NO and cGMP and activity of NOS; and (4) improved the pathological status of the liver tissue and reduced the expression of COL Ⅰ, COL Ⅲ and TGF-β1. CONCLUSION: NTau inhibited the LC-induced PHT by improving hyperdynamic circulation, morphology of liver and biomechanical properties of the portal vein in experimentally-induced LC rats. 展开更多
关键词 taurine Liver cirrhosis Portal hypertension RAT
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